黄瓜发芽期耐热性评价及全基因组关联分析
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摘要
高温会降低作物种子活力,减慢或完全抑制发芽,是制约黄瓜生产的重要非生物胁迫之一。本研究以96份黄瓜核心种质为试材,在42℃下进行发芽试验,利用相对发芽率、相对发芽势、相对发芽指数和相对胚根长度计算出的综合评价值,对核心种质发芽期耐热性进行鉴定评价;并利用重测序数据,对核心种质发芽期的耐热性进行全基因组关联分析。通过鉴定,筛选出了2份耐热性极强的材料CG109和CG110,综合评价值均大于0.9。通过全基因组关联分析,检测到5个与相对发芽率关联的位点,分别是GGP4.1、GGP4.2、GGP5.1、GGP5.2和GGR6.1;2个与相对发芽势关联的位点,分别是GGV4.1和GGV5.1;2个与相对发芽指数关联的位点,分别是GGI4.1和GGI5.1;2个与相对胚根长度关联的位点,分别是GRL6.1和GRL7.1。在以上位点中,GGI4.1、GGV4.1和GGP4.1均位于Chr.4的同一区间;GGI5.1、GGV5.1和GGP5.2均位于Chr.5的同一区间。这2个区段的关联位点利用相对发芽率、相对发芽指数和相对发芽势3个指标均可检测到,由此推测该区域存在与发芽期耐热性相关的基因。
High temperature,which can reduce crop seed vigor,slow down or completely inhibit germination,is one of the important abiotic stresses that restrict cucumber production.A set of core germplasm including96 cucumber accessions were used to identify thermotolerance at high temperature 42 ℃ during the seed germination stage.The thermotolerance was evaluated using a comprehensive index,which include the relative germination percentage,relative germination vigor,relative germination index and relative radicle length.The re-sequencing data of the core germplasm were used to perform the Genome-wide association study(GWAS).We identified two accessions,CG109 and CG110,which have a pretty strong thermotolerance,with the comprehensive index of over 0.9.We detected 5 loci for relative germination percentage:GGP4.1,GGP4.2,GGP5.1,GGP5.2 and GGP6.1;2 for relative germination vigor:GGV4.1 and GGV5.1;2 for relative germination index:GGI4.1 and GGI5.1;2 for relative radicle length:GRL6.1 and GRL7.1.Among them,GGI4.1,GGV4.1 and GGP4.1 are located to the same physical region of Chr.4,and GGI5.1,GGV5.1 and GGP5.2 are located to the same region of Chr.5.The loci located in these two regions can be detected simultaneously by relative germination percentage,relative germination vigor and relative germination index three different indicators,which suggested that the two regions could include genes related to thermotolerance.
High temperature,which can reduce crop seed vigor,slow down or completely inhibit germination,is one of the important abiotic stresses that restrict cucumber production.A set of core germplasm including96 cucumber accessions were used to identify thermotolerance at high temperature 42 ℃ during the seed germination stage.The thermotolerance was evaluated using a comprehensive index,which include the relative germination percentage,relative germination vigor,relative germination index and relative radicle length.The re-sequencing data of the core germplasm were used to perform the Genome-wide association study(GWAS).We identified two accessions,CG109 and CG110,which have a pretty strong thermotolerance,with the comprehensive index of over 0.9.We detected 5 loci for relative germination percentage:GGP4.1,GGP4.2,GGP5.1,GGP5.2 and GGP6.1;2 for relative germination vigor:GGV4.1 and GGV5.1;2 for relative germination index:GGI4.1 and GGI5.1;2 for relative radicle length:GRL6.1 and GRL7.1.Among them,GGI4.1,GGV4.1 and GGP4.1 are located to the same physical region of Chr.4,and GGI5.1,GGV5.1 and GGP5.2 are located to the same region of Chr.5.The loci located in these two regions can be detected simultaneously by relative germination percentage,relative germination vigor and relative germination index three different indicators,which suggested that the two regions could include genes related to thermotolerance.
