欧李优系组培快繁技术研究
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摘要
为建立欧李(Cerasus humilis)优系离体快繁技术体系,对其离体培养过程中初代培养、增殖继代培养、生根培养3个阶段的培养条件进行了研究。结果表明,以其半木质化茎段为外植体,最适初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L IBA,最适增殖培养基为MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.05 mg/L IBA+300 mg/L水解乳蛋白,最适生根培养基为1/2MS+0.2 mg/L IBA+0.5 mg/L IAA。
In order to establish the technical system of in vitro and rapid propagation of Cerasus humilis,the culture conditions of the first generation culture, the proliferation culture and the rooting culture were studied. The semi-lignified stem segments were used as explants. The results showed that the optimum medium of the first generation medium was MS+0.5 mg/L6-BA+0.1 mg/L IBA,the optimum proliferation medium was MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.05 mg/L IBA+300 mg/L LH,and the optimum proliferation medium was 1/2 MS+0.2 mg/L IBA+0.5 mg/L IAA.
In order to establish the technical system of in vitro and rapid propagation of Cerasus humilis,the culture conditions of the first generation culture, the proliferation culture and the rooting culture were studied. The semi-lignified stem segments were used as explants. The results showed that the optimum medium of the first generation medium was MS+0.5 mg/L6-BA+0.1 mg/L IBA,the optimum proliferation medium was MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.05 mg/L IBA+300 mg/L LH,and the optimum proliferation medium was 1/2 MS+0.2 mg/L IBA+0.5 mg/L IAA.
引文
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[2]张东为,贾天会,舒乔生.水保优良树种欧李的研究进展及今后研究方向[J].中国水土保持,2012,34(1):45-47.
[3]刘明,穆晓鹏,薛晓芳,等.欧李生物技术研究进展及其应用展望[J].落叶果树,2010,42(5):15-17.
[4]庄丽娟,苏福才.欧李的组织培养与快速繁殖技术[J].内蒙古农业大学学报,2005,26(1):16-19.
[5]张秀丽.欧李组织培养体系试验研究[D].河北保定:河北农业大学,2007.
[6]孙新政,申顺先,李庆伟,等.钙果4号欧李组织培养技术研究[J].果树学报,2007,24(1):80-83.
[7]曹琴,杜俊杰.欧李组培苗移栽成活影响因子的研究[J].山西农业大学学报,2009,29(3):238-242.
[8]王有信,何卫军,李向东,等.欧李种质资源分布及种群分类特性研究[J].山西果树,2005,25(6):36-38.
[9]周金梅.欧李芽离体诱导培养技术研究[J].江苏农业科学,2011,39(6):97,110.
[10]焦淑华,林丽华,李宝江,等.欧李茎尖组织培养研究[J].沈阳农业大学学报,2006,37(4):573-577.
[11]黄立华,龙忠伟,张平.欧李组织培养技术的研究[J].中国新技术新产品,2009(11):227.
[12]梁伟玲,卢彦琦,陈翠果,等.欧李优系试管苗增殖关键技术研究[J].北方园艺,2016(17):103-106.
[13]范小峰,田兴旺,王根旺.欧李快繁与生产中商品苗产率问题研究[J].中国水土保持,2006(8):49-50.