辛夷、辛夷仁和辛夷外苞片中挥发性成分的比较
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摘要
目的分析和比较辛夷全花蕾、辛夷仁和辛夷外苞片中挥发性成分的差异,为经典名方中以辛夷仁入药提供理论依据。方法采用水蒸气蒸馏提取挥发油,气相色谱质谱联用(GC-MS)技术测定辛夷和辛夷仁中的挥发性成分,采用谱库检索结合保留指数定性对挥发性成分进行鉴定,用峰面积归一化法计算各成分的相对含量,利用主成分分析(PCA)对数据进行统计处理。结果全花蕾、辛夷仁和辛夷外苞片中挥发油平均含量分别为1.70%、6.79%和0.45%,采用GC-MS共分别鉴定出87、82、85种化合物,主要为萜烯烃类和醇类化合物。以GC-MS数据结合PCA可以很好地区分辛夷的不同部位和不同产地。结论辛夷仁所含挥发性成分种类与辛夷一致,但含量远高于辛夷全花蕾,经典名方以辛夷仁入药具有科学性。
Objective To analyze the volatile components of Magnoliae Flos, Magnoliae Flos kernels, and Magnoliae Flos bracts and compare the differences of these components in order to provide a theoretical basis for the usage of kernels of Magnolia Flos in classical famous prescriptions. Methods The volatile components were extracted by steam distillation and analyzed by gas chromatography-mass spectrometry(GC-MS). The volatile components in different parts of Magnoliae Flos were identified by searching the retention index in the mass spectrometry database. The relative content of volatile components was calculated by peak area normalization method, and the data of GC-MS was further proceeded by principal component analysis(PCA). Results The average volatile components content in Magnoliae Flos, Magnoliae Flos kernels, and Magnoliae Flos bracts was 1.70%, 6.79%, and 0.45%, respectively. The numbers of compounds identified by GC-MS in those three different parts were 87, 82, and 85, respectively. These compounds mainly included terpene olefins and alcohols type chemicals. PCA method was then applied to process the GC-MS data and distinguish different parts and habitats of Magnoliae Flos. Conclusion The volatile components in the kernels of Magnoliae Flos were generally the same as those of Magnoliea Flos, while the content of which was much higher than those of whole flower bud of Magnoliae Flos. The results indicated that it was rational to use the kernels of Magnolia Flos in classical famous prescriptions.
Objective To analyze the volatile components of Magnoliae Flos, Magnoliae Flos kernels, and Magnoliae Flos bracts and compare the differences of these components in order to provide a theoretical basis for the usage of kernels of Magnolia Flos in classical famous prescriptions. Methods The volatile components were extracted by steam distillation and analyzed by gas chromatography-mass spectrometry(GC-MS). The volatile components in different parts of Magnoliae Flos were identified by searching the retention index in the mass spectrometry database. The relative content of volatile components was calculated by peak area normalization method, and the data of GC-MS was further proceeded by principal component analysis(PCA). Results The average volatile components content in Magnoliae Flos, Magnoliae Flos kernels, and Magnoliae Flos bracts was 1.70%, 6.79%, and 0.45%, respectively. The numbers of compounds identified by GC-MS in those three different parts were 87, 82, and 85, respectively. These compounds mainly included terpene olefins and alcohols type chemicals. PCA method was then applied to process the GC-MS data and distinguish different parts and habitats of Magnoliae Flos. Conclusion The volatile components in the kernels of Magnoliae Flos were generally the same as those of Magnoliea Flos, while the content of which was much higher than those of whole flower bud of Magnoliae Flos. The results indicated that it was rational to use the kernels of Magnolia Flos in classical famous prescriptions.
引文
[1]王永慧,叶方,张秀华.辛夷药理作用和临床应用研究进展[J].中国医药导报,2012,9(16):12-14.
[2]Shen Y,Li C G,Zhou S F,et al.Chemistry and bioactivity of Flos Magnoliae,a Chinese herb for rhinitis and sinusitis[J].Curr Med Chem,2008,15(16):1616-1627.
[3]Liang Z,Yang E Y.The therapeutic effects of irrigating therapy with magnolia injection on chronic maxillary sinusitis experimentally induced among rabbits[J].Chin JOtolaryngol Integr Tradit West Med,2005,13(1):6-10.
[4]Kim G C,Lee S G,Park B S,et al.Magnoliae Flos induces apoptosis of RBL-2H3 cells via mitochondria and caspase[J].Int Arch Allergy Immunol,2003,131(2):101-110.
[5]雷斅,尚志钧.雷公炮炙论[M].合肥:安徽科学技术出版社,1991.
