阳起石、菟丝子与淫羊藿对肾阳虚大鼠性激素及其受体表达的影响
|
摘要
为了比较阳起石、菟丝子、淫羊藿对肾阳虚模型大鼠的治疗效果,通过肌肉注射氢化可地松制作了肾阳虚大鼠模型,并在3种药物治疗前后分别对体质量、脏器指数、子宫角长度、子宫中段外径、激素水平及其受体基因的表达情况进行了比较研究。结果显示,阳起石、菟丝子、淫羊藿均能够显著增加大鼠的肝脏指数,提高大鼠黄体生成素、卵泡刺激素、雌激素及激素受体的表达水平,其中阳起石与菟丝子对激素分泌的调节优于淫羊藿,而阳起石与淫羊藿对激素受体表达的调节优于菟丝子。结果表明,这3种药物能通过促进激素分泌和激素受体的表达来改善肾阳虚动物子宫和卵巢的功能,且3种药物的疗效各有优势。
To compare the treatment efficacy of Actinolite,Cuscutae Semen and Epimedii Folium,the rat model of kidney-yang deficiency was founded through intramuscular injection of hydrocortisone.Before and after treatment with three drugs,body weight,organ index,uterine angle length,diameter of uterus,hormone level and gene expression of hormone receptor were detected respectively.Actinolite and Cuscutae Semen regulation of hormone secretion was better than Epimedii Folium,and Actinolite and Epimedium Folium hormone receptor expression was more obvious than Cuscutae Semen.The results showed that Actinolite,Cuscutae Semen and Epimedii Folium,increased liver index,and the levels of luteinizing hormone,follicle-stimulating hormone,estrogen,and gene expression levels of hormone receptor.These results indicated three drugs improved functions of ovary and uterus through promoting hormone secretion and gene expression of hormone receptors,and the efficacy of the three drugs has a specific advantage,respectively.
To compare the treatment efficacy of Actinolite,Cuscutae Semen and Epimedii Folium,the rat model of kidney-yang deficiency was founded through intramuscular injection of hydrocortisone.Before and after treatment with three drugs,body weight,organ index,uterine angle length,diameter of uterus,hormone level and gene expression of hormone receptor were detected respectively.Actinolite and Cuscutae Semen regulation of hormone secretion was better than Epimedii Folium,and Actinolite and Epimedium Folium hormone receptor expression was more obvious than Cuscutae Semen.The results showed that Actinolite,Cuscutae Semen and Epimedii Folium,increased liver index,and the levels of luteinizing hormone,follicle-stimulating hormone,estrogen,and gene expression levels of hormone receptor.These results indicated three drugs improved functions of ovary and uterus through promoting hormone secretion and gene expression of hormone receptors,and the efficacy of the three drugs has a specific advantage,respectively.
引文
[1] WALSH S W,WILLIAMS E J,EVANS A C O.A review of the causes of poor fertility in high milk producing dairy cows[J].Anim Reprod Sci,2011,123(3/4):127-138.
[2] DOUNGRUT T,FUNGBUN N,LAOHARATCHATATHANIN T,et al.Effects of gonadotropin-releasing hormone agonist on human chorionic gonadotropin activity in granulosa cells of immature female rats[J].J Reprod Dev,2018,64(2):129-134.
[3] 庞书琴.奶牛不孕症的病因分析及治疗措施[J].华北农学报,2005(S1):209-211.
[4] 刘博,沈媛,毛伟,等.前列腺素E2和F2α对LPS刺激后奶牛子宫内膜上皮细胞COX-1和COX-2表达的影响[J].中国畜牧兽医,2017(9):2716-2723.
[5] 龙泳伶.拆方肾气丸对肾阳虚雌鼠靶腺轴调控的实验及临床研究[D].广州:广州中医药大学,2013.
[6] 王东升,严作廷,李世宏,等.治疗奶牛卵巢疾病性不孕症中药制剂研究进展[J].中国奶牛,2011(22):35-38.
[7] 中国农业科学院中兽医研究所.兽医中药学[M].北京:农业出版社,1965.
[8] DONNAPEE S,LI J,YANG X,et al.Cuscuta chinensis Lam.:A systematic review on ethnopharmacology,phytochemistry and pharmacology of an important traditional herbal medicine[J].J Ethnopharmacol,2014,157:292-308.
