“龙榛1号”杂交榛根蘖育苗生根过程中营养物质的变化
|
摘要
以"龙榛1号"杂交榛根蘖为试材,利用(500、750、1 000、1 250mg·L-1)IBA、ABT和GGR处理根蘖基部,研究了激素及浓度处理对根蘖育苗生根的影响,以期探讨生根过程中营养物质含量的动态变化。结果表明:不同激素处理中以1 000mg·L-1 IBA处理的生根效果最好,生根率平均87.92%,一级侧根数量、长度和粗度显著地高于其它处理和对照。在生根过程中,IBA处理的根蘖基部可溶性糖和可溶性蛋白质含量呈"下降-上升-下降"的趋势,在不定根形成期达到峰值;而全氮含量变化为先上升后下降,含量整体低于对照;C/N比值的变化呈先下降后上升趋势。IBA处理枝条可促进可溶性糖的积累,降低全氮含量,提高C/N比值,有利于诱导根原基和不定根的形成。
The basal parts of the germinating strips of C.heterophylla×C.avellana were treated with IBA,ABT and GGR concentrations(500,750,1 000,1 250 mg·L~(-1)).The effects of hormone and concentration on rooting of root-tiller seedlings were studied,and the dynamic changes of nutrient contents during rooting were discussed.The results showed that 1 000mg·L~(-1 )IBA treatment had the best rooting effect,with an average rooting rate of 87.92%.The number,length and thickness of primary lateral roots were significantly higher than those of other treatments and controls.During the rooting process,the soluble sugar and soluble protein content in the basal part of the sprouting strip treated with IBA showed a trend of‘descending-ascending-descending’and reached a peak at the adventitious root formation stage,while the total nitrogen content increased first and then decreased,and the content was lower than the control as a whole;the change of C/N ratio showed a trend of descending first and then rising.IBA treatment could promote the accumulation of soluble sugar,reduce the total nitrogen content,and increase the C/N ratio,which was conducive to the formation of root primordia and adventitious roots.
The basal parts of the germinating strips of C.heterophylla×C.avellana were treated with IBA,ABT and GGR concentrations(500,750,1 000,1 250 mg·L~(-1)).The effects of hormone and concentration on rooting of root-tiller seedlings were studied,and the dynamic changes of nutrient contents during rooting were discussed.The results showed that 1 000mg·L~(-1 )IBA treatment had the best rooting effect,with an average rooting rate of 87.92%.The number,length and thickness of primary lateral roots were significantly higher than those of other treatments and controls.During the rooting process,the soluble sugar and soluble protein content in the basal part of the sprouting strip treated with IBA showed a trend of‘descending-ascending-descending’and reached a peak at the adventitious root formation stage,while the total nitrogen content increased first and then decreased,and the content was lower than the control as a whole;the change of C/N ratio showed a trend of descending first and then rising.IBA treatment could promote the accumulation of soluble sugar,reduce the total nitrogen content,and increase the C/N ratio,which was conducive to the formation of root primordia and adventitious roots.
引文
[1]宋锋惠,史彦江,卡得尔.大果杂交榛子引种及优良品种的选育[J].东北林业大学学报,2007,35(5):87-89.
[2]马庆华,王贵禧,梁维坚,等.我国榛属植物种质资源的研究、利用与创新[J].果树学报,2013,30(1):159-164.
[3]车树理,刘玲玲,卢瑞林.榛子在甘肃定西的引种表现及栽培技术要点[J].山西果树,2009(5):20-21.
[4]万建华,陈炜青,姜成英,等.杂交榛子在甘肃的引种表现[J].甘肃林业科技,2010,35(1):74-76.
[5]张洪.平欧杂种榛在北京地区的引种表现[J].中国果树,2010(6):40-43.
[6]宋锋惠,史彦江,卡得尔.大果杂交榛子引种及优良品种的选育[J].东北林业大学学报,2007,35(5):87-89.
[7]宋锋惠,罗青红,史彦江,等.平欧杂交榛4个品种光合特性的比较研究[J].新疆农业科学,2010,47(5):876-881.
[8]陈凤,苏淑钗,白倩.平欧杂交榛枝条主要性状因子间的相关分析[J].经济林研究,2013,31(2):20-25.
[9]罗青红,史彦江,宋锋惠,等.不同产地杂交榛果实品质比较分析[J].食品科学,2013,34(3):50-54.
