主成分分析下磐安浙贝母鳞茎最佳采收期的研究
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摘要
[目的]测定磐安浙贝母鳞茎主成分的含量,确定浙产浙贝母最佳采收期。[方法]分别采用HPLC-ELSD、酸性染料比色法、生物量法测定磐安地区浙贝母不同生长期鳞茎中贝母素甲、贝母素乙、总生物碱和生物量。[结果]磐安地区浙贝母鳞茎总生物碱含量在3月底、4月初最低,在5月初达到峰值,而鳞茎贝母素甲和贝母素乙含量变化规律则与其相反。结合生物量后,浙贝母鳞茎主成分含量在4月底5月初达到最高值。[结论]结合浙贝母鳞茎生物量、地上部分的生物学特性进行综合判断,磐安地区浙贝母最佳采收期在其果期末期、枯萎初期。
[Objective] Dynamic changes of biomass and alkaloids of Fritilarise thunbergii Bulbus were determined to assess the optimal collection time.[Methods] The contents of total alkaloids in the bulbs of Fritillaria thunbergii at different growth stages were determined by HPLC-ELSD, acid dye colorimetric method and biomass method. [Results] The total alkaloid content of Fritillaria thunbergii bulb in Pan'an in late March and early April the lowest peak in early May, and the bulb of peimine and peiminine in the opposite. Combined with biomass, the main components in bulbs of Fritillaria thunbergii reached the highest value at the end of April and early May. [Conclusion] Combined with the biomass in bulbs of Fritillaria thunbergii and the biological characteristics of the ground part of the comprehensive judgment, we found that the best harvest period of Fritilarise thunbergii. was between the end stage of its fruit stage and the early stage of withering stage.
[Objective] Dynamic changes of biomass and alkaloids of Fritilarise thunbergii Bulbus were determined to assess the optimal collection time.[Methods] The contents of total alkaloids in the bulbs of Fritillaria thunbergii at different growth stages were determined by HPLC-ELSD, acid dye colorimetric method and biomass method. [Results] The total alkaloid content of Fritillaria thunbergii bulb in Pan'an in late March and early April the lowest peak in early May, and the bulb of peimine and peiminine in the opposite. Combined with biomass, the main components in bulbs of Fritillaria thunbergii reached the highest value at the end of April and early May. [Conclusion] Combined with the biomass in bulbs of Fritillaria thunbergii and the biological characteristics of the ground part of the comprehensive judgment, we found that the best harvest period of Fritilarise thunbergii. was between the end stage of its fruit stage and the early stage of withering stage.
引文
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[9]郑坚,陶正明,李林,等.不同产区浙贝母种(鳞)茎环境因子及产量研究[J].浙江农业科学,2005,35(5):356-358.ZHENG Jian,TAO Zhengming,LI Lin,et al.Studies on environmental factors and yield of Fritillaria thunbergii seedling in different areas[J].Zhejiang Agricultural Sciences,2005,35(5):356-358.
[2]卓诗勤,张浩,丁弋娜,等.硫熏和鲜切浙贝母的化学成分及其药理作用的比较研究[J].中华中医药学刊,2016,34(3):618-621.ZHUO Shiqin,ZHANG Hao,DING Yina,et al.A Comparative Study on the Chemical Constituents and Pharmacological Effects of Sulfur Fumed and Fresh Fritillaria thunbergii[J].Journal of Chinese Medicine,2016,34(3):618-621.
[3]乔立俊.延胡索有效成分提取工艺研究[J].中药制剂工程与技术,2009,10(6):23.QIAO Lijun.Study on Extraction Technology of Active Components from Corydalis yanhusuo[J].Traditional Chinese Medicine Preparation Engineering and Technology,2009,10(6):23.
[4]徐金中,张红叶,马喜彦,等.浙江主产区栽培浙贝母种质遗传多样性的AFLP分析[J].中草药,2010,41(1):109-110.XU Jinzhong,ZHANG Hongye,MA Xiyan,et al.Genetic diversity of Fritillaria thunbergii cultivated in main producing areas of Zhejiang Province by AFLP analysis[J].Chinese Traditional and Herbal Drugs,2010,41(1):109-110.
[5]徐晓婷,田婧,林宏英,等.浙贝母总生物碱提取工艺优化研究[J].世界科学技术(中医药现代化),2012,82(6):2231-2234.XU Xiaoting,TIAN Jing,LIN Hongying,et al.Study on Extraction Technology of Total Alkaloids from Fritillaria thunbergii[J].World Science and Technology(Modernization of Traditional Chinese Medicine),2012,82(6):2231-2234.
[6]王艳红,吴晓民,郑友兰.不同产地和采收期的平贝母总生物碱含量[J].中药材,2006,29(1):8-9.WANG Yanhong,WU Xiaomin,ZHENG Youlan.al.The total alkaloid content of Fritillaria ussuriensis from different producing areas and harvest time[J].Chinese Traditional and Medicinal Materials,2006,29(1):8-9.
[7]李松林,李萍,卞芸芸,等.浙贝母总生物的月积累动态的研究[J].中国中药杂志,1999,24(10):592-593.LI Songlin,LI Ping,BIAN Yunyun,et al.Study on the Monthly Accumulation Dynamics of Fritillaria thunbergii[J].Chinese Journal of Traditional Chinese Medicine,1999,24(10):592-593.
[8]王和平,高莉莉,董珂.浙贝母不同采集时间及加工方法的研究[J].中医药学报,2007,35(6):35-36.WANG Heping,GAO Lili,DONG Ke.Study on different acquisition time and processing method of Fritillaria thun bergii[J].Chinese Journal of Traditional Chinese Medicine,2007,35(6):35-36.
[9]郑坚,陶正明,李林,等.不同产区浙贝母种(鳞)茎环境因子及产量研究[J].浙江农业科学,2005,35(5):356-358.ZHENG Jian,TAO Zhengming,LI Lin,et al.Studies on environmental factors and yield of Fritillaria thunbergii seedling in different areas[J].Zhejiang Agricultural Sciences,2005,35(5):356-358.