从血管内皮功能调控探讨中医外治法的活血化瘀效应
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摘要
针灸、推拿、拔罐属于常见中医外治法,其本质上均属于机械力刺激。中医外治法临床取效的关键途径之一就是发挥其活血化瘀效应,从细胞生物学原理看就是通过外治法的机械力刺激对血管内皮功能调控,引发细胞生物学效应。但既往中医外治法的活血化瘀机制的研究多集中在宏观层面的研究,缺少细胞分子等微观层面的探讨,给临床、教学、科研带来诸多不便,严重制约中医外治法发展。本文就常见中医外治法活血化瘀理论溯源、符合中医外治法特点的相关细胞学模型的构建、中医外治调控血管内皮功能发挥活血化瘀效应等方面来研究中医外治法调控血管内皮功能的细胞微观学机制,进一步研究其活血化瘀效应的作用机制。
Acupuncture,massage,and cupping are common Chinese medicine external treatment methods,which are essentially mechanical stimulation. One of the key ways of clinical treatment of TCM external treatment is to exert its effect of promoting blood circulation and removing blood stasis. From the perspective of cell biology,it is the regulation of vascular endothelial function by mechanical stimulation of external treatment,which triggers cell biological effects. However,the research on the mechanism of promoting blood circulation and removing blood stasis in the Chinese medicine treatment has focused on macroscopic research,lacking microscopic discussion of cell molecules,and bringing inconvenience to clinical,teaching and scientific research,seriously restricting the development of TCM treatment. In this paper,we study the microcosmic mechanism of TCM external therapy in regulating vascular endothelial function from the aspects of tracing the theory of promoting blood circulation and removing blood stasis,constructing relevant cytological models that accord with the characteristics of TCM external therapy,and exerting the effect of activating blood circulation and removing blood stasis,so as to further study the mechanism of its effect of activating blood circulation and removing blood stasis.
Acupuncture,massage,and cupping are common Chinese medicine external treatment methods,which are essentially mechanical stimulation. One of the key ways of clinical treatment of TCM external treatment is to exert its effect of promoting blood circulation and removing blood stasis. From the perspective of cell biology,it is the regulation of vascular endothelial function by mechanical stimulation of external treatment,which triggers cell biological effects. However,the research on the mechanism of promoting blood circulation and removing blood stasis in the Chinese medicine treatment has focused on macroscopic research,lacking microscopic discussion of cell molecules,and bringing inconvenience to clinical,teaching and scientific research,seriously restricting the development of TCM treatment. In this paper,we study the microcosmic mechanism of TCM external therapy in regulating vascular endothelial function from the aspects of tracing the theory of promoting blood circulation and removing blood stasis,constructing relevant cytological models that accord with the characteristics of TCM external therapy,and exerting the effect of activating blood circulation and removing blood stasis,so as to further study the mechanism of its effect of activating blood circulation and removing blood stasis.
引文
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[15]李程,江瑜,陈磊,等.动态力学刺激对血管内皮细胞血管舒缩活性物质ET-1和PGI-2合成释放影响的研究[J].时珍国医国药,2014,1(1):封2.
[16]谢西梅,陈波,崔瑾,等.体外模拟动态力学刺激对SD 幼鼠前脂肪细胞生长活力影响的研究[J].辽宁中医杂志,2011,38(7):1453-1455.
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[18]杨志虹,肖金良,崔瑾,等.体外温热刺激对脐静脉血管内皮细胞线粒体及三磷酸腺苷含量影响的实验研究[J].贵阳中医学院学报,2014,36(2):1-4
[19]崔帅,崔瑾.中医罐法负压对人脐静脉血管内皮细胞迁移与分泌血栓调节蛋白(TM) 功能的影响[J].中医药临床杂志,2013,25(4):328-330.
[20]眭承志,刘志坤,陈少玫,等.绝经后骨质疏松症血瘀病机的微观分子生物学论证[J].中医研究,2005,18(4):19-21.
[21]马惠昇,张宏,严隽陶,等.推拿法样刺激对人脐静脉内皮细胞内钙离子浓度的影响[J].中国组织工程研究与临床康复,2010,14(37):6887-6890.
