不育胺对橘小实蝇的化学不育效果
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摘要
【目的】为了解不育胺对橘小实蝇Bactrocera dorsalis(Hendel)的化学不育效果。【方法】通过喂食橘小实蝇不同浓度不育胺食料,测定了其对橘小实蝇的卵量及孵化率的影响。【结果】结果表明:喂食0.08%-0.3%不育胺食料的橘小实蝇成虫交配始见期和产卵始见期与正常对照相比推迟1-2d,均为9-10日龄开始交配,11-12日龄开始产卵;橘小实蝇取食不同浓度不育胺食料后,卵量和孵化率随浓度增加而降低。当取食0.3%不育胺食料时,♀处理×♂处理未见产卵,♀正常×♂处理和♀处理×♂正常均见产卵但卵量与对照相比显著降低且卵不孵化。取食0.3%不育胺的橘小实蝇雄虫相对不育系数为0.48,表明其交配竞争能力与正常雄虫相当。【结论】0.3%浓度的不育胺可作为橘小实蝇的化学不育剂。
[Objectives] To assess the sterilizing effect of metepa on Bactrocera dorsalis. [Methods] B. dorsalis were fed an artificial diet containing 0.08-0.3% metepa, and the effects of these different concentrations of metepa on egg production and hatching rate were recorded and compared. [Results] Feeding adults different concentrations of metepa delayed mating and oviposition by 1 to 2 days compared to the control group. All adults began mating at 9-10 days of age and began laying at 11-12 days of age. The number of eggs laid and the hatching rate decreased with increasing concentrations of metepa in the diet. A mixed group of females and males fed a diet containing 0.3% metepa failed to produce eggs. Sexually segregated females fed the same diet produced significantly fewer eggs than the control group and their eggs failed to hatch. The Relative Sterility Index(RST) of males fed a diet containing 0.3% metepa was 0.48, and metepa seemed to have no effect on male mating competitiveness. [Conclusion] A 0.3% concentration of metepa added to an artificial diet acts as a chemosterilant for B. dorsalis.
[Objectives] To assess the sterilizing effect of metepa on Bactrocera dorsalis. [Methods] B. dorsalis were fed an artificial diet containing 0.08-0.3% metepa, and the effects of these different concentrations of metepa on egg production and hatching rate were recorded and compared. [Results] Feeding adults different concentrations of metepa delayed mating and oviposition by 1 to 2 days compared to the control group. All adults began mating at 9-10 days of age and began laying at 11-12 days of age. The number of eggs laid and the hatching rate decreased with increasing concentrations of metepa in the diet. A mixed group of females and males fed a diet containing 0.3% metepa failed to produce eggs. Sexually segregated females fed the same diet produced significantly fewer eggs than the control group and their eggs failed to hatch. The Relative Sterility Index(RST) of males fed a diet containing 0.3% metepa was 0.48, and metepa seemed to have no effect on male mating competitiveness. [Conclusion] A 0.3% concentration of metepa added to an artificial diet acts as a chemosterilant for B. dorsalis.
引文
Abrecque GC,Meifert DW,Smirh CN,1962.Mating competitiveness of chemosterilized and normal male house flies.Journal of Science,(136):388-389.
Al-Adil KM,Kilgore WW,Painter RR,1972.House fly lipids and the effect of chemosterilization with metepa.Journal of Economic Entomology,65(3):887-888.
Chang CL,Cho K,Li QX,2012.Laboratory evaluation of the chemosterilant lufenuron against the fruit flies Ceratitis capitata,Bactrocera dorsalis,B.cucurbitae,and B.latifrons.Journal of Asia-Pacific Entomology,15(1):13-16.
Chen YC,Chen L,Shang HW,2013.Application of a mixture design to optimize the artincial diet for the oriental fruit fly,Bactrocera dorsalis.Chinese Joumal of Biological Control,29(3):354-361.[陈毅超,陈玲,商晗武,2013.混料设计在优化橘小实蝇人工饲料中的应用,29(3):170-173.]
Dai MX,Cheng GL,Du XL,Liu RX,2010.Study of Chemosterilization of Spodoptera liura Fabricius.Northern Horticulture,(4):170-173.[戴明勋,程桂林,杜学林,刘润玺,2010.斜纹夜蛾化学不育研究初报.北方园艺,(4):170-173.]