引文
[1]蒋先明.蔬菜栽培学各论(北方本).北京:中国农业出版社,1990:185-209Jiang X M.Vegetable Cultural(Northern version).Beijing:China Agricultural Press,1990:185-209
[2]孟令波.黄瓜(Cucumis sativus L.)耐高温种质资源筛选及耐高温特性的研究.哈尔滨:东北农业大学,2002Meng L B.Studies on high temperature tolerance character and selection of high temperature tolerance germplasm in cucumber(Cucumis sativus L.).Harbin:Northeast Agricultural University,2002
[3]Leprince O,Pellizzaro A,Berriri S,Buitink J.Late seed maturation:drying without dying.Journal of Experimental Botany,2017,68(4):827-841
[4]尚庆茂,王光耀.蔬菜抗热性鉴定方法.中国蔬菜,1996(5):49-51Shang Q M,Wang G Y.Method for identifying heat resistance of vegetables.China Vegetables,1996(5):49-51
[5]孙光玲,王海军,李海峰,蒋文修,李刚.烤烟种子活力测定方法的相关分析.烟草科技,2004(1):10Sun G L,Wang H J,Li H F,Jiang W X,Li G.Correlation analysis in seed vigour-testing methods of flue-cured tobacco.Tobacco Science&Technology,2004(1):10
[6]王红飞.黄瓜种质资源低温耐受性的鉴定评价及QTL初步定位研究.北京:中国农业科学院,2014Wang H F.Germplasm identification of tolerance to low temperature and QTL mapping for low temperature adapbility in cucumber.Beijing:Chinese Academy of Agricultural Sciences,2014
[7]聂文娟.黄瓜耐热性形态学鉴定技术研究.杨凌:西北农林科技大学,2010Nie W J.Research on technique of hot tolerance identification by morphology in cucumber.Yangling:Northwest A&F University,2010
[8]孟焕文,张彦峰,程智慧,苏菁,崔鸿文.黄瓜幼苗对热胁迫的生理反应及耐热鉴定指标筛选.西北农业学报,2000,9(1):96-99Meng H W,Zhang Y F,Cheng Z H,Su J,Cui H W.The physiological reaction to hot stress and screening of hot tolerance index in cucumber.Acta Agriculturae Borealioccidentalis Sinica,2000,9(1):96-99
[9]杨寅桂.黄瓜耐热性及热胁迫响应基因研究.南京:南京农业大学,2007Yang Y G.Study on the heat tolerance and gene of heat stress response related in cucumber.Nanjing:Nanjing Agricultural University,2007
[10]Qi J J,Liu X,Shen D,Miao H,Xie B Y,Li X X,Zeng P,Wang S H,Shang Y,Gu X F,Du Y C,Li Y,Lin T,Yuan J H,Yang X Y,Chen J F,Chen H M,Xiong X Y,Huang K,Fei ZJ,Mao L Y,Tian L,St?dler T,Renner S S,Kamoun S,Lucas W J,Zhang Z H,Huang S W.A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity.Nature Genetics,2013,45(12):1510-1515
[11]苏世闻.黄瓜种子萌发和幼苗生长的耐热性差异及其产量和生理特性.南京:南京农业大学,2014Su S W.Yield and physiological characters of cucumber varieties and heat resistant difference on its seed germination and seedling growth.Nanjing:Nanjing Agricultural University,2014
[12]Ruan S,Xue Q,Thlkowska K.Effect of priming on germination and health of rice(Oryza sativa L.)seeds.Seed Science and Technology,2002,30(2):451-458
[13]Ruan S,Xue Q,Thlkowska K.The influence of priming on germination of rice(Oryza sativa L.)seeds and seedling emergence and performance in flooded soil.Seed Science and Technology,2002,30(1):61-67
[14]Esechie H.Interaction of salinity and temperature on the germination of sorghum.Journal of Agronomy and Crop Science,1994,172:194-199
