牛筋草对百草枯、草甘膦和草铵膦的抗药性水平测定
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摘要
【目的】本文通过比较分析牛筋草对百草枯、草甘膦和草铵膦的抗药性指数,旨在筛选出对3种除草剂产生多抗性的牛筋草种群,为杂草抗药性机制的深入研究及其合理治理奠定基础。【方法】以采集于广东省不同地区的13个牛筋草种群为材料,采用整株生物测定法对牛筋草种群进行百草枯和草铵膦抗药性水平测定,并采用培养皿法和莽草酸含量法对牛筋草种群进行草甘膦抗药性水平测定。【结果】(1)采集的13个样品对这3种除草剂均存在不同程度的抗药性,其中CZ、PY06、QY01、QY05、MZ02和NX种群存在对百草枯和草甘膦产生多抗性的可能;(2)CZ和PY06种群存在对草甘膦和草铵膦产生多抗性的可能;(3)CZ、PY06和ZQ02种群存在对百草枯和草铵膦产生多抗性的可能;(4)CZ和PY06种群存在对百草枯、草甘膦和草铵膦产生多抗性的可能。【结论】发现了对百草枯、草甘膦和草铵膦均产生抗药性的牛筋草种群。
【Objective】This study was to select out the multiple resistance Eleusine indica populations to paraquat,glyphosate and glufosinate by investigating the resistance level of E. indica to the three kinds of herbicides,laying a foundation for the research of weed resistance mechanism and the reasonable management.【Method】The resistance level of 13 E. indica populations in different areas of Guangdong province were investigated by using the method of whole-plant bioassay for paraquat and glufosinate,seed bioassay and shikimic acid content for glyphosate. 【Result】( i) CZ,PY06,QY01,QY05,MZ02 and NX populations had the potential of multiple resistance to paraquat and glyphosate;( ii) CZ and PY06 populations had the potential of multiple resistance to glyphosate and glufosinate;( iii) CZ,PY06 and ZQ02 populations had the potential of multiple resistance to paraquat and glufosinate;( iv) However,CZ and PY06 populations had the potential of multiple resistance to paraquat,glyphosate and glufosinate.【Conclution】It was found that the Eleusine indica populations had the potential of multiple resistance to paraquat,glyphosate and glufosinate.
【Objective】This study was to select out the multiple resistance Eleusine indica populations to paraquat,glyphosate and glufosinate by investigating the resistance level of E. indica to the three kinds of herbicides,laying a foundation for the research of weed resistance mechanism and the reasonable management.【Method】The resistance level of 13 E. indica populations in different areas of Guangdong province were investigated by using the method of whole-plant bioassay for paraquat and glufosinate,seed bioassay and shikimic acid content for glyphosate. 【Result】( i) CZ,PY06,QY01,QY05,MZ02 and NX populations had the potential of multiple resistance to paraquat and glyphosate;( ii) CZ and PY06 populations had the potential of multiple resistance to glyphosate and glufosinate;( iii) CZ,PY06 and ZQ02 populations had the potential of multiple resistance to paraquat and glufosinate;( iv) However,CZ and PY06 populations had the potential of multiple resistance to paraquat,glyphosate and glufosinate.【Conclution】It was found that the Eleusine indica populations had the potential of multiple resistance to paraquat,glyphosate and glufosinate.
引文
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[3]Alfen N K V,Preston C.Plant Biotic Stress:Weeds[M].Encyclopedia of Agriculture and Food Systems,Oxford:Academic Press,2014:343-348.
[4]An J,Shen X F,Ma Q B,et al.Transcriptome profiling to discover putative genes associated with paraquat resistance in goosegrass(Eleusine indica L.)[J].Plos One,2014,9(6):e99940.
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[9]Heap I.International survey of herbicide resistant weeds[EB/OL].Annual Report Internet,2017,http://www.weedscience.com.
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[12]董舒超,胡慧贞,彭良才,等.植物百草枯抗性机理研究进展[J].植物生理学报,2015(9):1373-1380.
[13]凌进.草铵膦,百草枯,草甘膦对非耕地杂草的防效比较[J].农药,2014(8):613-615.
[14]陈世国,强胜,毛婵娟.草甘膦作用机制和抗性研究进展[J].植物保护,2017,43(2):17-24.
[15]Jeater R S L.Evaluation of paraquat and diquat for weed control in rubber[J].Weed Research,1964,4(2):133-141.
[16]张朝贤,倪汉文,魏守辉,等.杂草抗药性研究进展[J].中国农业科学,2009,42(4):1274-1289.
[17]Bradshaw L D,Padgette S R,Kimball S L,et al.Perspectives on glyphosate resistance[J].Weed Technology,1997,11(1):189-198.
