外源物质对盐胁迫下金莲花幼苗生长及生理特性的影响
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摘要
以金莲花幼苗为试验材料,采用硝普钠(SNP)、亚精胺(Spd)和水杨酸(SA) 3种不同浓度的外源物质对盐胁迫条件下金莲花幼苗进行缓解试验,探索最佳的外源物质及其浓度。结果表明,金莲花幼苗随着不同浓度SA、SNP和Spd对金莲花幼苗进行恢复处理后各指标均显著升高。由此可知,SA、SNP、Spd在合适的浓度下可以缓解NaCl对金莲花的胁迫伤害,增强金莲花的抗盐胁迫能力,其中最佳处理组合是20%的水杨酸(SA),此组合的各项指标均与空白对照相差无几。本研究为金莲花幼苗在实际生产中减缓盐胁迫伤害提供可行的途径,并且可以为进一步研究缓解盐胁迫提供依据。
The seedlings of Trollius chinensis were selected as the material, and different concentrations of sodium nitrate(SNP), alimine(Spd) and salicylic acid(SA) were used as exogenous substances to carry out remission experiment on the seedlings of Trollius chinensis under drought stress, so as to explore the optimal exogenous substance and its concentration. The results showed that the recovery treatment of different concentrations of SA, SNP and Spd on the seedlings of Trollius chinensis significantly increased the indexes of the seedlings of Trollius chinensis. It could be seen that the appropriate concentration of SA, SNP and Spd could alleviate the stress injury of NaCl to Trollius chinensis, and improve the tolerance of Trollius chinensis to salt. Among them, the optimal effect was SA with the concentration of 20%. The indexes of Trollius chinensis after this treatment had no significant difference with the control. This study could provide feasible ways for alleviating the injury of salt stress on Trollius chinensis in the practical production, and provide reference for further study on the relief of salt stress.
The seedlings of Trollius chinensis were selected as the material, and different concentrations of sodium nitrate(SNP), alimine(Spd) and salicylic acid(SA) were used as exogenous substances to carry out remission experiment on the seedlings of Trollius chinensis under drought stress, so as to explore the optimal exogenous substance and its concentration. The results showed that the recovery treatment of different concentrations of SA, SNP and Spd on the seedlings of Trollius chinensis significantly increased the indexes of the seedlings of Trollius chinensis. It could be seen that the appropriate concentration of SA, SNP and Spd could alleviate the stress injury of NaCl to Trollius chinensis, and improve the tolerance of Trollius chinensis to salt. Among them, the optimal effect was SA with the concentration of 20%. The indexes of Trollius chinensis after this treatment had no significant difference with the control. This study could provide feasible ways for alleviating the injury of salt stress on Trollius chinensis in the practical production, and provide reference for further study on the relief of salt stress.
引文
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Jin Z.M.,Sha W.,Sun W.,and Chai H.,2010,Effects of drought stress on protective enzymes of white clover seedlings,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),38(7):52-53(金忠民,沙伟,臧威,孙微,柴华,2010,干旱胁迫对白三叶幼苗保护酶的影响,东北林业大学学报,38(7):52-53)
Sang Z.Y.,Ma L.Y.,and Chen F.J.,2011,Growth and physiological characteristics of Magnoliawuf engensis seedlings under drought stress,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),31(1):109-115(桑子阳,马履一,陈发菊,2011,干旱胁迫对红花玉兰幼苗生长和生理特性的影响,西北植物学报,31(1):109-115)
Wang Y.P.,Dong W.,Zhang X.,Yang Q.,and Zhang F.,2012,Effects of salicylic acid on seeds germination and seedling physiological characteristics of cauliflower under salt stress,Caoye Xuebao(Acta Prataculturae Sinica),21(1):213-219(王玉萍,董雯,张鑫,杨茜,张峰,2012,水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响,草业学报,21(1):213-219)
Wang Z.J.,Wang J.,Jiao X.Y.,Chen Y.S.,Xu Z.D.,Ou Z.L.,Wu X.,and He Z.A.,2016 Effects of salt stress and exogenous calcium on seed germination of Rhus chinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(3):706-714(王占军,王静,焦小雨,陈延松,徐忠东,欧祖兰,吴席,何子昂,2016,盐胁迫及外源钙处理对盐肤木种子萌发的影响,基因组学与应用生物学,36(3):706-714)