[15]何延治.农业研究中常用的几种模糊数学方法.长春:吉林大学,2006He Y Z.Some usual kinds of methods of fuzzy mathematics in agriculture research.Changchun:Jilin University,2006
[16]谢志坚.农业科学中的模糊数学方法.武汉:华中理工大学出版社,1983:99-193Xie Z J.Fuzzy mathematics method in agricultural science.Wuhan:Huazhong University of Science and Technology Press,1983:99-193
[17]李青,秦玉芝,胡新喜,丁红映,熊兴耀,王万兴.马铃薯耐盐性离体鉴定方法的建立及52份种质资源耐盐性评价.植物遗传资源学报,2018,19(4):587-597Li Q,Qin Y Z,Hu X X,Ding H Y,Xiong X Y,Wang WX.Establishment of an optimized bioassay being valuable for determining salt tolerance in 152 potato germplasm accessions.Journal of Plant Genetic Resources,2018,19(4):587-597
[18]鲁宏伟.黄瓜白粉病抗性基因挖掘.北京:中国农业科学院,2015Lu H W.Mining of the resistance gene for powdery mildew in cucumber.Beijing:Chinese Academy of Agricultural Sciences,2015
[19]张朝阳,许桂芳.利用隶属函数法对4种地被植物的耐热性综合评价.草业科学,2009,26(2):57-60Zhang C Y,Xu G F.Comprehensive evaluation of heat tolerance of four ground cover plants by membership function method.Pratacultural Science,2009,26(2):57-60
[20]Romesburg H C.Cluster analysis for researchers.NC Raleigh.USA:Lulu.com,2004
[21]许端祥,高山,林碧英,傅睿清.高温胁迫下11份瓠瓜材料的耐热性分析.福建农业学报,2011,26(4):587-590Xu D X,Gao S,Lin B Y,Fu R Q.Heat resistance of 11Lagenaria siceraria var.hispida stand germplasms.Fujian Journal of Agricultural Sciences,2011,26(4):587-590
[22]吴欣明,郭璞,池惠武,方志红,石永红,王运琦,刘建宁,王赞,王学敏.国外紫花苜蓿种质资源表型性状与品质多样性分析.植物遗传资源学报,2018(1):103-111Wu X M,Guo P,Chi H W,Fang Z H,Shi Y H,Wang Y Q,Liu J N,Wang Z,Wang X M.Diversity analysis of phenotypic traits and quality characteristics of alfalfa(Medicago sativa)introducted from abroad Germplasm resources.Journal of Plant Genetic Resources,2018(1):103-111
[23]冯晶晶,刘磊,郑峥,邓学斌,刘希艳,白金瑞,舒金帅,宋燕,李君明.醋栗番茄Solanum pimpinellifolium遗传多样性分析.植物遗传资源学报,2017,18(4):611-619Feng J J,Liu L,Zheng Z,Deng X B,Liu X Y,Bai J R,Shu J S,Song Y,Li J M.Genetic diversity of wild tomato species Solanum pimpinellifolium.Journal of Plant Genetic Resources,2017,18(4):611-619
[24]吕婧.黄瓜种质资源群体结构分析与核心种质集筛选.北京:中国农业科学院,2011Lv J.Germplasm population structure analysis and core collection of cucumber(Cucumis sativus L.).Beijing:Chinese Academy of Agricultural Sciences,2011
[25]侯锋.黄瓜.天津:天津科学技术出版社,1999:90-91Hou F.Cucumber.Tianjin:Tianjin Science and Technology Press,1999:90-91
[26]张焕欣,翁建峰,张晓聪,刘昌林,雍洪军,郝转芳,李新海.玉米穗行数全基因组关联分析.作物学报,2014,40(1):1-6Zhang H X,Weng J F,Zhang X C,Liu C L,Yong H J,Hao Z F,Li X H.Genome-wide association analysis of kernel row number in maize.Acta Agronomica Sinica,2014,40(1):1-6
[27]Yu J M,Buckler E S.Genetic association mapping and genome organization of maize.Current Opinion in Biotechnology,2006,17(2):155-160
[28]Zhu C S,Gore,M,Buckler,E S,Yu J M.Status and prospects of association mapping in plants.Plant Genome,2008,1(1):5-20