Guo QZ,1963.Insect chemosterilization.Bulletin of Biology,(6):19-20.[郭奇珍,1963.昆虫不育剂.生物学通报,(6):19-20.]
Haniotakis GE,Galachtiou CG,1973.Metepa sterilization of the olive fruit fly.Journal of Economic Entomology,66(1):55-61.
Huang SQ,Han RC,2005.Advance in the research on the quarantine pest Bactrocera dorsalis.Chinese Bulletin of Entomology,42(5):479-484.[黄素青,韩日畴,2005.桔小实蝇的研究进展.昆虫知识,42(5):479-484.]
Huang YY,Chen J,2006.The propagation characteristic of oriental fruit fly,Bactrocera dorsalis and control technique.EntomoIogicaI JournaI of East China,15(1):63-66.[黄月英,陈军,2006.橘小实蝇的发生特点与综合防治技术.华东昆虫学报,15(1):63-66.]
Ji QE,Hou WR,Chen JH,2007.Development of a genetic sexing strain and the sterile male technique of the Oriental fruit fly,Bactrocera dorsalis(Hendel)Acta Entomologica Sinica,50(10):1002-1008.[季清娥,侯伟荣,陈家骅,2007.橘小实蝇遗传性别品系的建立及雄性不育技术.昆虫学报,50(10):1002-1008.]
Ji QE,Hou WR,Chen JH,2007.Sterile insect technique of oriental fruit fly Bactrocera dorsalis(Hendel)-optimal pupal age and dose of irradiation treatment for male pupae.Journal of Nuclear Agricultural Sciences.21(5):523-526.[季清娥,侯伟荣,陈家骅,2007.橘小实蝇雄性不育技术-雄蛹辐照最佳时期和剂量.核农学报,21(5):523-526.]
Liang F,Liang GQ,Zhao JP,Zhou QX,Wu JJ,Kuang YW,Huang YH,Liu ZB,2014.Study status of attractants for fruit flies.Biological Disaster Science,37(3):204-210[梁帆,梁广勤,赵菊鹏,周庆贤,吴佳教,邝业文,黄跃辉,刘志斌,2014.实蝇引诱物的研究概况.生物灾害科学37(3):204-210.]
Liu RX,Chen GL,Jiang YC,Lv ZQ,Liu SW,Yu GL,Wang ZX,Du XL,Hu MJ,Dai MX,Que KF,Jiang SJ,Xiao B,1999.Study on the gossypol sterilant to control resistance cotton bollworm(Helicoverpa armigera).Pesticides,38(1):12-14.[刘润玺,程桂林,姜延超,吕昭芹,刘士文,于桂兰,王振孝,杜学林,胡明江,戴明勋,薛克富,姜士聚,肖斌,1999.棉酚不育剂防治抗性棉铃虫的研究.农药,38(1):12-14.]
Meng QQ,Zhang Z,Lin YY,Li SG,Lu YY,2012.Sterile effect of hexamethyl phosphoryl triamide on Bactrocera dorsalis Hendel adults.Guangdong Agricultural Sciences,39(4):62-64.[孟倩倩,章珍,林玉英,李世广,陆永跃,2012.六甲基磷酰三胺对桔小实蝇的不育效果研究.广东农业科学,39(4):62-64.]
Nair S,Thomas J,2001.Evaluation of the chemosterilant effect of Acorus calamus L.extracts on melon fly,Bactrocera cucurbitae Coq.Tropical Agriculture,39(2):145-148.
Teng B,1965.A preliminary observation on the mechanism of sterilization of the House-flies(Musca vicina Macquart)treated with Thio-Tepa.Acta Entomologica Sinica,14(3):250-254.[腾斌,1965.噻替派对家蝇不育机制的初步观察.昆虫学报,14(3):250-254.]
Tang H,Liu YN,Zhang J,1996.Hama’s carried dynamic state in the trunk of populous and its sterile effect on Anoplophora giabripennis.Journal of Shanxi Normal University(Natural Science Edition),24(2):72-76.[唐桦,刘益宁,张剑,1996.六磷胺在树干内的输导动态及其对光肩星天牛的不育效应.陕西师范大学学报(自然科学版),24(2):72-76.]
