萱草属植物短缩茎组织培养及植株再生
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摘要
[目的]通过诱导不定芽途径,建立萱草属植物短缩茎组培快繁体系。[方法]以‘大同黄花’短缩茎为主要试验材料,研究了不同取样时期下,10个不同浓度6-BA、NAA组合对萱草属植物不定芽诱导、不定芽增殖、生根等组培效果的影响。[结果]‘大同黄花’短缩茎组培最佳取样时期为萌芽期(2～3月下旬);不定芽诱导最佳培养基:MS+1.0mg/L 6-BA+0.1mg/L NAA,诱导率最高为88.89%,诱导系数最高为6.67;不定芽增殖培养基:MS+2.0mg/L6-BA+0.5mg/L NAA;不定芽3～5cm时,接种于生根培养基MS+0.25mg/L NAA中,生根数最多(4.33根/株)。利用上述不定芽诱导培养基,接种了14份萱草属植物的短缩茎,12份材料可诱导出不定芽。[结论]本研究构建了萱草属植物短缩茎组织培养和植株再生体系,扩展了萱草属植物组织培养的外植体选择,对萱草属植物组织培养提供了技术保障。
[Objective]This study was aimed to construct a rapid propagation system with the dwarf stem of the Hemerocallis by inducing the adventitious bud pathway into plants.[Methods]Datong Huanghua was selected as the research material,and the effects of 6-BA and NAA combination at 10 different concentrations on the induction rate of adventitious bud,adventitious bud proliferation,and rooting rate of Hemerocallis were studied at different sampling time.[Results]The results showed that the best sampling time for short-stem culture of'Datong Huanghua'was the germination stage(February to late March).The optimal medium for adventitious bud induction was the mixture of MS plus 1.0 mg/L 6-BA and 0.1 mg/L NAA which produced the highest induction rate at 88.89%,with the highest induction coefficient at 6.67.The adventitious bud proliferation medium was determined as MS combined with 2.0 mg/L 6-BA and 0.5 mg/L NAA.When the adventitious bud was at 3～5 cm length,and was inoculated into the rooting medium of MS+0.25 mg/L NAA,the highest root number was measured as 4.33 per plant.When 14 short stems of the genus Hemerocallis were inoculated into the adventitious bud induction medium as described previously,adventitious buds were induced in 12 out of 14 testing material.[Conclusion]In this study,the tissue culture and plant regeneration system of the dwarf stem of Hemerocallis were developed,and the explant selection of the tissue culture of Hemerocallis was extended,which provided technical support for the annual tissue culture of Hemerocallis.
[Objective]This study was aimed to construct a rapid propagation system with the dwarf stem of the Hemerocallis by inducing the adventitious bud pathway into plants.[Methods]Datong Huanghua was selected as the research material,and the effects of 6-BA and NAA combination at 10 different concentrations on the induction rate of adventitious bud,adventitious bud proliferation,and rooting rate of Hemerocallis were studied at different sampling time.[Results]The results showed that the best sampling time for short-stem culture of'Datong Huanghua'was the germination stage(February to late March).The optimal medium for adventitious bud induction was the mixture of MS plus 1.0 mg/L 6-BA and 0.1 mg/L NAA which produced the highest induction rate at 88.89%,with the highest induction coefficient at 6.67.The adventitious bud proliferation medium was determined as MS combined with 2.0 mg/L 6-BA and 0.5 mg/L NAA.When the adventitious bud was at 3～5 cm length,and was inoculated into the rooting medium of MS+0.25 mg/L NAA,the highest root number was measured as 4.33 per plant.When 14 short stems of the genus Hemerocallis were inoculated into the adventitious bud induction medium as described previously,adventitious buds were induced in 12 out of 14 testing material.[Conclusion]In this study,the tissue culture and plant regeneration system of the dwarf stem of Hemerocallis were developed,and the explant selection of the tissue culture of Hemerocallis was extended,which provided technical support for the annual tissue culture of Hemerocallis.
引文
[1]The American Hemerocallis Society.The American Hemerocallis Society Online Daylily Datebas[DB].[2018-7-24].http://www.daylilies.org/DaylilyDB/search.php.
[2]解有利,陈兰芬,石进朝.大花萱草组织培养研究[J].北京农业职业学院学报,2007,21(5):25-27.