[6]何廉臣.实验药物学[M].福州:福建科学技术出版社,2008.
[7]华佗.华佗神方[M].重庆:中外出版社,1979.
[8]王怀隐.太平圣惠方[M].北京:人民卫生出版社,1958.
[9]严用和.重订严氏济生方[M].北京:人民卫生出版社,1980.
[10]吴昆.医方考[M].北京:人民卫生出版社,2007.
[11]陶承熹.惠直堂经验方[M].北京:中医古籍出版社,1994.
[12]武之望,汪淇,张黎临,等.济阴纲目[M].北京:人民军医出版社,2009.
[13]龚廷贤,彭建中,程昭.寿世保元[M].北京:人民卫生出版社,2001.
[14]寇宗奭.本草衍义[M].北京:中国医药科技出版社,2012.
[15]张鑫,毛多斌.辛夷不同部位挥发油化学组份的对比研究[J].郑州轻工业学院学报,1999,14(3):24-26.
[16]Sook E,Choi K Y,Kim S C,et al.Pattern recognition of the herbal drug,Magnoliae Flos according to their essential oil components[J].Bull Kor Chem Soc,2009,30(5):1121-1126.
[17]廖圣良,商士斌,沈明贵,等.蒎烯及其衍生化合物药物活性的研究进展[J].化学试剂,2016,38(3):219-223.
[18]Sun Y,Cai X,Cao J,et al.Effects of 1,8-cineole on carbohydrate metabolism related cell structure changes of Salmonella[J].Front Microbiol,2018,9:1078.
[19]赵振东,苏文强,陈风雨,等.金合欢醇的资源及其生物活性应用研究进展[J].林产化学与工业,2005,25(S1):175-178.
[20]丁元刚,马红梅,张伯礼.樟脑药理毒理研究回顾及安全性研究展望[J].中国药物警戒,2012,9(1):38-42.
[2]Shen Y,Li C G,Zhou S F,et al.Chemistry and bioactivity of Flos Magnoliae,a Chinese herb for rhinitis and sinusitis[J].Curr Med Chem,2008,15(16):1616-1627.
[3]Liang Z,Yang E Y.The therapeutic effects of irrigating therapy with magnolia injection on chronic maxillary sinusitis experimentally induced among rabbits[J].Chin JOtolaryngol Integr Tradit West Med,2005,13(1):6-10.
[4]Kim G C,Lee S G,Park B S,et al.Magnoliae Flos induces apoptosis of RBL-2H3 cells via mitochondria and caspase[J].Int Arch Allergy Immunol,2003,131(2):101-110.
[5]雷斅,尚志钧.雷公炮炙论[M].合肥:安徽科学技术出版社,1991.
[6]何廉臣.实验药物学[M].福州:福建科学技术出版社,2008.
[7]华佗.华佗神方[M].重庆:中外出版社,1979.
[8]王怀隐.太平圣惠方[M].北京:人民卫生出版社,1958.
[9]严用和.重订严氏济生方[M].北京:人民卫生出版社,1980.
[10]吴昆.医方考[M].北京:人民卫生出版社,2007.
[11]陶承熹.惠直堂经验方[M].北京:中医古籍出版社,1994.
[12]武之望,汪淇,张黎临,等.济阴纲目[M].北京:人民军医出版社,2009.
[13]龚廷贤,彭建中,程昭.寿世保元[M].北京:人民卫生出版社,2001.
[14]寇宗奭.本草衍义[M].北京:中国医药科技出版社,2012.
[15]张鑫,毛多斌.辛夷不同部位挥发油化学组份的对比研究[J].郑州轻工业学院学报,1999,14(3):24-26.
[16]Sook E,Choi K Y,Kim S C,et al.Pattern recognition of the herbal drug,Magnoliae Flos according to their essential oil components[J].Bull Kor Chem Soc,2009,30(5):1121-1126.
[17]廖圣良,商士斌,沈明贵,等.蒎烯及其衍生化合物药物活性的研究进展[J].化学试剂,2016,38(3):219-223.
[18]Sun Y,Cai X,Cao J,et al.Effects of 1,8-cineole on carbohydrate metabolism related cell structure changes of Salmonella[J].Front Microbiol,2018,9:1078.
[19]赵振东,苏文强,陈风雨,等.金合欢醇的资源及其生物活性应用研究进展[J].林产化学与工业,2005,25(S1):175-178.
[20]丁元刚,马红梅,张伯礼.樟脑药理毒理研究回顾及安全性研究展望[J].中国药物警戒,2012,9(1):38-42.