梭鱼草化感物质丁二酸对微囊藻和栅藻生长及竞争的影响
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摘要
为了解梭鱼草(Pontederia cordata)根茎有机酸类化感物质对铜绿微囊藻(Micro-cystis aeruginosa,M)和斜生栅藻(Scenedesmus obliquus,S)生长和竞争关系的影响,将不同浓度丁二酸作用于两种藻的单种培养和共培养,即铜绿微囊藻与斜生栅藻初始比例为1∶0(100%M)、1∶1(50%M+50%S)、2∶3(40%M+60%S)、3∶2(60%M+40%S)、0∶1(100%S)。结果表明:丁二酸对铜绿微囊藻和斜生栅藻均有抑制作用,抑制能力与浓度呈正相关;无化感物质作用下,单种培养和共培养中铜绿微囊藻生长能力均强于斜生栅藻; 60mg·L-1丁二酸处理下,40%M+60%S、50%M+50%S培养组中β(斜生栅藻对铜绿微囊藻的抑制参数)>0,60%M+40%S培养组中β<0,表明藻类初始比例影响蓝绿藻之间竞争;丁二酸可改变蓝绿藻之间竞争关系,低浓度(20、40 mg·L-1)作用下,表现为铜绿微囊藻抑制斜生栅藻,斜生栅藻促进铜绿微囊藻,高浓度(60、80 mg·L-1)作用下,表现为两者相互抑制,表明两种藻之间竞争加强。
To understand the impacts of organic acids released by roots of Pontederia cordata on growth and competition of Microcystis aeruginosa and Scenedesmus obliquus,single culture and mix-culture of the two species were treated with succinic acid at different concentrations in a laboratory control environment. The initial ratio of M. aeruginosa to S. obliquus in BG11 medium was1 ∶ 0( 100% M),1 ∶ 1( 50% M+50% S),2 ∶ 3( 40% M+60% S),3 ∶ 2( 60% M + 40%S) and 0 ∶ 1( 100% S). Succinic acid exhibited a strong inhibitory effect on growth of M. aeruginosa and S. obliquus,and the magnitude of such effect was positively correlated with the concentration of succinic acid. In the absence of succinic acid,M. aeruginosa cultured with or without S. obliquus had a faster growth rate than S. obliquus. With the addition of 60 mg·L-1 succinic acid,β( an inhibitory parameter of S. obliquus to M. aeruginosa) was more than 0 when the ratio of M. aeruginosa to S. obliquus was 2 ∶ 3 or 1 ∶ 1 and less than 0 when the ratio of M. aeruginosa to S. obliquus was 3 ∶ 2,indicating that the inhibitory effect of one to another varied with the initial proportion of the two species. The succinic acid could change the competition between M.aeruginosa and S. obliquus. Under low concentrations( 20,40 mg·L-1),M. aeruginosa inhibited S. obliquus,but S. obliquus promoted M. aeruginosa. Under high concentrations( 60,80 mg·L-1),the performance of both species was mutually inhibited,indicating an intensified competition.
To understand the impacts of organic acids released by roots of Pontederia cordata on growth and competition of Microcystis aeruginosa and Scenedesmus obliquus,single culture and mix-culture of the two species were treated with succinic acid at different concentrations in a laboratory control environment. The initial ratio of M. aeruginosa to S. obliquus in BG11 medium was1 ∶ 0( 100% M),1 ∶ 1( 50% M+50% S),2 ∶ 3( 40% M+60% S),3 ∶ 2( 60% M + 40%S) and 0 ∶ 1( 100% S). Succinic acid exhibited a strong inhibitory effect on growth of M. aeruginosa and S. obliquus,and the magnitude of such effect was positively correlated with the concentration of succinic acid. In the absence of succinic acid,M. aeruginosa cultured with or without S. obliquus had a faster growth rate than S. obliquus. With the addition of 60 mg·L-1 succinic acid,β( an inhibitory parameter of S. obliquus to M. aeruginosa) was more than 0 when the ratio of M. aeruginosa to S. obliquus was 2 ∶ 3 or 1 ∶ 1 and less than 0 when the ratio of M. aeruginosa to S. obliquus was 3 ∶ 2,indicating that the inhibitory effect of one to another varied with the initial proportion of the two species. The succinic acid could change the competition between M.aeruginosa and S. obliquus. Under low concentrations( 20,40 mg·L-1),M. aeruginosa inhibited S. obliquus,but S. obliquus promoted M. aeruginosa. Under high concentrations( 60,80 mg·L-1),the performance of both species was mutually inhibited,indicating an intensified competition.
