PTEN、PAX-2、EMP2在子宫内膜增生症中的表达及意义
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摘要
目的探讨人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)、配对盒基因(PAX)-2、上皮细胞膜蛋白2(EMP2)在子宫内膜增生症中的表达情况,探讨其与子宫内膜增生症的关系及临床意义。方法应用免疫组化法检测子宫内膜增生不伴非典型增生(即良性增生)子宫内膜组织、子宫内膜非典型增生/子宫内膜上皮内瘤变(EIN)子宫内膜组织、正常增生期子宫内膜组织中PTEN、PAX-2、EMP2蛋白表达情况。结果在子宫内膜增生症中,PTEN和PAX-2表达明显减弱或缺失表达,与正常增生期子宫内膜比较差异具有统计学意义(P均<0.05)。EMP2在子宫内膜增生症中表达明显增强,与正常增生期子宫内膜比较差异具有统计学意义(P<0.05)。结论 PTEN、PAX-2、EMP2的异常表达与子宫内膜增生症的发生相关,对子宫内膜增生症的诊断具有一定辅助意义。
Objective It is to investigate the expression of PTEN, PAX-2 and EMP2 in endometrial hyperplasia, and to explore its relationship with endometrial hyperplasia and its clinical significance. Methods Immunohistochemistry was used to detect the expression of PTEN, PAX-2 and EMP2 proteins in endometrial tissue of endometrial hyperplasia without atypical hyperplasia(benign hyperplasia), endometrial atypical hyperplasia/endometrial intraepithelial neoplasia(EIN), and normal proliferative endometrial tissues. Results In the endometrial hyperplasia, the expression of PTEN and PAX-2 was significantly decreased or absent, the difference was statistically significant compared with the normal proliferative endometrium(P<0.05). The expression of EMP2 was significantly enhanced in endometrial hyperplasia, the difference was statistically significant compared with the normal proliferative endometrium(P<0.05). Conclusion The abnormal expression of PTEN, PAX-2 and EMP2 is associated with the occurrence of endometrial hyperplasia, and it has certain auxiliary significance for the diagnosis of endometrial hyperplasia.
Objective It is to investigate the expression of PTEN, PAX-2 and EMP2 in endometrial hyperplasia, and to explore its relationship with endometrial hyperplasia and its clinical significance. Methods Immunohistochemistry was used to detect the expression of PTEN, PAX-2 and EMP2 proteins in endometrial tissue of endometrial hyperplasia without atypical hyperplasia(benign hyperplasia), endometrial atypical hyperplasia/endometrial intraepithelial neoplasia(EIN), and normal proliferative endometrial tissues. Results In the endometrial hyperplasia, the expression of PTEN and PAX-2 was significantly decreased or absent, the difference was statistically significant compared with the normal proliferative endometrium(P<0.05). The expression of EMP2 was significantly enhanced in endometrial hyperplasia, the difference was statistically significant compared with the normal proliferative endometrium(P<0.05). Conclusion The abnormal expression of PTEN, PAX-2 and EMP2 is associated with the occurrence of endometrial hyperplasia, and it has certain auxiliary significance for the diagnosis of endometrial hyperplasia.
引文
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[13] 增林文,孔祥东,卢江昆,等.上皮细胞膜蛋白2在甲状腺乳头状癌中的表达及其对细胞侵袭迁移能力的影响[J].现代肿瘤医学,2018,26(12):1822-1826
[14] 何惠嫦,黄瑾,陈应智,等.EMP2、TTF-1在子宫内膜增生症和子宫内膜样腺癌中的表达及意义[J].海南医学,2017,28(11):1731-1735
[15] Fu M,Rao R,Sudhakar D,et al.Epithelial membrane protein-2 promotes endometrial tumor formation through activation of FAK and Src[J].PLoS One,2011,6(5):el19945
[2] Kurman RJ,Carcangiu ML,Herrington CS,et al.WHO classification of tumours of female reproductive organs[M].4 th ed.Lyon:IARC,2014
[3] 刘颂平,温坚,殷新明.Pax-2在子宫内膜异位症中的表达和意义的研究[J].安徽医学,2012,33(8):953-955
[4] Stewart CJ,Doherty DA,Haolat M,et al.Transtubal sp-read of endometrial carcinoma:correlation of intra-luminal tumor cells with tumour grade,peritoneal fluid cytology,and extra-uterine metastasis[J].Pathology,2013,45(4):382-387
[5] 曹霞,何国照.曼月乐及孕激素治疗子宫内膜增生症的临床疗效比较[J].齐齐哈尔医学院学报,2015,36(12):1737-1738
[6] 李品莹,李小毛.PTEN基因在子宫内膜癌细胞上皮间质转化中的作用[J].实用医学杂志,2018,34(7):1101-1106
[7] 廖山婴,朱小波,王蓓蓓,等.PPARγ、PTEN、Akt在胃癌中的表达及其与胃癌生物学行为的关系[J].新医学,2014,45(6):394-398
[8] Kafshdooz L,Kafshdooz T,Tabrizi AD,et al.Role of exon 7 PTEN Gene in Endometrial Carcinoma[J].Asian Pac J Cancer Prev,2015,16(11):4521-4524
[9] 黄雪云,张丽华,魏晓莹,等.PAX-2、β-catenin及HAND2蛋白在子宫内膜增生症中的表达及其意义[J].东南大学学报:医学版,2017,36(6):919-923
[10] Sanderson PA,Critchley HO,Williams AR,et al.New concepts for an old problem:the diagnosis of endometrial hyperplasis[J].Hum Reprod Update,2017,23(2):232-254
[11] Joiner AK,Quich CM,Jeffus SK.PAX2 expression in simultaneously diagnosed WHO and EIN classification systems[J].Int J Gynecol Pathol,2015,34(1):40-46
[12] Habeeb O,Goodgick L,Soslow RA,et al.Epithelial membrane protein-2 expression is early predictor Ir endometrial cancer development[J].Cancer,2010,116(20):4718-4726
[13] 增林文,孔祥东,卢江昆,等.上皮细胞膜蛋白2在甲状腺乳头状癌中的表达及其对细胞侵袭迁移能力的影响[J].现代肿瘤医学,2018,26(12):1822-1826
[14] 何惠嫦,黄瑾,陈应智,等.EMP2、TTF-1在子宫内膜增生症和子宫内膜样腺癌中的表达及意义[J].海南医学,2017,28(11):1731-1735
[15] Fu M,Rao R,Sudhakar D,et al.Epithelial membrane protein-2 promotes endometrial tumor formation through activation of FAK and Src[J].PLoS One,2011,6(5):el19945