苗药黑骨藤不同极性提取部位急性毒性及抗氧化研究
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摘要
[目的]对黑骨藤不同极性部位进行急性毒性试验及抗氧化研究,确定其临床用药安全性及活性部位。[方法]急性经口毒性试验采用SD(180～220 g)大鼠,连续灌胃给药7 d(9.75 g/kg)后,测定血清中的肝功能和肾功能等相关指标,观察其肝脏、肾脏毒性反应,采用ABTS(agreement of basic telecommunications services)法测定肝脏中的总抗氧化能力。[结果]黑骨藤不同极性部位给药7 d后,大鼠未出现死亡,石油醚部位、氯仿部位、醋酸乙酯部位、正丁醇部位组对肝脏和肾脏生化指标均无显著性影响(P>0.05),水部位组大鼠血清中的ALP和BUN显著升高(P<0.05)。[结论]黑骨藤的不同极性部位中的石油醚部位、氯仿部位、醋酸乙酯部位、正丁醇部位未出现毒性反应,水部位中的ALP和BUN升高,可能表现出微低毒性。氯仿部位和正丁醇部位表现出较强的抗氧化能力。
[Objective]To determine the safety of clinical medication and active fractions screening of Perploca forrestii Schltr.,we conducted acute toxicity experiment and antioxidative study of different polarity extractions from Perploca forrestii. [Methods]SD rats( 180-220 g) used to study acute oral toxicity test,was given different polarity extractions from Perploca forrestii. 9.75 g/kg by gavage for 7 days,to determinate the relevant indicators of liver function and renal function etc in serum,and to observe the toxicity of liver and kidney. ABTS( agreement of basic telecommunications services) used to determinate of total antioxidant capacity in the liver. [Result]The rats were given drugs by gavage for 7 days,and did not die,there was no significant effect on liver and kidney biochemical indexes in petroleum ether,chloroform,ethyl acetate and n-butanol group( P>0.05),the ALP and BUN were significantly increased in the serum of the rat of water group( P<0.05). [Conclusion]There was no toxic reaction in the petroleum ether,chloroform,ethyl acetate and n-butanol group,elevation of ALP and BUN in the water group may show microtoxicity. The oxidation resistance of chloroform and n-butanol group was strong.
[Objective]To determine the safety of clinical medication and active fractions screening of Perploca forrestii Schltr.,we conducted acute toxicity experiment and antioxidative study of different polarity extractions from Perploca forrestii. [Methods]SD rats( 180-220 g) used to study acute oral toxicity test,was given different polarity extractions from Perploca forrestii. 9.75 g/kg by gavage for 7 days,to determinate the relevant indicators of liver function and renal function etc in serum,and to observe the toxicity of liver and kidney. ABTS( agreement of basic telecommunications services) used to determinate of total antioxidant capacity in the liver. [Result]The rats were given drugs by gavage for 7 days,and did not die,there was no significant effect on liver and kidney biochemical indexes in petroleum ether,chloroform,ethyl acetate and n-butanol group( P>0.05),the ALP and BUN were significantly increased in the serum of the rat of water group( P<0.05). [Conclusion]There was no toxic reaction in the petroleum ether,chloroform,ethyl acetate and n-butanol group,elevation of ALP and BUN in the water group may show microtoxicity. The oxidation resistance of chloroform and n-butanol group was strong.
引文
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[13]CHEN L,LI J S,KE X,et al.The therapeutic effects of Periploca forrestii Schltr.Stem extracts on collagen-induced arthritis by inhibiting the activation of Src/NF-κB signaling pathway in rats[J]. J Ethnopharmacology,2017,202:12-19.
[14]李维熙,李怡芳,何蓉蓉.儿茶素类化合物抗氧化评价方法及作用机制的研究现状[J].中药新药与临床药理,2016,27(2):295-303.
[15]陈凡,马善丽,许颖,等.超高压处理对豆乳总多酚、类黄酮含量及其抗氧化性的影响[J].大豆科学,2011,30(2):310-313.
[2]SACHIDANANDAM K,FAGAN S C,ERGUL A.Oxidative stress and cardiovascular disease:Antioxidants and unresolved issues[J].Cardiovasc Drug Rev,2005,23(2):115-132.
[3]李洋,马文平,倪志婧.宁夏枸杞体外抗氧化机理研究[J].食品科学,2014,35(1):79-84.
[4]刘明,刘刚,刘育辰,等.黑骨藤镇痛抗炎作用活性部位筛选[J].药物评价研究,2014,37(4):322-325.
[5]张令令,肖智勇,马渊,等.苗药黑骨藤多糖部位HGT-5A的细胞免疫抑制作用及其可能的活性成分[J].中国药理学与毒理学杂志,2011,25(6):538-542.
[6]陈芳,汪毅.苗药黑骨藤的研究及开发应用[J].中国民族民间医药,2010,19(1):6-7.
[7]王秋红,杨欣,王蒙,等.黄芩与黄柏协同保护黄药子致肝毒性的实验研究[J].中国中药杂志,2016,41(5):898-903.
[8]曹亮亮,王文晓,张丽,等.基于“有故无殒”思想的醋甘遂毒性研究[J].中国中药杂志,2015,40(16):3249-3255.
[9]侯甲福,郑著家,刘世娟,等.锦花清热胶囊对动物急性及长期毒性研究[J].毒理学杂志,2016,30(2):172-173.
[10]赵子婧,巩政,史少泽,等.补骨脂及其与甘草配伍单次给药大鼠的毒代动力学及肝肾毒性初探[J].中国中药杂志,2015,40(11):2221-2226.
[11]程生辉,张妍妍,李会芳,等.基于黄疸模型大鼠的栀子苷急性肝肾毒性研究[J].中国实验方剂学杂志,2015,21(4):174-178.
[12]郭晓,王萌,朱彦,等.中药肾毒性机制研究现状及评价方法研究进展[J].中草药,2015,46(23):3581-3591.
[13]CHEN L,LI J S,KE X,et al.The therapeutic effects of Periploca forrestii Schltr.Stem extracts on collagen-induced arthritis by inhibiting the activation of Src/NF-κB signaling pathway in rats[J]. J Ethnopharmacology,2017,202:12-19.
[14]李维熙,李怡芳,何蓉蓉.儿茶素类化合物抗氧化评价方法及作用机制的研究现状[J].中药新药与临床药理,2016,27(2):295-303.
[15]陈凡,马善丽,许颖,等.超高压处理对豆乳总多酚、类黄酮含量及其抗氧化性的影响[J].大豆科学,2011,30(2):310-313.