[29]Huang X H,Zhao Y,Wei X H,Li C Y,Wang A H,Zhao Q,Li W J,Guo Y L,Deng L W,Zhu C R,Fan D L,Lu Y Q,Weng Q J,Liu K Y,Zhou T Y,Jing Y F,Si L Z,Dong G J,Huang T,Lu T T,Feng Q,Qian Q,Li J Y,Han B.Genome-wide association study of flowering time and grain yield traits in a worldwide collection of rice germplasm.Nature Genetics,2011,44(1):32
[30]Buckler E S,Holland J B,Bradbury P J,Acharya C B,Brown P J,Browne C,Ersoz E,Flint-Garcia S,Garcia A,Glaubitz J C,Goodman M M,Harjes C,Guill K,Kroon D E,Larsson S,Lepak N K,Li H,Mitchell S E,Pressoir G,Peiffer J A,Rosas M O,Rocheford T R,Romay M C,Romero S,Salvo S,Sanchez V H,da Silva H S,Sun Q,Tian F,Upadyayula N,Ware D,Yates H,Yu J,Zhang Z,Kresovich S,McMullen MD.The genetic architecture of maize flowering time.Science,2009,325(5941):714
[31]Kump K L,Bradbury P J,Wisser R J,Buckler E S,Belcher A R,Oropeza-Rosas M A,Zwonitzer J C,Kresovich S,McMullen M D,Ware D,Balint-Kurti P J,Holland J B.Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population.Nature Genetics,2011,43(2):163-168
[32]杨迪菲.黄瓜耐热性QTL定位的研究.哈尔滨:东北农业大学,2006Yang D F.Mapping quantitative traits loci for the heat tolerance in cucumber.Harbin:Northeast Agricultural University,2006
[33]庄影.黄瓜耐热性遗传及QTL初步定位.扬州:扬州大学,2014Zhuang Y.Genetic analysis and preliminary QTL mapping of heat tolerance in cucumber(Cucumis sativus L.).Yangzhou:Yangzhou University,2014
[2]孟令波.黄瓜(Cucumis sativus L.)耐高温种质资源筛选及耐高温特性的研究.哈尔滨:东北农业大学,2002Meng L B.Studies on high temperature tolerance character and selection of high temperature tolerance germplasm in cucumber(Cucumis sativus L.).Harbin:Northeast Agricultural University,2002
[3]Leprince O,Pellizzaro A,Berriri S,Buitink J.Late seed maturation:drying without dying.Journal of Experimental Botany,2017,68(4):827-841
[4]尚庆茂,王光耀.蔬菜抗热性鉴定方法.中国蔬菜,1996(5):49-51Shang Q M,Wang G Y.Method for identifying heat resistance of vegetables.China Vegetables,1996(5):49-51
[5]孙光玲,王海军,李海峰,蒋文修,李刚.烤烟种子活力测定方法的相关分析.烟草科技,2004(1):10Sun G L,Wang H J,Li H F,Jiang W X,Li G.Correlation analysis in seed vigour-testing methods of flue-cured tobacco.Tobacco Science&Technology,2004(1):10
[6]王红飞.黄瓜种质资源低温耐受性的鉴定评价及QTL初步定位研究.北京:中国农业科学院,2014Wang H F.Germplasm identification of tolerance to low temperature and QTL mapping for low temperature adapbility in cucumber.Beijing:Chinese Academy of Agricultural Sciences,2014
[7]聂文娟.黄瓜耐热性形态学鉴定技术研究.杨凌:西北农林科技大学,2010Nie W J.Research on technique of hot tolerance identification by morphology in cucumber.Yangling:Northwest A&F University,2010
[8]孟焕文,张彦峰,程智慧,苏菁,崔鸿文.黄瓜幼苗对热胁迫的生理反应及耐热鉴定指标筛选.西北农业学报,2000,9(1):96-99Meng H W,Zhang Y F,Cheng Z H,Su J,Cui H W.The physiological reaction to hot stress and screening of hot tolerance index in cucumber.Acta Agriculturae Borealioccidentalis Sinica,2000,9(1):96-99