[18]Pratley J,Urwin N,Stanton R,et al.Resistance to glyphosate in Lolium rigidum.L.Bioevaluation[J].Weed Science,1999,47(4):405-411.
[19]Lee L J,Ngim J.A first report of glyphosate-resistant goosegrass(Eleusine indica L.)in Malaysia[J].Pest Management Science,2000,56(4):336-339.
[20]Jalaludin A,Ngim J,Bakai BB,et al.Preliminary findings of potentially resistant goosegrass(Elensine indica L.)to glufosinate-ammonium in Malaysia[J].Weed Biology and Management,2010,10(4):256-260.
[21]苏少泉.草甘膦述评[J].农药,2005,44(4):145-149.
[22]Jalaludin A,Qin Yu,Powles S B.Multiple resistance across glufosinate,glyphosate,paraquat and ACCase-inhibiting herbicides in an Eleusine indica population[J].Weed Research,2015,55(1):82-89.
[23]李珣,陈永,曾勇,等.新型灭生性除草剂草铵膦对柑桔园杂草的防效研究[J].中国南方果树,2010,39(4):28-31.
[24]杨松,刘玉华.20%草铵膦AS防除香蕉园杂草田间药效试验[J].湖北植保,2009(4):40-41.
[25]Cirujeda A,Recasens J,Taberner A.A qualitative quick-test for detection of herbicide resistance to tribenuron-methyl in Papaver rhoeas[J].Weed Research,2001,41(6):523-534.
[26]Duran-Prado M,Osuna M D,Prado R D,et al.Molecular basis of resistance to sulfonylureas in Papaver rhoeas[J].Pesticide Biochemistry&Physiology,2004,79(1):10-17.
[27]杨浩娜,柏连阳.棉田反枝苋和马齿苋对草甘膦的抗药性[J].棉花学报,2014,26(6):492-498.
[28]张猛.田旋花(Convolvolus arvensis L.)对草甘膦耐药性机理研究[D].北京:中国农业科学院,2011.
[29]Mueller T C,Barnett K A,Brosnan J T,et al.Glyphosate-Resistant goosegrass(Eleusine indica L.)Confirmed in Tennessee[J].Weed Science,2011,59(4):562-566.
[30]Kuk Y I,Talbert R E.Cross-and multiple resistance of diclofop-resistant Lolium spp[J].Weed Science,2015,48(Jul 2000):412-419.
[31]Mueller T C,Massey J M,Hayes C L,et al.Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed(Conyza canadensis L.Cronq.)[J].Journal of Agriculture and Food Chemistry,2003,51(3):680-684.
[32]Perez-Jones A,Park K W,Colquhoun J,et al.Identification of glyphosate-resistant italian ryegrass(Lolium multiflorum)in Oregon[J].Weed Science,2005,53(6):775-779.
[33]Ribeiro D N,Gil D,Cruz-Hipolito H E,et al.Rapid assays for detection of glyphosate-resistant Lolium spp[J].Journal of Plant Diseases and Protection,2008,18(21):95-99.
[34]张一宾.近年来全球草铵膦的市场及发展趋向[J].中国农药,2016(4):313-315.
[2]Seng C T,Lun L V,San C T,et al.Initial report of glufosinate and paraquat multiple resistance that evolved in a biotype of goosegrass(Eleusine indica L.)in Malaysia[J].Weed Biology and Management,2010,10(4):229-233.
[3]Alfen N K V,Preston C.Plant Biotic Stress:Weeds[M].Encyclopedia of Agriculture and Food Systems,Oxford:Academic Press,2014:343-348.
[4]An J,Shen X F,Ma Q B,et al.Transcriptome profiling to discover putative genes associated with paraquat resistance in goosegrass(Eleusine indica L.)[J].Plos One,2014,9(6):e99940.
[5]Chuah T S,Tan P K,Ismail B S.Effects of adjuvants and soil microbes on the phytotoxic activity of coumarin in combination with pvanillin on goosegrass(Eleusine indica L.)seedling emergence and growth[J].South African Journal of Botany,2013,84(84):128-133.
[6]Lee L J,Ngim J.A first report of glyphosate-resistant goosegrass(Eleusine indica L.)in Malaysia[J].Pest Management Science,2000,56(4):336-339.
[7]Leach G E,Devine M D,Kirkwood R C,et al.Target enzyme based resistance to acetyl-coenzyme a carboxylase inhibitors in Eleusine indica[J].Pesticide Biochemistry and Physiology,1995,51(2):129-136.