引文
晁建颖,颜润润,张毅敏.2011.不同温度下铜绿微囊藻和斜生栅藻的最佳生长率及竞争作用.生态与农村环境学报,27(2):53-57.
陈洁,段舜山,李爱芬,等.2003.眼点拟微绿球藻与扁藻在不同接种比例下的竞争.海洋科学,27(5):73-76.
陈德辉,刘永定,袁峻峰,等.1999.微囊藻和栅藻共培养实验及其竞争参数的计算.生态学报,19(6):908-913.
陈德辉,章宗涉,刘永定,等.2000.微囊藻栅藻资源竞争的动力学过程.Ⅰ.光能和磷营养的半饱和参数及其生长率动态.环境科学学报,20(3):349-354.
董颖娜,冯彬,王冰璇,等.2018.香蒲水浸提液对铜绿微囊藻的化感作用.生态学杂志,37(2):498-505.
董云伟,董双林,刘相义.2004.不同起始浓度对塔玛亚历山大藻和赤潮异弯藻种群竞争的影响.中国海洋大学学报:自然科学版,34(6):964-968.
方婷轩,马增岭.2018.铜绿微囊藻(Microcystis aeruginosa)次生代谢物对普通小球藻(Chlorella vulgaris)生长及有效量子产率的影响.湖泊科学,30(3):732-740.
洪喻,胡洪营.2009.水生植物化感抑藻作用研究与应用.科学通报,54(3):287-293.
孟顺龙,裘丽萍,胡庚东,等.2012.氮磷比对两种蓝藻生长及竞争的影响.农业环境科学学报,31(7):1438-1444.
倪妍,万金保,赖劲虎,等.2014.硝磺草酮对微囊藻和四尾栅藻光合作用及竞争的影响.农业环境科学学报,33(12):2436-2443.
彭桂莹,陈永玲,韩玉珍,等.2016.乳酸对铜绿微囊藻的抑藻效应及机理.中国环境科学,36(4):1167-1172.
苏文,陈洁,张胜鹏,等.2017.水稻秸秆浸泡液对蓝藻和绿藻生长选择性抑制作用.环境科学,38(7):2901-2909.
谭啸,戴凯文,段志鹏,等.2018.萘对铜绿微囊藻和聚球藻生长及叶绿素荧光影响的比较.河海大学学报:自然科学版,46(2):115-121.
唐超,喻慧,杨传俊,等.2017.电磁环境胁迫对铜绿微囊藻氧化应激和光合固碳酶的影响作用研究.环境科学学报,37(8):3194-3200.
田如男,孙欣欣,魏勇,等.2011.水生花卉对铜绿微囊藻、斜生栅藻和小球藻生长的影响.生态学杂志,30(12):2732-2738.
万蕾,朱伟,赵联芳.2007.氮磷对微囊藻和栅藻生长及竞争的影响.环境科学,28(6):1230-1235.
王珂,逄勇,高光.2006.不同扰动条件下微囊藻和栅藻竞争能力的比较.环境科技,19(2):40-42.
薛凌展,黄种持,林泽,等.2010.铜绿微囊藻和普通小球藻在不同p H下生长特性及竞争参数计算.福建农业学报,25(2):142-148.
杨璨.2014.三种环境内分泌干扰物对铜绿微囊藻生长及生理的影响(硕士学位论文).上海:上海交通大学.
杨苏文,姜霞,金相灿.2007.HCO3-对铜绿微囊藻、四尾栅藻和小环藻增长特性及竞争行为的影响.生态环境学报,16(2):347-351.
张民,孔繁翔,史小丽,等.2007.铜绿微囊藻在竞争生长条件下对氧化还原电位降低的响应.湖泊科学,19(2):118-124.