[9]杨寅桂.黄瓜耐热性及热胁迫响应基因研究.南京:南京农业大学,2007Yang Y G.Study on the heat tolerance and gene of heat stress response related in cucumber.Nanjing:Nanjing Agricultural University,2007
[10]Qi J J,Liu X,Shen D,Miao H,Xie B Y,Li X X,Zeng P,Wang S H,Shang Y,Gu X F,Du Y C,Li Y,Lin T,Yuan J H,Yang X Y,Chen J F,Chen H M,Xiong X Y,Huang K,Fei ZJ,Mao L Y,Tian L,St?dler T,Renner S S,Kamoun S,Lucas W J,Zhang Z H,Huang S W.A genomic variation map provides insights into the genetic basis of cucumber domestication and diversity.Nature Genetics,2013,45(12):1510-1515
[11]苏世闻.黄瓜种子萌发和幼苗生长的耐热性差异及其产量和生理特性.南京:南京农业大学,2014Su S W.Yield and physiological characters of cucumber varieties and heat resistant difference on its seed germination and seedling growth.Nanjing:Nanjing Agricultural University,2014
[12]Ruan S,Xue Q,Thlkowska K.Effect of priming on germination and health of rice(Oryza sativa L.)seeds.Seed Science and Technology,2002,30(2):451-458
[13]Ruan S,Xue Q,Thlkowska K.The influence of priming on germination of rice(Oryza sativa L.)seeds and seedling emergence and performance in flooded soil.Seed Science and Technology,2002,30(1):61-67
[14]Esechie H.Interaction of salinity and temperature on the germination of sorghum.Journal of Agronomy and Crop Science,1994,172:194-199
[15]何延治.农业研究中常用的几种模糊数学方法.长春:吉林大学,2006He Y Z.Some usual kinds of methods of fuzzy mathematics in agriculture research.Changchun:Jilin University,2006
[16]谢志坚.农业科学中的模糊数学方法.武汉:华中理工大学出版社,1983:99-193Xie Z J.Fuzzy mathematics method in agricultural science.Wuhan:Huazhong University of Science and Technology Press,1983:99-193
[17]李青,秦玉芝,胡新喜,丁红映,熊兴耀,王万兴.马铃薯耐盐性离体鉴定方法的建立及52份种质资源耐盐性评价.植物遗传资源学报,2018,19(4):587-597Li Q,Qin Y Z,Hu X X,Ding H Y,Xiong X Y,Wang WX.Establishment of an optimized bioassay being valuable for determining salt tolerance in 152 potato germplasm accessions.Journal of Plant Genetic Resources,2018,19(4):587-597
[18]鲁宏伟.黄瓜白粉病抗性基因挖掘.北京:中国农业科学院,2015Lu H W.Mining of the resistance gene for powdery mildew in cucumber.Beijing:Chinese Academy of Agricultural Sciences,2015
[19]张朝阳,许桂芳.利用隶属函数法对4种地被植物的耐热性综合评价.草业科学,2009,26(2):57-60Zhang C Y,Xu G F.Comprehensive evaluation of heat tolerance of four ground cover plants by membership function method.Pratacultural Science,2009,26(2):57-60
[20]Romesburg H C.Cluster analysis for researchers.NC Raleigh.USA:Lulu.com,2004
[21]许端祥,高山,林碧英,傅睿清.高温胁迫下11份瓠瓜材料的耐热性分析.福建农业学报,2011,26(4):587-590Xu D X,Gao S,Lin B Y,Fu R Q.Heat resistance of 11Lagenaria siceraria var.hispida stand germplasms.Fujian Journal of Agricultural Sciences,2011,26(4):587-590
