中药有效组分“配伍艺术”的研究基础--组分配比研究策略
|
摘要
中药配伍包括药材配伍、有效部位配伍和有效成分配伍3个层次,相比于药材配伍,有效部位、有效成分之间的配伍更易于阐明中药药效物质基础及作用机制,成为中药配伍研究的突破点,为中药精准医疗体系下的精准用药提供了新的方法,势必将成为中药精准化进程的重要推动力。而有效部位、有效成分配伍隶属于中药有效组分配伍的范畴,为进一步探讨中药有效组分的"配伍艺术",本文从中药有效组分的配伍模式、配伍组分筛选方法、配伍组分剂量的选择、配比实验设计方法和组分配比优化研究等5个方面来阐述中药组分配伍研究策略。
The compatibility of traditional Chinese medicines(TCM) includes the compatibility of single herb,effective parts and the effective ingredients of TCM herbs. Compared with the compatibility of Chinese herbal medicine, the compatibility between effective parts and effective ingredients of TCM has become a breakthrough point in the study of compatibility. It provides a new method for precise medication research of TCM, which is bound to become an important driving force in the process of precision medicine research. In this paper, we elaborate the strategy of the compatibility and proportion in TCM components from five aspects: the compatibility mode of effective components in TCM, the screening method of compatibility of components in TCM, the selection of compatibility component dose, the design method of proportion test and the optimization research of compatibility and proportion.
The compatibility of traditional Chinese medicines(TCM) includes the compatibility of single herb,effective parts and the effective ingredients of TCM herbs. Compared with the compatibility of Chinese herbal medicine, the compatibility between effective parts and effective ingredients of TCM has become a breakthrough point in the study of compatibility. It provides a new method for precise medication research of TCM, which is bound to become an important driving force in the process of precision medicine research. In this paper, we elaborate the strategy of the compatibility and proportion in TCM components from five aspects: the compatibility mode of effective components in TCM, the screening method of compatibility of components in TCM, the selection of compatibility component dose, the design method of proportion test and the optimization research of compatibility and proportion.
引文
[1]Zhang BL,Wang YY.Basic research on key scientific problems of prescription-developing modern traditional Chinese medicine by compatibility of effective components[J].Chin J Nat Med(中国天然药物),2005,3:258-261.
[2]Liu LM,Chen X,Yue GX,et al.Some thoughts on definition of"Chinese materia medica components"[J].Chin Tradit Herb Drugs(中草药),2018,49:2489-2495.
[3]Shang HC,Zhang BL,Gao XM.Study on compatibility of prescription[J].Chin J Inf Tradit Chin Med(中国中医药信息杂志),2002,9:6-9.
[4]Zhao HY,Zhang CL,Liu XY,et al.Study on the proportion of active ingredients in drug combination of"Suang-Huang-TongGuang-Xin-Pian"[J].Lishizhen Med Mater Med Res(时珍国医国药),2018,29:45-48.
[5]Zhou MM,Hong YL,Lin X,et al.Recent pharmaceutical evidence on the compatibility rationality of traditional Chinese medicine[J].J Ethnopharmacol,2017,206:363-375.
[6]Zhang Y,Wu XD,Yang Y.Role of traditional compatibility and components compatibility under the background of precision medicine[J].Acta Chin Med Pharmacol(中医药学报),2018,46:7-10.
[7]Duan Y,Pei K,Cai H,et al.A new strategy for exploring herb-pair investigation with a case study on Astragali Radix-Corni Fructus in treatment of diabetic nephropathy[J].China J Chin Mater Med(中国中药杂志),2016,41:3919-3926.
[8]Du H,Wang K,Su L,et al.Metabonomic identification of the effects of the Zhimu-Baihe saponins on a chronic unpredictable mild stress-induced rat model of depression[J].J Pharm Biomed Anal,2016,128:469-479.
[9]Xu HQ,Nong WH,Liu CD,et al.The effect of the best compatibility of components in Fruetus Corni of protecting the oxidative damage of vascular endothelial cells cultured in high glucose medium[J].Chin Pharmacol Bull(中国药理学通报),2011,27:1077-1080.
[10]Chen Y,Liu Z,Zhou F,et al.Evaluating pharmacological effects of two major components of Shuangdan oral liquid:role of Danshensu and Paeonol in diabetic nephropathy rat[J].Biomol Ther(Seoul),2016,24:536-542.