Vicente NL,Juan SC,IIdefonso A,Victor CG,Eduardo PY,2004.Efficacy of lufenuron as chemosterilant against Ceratitis capitata in field trials.Pest Manag.Sci.,60(9):914-920.
Vishal S,Sharma SK,2004.Induction of sterility in flesh fly,Sarcophaga Bravicornis by chemosterilization of pupae-Ⅱ.Jounery of Natcon,16(1):31-33.
Wang B,Huang JC,Ji QE,Yang JQ,Chen JH,2010.A study on the production of protein bait for Bactrocera dorsalis(Hendel).Acta Agricuhurae Universitatis Jiangxiensis,32(2):299-302.[王波,黄居昌,季清娥,杨建全,陈家骅,2010.酶解啤酒废酵母生产橘小实蝇蛋白饵剂的研究.江西农业大学学报,32(2):299-302.]
Yuan SY,Kong Q,Xue CL,Shen DR,Li X,Chen B,Qin WX,Zhang X,2015.The trapping effect of several fruit flavor on oriental fruit fly Bactrocera dorsalis(Hendel).South China Fruits,44(1):34-35.[袁盛勇,孔琼,薛春丽,沈登荣,李珣,陈斌,秦文旭,张霞,2015.几种果实香精诱集桔小实蝇成虫的效果.中国南树,44(1)34-35.]
Zheng HY,Liu JP,Nan HL,Liu WP,Mao LP,2000.Preliminary study on chemical sterility of cotton bollworm.Journal of Shanxi Agricultural Sciences,28(1):67-68.[郑洪源,刘建平,南怀林,刘文萍,毛丽萍,2000.棉铃虫化学不育试验初报.山西农业科学,28(1):65-67.]
Zhao JP,Liu LD,Lu YY,2017.Trapping efficacy of 4 kinds of imported attractants on oriental fruit fly Bactrocera dorsalis(Hendel).Journal of Environmental Entomology,39(4):830-834.[赵菊鹏,刘龙地,陆永跃,2017.4种引进的引诱剂制剂对桔小实蝇引诱效果评价.环境昆虫学报,39(4):830-834.]
Zhang QX,1989.The preliminary study on the mechanism of sterilization of the Culex fatigans treated with Hexamethyl phosphoryl triamide.Guangzhou Medical Journal,(2):47-48.[张全新,1989.六磷胺使致乏库蚊雄蚊不育机制的初步研究.广州医药,(2):47-48.]
Zhou SJ,Han MD,Li LX,Zhang XK,1980.The control effect on the Dendrolimus punctatus Walker treated with camptothecin and sex pheromone.Scientia Silvae Sinicae,16(3):234-235.[周石涓,韩明德,倪乐湘,张贤开,1980.性诱剂与植物不育剂-喜树碱结合应用防治松毛虫的效果初报.林业科学,16(3):234-235.]
Zou SF,Zhang YP,Cheng MY,Li DS,Zhang BX,2010.Influence of camptothecin to Bactrocera dorsalis Hendel adults.Guangdong Agricultural Sciences,37(2):89-92.[邹寿发,章玉苹,陈明洋,李敦松,张宝鑫,2010.喜树碱对桔小实蝇成虫影响的研究.广东农业科学,37(2):89-92.]
Zhao WC,Jiao HS,Zhang SF,1986.Studies on chemosterilant TH-6040 for armyworm moth.Acta Agriculturae BorealiSinica,1(2):111-114.[赵文臣,焦惠生,张淑芬,1986.灭幼脲一号致粘虫成虫产卵不育的研究-室内不育研究部分.华北农学报,1(2):111-114.]
Al-Adil KM,Kilgore WW,Painter RR,1972.House fly lipids and the effect of chemosterilization with metepa.Journal of Economic Entomology,65(3):887-888.
Chang CL,Cho K,Li QX,2012.Laboratory evaluation of the chemosterilant lufenuron against the fruit flies Ceratitis capitata,Bactrocera dorsalis,B.cucurbitae,and B.latifrons.Journal of Asia-Pacific Entomology,15(1):13-16.