[22]吴欣明,郭璞,池惠武,方志红,石永红,王运琦,刘建宁,王赞,王学敏.国外紫花苜蓿种质资源表型性状与品质多样性分析.植物遗传资源学报,2018(1):103-111Wu X M,Guo P,Chi H W,Fang Z H,Shi Y H,Wang Y Q,Liu J N,Wang Z,Wang X M.Diversity analysis of phenotypic traits and quality characteristics of alfalfa(Medicago sativa)introducted from abroad Germplasm resources.Journal of Plant Genetic Resources,2018(1):103-111
[23]冯晶晶,刘磊,郑峥,邓学斌,刘希艳,白金瑞,舒金帅,宋燕,李君明.醋栗番茄Solanum pimpinellifolium遗传多样性分析.植物遗传资源学报,2017,18(4):611-619Feng J J,Liu L,Zheng Z,Deng X B,Liu X Y,Bai J R,Shu J S,Song Y,Li J M.Genetic diversity of wild tomato species Solanum pimpinellifolium.Journal of Plant Genetic Resources,2017,18(4):611-619
[24]吕婧.黄瓜种质资源群体结构分析与核心种质集筛选.北京:中国农业科学院,2011Lv J.Germplasm population structure analysis and core collection of cucumber(Cucumis sativus L.).Beijing:Chinese Academy of Agricultural Sciences,2011
[25]侯锋.黄瓜.天津:天津科学技术出版社,1999:90-91Hou F.Cucumber.Tianjin:Tianjin Science and Technology Press,1999:90-91
[26]张焕欣,翁建峰,张晓聪,刘昌林,雍洪军,郝转芳,李新海.玉米穗行数全基因组关联分析.作物学报,2014,40(1):1-6Zhang H X,Weng J F,Zhang X C,Liu C L,Yong H J,Hao Z F,Li X H.Genome-wide association analysis of kernel row number in maize.Acta Agronomica Sinica,2014,40(1):1-6
[27]Yu J M,Buckler E S.Genetic association mapping and genome organization of maize.Current Opinion in Biotechnology,2006,17(2):155-160
[28]Zhu C S,Gore,M,Buckler,E S,Yu J M.Status and prospects of association mapping in plants.Plant Genome,2008,1(1):5-20
[29]Huang X H,Zhao Y,Wei X H,Li C Y,Wang A H,Zhao Q,Li W J,Guo Y L,Deng L W,Zhu C R,Fan D L,Lu Y Q,Weng Q J,Liu K Y,Zhou T Y,Jing Y F,Si L Z,Dong G J,Huang T,Lu T T,Feng Q,Qian Q,Li J Y,Han B.Genome-wide association study of flowering time and grain yield traits in a worldwide collection of rice germplasm.Nature Genetics,2011,44(1):32
[30]Buckler E S,Holland J B,Bradbury P J,Acharya C B,Brown P J,Browne C,Ersoz E,Flint-Garcia S,Garcia A,Glaubitz J C,Goodman M M,Harjes C,Guill K,Kroon D E,Larsson S,Lepak N K,Li H,Mitchell S E,Pressoir G,Peiffer J A,Rosas M O,Rocheford T R,Romay M C,Romero S,Salvo S,Sanchez V H,da Silva H S,Sun Q,Tian F,Upadyayula N,Ware D,Yates H,Yu J,Zhang Z,Kresovich S,McMullen MD.The genetic architecture of maize flowering time.Science,2009,325(5941):714
[31]Kump K L,Bradbury P J,Wisser R J,Buckler E S,Belcher A R,Oropeza-Rosas M A,Zwonitzer J C,Kresovich S,McMullen M D,Ware D,Balint-Kurti P J,Holland J B.Genome-wide association study of quantitative resistance to southern leaf blight in the maize nested association mapping population.Nature Genetics,2011,43(2):163-168
[32]杨迪菲.黄瓜耐热性QTL定位的研究.哈尔滨:东北农业大学,2006Yang D F.Mapping quantitative traits loci for the heat tolerance in cucumber.Harbin:Northeast Agricultural University,2006
[33]庄影.黄瓜耐热性遗传及QTL初步定位.扬州:扬州大学,2014Zhuang Y.Genetic analysis and preliminary QTL mapping of heat tolerance in cucumber(Cucumis sativus L.).Yangzhou:Yangzhou University,2014