[11]Sue J,Zhang BX,Yan SR,et al.Study on self-similarity of property combination mode of traditional Chinese medicines[J].Chin J Chin Mater Med(中国中药杂志),2014,39:2378-2381.
[12]Sue J,Zhang YL,Gu H,et al.Theoretical model for compatibility of medicinal property combination of traditional Chinese medicines[J].Chin J Chin Mater Med(中国中药杂志),2015,40:3327-3330.
[13]Kuang HX,Cheng W.Studies on the capability of properties and flavors of Chinese medicine to be split and combined-a new assumption on the theory of properties and flavors of Chinese medicine and the research method[J].World Sci Technol/Mod Tradit Chin Med Mater Med(世界科学技术:中医药现代化),2009,11:768-771.
[14]Kim KC,Kim JS,Son JK,et al.Enhanced induction of mitochondrial damage and apoptosis in human leukemia HL-60 cells by the Ganoderma lucidum and Duchesnea chrysantha extracts[J].Cancer Lett,2007,246:210-217.
[15]Li Y,Yang W,Zhu Q,et al.Protective effects on vascular endothelial cell in N'-nitro-L-arginine(L-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani[J].Biosci Trends,2015,9:237-244.
[16]Liu CX.Reflections on TCM modernization and TCM international development[J].China Pharm(中国药房),2016,27:1441-1444.
[17]Wang S,Hu Y,Tan W,et al.Compatibility art of traditional Chinese medicine:from the perspective of herb pairs[J].JEthnopharmacol,2012,143:412-423.
[18]Sheng HG.Progress on model of component formula in Chinese medicine[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2013,19:349-352.
[19]Rao CV,Rivenson A,Simi B,et al.Chemoprevention of colon carcinogenesis by dietary curcumin,a naturally occurring plant phenolic compound[J].Cancer Res,1995,55:259-266.
[20]Jing Y,Ohizumi H,Kawazoe N,et al.Selective inhibitory effect of bufalin on growth of human tumor cells in vitro:association with the induction of apoptosis in leukemia HL-60 cells[J].Jpn J Cancer Res,1994,85:645-651.
[21]Giri B,Gomes AR,Sengupta R,et al.Curcumin synergizes the growth inhibitory properties of Indian toad(Bufo melanostictus Schneider)skin-derived factor(BM-ANF1)in HCT-116 colon cancer cells[J].Anticancer Res,2009,29:395-401.
[22]Yu LY,Wang Y,Fan XH,et al.Discovering active components from traditional Chinese medicine by component-knockout approach[J].Chin J Chin Mater Med(中国中药杂志),2009,34:336-339.
[23]Yu LY.Study on Effective Substances in Shenmai Formula(参麦方药效物质基础研究)[D].Hangzhou:Zhejiang University,2010.
[24]Wu X,Liao HB,Zhu XH,et al.The screening of active component with antineoplastic activity in vitro from the seeds of Amorpha fruticosa[J].J Pharm Res(药学研究),2016,35:449-452.
[25]Zhou Y,Ren Y,Ma Z,et al.Identification and quantification of the major volatile constituents in antidepressant active fraction of Xiaoyaosan by gas chromatography-mass spectrometry[J].JEthnopharmacol,2012,141:187-192.
[26]Ren YL,Jia GC,Ma ZJ,et al.Screening of antidepressant fractions from Xiaoyaosan[J].Lishizhen Med Mater Med Res(时珍国医国药),2012,23:2689-2691.
[27]Duan Y,Pei K,Cai H,et al.Bioactivity evaluation-based ultra high-performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry and novel distinction of multi-subchemome compatibility recognition strategy with Astragali Radix-Fructus Corni herb-pair as a case study[J].J Pharm Biomed Anal,2016,129:514-534.
[28]Du GH.Modern drug screening techniques approach on CMM[J].World Sci Technol-Mod Tradit Chin Med(世界科学技术-中医药现代化),2000,2:47-52.
[29]Snowden M,Green DV.The impact of diversity-based,highthroughput screening on drug discovery:"chance favours the prepared mind"[J].Curr Opin Drug Discov Dev,2008,11:553-558.