[9] 房凌云.淫羊藿的药理作用及临床应用进展[J].智慧健康,2017,3(21):25-27.
[10] 王凯,刘帅帅,郭起岳,等.氢化可的松致SD大鼠肾阳虚证卵巢早衰模型研究[J].成都医学院学报,2016(1):10-15.
[11] 徐红艳,鞠玉琳,张敏,等.壮阳促孕散对雌性肾阳虚大鼠垂体—性腺轴分泌激素的影响[J].湖北农业科学,2011(20):4240-4241.
[12] 黄厚才.金匮肾气丸对大鼠肾阳虚模型的影响[J].中国比较医学杂志,2004.
[13] 肖静,何立群,高建东,等.腺嘌呤与氢化可的松大鼠肾阳虚模型造模方法比较[J].中国比较医学杂志,2008(3):77-80.
[14] 张世栋,王旭荣,杨峰,等.催情助孕液对小鼠雌、孕激素水平及其受体mRNA表达的影响[J].中国兽医学报,2013,33(7):1127-1131.
[15] HAN H,SUN Z,LUO G,et al.Fluoride exposure changed the structure and the expressions of reproductive related genes in the hypothalamus-pituitary-testicular axis of male mice[J].Chemosphere,2015,135:297-303.
[16] 亓琦,王瑞雯,梁红娟,等.补肾壮元胶囊对肾阳虚小鼠性激素水平及免疫功能影响的实验研究[J].中国中医药科技,2015(05):493-495.
[17] 邹海淼,辛雪,孙伟,等.肾阳虚生化指标的现代研究进展[J].现代生物医学进展,2015(30):5989-5993.
[18] 沙爱龙,吴瑛,盛海燕,等.昆仑雪菊黄酮对衰老模型小鼠脑及脏器指数的影响[J].动物医学进展,2013(7):66-68.
[19] 杨鉴冰,崔晓萍,王宗柱,等.毓宫胶囊对肾阳虚大鼠子宫雌激素受体的影响[J].中医药学刊,2003(8):1280-1282.
[20] 谭萍.大鼠颗粒细胞InhibinA抑制FSHR表达抑制FSH的作用[D].重庆:西南大学,2008.
[21] 徐庚全,樊江峰,杨世华,等.FSH、LH对体外培养的牦牛卵泡颗粒细胞凋亡及E_2、P分泌功能的影响[J].畜牧兽医学报,2015(6):932-939.
[22] WEI S,GONG Z,GUO H,et al.FSH and eCG impact follicles development and expression of ovarian FSHR and caspase-9 in mice[J].Iran J Vet Res,2017,18(2):79-85.
[23] 赖爱鸾,孙艳格,张李松,等.性激素、年龄、月经模式变化对卵巢储备功能的预测价值[J].中国实用妇科与产科杂志,2010(9):717-719.
[24] WANG Y,FENG H,BI C,et al.GSK-3beta mediates in the progesterone inhibition of estrogen induced cyclin D2 nuclear localization and cell proliferation in cyclin D1-/- mouse uterine epithelium[J].FEBS Lett,2007,581(16):3069-3075.
[25] 白宇,张井,胡景炎,等.牛奶中雌二醇胶体金试纸条快速检测技术研究[J].中国畜牧兽医,2017(11):3351-3357.
[26] Huang W W,Yin Y,Bi Q,et al.Developmental diethylstilbestrol exposure alters genetic pathways of uterine cytodifferentiation[J].Mol Endocrinol,2005,19(3):669-682.
[27] 王文芳,何玉琴,韩志磊,等.高原甘加型藏羊发情周期不同阶段卵巢FSHR和LHR基因表达差异的研究[J].中国兽医科学,2016(1):97-103.
[2] DOUNGRUT T,FUNGBUN N,LAOHARATCHATATHANIN T,et al.Effects of gonadotropin-releasing hormone agonist on human chorionic gonadotropin activity in granulosa cells of immature female rats[J].J Reprod Dev,2018,64(2):129-134.
[3] 庞书琴.奶牛不孕症的病因分析及治疗措施[J].华北农学报,2005(S1):209-211.
[4] 刘博,沈媛,毛伟,等.前列腺素E2和F2α对LPS刺激后奶牛子宫内膜上皮细胞COX-1和COX-2表达的影响[J].中国畜牧兽医,2017(9):2716-2723.