[10]龙作义,逄宏扬,李红莉,等.平榛与龙榛的远缘杂交F1代田间评比试验初报[J].经济林研究,2014,32(2):139-143.
[11]李春牛,董凤祥,王贵禧,等.平欧杂交榛抗抽条能力及抽条临界含水量研究[J].林业科学研究,2010,23(3):330-335.
[12]寇云玲,罗青红,宋锋惠,等.新疆杂交榛叶片解剖结构研究[J].果树学报,2012,29(6):1063-1068.
[13]吕跃东,董凤祥,王贵禧,等.平欧杂交榛抗寒性综合评价体系的建立与应用[J].林业科学,2008,44(9):31-35.
[14]宋锋惠,罗达,史彦江,等.不同修枝强度对平欧杂种榛生长与结实特性的影响[J].东北林业大学学报,2017,45(11):17-20.
[15]艾婷婷.不同缢根材料对平欧杂种榛苗木繁育的影响[J].防护林科技,2017(5):18-20.
[16]胡俊达.平欧杂交榛优质丰产栽培技术[J].河北果树,2017(3):43-44.
[17]潘越,史彦江,陈淑英,等.喷施叶面肥对平欧杂种榛“新榛1号”嫩枝扦插的影响[J].江西农业大学学报,2016,38(5):920-926.
[18]艾婷婷.不同繁苗时间对沙地平欧杂种榛压条苗生根的影响[J].防护林科技,2017(7):21-22.
[19]梁春莉,于立杰.平欧杂交榛子压条影响因素研究[J].北方园艺,2012(3):19-21.
[20]寇云玲,史彦江,宋锋惠.生长素处理对新疆榛压条繁殖效果的研究[J].新疆农业大学学报,2011,34(6):486-489.
[21]张思敏,宋敏丽,曹艳花,等.激素对平欧杂种榛硬枝容器扦插生根的影响[J].北方园艺,2017(11):71-75.
[22]田雪邻,史彦江,宋锋惠,等.不同处理对平欧杂种榛黄化萌条扦插生根的影响[J].西北林学院学报,2018,33(1):126-132.
[23]赵蕾,陶秀冬,宋锋惠,等.不同处理对平欧杂种榛嫩枝扦插生根效果的影响[J].东北林业大学学报,2014,42(12):22-25.
[24]赵蕾,宋锋惠,史彦江,等.吲哚丁酸对平欧杂种榛嫩枝扦插生根效果的影响[J].北方园艺,2015(19):22-26.
[25]李大威.榛子扦插繁殖技术及不定根发生机理研究[D].北京:北京林业大学,2008.
[26]扈红军,曹帮华,尹伟伦,等.榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术[J].林业科学,2008,44(6):60-65.
[27]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[28]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2003,129-130.
[29]单守田,刘明国,王玲,等.北美香柏插穗生根过程中营养物质的变化[J].辽宁林业科技,2012(1):1-3.
[30]李千惠,范俊俊,赵明明,等.外源激素在金雀花扦插生根进程中的调节机制[J].中南林业科技大学学报,2017,37(10):54-60.
[31]刘玉艳,于凤鸣,于娟.IBA对含笑扦插生根影响初探[J].河北农业大学学报,2003,26(2):25-29.
[32]敖红,王昆,陈一凌,等.长白落叶松插穗内的营养物质及其对扦插生根的影响[J].植物研究,2002,22(3):301-304.
[33]赵云龙,陈训,李朝婵.糙叶杜鹃扦插生根过程中生理生化分析[J].林业科学,2013,49(6):45-49.
[34]宋丽红.光叶褚微体快繁技术与扦插生根机理研究[D].泰安:山东农业大学,2005.
[2]马庆华,王贵禧,梁维坚,等.我国榛属植物种质资源的研究、利用与创新[J].果树学报,2013,30(1):159-164.
[3]车树理,刘玲玲,卢瑞林.榛子在甘肃定西的引种表现及栽培技术要点[J].山西果树,2009(5):20-21.
[4]万建华,陈炜青,姜成英,等.杂交榛子在甘肃的引种表现[J].甘肃林业科技,2010,35(1):74-76.
[5]张洪.平欧杂种榛在北京地区的引种表现[J].中国果树,2010(6):40-43.