[30]Hu YX,Cai W,Zhang HH,et al.Establishment and application of screening model for anti-asthmatic natural active ingredients based on bitter taste receptors in lung tissue[J].Chin Tradit Herb Drugs(中草药),2016,47:775-780.
[31]Hao HP,Zheng CN,Wang GJ.Thoughts and experimental exploration on pharmacokinetic study of herbal medicines with multiple-components and targets[J].Acta Pharm Sin(药学学报),2009,44:270-275.
[32]Qiao X,Wang Q,Wang S,et al.A 42-markers pharmacokinetic study reveals interactions of berberine and glycyrrhizic acid in the anti-diabetic Chinese medicine formula Gegen-Qinlian decoction[J].Front Pharmacol,2018,9:622.
[33]Liu ZH,Sun XB.Network pharmacology:new opportunity for the modernization of traditional Chinese medicine[J].Acta Pharm Sin(药学学报),2012,47:696-703.
[34]Liu AL,Du GH.Network pharmacology:new guidelines for drug discovery[J].Acta Pharm Sin(药学学报),2010,45:1472-1477.
[35]Yue SJ,Xin LT,Fan YC,et al.Herb pair Danggui-Honghua:mechanisms underlying blood stasis syndrome by system pharmacology approach[J].Sci Rep,2017,7:40318.
[36]Zhao L,Wu YF,Gao Y,et al.Intervention mechanism of psychological sub-health by Baihe Dihuang Tang based on network pharmacology[J].Acta Pharm Sin(药学学报),2017,52:99-105.
[37]Hao J,Jin Z,Zhu H,et al.Antiestrogenic activity of the Xi-Huang formula for breast cancer by targeting the estrogen receptorα[J].Cell Physiol Biochem,2018,47:2199-2215.
[38]Zhai JP,Deng YY,Lang AD.Research advances in application of biochip in traditional Chinese medicine[J].Pharm Biotechnol(药物生物技术),2013,20:72-75.
[39]Ren G,Fan X,Liang Q,et al.Screening and evaluation of traditional Chinese medicine by microarray expression analysis[J].JEthnopharmacol,2013,147:564-569.
[40]Zhou SW,Qiu RQ,Gao CF,et al.Application of biochromatography in screening the bioactive components from traditional Chinese medicine[J].Chin J Pharm(中国医药工业杂志),2017,48:1692-1697.
[41]Yang YX,Li SY,Zhang Q,et al.Applications of immobilization techniques in the screening of active constituents from traditional Chinese medicines[J].Acta Pharm Sin(药学学报),2017,52:198-205.
[42]Xue H,Cheng Y,Wang X,et al.Rutaecarpine and evodiamine selected asβ1-AR inhibitor candidates usingβ1-AR/CMC-offlineUPLC/MS prevent cardiac ischemia-reperfusion injury via energy modulation[J].J Pharm Biomed Anal,2015,115:307-314.
[43]Tong XL,Wu YC,Ji HY,et al.Reestablishement of herbal dosage for classic formulae[J].Shanghai J Tradit Chin Med(上海中医药杂志),2009,43:1-4.
[44]Pang HQ,Tang YP,Shi XQ,et al.Comparison on promoting blood effects of different effective parts of Salvia miltiorrhiza by principal component analysis and multi-attribute comprehensive index methods[J].Chin Tradit Herb Drugs(中草药),2016,47:3239-3247.
[45]Liu TZ,Wang S,Li TJ,et al.Study on the efficacy components compatibility optimization of Arisaema amurense Maxim treating lung cancer based on uniform design[J].Lishizhen Med Mater Med Res(时珍国医国药),2017,29:237-239.
[46]Miao JX,Miao MS.Research status,problems and thinking of multi-component of Chinese medicine[J].China J Chin Med(中医学报),2012,27:1116-1119.
[47]Zhou M,Hong YL,Lin X,et al.Recent pharmaceutical evidence on the compatibility rationality of traditional Chinese medicine[J].J Ethnopharmacol,2017,206:363-375.
[48]Zhu GQ,Teng HY,Huang P.Application of orthogonal experimental design method in medicine[J].J Math Med(数理医药学杂志),2007,20:568-569.