Chen YC,Chen L,Shang HW,2013.Application of a mixture design to optimize the artincial diet for the oriental fruit fly,Bactrocera dorsalis.Chinese Joumal of Biological Control,29(3):354-361.[陈毅超,陈玲,商晗武,2013.混料设计在优化橘小实蝇人工饲料中的应用,29(3):170-173.]
Dai MX,Cheng GL,Du XL,Liu RX,2010.Study of Chemosterilization of Spodoptera liura Fabricius.Northern Horticulture,(4):170-173.[戴明勋,程桂林,杜学林,刘润玺,2010.斜纹夜蛾化学不育研究初报.北方园艺,(4):170-173.]
Guo QZ,1963.Insect chemosterilization.Bulletin of Biology,(6):19-20.[郭奇珍,1963.昆虫不育剂.生物学通报,(6):19-20.]
Haniotakis GE,Galachtiou CG,1973.Metepa sterilization of the olive fruit fly.Journal of Economic Entomology,66(1):55-61.
Huang SQ,Han RC,2005.Advance in the research on the quarantine pest Bactrocera dorsalis.Chinese Bulletin of Entomology,42(5):479-484.[黄素青,韩日畴,2005.桔小实蝇的研究进展.昆虫知识,42(5):479-484.]
Huang YY,Chen J,2006.The propagation characteristic of oriental fruit fly,Bactrocera dorsalis and control technique.EntomoIogicaI JournaI of East China,15(1):63-66.[黄月英,陈军,2006.橘小实蝇的发生特点与综合防治技术.华东昆虫学报,15(1):63-66.]
Ji QE,Hou WR,Chen JH,2007.Development of a genetic sexing strain and the sterile male technique of the Oriental fruit fly,Bactrocera dorsalis(Hendel)Acta Entomologica Sinica,50(10):1002-1008.[季清娥,侯伟荣,陈家骅,2007.橘小实蝇遗传性别品系的建立及雄性不育技术.昆虫学报,50(10):1002-1008.]
Ji QE,Hou WR,Chen JH,2007.Sterile insect technique of oriental fruit fly Bactrocera dorsalis(Hendel)-optimal pupal age and dose of irradiation treatment for male pupae.Journal of Nuclear Agricultural Sciences.21(5):523-526.[季清娥,侯伟荣,陈家骅,2007.橘小实蝇雄性不育技术-雄蛹辐照最佳时期和剂量.核农学报,21(5):523-526.]
Liang F,Liang GQ,Zhao JP,Zhou QX,Wu JJ,Kuang YW,Huang YH,Liu ZB,2014.Study status of attractants for fruit flies.Biological Disaster Science,37(3):204-210[梁帆,梁广勤,赵菊鹏,周庆贤,吴佳教,邝业文,黄跃辉,刘志斌,2014.实蝇引诱物的研究概况.生物灾害科学37(3):204-210.]
Liu RX,Chen GL,Jiang YC,Lv ZQ,Liu SW,Yu GL,Wang ZX,Du XL,Hu MJ,Dai MX,Que KF,Jiang SJ,Xiao B,1999.Study on the gossypol sterilant to control resistance cotton bollworm(Helicoverpa armigera).Pesticides,38(1):12-14.[刘润玺,程桂林,姜延超,吕昭芹,刘士文,于桂兰,王振孝,杜学林,胡明江,戴明勋,薛克富,姜士聚,肖斌,1999.棉酚不育剂防治抗性棉铃虫的研究.农药,38(1):12-14.]
Meng QQ,Zhang Z,Lin YY,Li SG,Lu YY,2012.Sterile effect of hexamethyl phosphoryl triamide on Bactrocera dorsalis Hendel adults.Guangdong Agricultural Sciences,39(4):62-64.[孟倩倩,章珍,林玉英,李世广,陆永跃,2012.六甲基磷酰三胺对桔小实蝇的不育效果研究.广东农业科学,39(4):62-64.]
Nair S,Thomas J,2001.Evaluation of the chemosterilant effect of Acorus calamus L.extracts on melon fly,Bactrocera cucurbitae Coq.Tropical Agriculture,39(2):145-148.
