光谱法研究一种葡萄糖酰胺阳离子表面活性剂与BSA的相互作用
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摘要
采用荧光猝灭和三维荧光光谱法研究了N,N-二甲基-N[3-(葡萄糖酰胺基)]丙基-N-烷基溴化铵(C_(12)DGPB)与牛血清白蛋白(BSA)的相互作用。结果表明:当NaCl的浓度为0.02 mol·L~(-1),作用时间为15 min和pH值为6.7时,为C_(12)DGPB与BSA相互作用的较佳条件;通过计算得到了不同温度下C_(12)DGPB对BSA的荧光猝灭动态常数、结合参数和热力学参数;由热力学参数确定它们之间的作用力主要是氢键和范德华力,三维荧光光谱显示C_(12)DGPB对酪氨酸和色氨酸的疏水微环境影响较大。
The interaction between the N,N-dimethyl-N-[3-(gluconamide)]propyl-N-alkylammonium bromides(C_(12)DGPB)and bovine serum albumin(BSA)was investigated by fluorescence quenching and three-dimensional fluorescence spectroscopy.The results showed that the optimal condition for interaction between C_(12)DGPB and BSA was NaCl(0.02 mol·L~(-1)),the action time 15 min and pH=6.7.The dynamic quenching constants,binding parameters and thermodynamic parameters of C_(12)DGPB with BSA at different temperatures were calculated.Hydrogen Bond and Van der Waals Force were main forces in the interaction between C_(12)DGPB and BSA through thermodynamic parameters.Three-dimensional fluorescence spectra show that C_(12)DGPB has a great influence on the hydrophobic microenvironment of tyrosine and tryptophan.
The interaction between the N,N-dimethyl-N-[3-(gluconamide)]propyl-N-alkylammonium bromides(C_(12)DGPB)and bovine serum albumin(BSA)was investigated by fluorescence quenching and three-dimensional fluorescence spectroscopy.The results showed that the optimal condition for interaction between C_(12)DGPB and BSA was NaCl(0.02 mol·L~(-1)),the action time 15 min and pH=6.7.The dynamic quenching constants,binding parameters and thermodynamic parameters of C_(12)DGPB with BSA at different temperatures were calculated.Hydrogen Bond and Van der Waals Force were main forces in the interaction between C_(12)DGPB and BSA through thermodynamic parameters.Three-dimensional fluorescence spectra show that C_(12)DGPB has a great influence on the hydrophobic microenvironment of tyrosine and tryptophan.
引文
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[2]Zhu B W,Nicolas D,Chen G R,et al.Direct aldolization of unprotected fructose to bio-based surfactants[J].ACS Sustainable Chem Eng,2018,6,11695-11703.
[3]任龙芳,惠林涛,张融,等.葡萄糖型阴离子表面活性剂的合成、表征及起泡性能[J].精细化工,2015,32(9):985-989.
[4]Lu B,Miao Y,Vigneron P,et al.Measurement of cytotoxicity and irritancy potential of sugar-based surfactants on skin–related 3D models[J].Toxic in Vitro,2017,40(2):305-312.
[5]Zhi L F,Li Q X,Li Y L,et al.Self-aggregation and antimicrobial activity of saccharide-cationic surfactants[J].Colloids and Surfaces A:Phys and Eng Aspects,2014,456(20):231-237.
[6]Zhi L F,Li Q X,Li Y L,et al.Adsorption and aggregation properties of novel star-shaped gluconamide type cationic surfactants in aqueous solution[J].Coll Poly Sci,2014,292(5):1041-1050.
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[8]Zhi L F,Li Q X,Sun Y Q,et al.Mixed stability and antimicrobial properties of gluconamide-type cationic surfactants[J].J Surf Dete,2016.19(2):337-342.
[9]周能,周振,程海燕.大黄素铜锌配合物与牛血清白蛋白的相互作用研究[J].化学研究与应用,2017,29(9):1289-1294.
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[12]马雪红,宋百灵,肖文浚,等.蒜氨酸及人血清白蛋白的相互作用[J].光谱学与光谱分析,2017,37(6):1826-1830.
[13]王永刚,杨光瑞,马雪青,等.荧光光谱法和分子模拟技术研究考马斯亮蓝G-250与牛血清白蛋白的相互作用[J].光谱学与光谱分析,2017,37(8):2427-2479.
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[28]方庆,董澄宇,王宇,等.光谱法和分子对接方法研究罗利环素与人血清白蛋白的相互作用[J].光谱学与光谱分析,2018,38(3):990-996.
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[31]Li T,Cheng Z J,Cao L J,et al.Comparison of interactions between three food colorants and BSA[J].Food Chem,2016,194(3):740-748.