[49]Li F,Fan XX,Chu C,et al.A strategy for optimizing the combination of active components based on Chinese medicinal formula Sheng-Mai-San for myocardial ischemia[J].Cell Physiol Biochem,2018,45:1455-1471.
[50]Zhang WH,Wang BH,Zhang XF,et al.Effect of compatibility of traditional Chinese medicine on the content of amygdalin in"Ma-Xing-Yi-Gan-Tang"[J].Chin J Tradit Med Sci Technol(中国中医药科技),2014,21:406-407.
[51]Xu WJ,Zhou HH,Chen SD.Uniform design research on complex prescription of Chinese medicine[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2010,16:236-239.
[52]Wu Y,Pan X,Xu Y,et al.Optimization of combinations of ginsenoside-Rg1,ginsenoside-Rb1,evodiamine and rutaecarpine for effective therapy of mouse migraine[J].J Nat Med,2016,70:207-216.
[53]Tong X,Chen GF,Lu Y,et al.Uniform designed research on the active ingredients assembling of huangqi decoction for inhibition of DMN-induced liver fibrosis[J].Chin J Integr Tradit West Med(中国中西医结合杂志),2011,31:1389-1393.
[54]Shang HC,Zhang BL,Wang YY,et al.A method for proportion screening of TCM small prescriptions[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2003,9:1-3.
[55]Sheng YM,Chen L,Xie XL.Optimization of the best ratio of Astragalus polysaccharide and Astragalus saponin for immunomodulation based on increase-decrease baseline geometric proportion design method[J].Chin J Hosp Pharm(中国医院药学杂志),2016,36:59-62.
[56]Fan BB,Zhao XM,Guo YH,et al.Optimization of the best ratio of Radix aucklandiae active monomer against diarrhea based on increase-decrease baseline geometric proportion design method[J].Guide Chin Med(中国医药指南),2017,15:14-15.
[57]Tang CH,Cai SX.Application of the combined use of uniform experimental design and orthogonal experimental design in biomedicial engineering[J].J Biomed Eng(生物医学工程学杂志),2006,23:1228-1231.
[58]Tang CH,Cai SX,Wang BC.Optimization of the best ratio of Ginkgo formulae based on combining uniform design with orthogonal design[J].Chin Tradit Herb Drugs(中草药),2005,36:63-64.
[59]Sun WM,Sun RY.The orthogonal T value method for the study of traditional Chinese medicine prescriptions[J].Pharmacol Clin Chin Mater Med(中药药理与临床),1992,8:41-45.
[60]Lu ZZ,Li ZB,Ma X,et al.Compound optimization of effective components of traditional Chinese medicine in treatment of acute gouty arthritis by orthogonal T value method[J].Med Pharm J Chin People's Liberation Army(解放军医药杂志),2016,28:33-41.
[61]Yang Y,Zhang G,Sun Q,et al.Simultaneous determination of 8 compounds in Gancao-Ganjiang-Tang by HPLC-DAD and analysis of the relations between compatibility,dosage,and contents of medicines[J].Evid Based Complement Alternat Med,2017,2017:4703632.
[62]Shi L,Tang X,Dang X,et al.Investigating herb-herb interactions:the potential attenuated toxicity mechanism of the combined use of Glycyrrhizae radix et rhizoma(Gancao)and Sophorae flavescentis radix(Kushen)[J].J Ethnopharmacol,2015,165:243-250.
[63]Wang R,Wang YY,Cheng ZH,et al.Current situation and prospects of optimization mathematic method for dose proportion of complex prescription[J].J Beijing Univ Tradit Chin Med(北京中医药大学学报),2005,28:8-11.
[64]Huang LP,Wang TM,Yang HY,et al.Application of uniform design to screen active ingredient and its proportion of maxing Shigan decoction[J].Liaoning J Tradit Chin Med(辽宁中医杂志),2015,42:151-153.
[65]Du GH,Wang YH,Zhang R,et al.Multi-target and multicomponent pattern,a superficial understanding of the action mechanism of traditional Chinese medicine[J].World Sci Technol-Mod Tradit Chin Med(世界科学技术-中医药现代化),2009,11:480-484.
