大孔吸附树脂纯化细梗香草总皂苷的工艺研究
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摘要
筛选纯化细梗香草总皂苷的最佳大孔树脂,采用单因素试验和正交设计试验优化细梗香草总皂苷的大孔吸附树脂纯化工艺。结果表明,DM130型大孔树脂对细梗香草总皂苷的吸附性能良好,优化后的纯化工艺条件为:上样药液浓度0. 25 g生药·mL~(-1)(p H=8),上样流速4 BV·h~(-1),吸附0. 5 h,依次用蒸馏水、40%乙醇、70%乙醇洗脱4 BV,洗脱流速4 BV·h~(-1)。在此条件下,细梗香草总皂苷的洗脱率达92. 03%,纯度50. 37%。
To select the best macroporous resin and study the purification of total saponins from Lysimachia capillipes Hemsl.,the optimal purification condition were investigated by single factor test and orthogonal design test. The results showed that DM 130 macroporous resin presented good capabilities of adsorption and desorption of total saponins from Lysimachia capillipes Hemsl.,and the optimum condition of DM130 was as follows: the sample concentration was 0. 25,the sample flow rate was 4 BV·h~(-1),the adsorption was 0. 5 h,The optimal elution conditions were eluted by distilled water,40% ethanol and 70% ethanol at a flow rate of 4 BV·h~(-1),and the elution volume was 4 BV. With the optimum technological process,the elution ratio of total saponins was 92. 03% and the purity reached 50. 37%.
To select the best macroporous resin and study the purification of total saponins from Lysimachia capillipes Hemsl.,the optimal purification condition were investigated by single factor test and orthogonal design test. The results showed that DM 130 macroporous resin presented good capabilities of adsorption and desorption of total saponins from Lysimachia capillipes Hemsl.,and the optimum condition of DM130 was as follows: the sample concentration was 0. 25,the sample flow rate was 4 BV·h~(-1),the adsorption was 0. 5 h,The optimal elution conditions were eluted by distilled water,40% ethanol and 70% ethanol at a flow rate of 4 BV·h~(-1),and the elution volume was 4 BV. With the optimum technological process,the elution ratio of total saponins was 92. 03% and the purity reached 50. 37%.
引文
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[4]徐翔英,谢秋情,郑冬梅,等.星点设计-响应面法优选细梗香草总皂苷自微乳处方[J].中国药学杂志,2017,52(22):2012-2017.
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[6]明惠仪,王篧,麻秀萍,等.白英总皂苷纯化工艺研究[J].亚太传统医药,2018,14(5):36-38.
[7]宋艺君,郭涛,王昌利.穿山龙总皂苷大孔吸附树脂纯化工艺研究[J].中医药信息,2015,32(1):3-6.
[8]李嘉滢.酸枣仁总皂苷的提取分离纯化研究[D].湖南中医药大学,2014.
[9]韩伟,刘曦,等.枇杷叶三萜酸的大孔树脂分离纯化工艺[J].南京工业大学学报(自科版),2014,36(6):66-71.
[10]孔辉,郑德,周洪雷,等.远志总皂苷大孔吸附树脂纯化工艺研究[J].辽宁中医杂志,2014(10):2196-2199.
[2]花永虹,胡巧英,朴永锋,等.细梗香草皂甙对鼻咽癌CNE-2细胞的体外抗瘤作用[J].中国肿瘤,2014,23(7):597-600.
[3]程忠哲.细梗香草两个主要抗肿瘤活性皂苷成分的药代动力学研究[D].武汉:华中科技大学,2015.
[4]徐翔英,谢秋情,郑冬梅,等.星点设计-响应面法优选细梗香草总皂苷自微乳处方[J].中国药学杂志,2017,52(22):2012-2017.
[5]黄鹤洁.大孔吸附树脂在分离提取中药皂苷有效部位中的应用[J].亚太传统医药,2009,5(6):41-43.
[6]明惠仪,王篧,麻秀萍,等.白英总皂苷纯化工艺研究[J].亚太传统医药,2018,14(5):36-38.
[7]宋艺君,郭涛,王昌利.穿山龙总皂苷大孔吸附树脂纯化工艺研究[J].中医药信息,2015,32(1):3-6.
[8]李嘉滢.酸枣仁总皂苷的提取分离纯化研究[D].湖南中医药大学,2014.
[9]韩伟,刘曦,等.枇杷叶三萜酸的大孔树脂分离纯化工艺[J].南京工业大学学报(自科版),2014,36(6):66-71.
[10]孔辉,郑德,周洪雷,等.远志总皂苷大孔吸附树脂纯化工艺研究[J].辽宁中医杂志,2014(10):2196-2199.