超声辅助提取柴胡中柴胡皂苷a工艺响应面法优化研究
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摘要
目的:通过Box-Behnken响应面法优选柴胡中柴胡皂苷a的超声提取工艺。方法:采用超声提取法提取柴胡,以提取时间、超声功率、液料比作为考察因素,以柴胡皂苷a的提取率作为评价指标,采用Box-Behnken响应面法考察各个因素及其交互作用对柴胡皂苷a提取率的影响。结果:柴胡皂苷a超声提取的最佳工艺条件为:提取时间114 min,超声功率225 W,液料比40∶1(mL/g)。采用最佳提取工艺参数,平行提取柴胡3份,柴胡皂苷a的平均提取量为(11.19±0.5)mg/g(n=3),与预测值11.33 mg/g相比,RSD <5%。结论:响应面法优选的柴胡皂苷a超声提取工艺稳定可行,预测性良好。
Objective:To optimize the ultrasonic extraction process of saikosapoin a from Bupleurum Chinense DC. by Box-Behnken response surface methodology. Methods:Ultrasonic extraction method was used to extract Bupleurum Chinense DC.. The extraction time,ultrasonic power and methanol content was taken as the influencing factors. And the extraction rate of saikosapoin a was taken as the taken as the evaluation index. The effects of three processing variables and their interactions on the extraction rate were studied by Box-Behnken response surface methodology. Results:The results indicated that the optimal extraction conditions were as follows:the extraction time was 114 minutes;the ultrasonic power was 225 W;the methanol to raw material ratio was 40∶1(mL/g). Under the above conditions,3 batches of Bupleurum Chinense DC. were extracted. The average extraction rate of saikosapoin a was(11.19 ± 0.5)mg/g(n=3). RSD was less than 5%,compared with the predicted value of 11.33 mg/g. Conclusion:The extraction process of Bupleurum Chinense DC. optimized by Box-Behnken response surface methodology is stable,feasible,and predictable.
Objective:To optimize the ultrasonic extraction process of saikosapoin a from Bupleurum Chinense DC. by Box-Behnken response surface methodology. Methods:Ultrasonic extraction method was used to extract Bupleurum Chinense DC.. The extraction time,ultrasonic power and methanol content was taken as the influencing factors. And the extraction rate of saikosapoin a was taken as the taken as the evaluation index. The effects of three processing variables and their interactions on the extraction rate were studied by Box-Behnken response surface methodology. Results:The results indicated that the optimal extraction conditions were as follows:the extraction time was 114 minutes;the ultrasonic power was 225 W;the methanol to raw material ratio was 40∶1(mL/g). Under the above conditions,3 batches of Bupleurum Chinense DC. were extracted. The average extraction rate of saikosapoin a was(11.19 ± 0.5)mg/g(n=3). RSD was less than 5%,compared with the predicted value of 11.33 mg/g. Conclusion:The extraction process of Bupleurum Chinense DC. optimized by Box-Behnken response surface methodology is stable,feasible,and predictable.
引文
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[11]王玲丽,藤红梅,郭艳茹,等.响应面分析法优化超声提取细叶远志皂苷工艺研究[J].中药材,2014,37(4):679-683.
[12]张小飞,果秋婷. Box-Behnken响应面法优化杜仲炮制工艺[J].辽宁中医药大学学报,2018,20(4):68-71.
[13]朱晓伟,陈建平,布仁,等.超声提取蒙药漏芦花中总皂苷工艺研究[J].中南药学,2013,11(11):812-814.
[14]果秋婷,张小飞. Box-Behnken响应面法优化三黄地榆中盐酸小檗碱的提取工艺[J].中国药师,2017,20(1):56-59.
[15]王志花,金成国,刘义博,等.柴胡中柴胡皂苷a的超声辅助溶剂提取[J].分析实验室,2007,26(3):106-109.
[2]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:280-281.
[3]赵香妍,刘长利.中药柴胡的研究概况与发展趋势[J].时珍国医国药,2015,26(4):963-966.
[4]黄伟,孙蓉.柴胡皂苷类成分化学与药理和毒理作用研究进展[J].中药药理与临床,2010,26(3):71-74.
[5]Takai K,Shibenito PB,Abad MJ,et al. In vivo and in vitro antiflammatory activity of saikosaponins[J]. Life sci,1998,63(13):1147-1156.
[6]谢东浩,蔡宝昌,安益强,等.柴胡皂苷类化学成分及药理作用研究进展[J].南京中医药大学学报,2007,23(1):63-65.
[7]刘耕陶.中药药理研究与药物创新[M].北京:中国协和医科大学出版社,2006:75-76.
[8]肖功胜,杨云,刘富岗,等.柴胡皂苷提取和含量测定方法研究[J].中成药,2004,31(4):595-598.
[9]孙印石,王建华,朱玉霞,等.响应面法优化超声提取柴胡皂苷a、c、d的工艺研究[J].中药材,2011,34(11):1776-1780.
[10]国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:59-61.
[11]王玲丽,藤红梅,郭艳茹,等.响应面分析法优化超声提取细叶远志皂苷工艺研究[J].中药材,2014,37(4):679-683.
[12]张小飞,果秋婷. Box-Behnken响应面法优化杜仲炮制工艺[J].辽宁中医药大学学报,2018,20(4):68-71.
[13]朱晓伟,陈建平,布仁,等.超声提取蒙药漏芦花中总皂苷工艺研究[J].中南药学,2013,11(11):812-814.
[14]果秋婷,张小飞. Box-Behnken响应面法优化三黄地榆中盐酸小檗碱的提取工艺[J].中国药师,2017,20(1):56-59.
[15]王志花,金成国,刘义博,等.柴胡中柴胡皂苷a的超声辅助溶剂提取[J].分析实验室,2007,26(3):106-109.