[66]Duan Y,Pei K,Cai H,et al.Strategy of integrated evaluation on treatment of traditional Chinese medicine as'interaction of system to system'and establishment of novel fuzzy target contribution recognition with herb-pairs,a case study on Astragali Radix-Fructus Corni[J].Mol Cell Endocrinol,2016,434:219-237.
[67]Ma RJ,Miao MS.A new method for evaluating the efficacy of Chinese medicine:the comprehensive weight coefficient method[J].Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理),2011,22:569-572.
[68]Yang H,Wu Y,Ma YY,et al.Study on compatibility of traditional Chinese medicine active constituents with antioxidant activity[J].China J Chin Mater Med(中国中药杂志),2012,37:1826-1829.
[69]Wang R,Wang YY,Zhu JP,et al.ED-NM-MO trigeminy method for multi-objective optimization of compatibility for traditional Chinese medicine[J].Chin J Basic Med Tradit Chin Med(中国中医基础医学杂志),2005,11:887-889.
[70]Wang R,Shang HC,Wang YY,et al.Multi-objective optimization research of the ratio of Danshen and Sanchi with ED-NM-MOtrigeminy method[J].Tianjin J Tradit Chin Med(天津中医药),2006,23:242-247.
[2]Liu LM,Chen X,Yue GX,et al.Some thoughts on definition of"Chinese materia medica components"[J].Chin Tradit Herb Drugs(中草药),2018,49:2489-2495.
[3]Shang HC,Zhang BL,Gao XM.Study on compatibility of prescription[J].Chin J Inf Tradit Chin Med(中国中医药信息杂志),2002,9:6-9.
[4]Zhao HY,Zhang CL,Liu XY,et al.Study on the proportion of active ingredients in drug combination of"Suang-Huang-TongGuang-Xin-Pian"[J].Lishizhen Med Mater Med Res(时珍国医国药),2018,29:45-48.
[5]Zhou MM,Hong YL,Lin X,et al.Recent pharmaceutical evidence on the compatibility rationality of traditional Chinese medicine[J].J Ethnopharmacol,2017,206:363-375.
[6]Zhang Y,Wu XD,Yang Y.Role of traditional compatibility and components compatibility under the background of precision medicine[J].Acta Chin Med Pharmacol(中医药学报),2018,46:7-10.
[7]Duan Y,Pei K,Cai H,et al.A new strategy for exploring herb-pair investigation with a case study on Astragali Radix-Corni Fructus in treatment of diabetic nephropathy[J].China J Chin Mater Med(中国中药杂志),2016,41:3919-3926.
[8]Du H,Wang K,Su L,et al.Metabonomic identification of the effects of the Zhimu-Baihe saponins on a chronic unpredictable mild stress-induced rat model of depression[J].J Pharm Biomed Anal,2016,128:469-479.
[9]Xu HQ,Nong WH,Liu CD,et al.The effect of the best compatibility of components in Fruetus Corni of protecting the oxidative damage of vascular endothelial cells cultured in high glucose medium[J].Chin Pharmacol Bull(中国药理学通报),2011,27:1077-1080.
[10]Chen Y,Liu Z,Zhou F,et al.Evaluating pharmacological effects of two major components of Shuangdan oral liquid:role of Danshensu and Paeonol in diabetic nephropathy rat[J].Biomol Ther(Seoul),2016,24:536-542.
[11]Sue J,Zhang BX,Yan SR,et al.Study on self-similarity of property combination mode of traditional Chinese medicines[J].Chin J Chin Mater Med(中国中药杂志),2014,39:2378-2381.
[12]Sue J,Zhang YL,Gu H,et al.Theoretical model for compatibility of medicinal property combination of traditional Chinese medicines[J].Chin J Chin Mater Med(中国中药杂志),2015,40:3327-3330.
[13]Kuang HX,Cheng W.Studies on the capability of properties and flavors of Chinese medicine to be split and combined-a new assumption on the theory of properties and flavors of Chinese medicine and the research method[J].World Sci Technol/Mod Tradit Chin Med Mater Med(世界科学技术:中医药现代化),2009,11:768-771.
[14]Kim KC,Kim JS,Son JK,et al.Enhanced induction of mitochondrial damage and apoptosis in human leukemia HL-60 cells by the Ganoderma lucidum and Duchesnea chrysantha extracts[J].Cancer Lett,2007,246:210-217.