[6]宋锋惠,史彦江,卡得尔.大果杂交榛子引种及优良品种的选育[J].东北林业大学学报,2007,35(5):87-89.
[7]宋锋惠,罗青红,史彦江,等.平欧杂交榛4个品种光合特性的比较研究[J].新疆农业科学,2010,47(5):876-881.
[8]陈凤,苏淑钗,白倩.平欧杂交榛枝条主要性状因子间的相关分析[J].经济林研究,2013,31(2):20-25.
[9]罗青红,史彦江,宋锋惠,等.不同产地杂交榛果实品质比较分析[J].食品科学,2013,34(3):50-54.
[10]龙作义,逄宏扬,李红莉,等.平榛与龙榛的远缘杂交F1代田间评比试验初报[J].经济林研究,2014,32(2):139-143.
[11]李春牛,董凤祥,王贵禧,等.平欧杂交榛抗抽条能力及抽条临界含水量研究[J].林业科学研究,2010,23(3):330-335.
[12]寇云玲,罗青红,宋锋惠,等.新疆杂交榛叶片解剖结构研究[J].果树学报,2012,29(6):1063-1068.
[13]吕跃东,董凤祥,王贵禧,等.平欧杂交榛抗寒性综合评价体系的建立与应用[J].林业科学,2008,44(9):31-35.
[14]宋锋惠,罗达,史彦江,等.不同修枝强度对平欧杂种榛生长与结实特性的影响[J].东北林业大学学报,2017,45(11):17-20.
[15]艾婷婷.不同缢根材料对平欧杂种榛苗木繁育的影响[J].防护林科技,2017(5):18-20.
[16]胡俊达.平欧杂交榛优质丰产栽培技术[J].河北果树,2017(3):43-44.
[17]潘越,史彦江,陈淑英,等.喷施叶面肥对平欧杂种榛“新榛1号”嫩枝扦插的影响[J].江西农业大学学报,2016,38(5):920-926.
[18]艾婷婷.不同繁苗时间对沙地平欧杂种榛压条苗生根的影响[J].防护林科技,2017(7):21-22.
[19]梁春莉,于立杰.平欧杂交榛子压条影响因素研究[J].北方园艺,2012(3):19-21.
[20]寇云玲,史彦江,宋锋惠.生长素处理对新疆榛压条繁殖效果的研究[J].新疆农业大学学报,2011,34(6):486-489.
[21]张思敏,宋敏丽,曹艳花,等.激素对平欧杂种榛硬枝容器扦插生根的影响[J].北方园艺,2017(11):71-75.
[22]田雪邻,史彦江,宋锋惠,等.不同处理对平欧杂种榛黄化萌条扦插生根的影响[J].西北林学院学报,2018,33(1):126-132.
[23]赵蕾,陶秀冬,宋锋惠,等.不同处理对平欧杂种榛嫩枝扦插生根效果的影响[J].东北林业大学学报,2014,42(12):22-25.
[24]赵蕾,宋锋惠,史彦江,等.吲哚丁酸对平欧杂种榛嫩枝扦插生根效果的影响[J].北方园艺,2015(19):22-26.
[25]李大威.榛子扦插繁殖技术及不定根发生机理研究[D].北京:北京林业大学,2008.
[26]扈红军,曹帮华,尹伟伦,等.榛子嫩枝扦插生根相关氧化酶活性变化及繁殖技术[J].林业科学,2008,44(6):60-65.
[27]李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2000.
[28]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2003,129-130.
[29]单守田,刘明国,王玲,等.北美香柏插穗生根过程中营养物质的变化[J].辽宁林业科技,2012(1):1-3.
[30]李千惠,范俊俊,赵明明,等.外源激素在金雀花扦插生根进程中的调节机制[J].中南林业科技大学学报,2017,37(10):54-60.
[31]刘玉艳,于凤鸣,于娟.IBA对含笑扦插生根影响初探[J].河北农业大学学报,2003,26(2):25-29.
[32]敖红,王昆,陈一凌,等.长白落叶松插穗内的营养物质及其对扦插生根的影响[J].植物研究,2002,22(3):301-304.
[33]赵云龙,陈训,李朝婵.糙叶杜鹃扦插生根过程中生理生化分析[J].林业科学,2013,49(6):45-49.
[34]宋丽红.光叶褚微体快繁技术与扦插生根机理研究[D].泰安:山东农业大学,2005.