[3]杨丽莉,张晓,晋凡生,等.激素对萱草组织培养参数的影响[J].山西农业科学,2012,40(8):815-818.
[4]李淑英,张颖,晁慧娟,等.大花萱草品种红宝石快繁体系的建立[J].农业工程技术,2015(2):36-38.
[5]杨乃博.杂种大花萱草的试管繁殖[J].植物生理学通讯,1985(5):39.
[6]周朴华,何立珍.黄花莱不同外植体形成的愈伤组织再生苗观察[J].武汉植物学研究,1993,11(3):253-258.
[7]褚焕宁.萱草属植物快繁体系构建及杂种胚拯救研究[D].太谷:山西农业大学,2016.
[8]胡峰,施琼,黄烈健,等.黑木相思愈伤组织诱导及植株再生[J].植物学报,2014,49(5):603-610.
[9]佘启惠,姜媛媛,杨程,等.雪胆属植物的组织培养与栽培研究进展[J].基因组学与应用生物学,2018,37(3):1370-1375.
[10]王凤翱,范鸿芝,胡继金,等.黄花菜组织培养快速繁殖技术与球状体解剖观察的研究[J].湖南农业科学,1987(3):22-23.
[11]范银燕,崔根芳.黄花菜无性系快速繁殖技术研究[J].山西农业科学,1994,22(4):22-24.
[12]刘小英,江华波,铁曼曼,等.黄花菜组织快繁及高效栽培技术综述[J].现代农业科技,2015(4):109-111.
[13]张伟丽,金欣庆.大花萱草新品种‘奶油卷’的组织培养和生产应用[J].植物生理学通讯,2007,43(1):129-130.
[14]彭广霖,姜宁,潘仕梅,等.大花萱草的花梗组织培养与快速繁殖[J].安徽农业科学,2012,40(17):9203-9205.
[15]王紫珊,王广东,王雁,等.多肉植物白银寿‘奇迹’的离体培养与快速繁殖[J].基因组学与应用生物学,2014,33(6):1329-1335.
[2]解有利,陈兰芬,石进朝.大花萱草组织培养研究[J].北京农业职业学院学报,2007,21(5):25-27.
[3]杨丽莉,张晓,晋凡生,等.激素对萱草组织培养参数的影响[J].山西农业科学,2012,40(8):815-818.
[4]李淑英,张颖,晁慧娟,等.大花萱草品种红宝石快繁体系的建立[J].农业工程技术,2015(2):36-38.
[5]杨乃博.杂种大花萱草的试管繁殖[J].植物生理学通讯,1985(5):39.
[6]周朴华,何立珍.黄花莱不同外植体形成的愈伤组织再生苗观察[J].武汉植物学研究,1993,11(3):253-258.
[7]褚焕宁.萱草属植物快繁体系构建及杂种胚拯救研究[D].太谷:山西农业大学,2016.
[8]胡峰,施琼,黄烈健,等.黑木相思愈伤组织诱导及植株再生[J].植物学报,2014,49(5):603-610.
[9]佘启惠,姜媛媛,杨程,等.雪胆属植物的组织培养与栽培研究进展[J].基因组学与应用生物学,2018,37(3):1370-1375.
[10]王凤翱,范鸿芝,胡继金,等.黄花菜组织培养快速繁殖技术与球状体解剖观察的研究[J].湖南农业科学,1987(3):22-23.
[11]范银燕,崔根芳.黄花菜无性系快速繁殖技术研究[J].山西农业科学,1994,22(4):22-24.
[12]刘小英,江华波,铁曼曼,等.黄花菜组织快繁及高效栽培技术综述[J].现代农业科技,2015(4):109-111.
[13]张伟丽,金欣庆.大花萱草新品种‘奶油卷’的组织培养和生产应用[J].植物生理学通讯,2007,43(1):129-130.
[14]彭广霖,姜宁,潘仕梅,等.大花萱草的花梗组织培养与快速繁殖[J].安徽农业科学,2012,40(17):9203-9205.
[15]王紫珊,王广东,王雁,等.多肉植物白银寿‘奇迹’的离体培养与快速繁殖[J].基因组学与应用生物学,2014,33(6):1329-1335.