[15]Li Y,Yang W,Zhu Q,et al.Protective effects on vascular endothelial cell in N'-nitro-L-arginine(L-NNA)-induced hypertensive rats from the combination of effective components of Uncaria rhynchophylla and Semen Raphani[J].Biosci Trends,2015,9:237-244.
[16]Liu CX.Reflections on TCM modernization and TCM international development[J].China Pharm(中国药房),2016,27:1441-1444.
[17]Wang S,Hu Y,Tan W,et al.Compatibility art of traditional Chinese medicine:from the perspective of herb pairs[J].JEthnopharmacol,2012,143:412-423.
[18]Sheng HG.Progress on model of component formula in Chinese medicine[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2013,19:349-352.
[19]Rao CV,Rivenson A,Simi B,et al.Chemoprevention of colon carcinogenesis by dietary curcumin,a naturally occurring plant phenolic compound[J].Cancer Res,1995,55:259-266.
[20]Jing Y,Ohizumi H,Kawazoe N,et al.Selective inhibitory effect of bufalin on growth of human tumor cells in vitro:association with the induction of apoptosis in leukemia HL-60 cells[J].Jpn J Cancer Res,1994,85:645-651.
[21]Giri B,Gomes AR,Sengupta R,et al.Curcumin synergizes the growth inhibitory properties of Indian toad(Bufo melanostictus Schneider)skin-derived factor(BM-ANF1)in HCT-116 colon cancer cells[J].Anticancer Res,2009,29:395-401.
[22]Yu LY,Wang Y,Fan XH,et al.Discovering active components from traditional Chinese medicine by component-knockout approach[J].Chin J Chin Mater Med(中国中药杂志),2009,34:336-339.
[23]Yu LY.Study on Effective Substances in Shenmai Formula(参麦方药效物质基础研究)[D].Hangzhou:Zhejiang University,2010.
[24]Wu X,Liao HB,Zhu XH,et al.The screening of active component with antineoplastic activity in vitro from the seeds of Amorpha fruticosa[J].J Pharm Res(药学研究),2016,35:449-452.
[25]Zhou Y,Ren Y,Ma Z,et al.Identification and quantification of the major volatile constituents in antidepressant active fraction of Xiaoyaosan by gas chromatography-mass spectrometry[J].JEthnopharmacol,2012,141:187-192.
[26]Ren YL,Jia GC,Ma ZJ,et al.Screening of antidepressant fractions from Xiaoyaosan[J].Lishizhen Med Mater Med Res(时珍国医国药),2012,23:2689-2691.
[27]Duan Y,Pei K,Cai H,et al.Bioactivity evaluation-based ultra high-performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry and novel distinction of multi-subchemome compatibility recognition strategy with Astragali Radix-Fructus Corni herb-pair as a case study[J].J Pharm Biomed Anal,2016,129:514-534.
[28]Du GH.Modern drug screening techniques approach on CMM[J].World Sci Technol-Mod Tradit Chin Med(世界科学技术-中医药现代化),2000,2:47-52.
[29]Snowden M,Green DV.The impact of diversity-based,highthroughput screening on drug discovery:"chance favours the prepared mind"[J].Curr Opin Drug Discov Dev,2008,11:553-558.
[30]Hu YX,Cai W,Zhang HH,et al.Establishment and application of screening model for anti-asthmatic natural active ingredients based on bitter taste receptors in lung tissue[J].Chin Tradit Herb Drugs(中草药),2016,47:775-780.
[31]Hao HP,Zheng CN,Wang GJ.Thoughts and experimental exploration on pharmacokinetic study of herbal medicines with multiple-components and targets[J].Acta Pharm Sin(药学学报),2009,44:270-275.
[32]Qiao X,Wang Q,Wang S,et al.A 42-markers pharmacokinetic study reveals interactions of berberine and glycyrrhizic acid in the anti-diabetic Chinese medicine formula Gegen-Qinlian decoction[J].Front Pharmacol,2018,9:622.
[33]Liu ZH,Sun XB.Network pharmacology:new opportunity for the modernization of traditional Chinese medicine[J].Acta Pharm Sin(药学学报),2012,47:696-703.
[34]Liu AL,Du GH.Network pharmacology:new guidelines for drug discovery[J].Acta Pharm Sin(药学学报),2010,45:1472-1477.