[5] 龙泳伶.拆方肾气丸对肾阳虚雌鼠靶腺轴调控的实验及临床研究[D].广州:广州中医药大学,2013.
[6] 王东升,严作廷,李世宏,等.治疗奶牛卵巢疾病性不孕症中药制剂研究进展[J].中国奶牛,2011(22):35-38.
[7] 中国农业科学院中兽医研究所.兽医中药学[M].北京:农业出版社,1965.
[8] DONNAPEE S,LI J,YANG X,et al.Cuscuta chinensis Lam.:A systematic review on ethnopharmacology,phytochemistry and pharmacology of an important traditional herbal medicine[J].J Ethnopharmacol,2014,157:292-308.
[9] 房凌云.淫羊藿的药理作用及临床应用进展[J].智慧健康,2017,3(21):25-27.
[10] 王凯,刘帅帅,郭起岳,等.氢化可的松致SD大鼠肾阳虚证卵巢早衰模型研究[J].成都医学院学报,2016(1):10-15.
[11] 徐红艳,鞠玉琳,张敏,等.壮阳促孕散对雌性肾阳虚大鼠垂体—性腺轴分泌激素的影响[J].湖北农业科学,2011(20):4240-4241.
[12] 黄厚才.金匮肾气丸对大鼠肾阳虚模型的影响[J].中国比较医学杂志,2004.
[13] 肖静,何立群,高建东,等.腺嘌呤与氢化可的松大鼠肾阳虚模型造模方法比较[J].中国比较医学杂志,2008(3):77-80.
[14] 张世栋,王旭荣,杨峰,等.催情助孕液对小鼠雌、孕激素水平及其受体mRNA表达的影响[J].中国兽医学报,2013,33(7):1127-1131.
[15] HAN H,SUN Z,LUO G,et al.Fluoride exposure changed the structure and the expressions of reproductive related genes in the hypothalamus-pituitary-testicular axis of male mice[J].Chemosphere,2015,135:297-303.
[16] 亓琦,王瑞雯,梁红娟,等.补肾壮元胶囊对肾阳虚小鼠性激素水平及免疫功能影响的实验研究[J].中国中医药科技,2015(05):493-495.
[17] 邹海淼,辛雪,孙伟,等.肾阳虚生化指标的现代研究进展[J].现代生物医学进展,2015(30):5989-5993.
[18] 沙爱龙,吴瑛,盛海燕,等.昆仑雪菊黄酮对衰老模型小鼠脑及脏器指数的影响[J].动物医学进展,2013(7):66-68.
[19] 杨鉴冰,崔晓萍,王宗柱,等.毓宫胶囊对肾阳虚大鼠子宫雌激素受体的影响[J].中医药学刊,2003(8):1280-1282.
[20] 谭萍.大鼠颗粒细胞InhibinA抑制FSHR表达抑制FSH的作用[D].重庆:西南大学,2008.
[21] 徐庚全,樊江峰,杨世华,等.FSH、LH对体外培养的牦牛卵泡颗粒细胞凋亡及E_2、P分泌功能的影响[J].畜牧兽医学报,2015(6):932-939.
[22] WEI S,GONG Z,GUO H,et al.FSH and eCG impact follicles development and expression of ovarian FSHR and caspase-9 in mice[J].Iran J Vet Res,2017,18(2):79-85.
[23] 赖爱鸾,孙艳格,张李松,等.性激素、年龄、月经模式变化对卵巢储备功能的预测价值[J].中国实用妇科与产科杂志,2010(9):717-719.
[24] WANG Y,FENG H,BI C,et al.GSK-3beta mediates in the progesterone inhibition of estrogen induced cyclin D2 nuclear localization and cell proliferation in cyclin D1-/- mouse uterine epithelium[J].FEBS Lett,2007,581(16):3069-3075.
[25] 白宇,张井,胡景炎,等.牛奶中雌二醇胶体金试纸条快速检测技术研究[J].中国畜牧兽医,2017(11):3351-3357.
[26] Huang W W,Yin Y,Bi Q,et al.Developmental diethylstilbestrol exposure alters genetic pathways of uterine cytodifferentiation[J].Mol Endocrinol,2005,19(3):669-682.
[27] 王文芳,何玉琴,韩志磊,等.高原甘加型藏羊发情周期不同阶段卵巢FSHR和LHR基因表达差异的研究[J].中国兽医科学,2016(1):97-103.