[22]傅品来,范志勇,张竞之,等.模拟手法压力刺激对颈性眩晕血瘀证患者血清损伤后内皮细胞的影响[J].中国中医急症,2016,25(9):1656-1659.
[2]张竞之,刘吉昌,范志勇,等.从丹皮酚对TLR-NF-κB信号通路的影响探讨高血压病血瘀证的形成机制[J].广州中医药大学学报,2014,31(3):330-333.
[3]翟衍庆.针刺的活血化淤作用研究[J].甘肃中医,1994,7(4):13-14.
[4]蔡国伟,贾玉英,戴春林.针刺膈俞穴的活血化瘀作用[J].中国临床康复,2006,10(11):160.
[5]范志勇,傅品来,张竞之,等.机械力刺激对血管内皮细胞的应力效应及对推拿活血化瘀机制的启示[J].时珍国医国药,2016,27(10):2550-2552.
[6]马惠昇.推拿法行气活血效应机制浅析[J].江苏中医药,2007,39(3):39-41.
[7]浏丹,许世雄,成伟华,等.法推拿形成运动狭窄黏弹性血管血流动力学[J].复旦学报(自然科学版),2005,44(2):246.
[8]谭琳蓥,刘慧荣,吴焕淦,等.艾灸疗法活血化瘀作用研究进展[J].中华中医药学刊,2009,27(2):272-274.
[9]唐照亮,宋小鸽,王宁新,等.艾灸活血化瘀作用机制的研究[J].安徽中医学院学报,2004,2(2):24-27.
[10]田宇瑛,秦丽娜,张维波.不同拔罐负压对皮肤血流量影响的初步观察[J].针刺研究,2007,32(3):184-185.
[11]洪寿海,吴菲,卢轩,等.拔罐疗法作用机制探讨[J].中国针灸,2011,31(10):932-934.
[12]崔帅,崔瑾.拔罐疗法的负压效应机制研究进展[J].针刺研究,2012,37(6):502-503.
[13]张莉.拔罐疗法对皮肤局部温度和血红蛋白影响的研究[D].北京:北京中医药大学,2000.
[14]李超群,孟向文,郭义,等.健康成人大椎穴拔罐前后督脉穴表温度变化观察[C].北京:中国针灸学会第九届全国中青年针灸推拿学术研讨会论文集,2010:92-94.
[15]李程,江瑜,陈磊,等.动态力学刺激对血管内皮细胞血管舒缩活性物质ET-1和PGI-2合成释放影响的研究[J].时珍国医国药,2014,1(1):封2.
[16]谢西梅,陈波,崔瑾,等.体外模拟动态力学刺激对SD 幼鼠前脂肪细胞生长活力影响的研究[J].辽宁中医杂志,2011,38(7):1453-1455.
[17]马惠异,张宏,严隽陶,等.适于推拿手法细胞力学加载装置模型的设计与构建[J].中国组织工程研究与临床康复,2010,14(26):4751-4753.
[18]杨志虹,肖金良,崔瑾,等.体外温热刺激对脐静脉血管内皮细胞线粒体及三磷酸腺苷含量影响的实验研究[J].贵阳中医学院学报,2014,36(2):1-4
[19]崔帅,崔瑾.中医罐法负压对人脐静脉血管内皮细胞迁移与分泌血栓调节蛋白(TM) 功能的影响[J].中医药临床杂志,2013,25(4):328-330.
[20]眭承志,刘志坤,陈少玫,等.绝经后骨质疏松症血瘀病机的微观分子生物学论证[J].中医研究,2005,18(4):19-21.
[21]马惠昇,张宏,严隽陶,等.推拿法样刺激对人脐静脉内皮细胞内钙离子浓度的影响[J].中国组织工程研究与临床康复,2010,14(37):6887-6890.
[22]傅品来,范志勇,张竞之,等.模拟手法压力刺激对颈性眩晕血瘀证患者血清损伤后内皮细胞的影响[J].中国中医急症,2016,25(9):1656-1659.