PGC-1α在寻常型血热型银屑病中的调节机制
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摘要
目的:探讨血热型银屑病患者外周血单个核细胞PGC-1α的表达,研究PGC-1α的表达对银屑病发病的影响,从而揭示线粒体障碍对银屑病的调节机制。方法:设立中药治疗组与健康对照组,收集寻常型血热型银屑病患者,治疗组予凉血消风汤颗粒6 g,2次/d连续服用4周后,用Western Blot检测PGC-1α蛋白表达量,用流式细胞术检测细胞凋亡率。结果:银屑病治疗前PGC-1α较正常组下调(P<0.05,差异有统计学意义),细胞凋亡较正常增多(P<0.05,差异有统计学意义),治疗后PGC-1α表达上调(P<0.05,差异有统计学意义),细胞凋亡较前降低(P<0.05,差异有统计学意义)。结论:凉血消风汤能增加体内PGC-1α含量,降低细胞凋亡率,其机制可能与减少线粒体障碍相关。
Objective:To investigate the expression of PGC-1α in peripheral blood mononuclear cells in patients with blood-heat type psoriasis and investigate the effect of PGC-1α expression on the pathogenesis of psoriasis so as to reveal the mechanism of mitochondrial disturbance on psoriasis.Methods:A Chinese medicine treatment group and a healthy control group were set up.The patients with psoriasis vulgaris were collected.The treatment group was treated with Liangxue Xiaofeng Decoction 6 g twice a day for 4 weeks.The expression of PGC-1α protein was detected by Western Blot.The apoptosis rate was measured by flow cytometry.Results:PGC-1α in the psoriatic group was significantly lower than that before treatment(P<0.05).PGC-1α expression after treatment was up-regulated(P<0.05) and the difference was statistically significant.The apoptosis was lower than that before treatment(P<0.05).Conclusion:Liangxue Xifeng Decoction can increase the content of PGC-1α and reduce the rate of cell apoptosis.The mechanism may be related to the decrease of mitochondrial dysfunction.
Objective:To investigate the expression of PGC-1α in peripheral blood mononuclear cells in patients with blood-heat type psoriasis and investigate the effect of PGC-1α expression on the pathogenesis of psoriasis so as to reveal the mechanism of mitochondrial disturbance on psoriasis.Methods:A Chinese medicine treatment group and a healthy control group were set up.The patients with psoriasis vulgaris were collected.The treatment group was treated with Liangxue Xiaofeng Decoction 6 g twice a day for 4 weeks.The expression of PGC-1α protein was detected by Western Blot.The apoptosis rate was measured by flow cytometry.Results:PGC-1α in the psoriatic group was significantly lower than that before treatment(P<0.05).PGC-1α expression after treatment was up-regulated(P<0.05) and the difference was statistically significant.The apoptosis was lower than that before treatment(P<0.05).Conclusion:Liangxue Xifeng Decoction can increase the content of PGC-1α and reduce the rate of cell apoptosis.The mechanism may be related to the decrease of mitochondrial dysfunction.
引文
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[12] Sunitha S,Rajappa M,Thappa D M,et al.Is the ratio of antibodies against oxidized LDL to oxidized LDL an indicator of cardiovascular risk in psoriasis?[J].Oman medical journal,2016,31(5):390.
[13] Schieber M,Chandel N S.ROS function in redox signaling and oxidative stress[J].Current biology,2014,24(10):R453-R462.
[14] 丁素先,许振毅.边天羽论文集[M].天津:天津科学技术出版社,1999:6.
[2] Sharma S,Anjaneyulu M,Kulkarni SK,et al.Resveratrol,a polyphenolic phytoalexin,attenuates diabetic nephropathy in rats[J].Pharmacology,2006,76(2):69-75.
[3] Togliatto G,Lombardo G,Brizzi M F.The future challenge of reactive oxygen species (ROS) in hypertension:from bench to bed side[J].International journal of molecular sciences,2017,18(9):1988.
[4] Adibhatla R M,Hatcher J F.Phospholipase A2,reactive oxygen species,and lipid peroxidation in cerebral ischemia[J].Free Radical Biology and Medicine,2006,40(3):376-387.
[5] Zhuo L1,Fu B,Bai X,et al.NAD blocks high glucose induced mesangial hypertrophy via activation of the sirtuins-AMPK-m TOR pathway[J].Cell Physiol Biochem,2011,27(6):681-690.
[6] Jiang X,Jiang H,Shen Z,et al.Activation of mitochondrial protease OMA1 by Bax and Bak promotes cytochrome c release during apoptosis[J].Proceedings of the National Academy of Sciences,2014,111(41):14782-14787.
[7] Wang Y,Zhao X,Lotz M,et al.Mitochondrial biogenesis is impaired in osteoarthritis chondrocytes but reversible via peroxisome proliferator-activated receptor gamma coactivator 1alpha[J].Arthritis Rheumatol,2015,67(8):2141-2153.
[8] Kang C,Li Ji L.Role of PGC-1alpha signaling in skeletal muscle health and disease[J].Annals of the New York Academy of Sciences,2012,1271:110-117.
[9] Choi EM,Suh KS,Rhee SY,et al.Sciadopitysin alleviates methylglyoxal mediated glycation in osteoblastic MC3T3-E1 cells by enhancingglyoxalase system and mitochondrial biogenesis[J].Free Radic Res,2014,48(7):729-739.
[10] Xu Y,Nie L,Yin YG,et al.Resveratrol protects against hyperglycemia induced oxidative damage to mitochondria by activating SIRT1 in rat mesangial cells[J].Toxicol Appl Pharmacol,2012,259(3):395-401.
[11] Cho J W,Lee K S,Kim C W.Curcumin attenuates the expression of IL-1β,IL-6,and TNF-α as well as cyclin E in TNF-α-treated HaCaT cells;NF-κB and MAPKs as potential upstream targets[J].International journal of molecular medicine,2007,19(3):469-474.
[12] Sunitha S,Rajappa M,Thappa D M,et al.Is the ratio of antibodies against oxidized LDL to oxidized LDL an indicator of cardiovascular risk in psoriasis?[J].Oman medical journal,2016,31(5):390.
[13] Schieber M,Chandel N S.ROS function in redox signaling and oxidative stress[J].Current biology,2014,24(10):R453-R462.
[14] 丁素先,许振毅.边天羽论文集[M].天津:天津科学技术出版社,1999:6.