石墨炉标准加入校正法快速测定全血中的镉
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摘要
目的:建立石墨炉原子吸收标准加入校正法快速测定全血中镉的方法。方法:取血样0.10 ml,用1 ml/L硝酸和0.1 g/L Triton X-100作为稀释液直接稀释10倍,0.2 g/L氯化钯作基体改进剂,塞曼扣除背景,峰面积定量,石墨炉原子吸收标准加入校正法直接自动进样测定。结果:方法的线性范围为0μg/L～3.0μg/L,线性相关系数r=0.9991,方法的最低检出限为0.081μg/L,相对标准偏差(RSD)为1.8%～7.2%,加标回收率为95.0%～106.0%,应用本法测定冻干牛血铅、镉标准物质GBW09139e、GBW09140e结果与标准值相吻合。结论:本法准确度、精密度良好,样品不需消化,无需正常人血配置标准系列,自动化程度高,是一种简便、快速、可行的全血镉检测方法。
Objective:To develop a rapid method for determination of blood Cadmium by graphite furnace and standard addition method.Methods:0.10 ml blood sample was directly diluted to 10 times with 1 ml/L HNO3 and 0.1 g/L Triton X-100,and then determined by graphite furnace atomic absorption standard addition method via direct automatic injection,with 0.2 g/L Pdcl2 as matrix modifier,Zeeman effect for correcting disturbance of background and peak area for quantification.Results:There was significant linear relation between absorbance and Cadmium level in the range of 0 μg/L~3.0 μg/L.The correlation coefficient was 0.9991,the lowest detection limit was 0.081 μg/L,the recovery of standard addition was during 95.0%~106.0%,the RSD of parallel double sample was 1.8%~7.2%.The method was successfully applied to determine Certified Reference Materials such as GBW09139e and GBW09140e,and the results were in good agreement with the certified values.Conclusion: The measurement is characterized by high accuracy and precision,no need for chemical treatment,no need to use standard series with normal human blood.This method is good for determination of blood Cadmium simply and rapidly.
Objective:To develop a rapid method for determination of blood Cadmium by graphite furnace and standard addition method.Methods:0.10 ml blood sample was directly diluted to 10 times with 1 ml/L HNO3 and 0.1 g/L Triton X-100,and then determined by graphite furnace atomic absorption standard addition method via direct automatic injection,with 0.2 g/L Pdcl2 as matrix modifier,Zeeman effect for correcting disturbance of background and peak area for quantification.Results:There was significant linear relation between absorbance and Cadmium level in the range of 0 μg/L~3.0 μg/L.The correlation coefficient was 0.9991,the lowest detection limit was 0.081 μg/L,the recovery of standard addition was during 95.0%~106.0%,the RSD of parallel double sample was 1.8%~7.2%.The method was successfully applied to determine Certified Reference Materials such as GBW09139e and GBW09140e,and the results were in good agreement with the certified values.Conclusion: The measurement is characterized by high accuracy and precision,no need for chemical treatment,no need to use standard series with normal human blood.This method is good for determination of blood Cadmium simply and rapidly.
引文
[1]WS/T174-1999.血中铅、镉的石墨炉原子吸收光谱测定方法[S].
[2]勾艳玲,张琦.全血中镉石墨炉原子吸收法测定[J].中国公共卫生,2011,27(3):383.
[3]李凭建.全血中镉的无焰原子吸收光谱测定法[J].中华劳动卫生职业病杂志,2001,19(5):387-388.
[4]易春林,李文典.基于动物血液中铅、镉含量的原子吸收法测定研究[J].中国卫生检验杂志,2008,18(7):1447-1448.
[5]张颖,仲立新.平台石墨炉直接测定全血中镉[J].中国卫生检验杂志,2010,20(7):1910-1912.
[2]勾艳玲,张琦.全血中镉石墨炉原子吸收法测定[J].中国公共卫生,2011,27(3):383.
[3]李凭建.全血中镉的无焰原子吸收光谱测定法[J].中华劳动卫生职业病杂志,2001,19(5):387-388.
[4]易春林,李文典.基于动物血液中铅、镉含量的原子吸收法测定研究[J].中国卫生检验杂志,2008,18(7):1447-1448.
[5]张颖,仲立新.平台石墨炉直接测定全血中镉[J].中国卫生检验杂志,2010,20(7):1910-1912.