洁净手术室工作人员眼镜带菌状况调查
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摘要
目的了解洁净手术室工作人员佩戴的眼镜带菌状况,以保证洁净手术室空气洁净度,减少手术切口感染发生。方法对经常进入洁净手术室的外科手术医师、麻醉人员和手术室护士三类医务人员佩戴眼镜情况进行问卷调查,并随机对52名人员佩戴的眼镜采样检测,进行菌落计数和细菌鉴定,了解眼镜的带菌状况。并检测应用乙醇擦拭后细菌的清除情况。结果调查全院经常出入洁净手术室的外科手术医师、麻醉人员和手术室护士三类医务人员共180名,总的眼镜佩戴率为56.67%(102/180);共抽查检测52副眼镜,其中携带细菌菌落数最高为108CFU/cm2,平均菌落数为(12.37±20.76)CFU/cm2;细菌超标21副,超标率为40.38%;三类医务人员眼镜污染状况比较差异有统计学意义(P<0.05);眼镜携带细菌以表皮葡萄球菌为主;乙醇消毒棉签擦拭后,45副眼镜未检出细菌,7副眼镜检出细菌,眼镜染菌率为13.46%(7/52),但7副眼镜检出细菌菌落数均<5CFU/cm2。结论洁净手术室工作人员佩戴的眼镜污染严重,建议进入手术室前进行消毒处理。
OBJECTIVE To understand the status of carriage with bacteria in glasses of operating room staff so as to ensure the cleanliness of air in clean operating room and reduce the incidence of surgical incision infection.METHODS A questionnaire survey was conducted for the status of health care workers′wearing glasses,including surgeons,anesthesia staff and nurses who frequently entered clean operating room,the glasses of 52 participants were randomly sampled,the bacterial colony counts were calculated,the bacteria were identified,and the eradication rate of bacteria was detected after the treatment with ethyl alcohol.RESULTS Totally 180 health care workers who frequently entered in and out the clean operating room,including the surgeons,anesthesia staff and nurses,the total rate of wearing glasses was 56.67%(102/180).A total of 52 pairs of glasses were sampled for test,the highest bacterial colony counts were 108 CFU/cm~2,the mean bacterial colony counts were 12.37±20.76 CFU/cm~2.The bacterial colony counts of 21 pairs exceeded the standard,with the exceeded rate 40.38%;there was significant difference in the status of contamination of glasses among the three categories of health care workers(P<0.05).Staphylococcus aureus was the predominant species of bacteria isolated from the glasses;totally 45 pairs of glasses were not detected with bacteria after the disinfection with ethyl alcohol,7 pairs were detected with bacteria,with the bacterial contamination rate 13.46%(7/52),and the bacterial colony counts of the 7 pairs of glasses were less than 5 CFU/cm~2.CONCLUSIONThe glasses that are worn by the health care workers of the clean operating room are seriously contaminated,and it is suggested that the disinfection should be carried out before entering the operating room.
OBJECTIVE To understand the status of carriage with bacteria in glasses of operating room staff so as to ensure the cleanliness of air in clean operating room and reduce the incidence of surgical incision infection.METHODS A questionnaire survey was conducted for the status of health care workers′wearing glasses,including surgeons,anesthesia staff and nurses who frequently entered clean operating room,the glasses of 52 participants were randomly sampled,the bacterial colony counts were calculated,the bacteria were identified,and the eradication rate of bacteria was detected after the treatment with ethyl alcohol.RESULTS Totally 180 health care workers who frequently entered in and out the clean operating room,including the surgeons,anesthesia staff and nurses,the total rate of wearing glasses was 56.67%(102/180).A total of 52 pairs of glasses were sampled for test,the highest bacterial colony counts were 108 CFU/cm~2,the mean bacterial colony counts were 12.37±20.76 CFU/cm~2.The bacterial colony counts of 21 pairs exceeded the standard,with the exceeded rate 40.38%;there was significant difference in the status of contamination of glasses among the three categories of health care workers(P<0.05).Staphylococcus aureus was the predominant species of bacteria isolated from the glasses;totally 45 pairs of glasses were not detected with bacteria after the disinfection with ethyl alcohol,7 pairs were detected with bacteria,with the bacterial contamination rate 13.46%(7/52),and the bacterial colony counts of the 7 pairs of glasses were less than 5 CFU/cm~2.CONCLUSIONThe glasses that are worn by the health care workers of the clean operating room are seriously contaminated,and it is suggested that the disinfection should be carried out before entering the operating room.
引文
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[2]Fu Shaw L,Chen IH,Chen CS,et al.Factors influencing microbial colonies in the air of operating rooms[J].BMC Infect Dis,2018,18(1):4.
[3]Birgand G,Toupet G,Rukly S,et al.Air contamination for predicting wound contamination in clean surgery:a large multicenter study[J].Am J Infect Control,2015,3(5):516-521.
[4]许为全,曹莉.生物洁净室的污染控制[J].洁净与空调技术,2002(4):7-10.
[5]黄月娥,何丽萍.洁净手术室的规范化管理[J].临床合理用药,2017,10(3A):164-165.
[6]Association of periOperative Registered Nurses(AORN).Guidelines for perioperative practice[S].AORN,2016.
[7]中华人民共和国卫生部.医疗机构消毒技术规范WS/T 367-2012[S].中华人民共和国卫生部,2012.
[8]中华人民共和国卫生部.医院消毒卫生标准GB 15982-2012[S].中华人民共和国卫生部,2012.
[9]甘院梅,贾继芳.动态细菌学监测连台手术空气污染状况的调查研究[J].甘肃医药,2015,34(7):528-530.
[10]Kasina P,Tammelin A,Blomfeldt AM,et al.Comparison of three distinct clean air suits to decrease the bacterial load in the operating room:an observational study[J].Patient saf surg,2016,10(1):1-6.
[11]龚玉姣,冯文如,陈建东,等.棉织布和无纺布手术衣巾在洁净手术室铺单前后空气中尘埃粒子数比较[J].广东医学,2017,38(5):769-770.
[12]Gorny RL,awniczek A,Stobnicka WA,et al.Fibers as carriers ofmicrobial particles[J].Medycyna Pracy,2015,66(4):511-523.
[13]周春碚,朱兵,廖春艳,等.某市市级医院医务人员手机带菌状况与影响因素研究[J].中国感染控制杂志,2017,16(12):1141-1146.
[14]王秀梅.骨科植入物手术感染的手术室危险因素分析及预防对策[J].世界最新医学信息文摘,2017,17(76):178-180.