不同种子处理方法对两种黄芪种子萌发的影响
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摘要
为提高黄芪种子的发芽率,为黄芪的引种栽培提供参考,对膜荚黄芪与蒙古黄芪两种黄芪种子进行硫酸浸泡、沸水浸泡和打磨3种方法处理,测定两种黄芪发芽率、发芽势及发芽指数,研究不同种子处理方法对膜荚黄芪与蒙古黄芪种子萌发的影响。结果表明:膜荚黄芪硫酸浸泡4min的发芽率、发芽势及发芽指数最高,分别为59%、52%、26.08,硫酸浸泡4min的发芽率与硫酸浸泡3min、沸水浸泡30s及对照对照差异显著,与其余处理差异不显著;蒙古黄芪打磨处理的发芽率、发芽势及发芽指数最高,分别为76%、61%、30.93,打磨处理发芽率与其他处理间差异显著。两种黄芪的发芽率、发芽势及发芽指数综合表现为打磨处理>沸水处理>硫酸处理。
In order to improve the germination rate of Astragalus membranaceus seeds,and provide references for the introduction and cultivation of A.membranaceus,three methods were used to treat two kinds of A.membranaceus seeds,including sulfuric acid immersion,boiling water immersion and grinding.The germination rate,germination potential and germination index of two A.membranaceus cultivars were determined,thus to study the effects of different seed treatment methods on seed germination of A.membranaceus and A.membranaceus var.mongholicus.Results:Germination rate,germination potential and germination index of A.membranaceus seeds immersed in sulfuric acid for 4 min were the highest,59%,52%and 26.08 respectively,of which the germination rate was significantly different with sulfuric acid immersion for 3 min,boiling water immersion for 30 s,and CK,while not significantly different with other treatments.The germination rate,germination potential and germination index of A.membranaceus var.mongholicus grinding were the highest,76%,61%and 30.93 respectively,of which the germination rate was significantly different from other treatments.The germination rate,germination potential and germination index of the two cultivars followed the sequence of grinding treatment > boiling water treatment>sulfuric acid treatment.
In order to improve the germination rate of Astragalus membranaceus seeds,and provide references for the introduction and cultivation of A.membranaceus,three methods were used to treat two kinds of A.membranaceus seeds,including sulfuric acid immersion,boiling water immersion and grinding.The germination rate,germination potential and germination index of two A.membranaceus cultivars were determined,thus to study the effects of different seed treatment methods on seed germination of A.membranaceus and A.membranaceus var.mongholicus.Results:Germination rate,germination potential and germination index of A.membranaceus seeds immersed in sulfuric acid for 4 min were the highest,59%,52%and 26.08 respectively,of which the germination rate was significantly different with sulfuric acid immersion for 3 min,boiling water immersion for 30 s,and CK,while not significantly different with other treatments.The germination rate,germination potential and germination index of A.membranaceus var.mongholicus grinding were the highest,76%,61%and 30.93 respectively,of which the germination rate was significantly different from other treatments.The germination rate,germination potential and germination index of the two cultivars followed the sequence of grinding treatment > boiling water treatment>sulfuric acid treatment.
引文
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[8]郭瑜瑞.不同矿质元素处理下蒙古黄芪生长和有效成分的差异研究[D].咸阳:西北农林科技大学,2014.
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[13]李树娜.栽植密度与播种时期对蒙古黄芪生长发育及次生代谢产物积累的影响[D].咸阳:西北农林科技大学,2015.
[14]高星.沙地黄芪规范化栽培综合农艺措施集成[D].咸阳:西北农林科技大学,2017.
[15]赵锐明,孙连虎,郭凤霞,等.移栽密度对红芪生长发育及药材产量和质量的影响[J].草业学报,2014,23(3):167-174.
[16]赵敏,王炎.膜荚黄芪种子萌发抑制物质特性的初步研究[J].中草药,2001,32(7):643-646.
[17]常晖,张小燕,张跃进,等.黄芪种子内源抑制物质的初步研究[J].种子,2015,34(1):44-47.
