猪传染性胸膜肺炎研究进展
摘要
猪传染性胸膜肺炎是由猪胸膜肺炎放线杆菌(APP)感染引起的以急性出血和慢性纤维素性胸膜肺炎为特征的一种高度传染性疾病,可引起患猪大量死亡,为养猪业的三大呼吸道疾病之一。流行病学研究发现猪传染性胸膜肺炎多呈混合感染,且有一些非典型菌株流行。除毒素外,蛋白酶、尿酶、黏附素、APP的糖基化系统、双组分系统CpxA/CpxR等也参与致病的过程。另外, App的脂多糖(LPS)可使宿主肺部的细胞产生大量的致炎细胞因子,参与肺部炎症的形成。病原与呼吸道上皮细胞吸附的表面分子、新致病因子特别是引起细胞因子暴发的病原分子的鉴定及其诱导的免疫力,将为组合疫苗的研制提供基础。研究APP感染与宿主免疫状况的关系将为免疫调节剂的筛选提供依据。文章对近年来该病的流行情况、发病机制和疫苗研究进展进行了综述,旨在为该病的防治和未来的研究方向提供参考。
引文
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[4] PERRY M B,ANGEN ?,MACLEAN L L,et al.An atypical biotype Ⅰ Actinobacillus pleuropneumoniae serotype 13 is present in North America [J].Vet Microbiol,2012,156(3-4):403-412.
[5] 刘亚娟,聂福平,杨俊,等.猪传染性胸膜肺炎放线杆菌LAMP方法的建立 [J].中国兽医学报,2016,36(2):200-205.
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[14] 周靓,雷连成,谢芳,等.胸膜肺炎放线杆菌三聚体自转运黏附素功能区的表达及黏附活性 [J].中国兽医学报,2011,31(3):342-346.
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[19] LI H,LIU F,PENG W,et al.The CpxA/CpxR two-component system affects biofilm formation and virulence in Actinobacillus pleuropneumoniae [J].Front Cell Infect Mi,2018,8:72
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[21] 张艳,刘海隆,曹宗喜,等.猪传染性胸膜肺炎疫苗免疫仔猪后抗体消长规律的研究 [J].黑龙江畜牧兽医,2017(10下):136-138.
[22] 张艳,刘海隆,林哲敏,等.仔猪传染性胸膜肺炎母源抗体消长规律的研究[J].江苏农业科学,2018,46(5):156-158.
[23] 张飞,曹三杰,文心田,等.猪传染性胸膜肺炎疫苗的研究进展 [J].中国兽医学报,2015,35(11):1880-1886.
[24] SACRISTAN R D P,MICHIELS A,MARTENS M,et al.Efficacy of vaccination against Actinobacillus pleuropneumoniae in two Belgian farrow-to-finish pig herds with a history of chronic pleurisy [J].Vet Rec,2014,174(12):333-334.
[25] 胡佩佩,杨婷婷,黄复深,等.猪APP rOmpP2诱导小鼠抗攻击感染的免疫保护力研究[J].农业生物技术学报,2017,25(2):299-306.
[26] ADKINS I,HOLUBOVA J,KOSOVA M,et al.Bacteria and their toxins tamed for immunotherapy [J].Curr Pharm Biotechnol,2012,13(8):1446-1473.
[27] 王勇,刘思国,王春来,等.猪传染性胸膜肺炎重组亚单位疫苗保护效果的评价及其免疫机制的研究 [J].中国兽医科学,2006,36(12):955-960.
[28] SHAO M L,WANG Y,WANG C L,et al.Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice [J].Acta Vet Scand,2010,52(1):1-8.
[29] 张丽芳,罗薇.外膜蛋白的结构、功能及应用研究进展 [J].中国畜牧兽医,2014,41(10):55-61.
[30] 赵香汝,杨汉春.细菌外膜蛋白的研究现状 [J].中国兽医杂志,1997,23(12):41-42.
[31] 刘燕,庞安娜,韦强,等.兔多杀性巴氏杆菌外膜蛋白A(OmpA)重组蛋白单克隆抗体的制备及潜在应用 [J].农业生物技术学报,2012,20(6):676-681.
[32] 杨婷婷,黄复深,张雨龙,等.重组猪胸膜肺炎放线杆菌NLPI蛋白诱导小鼠抗攻击感染的免疫保护力研究[J].农业生物技术学报,2018,26(8):1392-1400.
[2] NEGRETE-ABASCAL E,REYES M E,GARCíA R M,et al.Flagella and motility in Actinobacillus pleuropneumoniae [J].J Bacteriol,2003,185(2):664-668.
[3] BOSSé J T,LI Y,ANGEN ?,et al.Multiplex PCR assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3,5 to 8,10,and 12 [J].J Clin Microbiol,2014,52(7):2380-2385.
[4] PERRY M B,ANGEN ?,MACLEAN L L,et al.An atypical biotype Ⅰ Actinobacillus pleuropneumoniae serotype 13 is present in North America [J].Vet Microbiol,2012,156(3-4):403-412.
[5] 刘亚娟,聂福平,杨俊,等.猪传染性胸膜肺炎放线杆菌LAMP方法的建立 [J].中国兽医学报,2016,36(2):200-205.
[6] ITO H,MATSUMOTO A.Isolation and genetic characterization of an Actinobacillus pleuropneumoniae serovar K12:O3 strain [J].J Vet Diagn Invest,2015,27(1):102-106.