[35]Yue SJ,Xin LT,Fan YC,et al.Herb pair Danggui-Honghua:mechanisms underlying blood stasis syndrome by system pharmacology approach[J].Sci Rep,2017,7:40318.
[36]Zhao L,Wu YF,Gao Y,et al.Intervention mechanism of psychological sub-health by Baihe Dihuang Tang based on network pharmacology[J].Acta Pharm Sin(药学学报),2017,52:99-105.
[37]Hao J,Jin Z,Zhu H,et al.Antiestrogenic activity of the Xi-Huang formula for breast cancer by targeting the estrogen receptorα[J].Cell Physiol Biochem,2018,47:2199-2215.
[38]Zhai JP,Deng YY,Lang AD.Research advances in application of biochip in traditional Chinese medicine[J].Pharm Biotechnol(药物生物技术),2013,20:72-75.
[39]Ren G,Fan X,Liang Q,et al.Screening and evaluation of traditional Chinese medicine by microarray expression analysis[J].JEthnopharmacol,2013,147:564-569.
[40]Zhou SW,Qiu RQ,Gao CF,et al.Application of biochromatography in screening the bioactive components from traditional Chinese medicine[J].Chin J Pharm(中国医药工业杂志),2017,48:1692-1697.
[41]Yang YX,Li SY,Zhang Q,et al.Applications of immobilization techniques in the screening of active constituents from traditional Chinese medicines[J].Acta Pharm Sin(药学学报),2017,52:198-205.
[42]Xue H,Cheng Y,Wang X,et al.Rutaecarpine and evodiamine selected asβ1-AR inhibitor candidates usingβ1-AR/CMC-offlineUPLC/MS prevent cardiac ischemia-reperfusion injury via energy modulation[J].J Pharm Biomed Anal,2015,115:307-314.
[43]Tong XL,Wu YC,Ji HY,et al.Reestablishement of herbal dosage for classic formulae[J].Shanghai J Tradit Chin Med(上海中医药杂志),2009,43:1-4.
[44]Pang HQ,Tang YP,Shi XQ,et al.Comparison on promoting blood effects of different effective parts of Salvia miltiorrhiza by principal component analysis and multi-attribute comprehensive index methods[J].Chin Tradit Herb Drugs(中草药),2016,47:3239-3247.
[45]Liu TZ,Wang S,Li TJ,et al.Study on the efficacy components compatibility optimization of Arisaema amurense Maxim treating lung cancer based on uniform design[J].Lishizhen Med Mater Med Res(时珍国医国药),2017,29:237-239.
[46]Miao JX,Miao MS.Research status,problems and thinking of multi-component of Chinese medicine[J].China J Chin Med(中医学报),2012,27:1116-1119.
[47]Zhou M,Hong YL,Lin X,et al.Recent pharmaceutical evidence on the compatibility rationality of traditional Chinese medicine[J].J Ethnopharmacol,2017,206:363-375.
[48]Zhu GQ,Teng HY,Huang P.Application of orthogonal experimental design method in medicine[J].J Math Med(数理医药学杂志),2007,20:568-569.
[49]Li F,Fan XX,Chu C,et al.A strategy for optimizing the combination of active components based on Chinese medicinal formula Sheng-Mai-San for myocardial ischemia[J].Cell Physiol Biochem,2018,45:1455-1471.
[50]Zhang WH,Wang BH,Zhang XF,et al.Effect of compatibility of traditional Chinese medicine on the content of amygdalin in"Ma-Xing-Yi-Gan-Tang"[J].Chin J Tradit Med Sci Technol(中国中医药科技),2014,21:406-407.
[51]Xu WJ,Zhou HH,Chen SD.Uniform design research on complex prescription of Chinese medicine[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2010,16:236-239.
[52]Wu Y,Pan X,Xu Y,et al.Optimization of combinations of ginsenoside-Rg1,ginsenoside-Rb1,evodiamine and rutaecarpine for effective therapy of mouse migraine[J].J Nat Med,2016,70:207-216.
[53]Tong X,Chen GF,Lu Y,et al.Uniform designed research on the active ingredients assembling of huangqi decoction for inhibition of DMN-induced liver fibrosis[J].Chin J Integr Tradit West Med(中国中西医结合杂志),2011,31:1389-1393.