[8]Seng C T,Van L L,San C T,et al.Initial report of glufosinate and paraquat multiple resistance that evolved in a biotype of goosegrass(Eleusine indica L.)in Malaysia[J].Weed Biol Manag,2010,10(4):229-233.
[9]Heap I.International survey of herbicide resistant weeds[EB/OL].Annual Report Internet,2017,http://www.weedscience.com.
[10]Chen J,Huang H,Wei S,et al.Investigating the mechanisms of glyphosate resistance in goosegrass(Elensine indica L.)by RNA-Seq technology[J].Plant Journal,2017,89(2):407.
[11]沈雪峰,胡芳,陈勇,等.牛筋草对百草枯抗性水平初探[J].西南农业学报,2016,29(8):1875-1878.
[12]董舒超,胡慧贞,彭良才,等.植物百草枯抗性机理研究进展[J].植物生理学报,2015(9):1373-1380.
[13]凌进.草铵膦,百草枯,草甘膦对非耕地杂草的防效比较[J].农药,2014(8):613-615.
[14]陈世国,强胜,毛婵娟.草甘膦作用机制和抗性研究进展[J].植物保护,2017,43(2):17-24.
[15]Jeater R S L.Evaluation of paraquat and diquat for weed control in rubber[J].Weed Research,1964,4(2):133-141.
[16]张朝贤,倪汉文,魏守辉,等.杂草抗药性研究进展[J].中国农业科学,2009,42(4):1274-1289.
[17]Bradshaw L D,Padgette S R,Kimball S L,et al.Perspectives on glyphosate resistance[J].Weed Technology,1997,11(1):189-198.
[18]Pratley J,Urwin N,Stanton R,et al.Resistance to glyphosate in Lolium rigidum.L.Bioevaluation[J].Weed Science,1999,47(4):405-411.
[19]Lee L J,Ngim J.A first report of glyphosate-resistant goosegrass(Eleusine indica L.)in Malaysia[J].Pest Management Science,2000,56(4):336-339.
[20]Jalaludin A,Ngim J,Bakai BB,et al.Preliminary findings of potentially resistant goosegrass(Elensine indica L.)to glufosinate-ammonium in Malaysia[J].Weed Biology and Management,2010,10(4):256-260.
[21]苏少泉.草甘膦述评[J].农药,2005,44(4):145-149.
[22]Jalaludin A,Qin Yu,Powles S B.Multiple resistance across glufosinate,glyphosate,paraquat and ACCase-inhibiting herbicides in an Eleusine indica population[J].Weed Research,2015,55(1):82-89.
[23]李珣,陈永,曾勇,等.新型灭生性除草剂草铵膦对柑桔园杂草的防效研究[J].中国南方果树,2010,39(4):28-31.
[24]杨松,刘玉华.20%草铵膦AS防除香蕉园杂草田间药效试验[J].湖北植保,2009(4):40-41.
[25]Cirujeda A,Recasens J,Taberner A.A qualitative quick-test for detection of herbicide resistance to tribenuron-methyl in Papaver rhoeas[J].Weed Research,2001,41(6):523-534.
[26]Duran-Prado M,Osuna M D,Prado R D,et al.Molecular basis of resistance to sulfonylureas in Papaver rhoeas[J].Pesticide Biochemistry&Physiology,2004,79(1):10-17.
[27]杨浩娜,柏连阳.棉田反枝苋和马齿苋对草甘膦的抗药性[J].棉花学报,2014,26(6):492-498.
[28]张猛.田旋花(Convolvolus arvensis L.)对草甘膦耐药性机理研究[D].北京:中国农业科学院,2011.
[29]Mueller T C,Barnett K A,Brosnan J T,et al.Glyphosate-Resistant goosegrass(Eleusine indica L.)Confirmed in Tennessee[J].Weed Science,2011,59(4):562-566.
[30]Kuk Y I,Talbert R E.Cross-and multiple resistance of diclofop-resistant Lolium spp[J].Weed Science,2015,48(Jul 2000):412-419.
[31]Mueller T C,Massey J M,Hayes C L,et al.Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed(Conyza canadensis L.Cronq.)[J].Journal of Agriculture and Food Chemistry,2003,51(3):680-684.
[32]Perez-Jones A,Park K W,Colquhoun J,et al.Identification of glyphosate-resistant italian ryegrass(Lolium multiflorum)in Oregon[J].Weed Science,2005,53(6):775-779.
[33]Ribeiro D N,Gil D,Cruz-Hipolito H E,et al.Rapid assays for detection of glyphosate-resistant Lolium spp[J].Journal of Plant Diseases and Protection,2008,18(21):95-99.
[34]张一宾.近年来全球草铵膦的市场及发展趋向[J].中国农药,2016(4):313-315.