张景雯,田如男.2018.四种植物对模拟的城市景观污水的净化效果.湿地科学,16(1):85-92.
赵联芳,丁小燕,陆琳,等.2014.营养盐及Ca2+对微囊藻和栅藻生长及竞争的影响.环境科学与技术,37(1):13-17.
赵晓东,潘江,李金页,等.2011.铜绿微囊藻和斜生栅藻非稳态营养盐限制条件下的生长竞争特性.生态学报,31(13):3710-3719.
郑忠明,白培峰,陆开宏,等.2008.铜绿微囊藻和四尾栅藻在不同温度下的生长特性及竞争参数计算.水生生物学报,32(5):720-727.
Amano Y,Sakai Y,Sekiya T,et al.2010.Effect of phosphorus fluctuation caused by river water dilution in eutrophic lake on competition between blue-green alga Microcystis aeruginosa and diatom Cyclotella sp.Journal of Environmental Sciences,22:1666-1673.
Amano Y,Takahashi K,Machida M.2012.Competition between the cyanobacterium Microcystis aeruginosa,and the diatom Cyclotella sp.under nitrogen-limited condition caused by dilution in eutrophic lake.Journal of Applied Phycology,24:965-971.
Chen JQ,Guo RX.2014.Inhibition effect of green alga on cyanobacteria by the interspecies interactions.International Journal of Environmental Science&Technology,11:839-842.
Dai RH,Liu HJ,Qu JH,et al.2008.Relationship of energy charge and toxin content of Microcystis aeruginosa in nitrogen-limited or phosphorous-limited cultures.Toxicon,51:649-658.
He Y,Cheng L,Tian Y,et al.2016.Effects of submerged macrophyte Ceratophyllum demersum on ROS generation and cell damage in Microcystis aeruginosa.Allelopathy Journal,38:229-238.
Keating KI.1978.Blue-green algal inhibition of diatom growth:Transition from mesotrophic to eutrophic community structure.Science,199:971-973.
Margalef R.1975.External factors and ecosystem stability.Schweizerische Zeitschrift für Hydrologie,37:102-117.
Marinho MM,Souza MBG,Lürling M.2013.Light and phosphate competition between Cylindrospermopsis raciborskii and Microcystis aeruginosa is strain dependent.Microbial Ecology,66:479-488.
Mello MM,Soares MCS,Roland F,et al.2012.Growth inhibition and colony formation in the cyanobacterium Microcystis aeruginosa induced by the cyanobacterium Cylindrospermopsis raciborskii.Journal of Plankton Research,34:987-994.
Nakai S,Seto N,Asaoka S,et al.2013.Impact of the allelopathic effect of a submerged Macrophyte Myriophyllum spicatum on the competition between the cyanobacterium Microcystis aeruginosa and the green alga Scenedesmus obliquus.Journal of Japan Society on Water Environment,36:85-90.
Paerl HW,Moisander PH,Dyble J,et al.2001.Harmful freshwater algal blooms,with an emphasis on cyanobacteria.The Scientific World Journal,1:76.
Qiu XC,Yamasaki Y,Shimasaki Y,et al.2012.Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact.Marine Ecology Progress Series,446:107-118.
Rzymski P,Poniedialek B,Kokocinski M,et al.2014.Interspecific allelopathy in cyanobacteria:Cylindrospermopsin and Cylindrospermopsis raciborskii effect on the growth and metabolism of Microcystis aeruginosa.Harmful Algae,35:1-8.
Shao JH,Peng L,Luo S,et al.2013.First report on the allelopathic effect of Tychonema bourrellyi(Cyanobacteria)against Microcystis aeruginosa(Cyanobacteria).Journal of Applied Phycology,25:1567-1573.
Volterra V.1926.Fluctuations in the abundance of a species considered mathematically.Nature,118:558-560.
Wang J,Zhu JY,Liu SP,et al.2011.Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes.Chemosphere,85:977-982.
Yang J,Deng XR,Xian QM,et al.2014.Allelopathic effect of Microcystis aeruginosa on Microcystis wesenbergii:Microcystin-LR as a potential allelochemical.Hydrobiologia,727:65-73.