[54]Shang HC,Zhang BL,Wang YY,et al.A method for proportion screening of TCM small prescriptions[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2003,9:1-3.
[55]Sheng YM,Chen L,Xie XL.Optimization of the best ratio of Astragalus polysaccharide and Astragalus saponin for immunomodulation based on increase-decrease baseline geometric proportion design method[J].Chin J Hosp Pharm(中国医院药学杂志),2016,36:59-62.
[56]Fan BB,Zhao XM,Guo YH,et al.Optimization of the best ratio of Radix aucklandiae active monomer against diarrhea based on increase-decrease baseline geometric proportion design method[J].Guide Chin Med(中国医药指南),2017,15:14-15.
[57]Tang CH,Cai SX.Application of the combined use of uniform experimental design and orthogonal experimental design in biomedicial engineering[J].J Biomed Eng(生物医学工程学杂志),2006,23:1228-1231.
[58]Tang CH,Cai SX,Wang BC.Optimization of the best ratio of Ginkgo formulae based on combining uniform design with orthogonal design[J].Chin Tradit Herb Drugs(中草药),2005,36:63-64.
[59]Sun WM,Sun RY.The orthogonal T value method for the study of traditional Chinese medicine prescriptions[J].Pharmacol Clin Chin Mater Med(中药药理与临床),1992,8:41-45.
[60]Lu ZZ,Li ZB,Ma X,et al.Compound optimization of effective components of traditional Chinese medicine in treatment of acute gouty arthritis by orthogonal T value method[J].Med Pharm J Chin People's Liberation Army(解放军医药杂志),2016,28:33-41.
[61]Yang Y,Zhang G,Sun Q,et al.Simultaneous determination of 8 compounds in Gancao-Ganjiang-Tang by HPLC-DAD and analysis of the relations between compatibility,dosage,and contents of medicines[J].Evid Based Complement Alternat Med,2017,2017:4703632.
[62]Shi L,Tang X,Dang X,et al.Investigating herb-herb interactions:the potential attenuated toxicity mechanism of the combined use of Glycyrrhizae radix et rhizoma(Gancao)and Sophorae flavescentis radix(Kushen)[J].J Ethnopharmacol,2015,165:243-250.
[63]Wang R,Wang YY,Cheng ZH,et al.Current situation and prospects of optimization mathematic method for dose proportion of complex prescription[J].J Beijing Univ Tradit Chin Med(北京中医药大学学报),2005,28:8-11.
[64]Huang LP,Wang TM,Yang HY,et al.Application of uniform design to screen active ingredient and its proportion of maxing Shigan decoction[J].Liaoning J Tradit Chin Med(辽宁中医杂志),2015,42:151-153.
[65]Du GH,Wang YH,Zhang R,et al.Multi-target and multicomponent pattern,a superficial understanding of the action mechanism of traditional Chinese medicine[J].World Sci Technol-Mod Tradit Chin Med(世界科学技术-中医药现代化),2009,11:480-484.
[66]Duan Y,Pei K,Cai H,et al.Strategy of integrated evaluation on treatment of traditional Chinese medicine as'interaction of system to system'and establishment of novel fuzzy target contribution recognition with herb-pairs,a case study on Astragali Radix-Fructus Corni[J].Mol Cell Endocrinol,2016,434:219-237.
[67]Ma RJ,Miao MS.A new method for evaluating the efficacy of Chinese medicine:the comprehensive weight coefficient method[J].Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理),2011,22:569-572.
[68]Yang H,Wu Y,Ma YY,et al.Study on compatibility of traditional Chinese medicine active constituents with antioxidant activity[J].China J Chin Mater Med(中国中药杂志),2012,37:1826-1829.
[69]Wang R,Wang YY,Zhu JP,et al.ED-NM-MO trigeminy method for multi-objective optimization of compatibility for traditional Chinese medicine[J].Chin J Basic Med Tradit Chin Med(中国中医基础医学杂志),2005,11:887-889.
[70]Wang R,Shang HC,Wang YY,et al.Multi-objective optimization research of the ratio of Danshen and Sanchi with ED-NM-MOtrigeminy method[J].Tianjin J Tradit Chin Med(天津中医药),2006,23:242-247.