[7] 葛兆宏,陆广富,陶洁,等.猪传染性胸膜肺炎放线杆菌变异株的分离与鉴定 [J].扬州大学学报(农业与科学版),2012,33(4):10-13.
[8] PéREZ MáRQUEZ V M,OCHOA J L,CRUZ C V,et al.Isolation of Actinobacillus pleuropneumoniae from layer hens showing clinical signs of infectious coryza [J].Avian Dis,2014,58(4):638-641.
[9] 王艳.猪瘟与猪传染性胸膜肺炎混合感染的诊治 [J].江西农业,2018(2):40.
[10] 许英民.仔猪白痢与传染性胸膜肺炎混合感染的诊治 [J].兽医导刊,2009(7):54-55.
[11] LI B,FANG J,ZUO Z,et al.Activation of porcine alveolar macrophages by Actinobacillus pleuropneumoniae lipopolysaccharide via the TLR4/NF-κB mediated pathway [J].Infect Immun,2018,36(3):e00642-17.
[12] WANG L,QIN W,ZHANG J,et al.Adh enhances Actinobacillus pleuropneumoniae pathogenicity by binding to OR5M11 and activating p38 which induces apoptosis of PAMs and IL-8 release [J].Sci Rep,2016,6:24058.
[13] 计群,雷连成,杨舒心,等.胸膜肺炎放线杆菌adh基因敲除株与5b野生株的培养特性及致病性比较 [J].中国畜牧兽医,2013,40(3):107-109.
[14] 周靓,雷连成,谢芳,等.胸膜肺炎放线杆菌三聚体自转运黏附素功能区的表达及黏附活性 [J].中国兽医学报,2011,31(3):342-346.
[15] ZHOU Y,LI L,CHEN Z,et al.Adhesion protein ApfA of Actinobacillus pleuropneumoniae is required for pathogenesis and is a potential target for vaccine development [J].Clin Vaccine Immunol,2013,20(2):287-294.
[16] XIAO L,ZHOU L,SUN C,et al.Apa is a trimeric autotransporter adhesin of Actinobacillus pleuropneumoniae responsible for autoagglutination and host cell adherence [J].J Basic Microbiol,2012,52(5):598-607.
[17] SARGEANT H R,MCDOWALL K J,MILLER H M,et al.Dietary zinc oxide affects the expression of genes associated with inflammation:Transcriptome analysis in pig lets challenged with ETEC K88 [J].Vet Immunol Immunopathol,2010,137(1/2):120-129.
[18] CHIERS K,WAELE T D,PASMANS F,et al.Virulence factors of Actinobacillus pleuropneumoniae involved in colonization,persistence and induction of lesions in its porcine host [J].Vet Res,2010,41(5):65-81.
[19] LI H,LIU F,PENG W,et al.The CpxA/CpxR two-component system affects biofilm formation and virulence in Actinobacillus pleuropneumoniae [J].Front Cell Infect Mi,2018,8:72
[20] 杜爱庆,刁有祥,张伟,等.猪胸膜肺炎放线杆菌溶血毒素的克隆表达及其对小鼠免疫保护作用 [J].微生物学报,2008,48(3):342-348.
[21] 张艳,刘海隆,曹宗喜,等.猪传染性胸膜肺炎疫苗免疫仔猪后抗体消长规律的研究 [J].黑龙江畜牧兽医,2017(10下):136-138.
[22] 张艳,刘海隆,林哲敏,等.仔猪传染性胸膜肺炎母源抗体消长规律的研究[J].江苏农业科学,2018,46(5):156-158.
[23] 张飞,曹三杰,文心田,等.猪传染性胸膜肺炎疫苗的研究进展 [J].中国兽医学报,2015,35(11):1880-1886.
[24] SACRISTAN R D P,MICHIELS A,MARTENS M,et al.Efficacy of vaccination against Actinobacillus pleuropneumoniae in two Belgian farrow-to-finish pig herds with a history of chronic pleurisy [J].Vet Rec,2014,174(12):333-334.
[25] 胡佩佩,杨婷婷,黄复深,等.猪APP rOmpP2诱导小鼠抗攻击感染的免疫保护力研究[J].农业生物技术学报,2017,25(2):299-306.
[26] ADKINS I,HOLUBOVA J,KOSOVA M,et al.Bacteria and their toxins tamed for immunotherapy [J].Curr Pharm Biotechnol,2012,13(8):1446-1473.
[27] 王勇,刘思国,王春来,等.猪传染性胸膜肺炎重组亚单位疫苗保护效果的评价及其免疫机制的研究 [J].中国兽医科学,2006,36(12):955-960.
[28] SHAO M L,WANG Y,WANG C L,et al.Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice [J].Acta Vet Scand,2010,52(1):1-8.
[29] 张丽芳,罗薇.外膜蛋白的结构、功能及应用研究进展 [J].中国畜牧兽医,2014,41(10):55-61.
[30] 赵香汝,杨汉春.细菌外膜蛋白的研究现状 [J].中国兽医杂志,1997,23(12):41-42.
[31] 刘燕,庞安娜,韦强,等.兔多杀性巴氏杆菌外膜蛋白A(OmpA)重组蛋白单克隆抗体的制备及潜在应用 [J].农业生物技术学报,2012,20(6):676-681.
[32] 杨婷婷,黄复深,张雨龙,等.重组猪胸膜肺炎放线杆菌NLPI蛋白诱导小鼠抗攻击感染的免疫保护力研究[J].农业生物技术学报,2018,26(8):1392-1400.