Wang Z.L.,and Wang Z.W.,2016,Research advance and prospect of greenhouse soil degradation,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),44(18):95-98(王子璐,王祖伟,2016,设施土壤退化研究进展与展望,安徽农业科学,44(18):95-98)
Yang J.S.,2008,Development and prospect of the research on salt-affected soils in China,Turang Xuebao(Acta Pedologica Sinica),45(5):837-845(杨劲松,2008,中国盐渍土研究的发展历程与展望,土壤学报,45(5):837-845)
Yi X.B.,LQ.,and Wang S.W.,2013,Research progress of exogenous substances for alleviate seed germination under salt stress,Heilongjiang Nongye Kexue(Heilongjiang Agricul tural Sciences),(11):147-150(尹相博,李青,王绍武,2013,外源物质缓解盐胁迫下植物幼苗生长的研究进展,黑龙江农业科学,(11):147-150)
Zhang C.P.,He P.,Yu Z.L.,Du D.D.,and Hu S.J.,2011,Effects of spermidine on seed germination and physiological characteristics of Perilla frutescens seedlings under NaCl stress,Zhongcaoyao(Chinese Traditional and Herbal Drugs),42(7):1407-1412(张春平,何平,喻泽莉,杜丹丹,胡世俊,2011,亚精胺对盐胁迫下紫苏种子萌发和幼苗生理特性的影响,中草药,42(7):1407-1412)
Chen D.M.,Yu R.P.,and Yang J.S.,2002,Evaluation of salt resistance of wheat with subordinate function value method,Turang Xuebao(Acta Pedologica Sinica),39(3):368-374(陈德明,俞仁培,杨劲松,2002,盐渍条件下小麦抗盐性的隶属函数值法评价,土壤学报,39(3):368-374)
He S.X.,Liang Z.S.,Wei L.Z.,and Zhou Z.Y.,2009,Effects of soil drought on growth and physiological characteristics of wild jujube seedlings of two provenances,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),29(7):1387-1393(贺少轩,梁宗锁,蔚丽珍,周自云,2009,土壤干旱对2个种源野生酸枣幼苗生长和生理特性的影响,西北植物学报,29(7):1387-1393)
Jin Z.M.,Sha W.,Sun W.,and Chai H.,2010,Effects of drought stress on protective enzymes of white clover seedlings,Dongbei Linye Daxue Xuebao(Journal of Northeast Forestry University),38(7):52-53(金忠民,沙伟,臧威,孙微,柴华,2010,干旱胁迫对白三叶幼苗保护酶的影响,东北林业大学学报,38(7):52-53)
Sang Z.Y.,Ma L.Y.,and Chen F.J.,2011,Growth and physiological characteristics of Magnoliawuf engensis seedlings under drought stress,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),31(1):109-115(桑子阳,马履一,陈发菊,2011,干旱胁迫对红花玉兰幼苗生长和生理特性的影响,西北植物学报,31(1):109-115)
Wang Y.P.,Dong W.,Zhang X.,Yang Q.,and Zhang F.,2012,Effects of salicylic acid on seeds germination and seedling physiological characteristics of cauliflower under salt stress,Caoye Xuebao(Acta Prataculturae Sinica),21(1):213-219(王玉萍,董雯,张鑫,杨茜,张峰,2012,水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响,草业学报,21(1):213-219)
Wang Z.J.,Wang J.,Jiao X.Y.,Chen Y.S.,Xu Z.D.,Ou Z.L.,Wu X.,and He Z.A.,2016 Effects of salt stress and exogenous calcium on seed germination of Rhus chinensis,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),36(3):706-714(王占军,王静,焦小雨,陈延松,徐忠东,欧祖兰,吴席,何子昂,2016,盐胁迫及外源钙处理对盐肤木种子萌发的影响,基因组学与应用生物学,36(3):706-714)
Wang Z.L.,and Wang Z.W.,2016,Research advance and prospect of greenhouse soil degradation,Anhui Nongye Kexue(Journal of Anhui Agricultural Sciences),44(18):95-98(王子璐,王祖伟,2016,设施土壤退化研究进展与展望,安徽农业科学,44(18):95-98)
Yang J.S.,2008,Development and prospect of the research on salt-affected soils in China,Turang Xuebao(Acta Pedologica Sinica),45(5):837-845(杨劲松,2008,中国盐渍土研究的发展历程与展望,土壤学报,45(5):837-845)
Yi X.B.,LQ.,and Wang S.W.,2013,Research progress of exogenous substances for alleviate seed germination under salt stress,Heilongjiang Nongye Kexue(Heilongjiang Agricul tural Sciences),(11):147-150(尹相博,李青,王绍武,2013,外源物质缓解盐胁迫下植物幼苗生长的研究进展,黑龙江农业科学,(11):147-150)
Zhang C.P.,He P.,Yu Z.L.,Du D.D.,and Hu S.J.,2011,Effects of spermidine on seed germination and physiological characteristics of Perilla frutescens seedlings under NaCl stress,Zhongcaoyao(Chinese Traditional and Herbal Drugs),42(7):1407-1412(张春平,何平,喻泽莉,杜丹丹,胡世俊,2011,亚精胺对盐胁迫下紫苏种子萌发和幼苗生理特性的影响,中草药,42(7):1407-1412)