摘要
目的:研究痧脊经筋疗法对兔膝骨性关节炎软骨的影响,探讨非药物治疗膝骨性关节炎的作用机制。方法:采用管型石膏过伸位制动兔左后膝骨关节,并按照随机数字化法分组,造模2个月后开始痧脊经筋治疗,2个月后将兔处死,取材进行大体观察、Mankin评分,实时荧光定量PCR检测软骨TGF-β1、Smad3 mRNA,ELISA法检测关节液白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)。结果:痧脊经筋组和氨基葡萄糖组软骨TGF-β1、Smad3 mRNA高于模型对照组(P<0.05),关节液中IL-1β、IL-6、TNF-α的水平低于模型对照组(P<0.05)。与氨基葡萄糖组比较,痧脊经筋组TGF-β1、Smad3 mRNA较高(P<0.05)。结论:痧脊经筋疗法可使软骨退变的部分因素得到控制,是值得推广的新型非药物治疗方法。
Objective: To study 'scraping spinal-acupoints and meridian-muscle' therapy on knee osteoarthritis in rabbits,and explore the mechanism of non-drug treatment of knee osteoarthritis. Methods: The rabbit's left posterior knee joint was immobilized by hyperextension of tubular plaster and was grouped according to random digitization. The rabbits were sacrificed after 2 months. The rabbits were observed and scored by Mankin. The mRNA of TGF-β1 and Smad3 in cartilage and the levels of IL-1β, IL-6 and TNF-α in joint fluid were detected. Results: TGF-β1 and Smad3 in scraping spinal-acupoints and glucosamine group cartilage were higher than model control group(P<0.05). The levels of IL-1β, IL-6 and TNF-α in synovial fluid were lower than model control group(P<0.05). The expressiones of TGF-β1 and Smad3 mRNA in glucosamine group were lower than scraping spinal-acupoints and meridian-muscle group(P<0.05). Conclusion: Scraping spinal-acupoints and meridian-muscle therapy can control some factors of cartilage degeneration, it is a new non-drug treatment method which is worth popularizing.
引文
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