金花茶种子对α-葡萄糖苷酶活性的抑制作用研究
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摘要
目的 研究金花茶种子乙醇提取物及醇提物的乙酸乙酯部分、正丁醇部分及水溶部分三个极性部位对α-葡萄糖苷酶的抑制作用。方法 采用体外抑制α-葡萄糖苷酶活性模型测定活性并进行酶抑制动力学研究,通过Lineweave-Burk作图法确定抑制类型。结果 金花茶种子乙醇提取物、乙酸乙酯部分、正丁醇部分及水溶部分均有抑制α-葡萄糖苷酶作用,其IC_(50)分别为37.74、335.90、20.53、17.80μg/ml,活性均高于阳性对照药阿卡波糖(484.90μg/ml)。其中阿卡波糖为竞争性抑制剂,其余均为非竞争性抑制剂。结论 金花茶种子为首次报道对α-葡萄糖苷酶有抑制活性,而正丁醇部分与水层部分为其抑制α-葡萄糖苷酶的有效部位。
Objective The inhibition of α-glucosidase by the three polar moieties of the ethyl acetate, n-butanol and water soluble fractions of ethanol extracts and alcohol extracts of Camellia nitidissima Chi. seeds was studied. Methods The in vitro inhibition of α-glucosidase activity model was used to determine the activity and enzyme inhibition kinetics were studied, and the type of inhibition was determined by Lineweave-Burk plotting. Results The ethanol extract, ethyl acetate fraction, n-butanol fraction, and water soluble fraction of Camellia nitidissima Chi. all inhibited α-glucosidase activity. The IC_(50) values were 37.74, 335.90, 20.53, and 17.8 μg/ml, respectively. Activity is higher than the positive control drug Acarbose(484.9 μg/ml). Acarbose is a competitive inhibitor, and the rest are non-competitive inhibitors. Conclusion The seeds of Camellia nitidissima Chi. were reported for the first time to inhibit the α-glucosidase activity, while the n-butanol part and the aqueous layer part were effective sites for inhibiting α-glucosidase.
Objective The inhibition of α-glucosidase by the three polar moieties of the ethyl acetate, n-butanol and water soluble fractions of ethanol extracts and alcohol extracts of Camellia nitidissima Chi. seeds was studied. Methods The in vitro inhibition of α-glucosidase activity model was used to determine the activity and enzyme inhibition kinetics were studied, and the type of inhibition was determined by Lineweave-Burk plotting. Results The ethanol extract, ethyl acetate fraction, n-butanol fraction, and water soluble fraction of Camellia nitidissima Chi. all inhibited α-glucosidase activity. The IC_(50) values were 37.74, 335.90, 20.53, and 17.8 μg/ml, respectively. Activity is higher than the positive control drug Acarbose(484.9 μg/ml). Acarbose is a competitive inhibitor, and the rest are non-competitive inhibitors. Conclusion The seeds of Camellia nitidissima Chi. were reported for the first time to inhibit the α-glucosidase activity, while the n-butanol part and the aqueous layer part were effective sites for inhibiting α-glucosidase.
引文
[1] 广西科学院广西植物研究所.广西植物志[M].南宁:广西科技出版社,1965:780.
[2] Chen YY,Huang YL,Wen YX.Advance in study on Chemical Constituents and Pharmacological Action of Camellia chrysantha[J].Guangxi Tropical Agriculture ,2009,1:14.
[3] Yang R,Guan Y,Wang WX,et al.Antioxidant capacity of phenolics in Camellia nitidissima Chi flowers and their identification by HPLC Triple TOF MS/MS [J] .PLoS ONE,2018,13(4):e0195508.
[4] Morrison F,Shubina M,Turchin A.Encounter frequency and serum glu-cose level,blood pressure,and cholesterol level control in patients with diabetes mellitus[J].Arch of Int Med,2001,171,1542.
[5] 马永雷,张雨青,周丽霞,等.桑枝皮醇提物的抗氧化和对α-糖苷酶活性的抑制作用[J].蚕业科学,2010,36(1):143.
[7] 顾江萍,梁鑫淼.中药对α-葡萄糖苷酶抑制作用的研究[J].中华现代中医学杂志,2005,1(2):116.
[8] Li T,Zhang XD,Song YW,et al.A microplate-based screening methA for alpha-glucosidase inhibitors [J].Chin J Clin Pharmacol Ther.2005,10(10):1129.
[9] Deng YT,Lin-Shiau SY,Shyur LF,et al.Pu-erh tea polysaccharides decrease blood sugar by inhibition of α-glucosidase activity in vitro and in mice [J].Food Funct,`2015,6(5):1539.
[10] 于彩云,高兆兰,陈天姿,等.天然产物中α-葡萄糖苷酶抑制剂的研究进展[J].食品工业科技,2015,36(22):394.
[2] Chen YY,Huang YL,Wen YX.Advance in study on Chemical Constituents and Pharmacological Action of Camellia chrysantha[J].Guangxi Tropical Agriculture ,2009,1:14.
[3] Yang R,Guan Y,Wang WX,et al.Antioxidant capacity of phenolics in Camellia nitidissima Chi flowers and their identification by HPLC Triple TOF MS/MS [J] .PLoS ONE,2018,13(4):e0195508.
[4] Morrison F,Shubina M,Turchin A.Encounter frequency and serum glu-cose level,blood pressure,and cholesterol level control in patients with diabetes mellitus[J].Arch of Int Med,2001,171,1542.
[5] 马永雷,张雨青,周丽霞,等.桑枝皮醇提物的抗氧化和对α-糖苷酶活性的抑制作用[J].蚕业科学,2010,36(1):143.
[7] 顾江萍,梁鑫淼.中药对α-葡萄糖苷酶抑制作用的研究[J].中华现代中医学杂志,2005,1(2):116.
[8] Li T,Zhang XD,Song YW,et al.A microplate-based screening methA for alpha-glucosidase inhibitors [J].Chin J Clin Pharmacol Ther.2005,10(10):1129.
[9] Deng YT,Lin-Shiau SY,Shyur LF,et al.Pu-erh tea polysaccharides decrease blood sugar by inhibition of α-glucosidase activity in vitro and in mice [J].Food Funct,`2015,6(5):1539.
[10] 于彩云,高兆兰,陈天姿,等.天然产物中α-葡萄糖苷酶抑制剂的研究进展[J].食品工业科技,2015,36(22):394.