Teng B,1965.A preliminary observation on the mechanism of sterilization of the House-flies(Musca vicina Macquart)treated with Thio-Tepa.Acta Entomologica Sinica,14(3):250-254.[腾斌,1965.噻替派对家蝇不育机制的初步观察.昆虫学报,14(3):250-254.]
Tang H,Liu YN,Zhang J,1996.Hama’s carried dynamic state in the trunk of populous and its sterile effect on Anoplophora giabripennis.Journal of Shanxi Normal University(Natural Science Edition),24(2):72-76.[唐桦,刘益宁,张剑,1996.六磷胺在树干内的输导动态及其对光肩星天牛的不育效应.陕西师范大学学报(自然科学版),24(2):72-76.]
Vicente NL,Juan SC,IIdefonso A,Victor CG,Eduardo PY,2004.Efficacy of lufenuron as chemosterilant against Ceratitis capitata in field trials.Pest Manag.Sci.,60(9):914-920.
Vishal S,Sharma SK,2004.Induction of sterility in flesh fly,Sarcophaga Bravicornis by chemosterilization of pupae-Ⅱ.Jounery of Natcon,16(1):31-33.
Wang B,Huang JC,Ji QE,Yang JQ,Chen JH,2010.A study on the production of protein bait for Bactrocera dorsalis(Hendel).Acta Agricuhurae Universitatis Jiangxiensis,32(2):299-302.[王波,黄居昌,季清娥,杨建全,陈家骅,2010.酶解啤酒废酵母生产橘小实蝇蛋白饵剂的研究.江西农业大学学报,32(2):299-302.]
Yuan SY,Kong Q,Xue CL,Shen DR,Li X,Chen B,Qin WX,Zhang X,2015.The trapping effect of several fruit flavor on oriental fruit fly Bactrocera dorsalis(Hendel).South China Fruits,44(1):34-35.[袁盛勇,孔琼,薛春丽,沈登荣,李珣,陈斌,秦文旭,张霞,2015.几种果实香精诱集桔小实蝇成虫的效果.中国南树,44(1)34-35.]
Zheng HY,Liu JP,Nan HL,Liu WP,Mao LP,2000.Preliminary study on chemical sterility of cotton bollworm.Journal of Shanxi Agricultural Sciences,28(1):67-68.[郑洪源,刘建平,南怀林,刘文萍,毛丽萍,2000.棉铃虫化学不育试验初报.山西农业科学,28(1):65-67.]
Zhao JP,Liu LD,Lu YY,2017.Trapping efficacy of 4 kinds of imported attractants on oriental fruit fly Bactrocera dorsalis(Hendel).Journal of Environmental Entomology,39(4):830-834.[赵菊鹏,刘龙地,陆永跃,2017.4种引进的引诱剂制剂对桔小实蝇引诱效果评价.环境昆虫学报,39(4):830-834.]
Zhang QX,1989.The preliminary study on the mechanism of sterilization of the Culex fatigans treated with Hexamethyl phosphoryl triamide.Guangzhou Medical Journal,(2):47-48.[张全新,1989.六磷胺使致乏库蚊雄蚊不育机制的初步研究.广州医药,(2):47-48.]
Zhou SJ,Han MD,Li LX,Zhang XK,1980.The control effect on the Dendrolimus punctatus Walker treated with camptothecin and sex pheromone.Scientia Silvae Sinicae,16(3):234-235.[周石涓,韩明德,倪乐湘,张贤开,1980.性诱剂与植物不育剂-喜树碱结合应用防治松毛虫的效果初报.林业科学,16(3):234-235.]
Zou SF,Zhang YP,Cheng MY,Li DS,Zhang BX,2010.Influence of camptothecin to Bactrocera dorsalis Hendel adults.Guangdong Agricultural Sciences,37(2):89-92.[邹寿发,章玉苹,陈明洋,李敦松,张宝鑫,2010.喜树碱对桔小实蝇成虫影响的研究.广东农业科学,37(2):89-92.]
Zhao WC,Jiao HS,Zhang SF,1986.Studies on chemosterilant TH-6040 for armyworm moth.Acta Agriculturae BorealiSinica,1(2):111-114.[赵文臣,焦惠生,张淑芬,1986.灭幼脲一号致粘虫成虫产卵不育的研究-室内不育研究部分.华北农学报,1(2):111-114.]