Zhu JY,Liu BY,Wang J,et al.2010.Study on the mechanism of allelopathic influence on cyanobacteria and chlorophytes by submerged macrophyte(Myriophyllum spicatum)and its secretion.Aquatic Toxicology,98:196-203.
陈洁,段舜山,李爱芬,等.2003.眼点拟微绿球藻与扁藻在不同接种比例下的竞争.海洋科学,27(5):73-76.
陈德辉,刘永定,袁峻峰,等.1999.微囊藻和栅藻共培养实验及其竞争参数的计算.生态学报,19(6):908-913.
陈德辉,章宗涉,刘永定,等.2000.微囊藻栅藻资源竞争的动力学过程.Ⅰ.光能和磷营养的半饱和参数及其生长率动态.环境科学学报,20(3):349-354.
董颖娜,冯彬,王冰璇,等.2018.香蒲水浸提液对铜绿微囊藻的化感作用.生态学杂志,37(2):498-505.
董云伟,董双林,刘相义.2004.不同起始浓度对塔玛亚历山大藻和赤潮异弯藻种群竞争的影响.中国海洋大学学报:自然科学版,34(6):964-968.
方婷轩,马增岭.2018.铜绿微囊藻(Microcystis aeruginosa)次生代谢物对普通小球藻(Chlorella vulgaris)生长及有效量子产率的影响.湖泊科学,30(3):732-740.
洪喻,胡洪营.2009.水生植物化感抑藻作用研究与应用.科学通报,54(3):287-293.
孟顺龙,裘丽萍,胡庚东,等.2012.氮磷比对两种蓝藻生长及竞争的影响.农业环境科学学报,31(7):1438-1444.
倪妍,万金保,赖劲虎,等.2014.硝磺草酮对微囊藻和四尾栅藻光合作用及竞争的影响.农业环境科学学报,33(12):2436-2443.
彭桂莹,陈永玲,韩玉珍,等.2016.乳酸对铜绿微囊藻的抑藻效应及机理.中国环境科学,36(4):1167-1172.
苏文,陈洁,张胜鹏,等.2017.水稻秸秆浸泡液对蓝藻和绿藻生长选择性抑制作用.环境科学,38(7):2901-2909.
谭啸,戴凯文,段志鹏,等.2018.萘对铜绿微囊藻和聚球藻生长及叶绿素荧光影响的比较.河海大学学报:自然科学版,46(2):115-121.
唐超,喻慧,杨传俊,等.2017.电磁环境胁迫对铜绿微囊藻氧化应激和光合固碳酶的影响作用研究.环境科学学报,37(8):3194-3200.
田如男,孙欣欣,魏勇,等.2011.水生花卉对铜绿微囊藻、斜生栅藻和小球藻生长的影响.生态学杂志,30(12):2732-2738.
万蕾,朱伟,赵联芳.2007.氮磷对微囊藻和栅藻生长及竞争的影响.环境科学,28(6):1230-1235.
王珂,逄勇,高光.2006.不同扰动条件下微囊藻和栅藻竞争能力的比较.环境科技,19(2):40-42.
薛凌展,黄种持,林泽,等.2010.铜绿微囊藻和普通小球藻在不同p H下生长特性及竞争参数计算.福建农业学报,25(2):142-148.
杨璨.2014.三种环境内分泌干扰物对铜绿微囊藻生长及生理的影响(硕士学位论文).上海:上海交通大学.
杨苏文,姜霞,金相灿.2007.HCO3-对铜绿微囊藻、四尾栅藻和小环藻增长特性及竞争行为的影响.生态环境学报,16(2):347-351.
张民,孔繁翔,史小丽,等.2007.铜绿微囊藻在竞争生长条件下对氧化还原电位降低的响应.湖泊科学,19(2):118-124.
张景雯,田如男.2018.四种植物对模拟的城市景观污水的净化效果.湿地科学,16(1):85-92.
赵联芳,丁小燕,陆琳,等.2014.营养盐及Ca2+对微囊藻和栅藻生长及竞争的影响.环境科学与技术,37(1):13-17.
赵晓东,潘江,李金页,等.2011.铜绿微囊藻和斜生栅藻非稳态营养盐限制条件下的生长竞争特性.生态学报,31(13):3710-3719.