[18]张博,石国玺.不同处理对蒙古黄芪种子萌发的影响[J].中兽医医药杂志,2016,35(2):11-14.
[19]程瑶,方向文,蒋志荣,等.温水浸种对蒙古黄芪种子萌发特性的影响[J].植物科学学报,2017,35(3):413-420.
[20]苏洪宇.中药黄芪的栽培技术[J].饲料博览,2017(6):51.
[21]张延红,李金田,卢有媛,等.黄芪种子发芽检验标准化研究[J].中国药房,2016,27(7):998-1001.
[22]许陶瑜,郭淑红,田洪岭,等.不同预处理及光照条件对黄芪种子萌发的影响[J].山西农业科学,2018,46(2):196-198.
[2]胡艳改,杜宇忠,郝磊,等.中药材蒙古黄芪与膜荚黄芪优质种源的研究[J].山西农业科学,2017,45(10):1662-1666,1690.
[3]喻正坤,刘星堦,戴稼禾,等.黄芪改善红细胞变形能力的活性成分研究[J].天然产物研究与开发,1994(2):1-5.
[4]刘志强,李全志,秦贵军.黄芪注射液对早期糖尿病肾病患者血小板功能和血浆内皮素的影响[J].中国中西医结合杂志,2001(4):274-276.
[5]涂继辉.东北产药用黄芪的质量与分类研究[D].沈阳:沈阳药科大学,2006.
[6]袁昌齐.天然药物资源开发与利用:第1版[M].南京:江苏科技出版社,2001.
[7]沈晓峰,房新志,宋东.黄芪在皮肤病治疗中的应用[J].新疆中医药,2000(2):59-60.
[8]郭瑜瑞.不同矿质元素处理下蒙古黄芪生长和有效成分的差异研究[D].咸阳:西北农林科技大学,2014.
[9]姜波.种子包衣后黄芪生长性状与药材品质的研究[D].哈尔冰:黑龙江中医药大学,2009.
[10] HAMZAH A,BEH C.W,SARMANI S.B,et al.2004.Studies on elemental analysis of Chinese traditional herbs by neutron activatio technique and their mutagenic effect[J].Journal of Radioanalytical and Nuclear Chemistry,259(3):499-503.
[11]索风梅,丁万隆,谢彩香,等.蒙古黄芪的生态适宜性数值分析[J].世界科学技术(中医药现代化),2010,12(3):480-485.
[12]冯学金,刘根科,梁素明.蒙古黄芪种质资源研究进展[J].山西农业科学,2010,38(8):95-98.
[13]李树娜.栽植密度与播种时期对蒙古黄芪生长发育及次生代谢产物积累的影响[D].咸阳:西北农林科技大学,2015.
[14]高星.沙地黄芪规范化栽培综合农艺措施集成[D].咸阳:西北农林科技大学,2017.
[15]赵锐明,孙连虎,郭凤霞,等.移栽密度对红芪生长发育及药材产量和质量的影响[J].草业学报,2014,23(3):167-174.
[16]赵敏,王炎.膜荚黄芪种子萌发抑制物质特性的初步研究[J].中草药,2001,32(7):643-646.
[17]常晖,张小燕,张跃进,等.黄芪种子内源抑制物质的初步研究[J].种子,2015,34(1):44-47.
[18]张博,石国玺.不同处理对蒙古黄芪种子萌发的影响[J].中兽医医药杂志,2016,35(2):11-14.
[19]程瑶,方向文,蒋志荣,等.温水浸种对蒙古黄芪种子萌发特性的影响[J].植物科学学报,2017,35(3):413-420.
[20]苏洪宇.中药黄芪的栽培技术[J].饲料博览,2017(6):51.
[21]张延红,李金田,卢有媛,等.黄芪种子发芽检验标准化研究[J].中国药房,2016,27(7):998-1001.
[22]许陶瑜,郭淑红,田洪岭,等.不同预处理及光照条件对黄芪种子萌发的影响[J].山西农业科学,2018,46(2):196-198.