摘要
目的:探讨血热型银屑病患者外周血单个核细胞PGC-1α的表达,研究PGC-1α的表达对银屑病发病的影响,从而揭示线粒体障碍对银屑病的调节机制。方法:设立中药治疗组与健康对照组,收集寻常型血热型银屑病患者,治疗组予凉血消风汤颗粒6 g,2次/d连续服用4周后,用Western Blot检测PGC-1α蛋白表达量,用流式细胞术检测细胞凋亡率。结果:银屑病治疗前PGC-1α较正常组下调(P<0.05,差异有统计学意义),细胞凋亡较正常增多(P<0.05,差异有统计学意义),治疗后PGC-1α表达上调(P<0.05,差异有统计学意义),细胞凋亡较前降低(P<0.05,差异有统计学意义)。结论:凉血消风汤能增加体内PGC-1α含量,降低细胞凋亡率,其机制可能与减少线粒体障碍相关。
Objective:To investigate the expression of PGC-1α in peripheral blood mononuclear cells in patients with blood-heat type psoriasis and investigate the effect of PGC-1α expression on the pathogenesis of psoriasis so as to reveal the mechanism of mitochondrial disturbance on psoriasis.Methods:A Chinese medicine treatment group and a healthy control group were set up.The patients with psoriasis vulgaris were collected.The treatment group was treated with Liangxue Xiaofeng Decoction 6 g twice a day for 4 weeks.The expression of PGC-1α protein was detected by Western Blot.The apoptosis rate was measured by flow cytometry.Results:PGC-1α in the psoriatic group was significantly lower than that before treatment(P<0.05).PGC-1α expression after treatment was up-regulated(P<0.05) and the difference was statistically significant.The apoptosis was lower than that before treatment(P<0.05).Conclusion:Liangxue Xifeng Decoction can increase the content of PGC-1α and reduce the rate of cell apoptosis.The mechanism may be related to the decrease of mitochondrial dysfunction.
引文
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