郑忠明,白培峰,陆开宏,等.2008.铜绿微囊藻和四尾栅藻在不同温度下的生长特性及竞争参数计算.水生生物学报,32(5):720-727.
Amano Y,Sakai Y,Sekiya T,et al.2010.Effect of phosphorus fluctuation caused by river water dilution in eutrophic lake on competition between blue-green alga Microcystis aeruginosa and diatom Cyclotella sp.Journal of Environmental Sciences,22:1666-1673.
Amano Y,Takahashi K,Machida M.2012.Competition between the cyanobacterium Microcystis aeruginosa,and the diatom Cyclotella sp.under nitrogen-limited condition caused by dilution in eutrophic lake.Journal of Applied Phycology,24:965-971.
Chen JQ,Guo RX.2014.Inhibition effect of green alga on cyanobacteria by the interspecies interactions.International Journal of Environmental Science&Technology,11:839-842.
Dai RH,Liu HJ,Qu JH,et al.2008.Relationship of energy charge and toxin content of Microcystis aeruginosa in nitrogen-limited or phosphorous-limited cultures.Toxicon,51:649-658.
He Y,Cheng L,Tian Y,et al.2016.Effects of submerged macrophyte Ceratophyllum demersum on ROS generation and cell damage in Microcystis aeruginosa.Allelopathy Journal,38:229-238.
Keating KI.1978.Blue-green algal inhibition of diatom growth:Transition from mesotrophic to eutrophic community structure.Science,199:971-973.
Margalef R.1975.External factors and ecosystem stability.Schweizerische Zeitschrift für Hydrologie,37:102-117.
Marinho MM,Souza MBG,Lürling M.2013.Light and phosphate competition between Cylindrospermopsis raciborskii and Microcystis aeruginosa is strain dependent.Microbial Ecology,66:479-488.
Mello MM,Soares MCS,Roland F,et al.2012.Growth inhibition and colony formation in the cyanobacterium Microcystis aeruginosa induced by the cyanobacterium Cylindrospermopsis raciborskii.Journal of Plankton Research,34:987-994.
Nakai S,Seto N,Asaoka S,et al.2013.Impact of the allelopathic effect of a submerged Macrophyte Myriophyllum spicatum on the competition between the cyanobacterium Microcystis aeruginosa and the green alga Scenedesmus obliquus.Journal of Japan Society on Water Environment,36:85-90.
Paerl HW,Moisander PH,Dyble J,et al.2001.Harmful freshwater algal blooms,with an emphasis on cyanobacteria.The Scientific World Journal,1:76.
Qiu XC,Yamasaki Y,Shimasaki Y,et al.2012.Allelopathy of the raphidophyte Heterosigma akashiwo against the dinoflagellate Akashiwo sanguinea is mediated via allelochemicals and cell contact.Marine Ecology Progress Series,446:107-118.
Rzymski P,Poniedialek B,Kokocinski M,et al.2014.Interspecific allelopathy in cyanobacteria:Cylindrospermopsin and Cylindrospermopsis raciborskii effect on the growth and metabolism of Microcystis aeruginosa.Harmful Algae,35:1-8.
Shao JH,Peng L,Luo S,et al.2013.First report on the allelopathic effect of Tychonema bourrellyi(Cyanobacteria)against Microcystis aeruginosa(Cyanobacteria).Journal of Applied Phycology,25:1567-1573.
Volterra V.1926.Fluctuations in the abundance of a species considered mathematically.Nature,118:558-560.
Wang J,Zhu JY,Liu SP,et al.2011.Generation of reactive oxygen species in cyanobacteria and green algae induced by allelochemicals of submerged macrophytes.Chemosphere,85:977-982.
Yang J,Deng XR,Xian QM,et al.2014.Allelopathic effect of Microcystis aeruginosa on Microcystis wesenbergii:Microcystin-LR as a potential allelochemical.Hydrobiologia,727:65-73.
Zhu JY,Liu BY,Wang J,et al.2010.Study on the mechanism of allelopathic influence on cyanobacteria and chlorophytes by submerged macrophyte(Myriophyllum spicatum)and its secretion.Aquatic Toxicology,98:196-203.