食品菌落总数测定盲样考核结果不确定度的评定
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摘要
目的评定食品菌落总数测定的不确定度。方法依据JJF 1059.1-2012《测定不确定度评定与表示》分析测定过程中引入的影响因素,建立数学模型,评定不确定度分量,按照均方根法则,将各分量不确定度进行加和,计算总测量不确定度。结果菌落总数测定结果的扩展不确定度为U=0.04854(以对数计),包含因子k=2(95%置信概率)。结论本文建立的方法模型可以对菌落总数盲样考核进行不确定度评定,此方法也适用于类似条件下菌落总数不确定度的评定。
Objective To evaluate the uncertainty of determination of aerobic plate count in food. Methods According to JJF 1059.1-2012 Evaluation and representation of measurement uncertainty, the influence factors introduced in the analysis and measurement process were analyzed, the mathematical model was established, and the uncertainty component was evaluated. According to the root-mean-square principle, measurement uncertainty components were added to calculate the total measurement uncertainty. Results Taking k=2(95% confidence level), the expanded uncertainty was 0.04854(in logarithmic). Conclusion The established method can be used in the uncertainty evaluation of aerobic plate count under similar circumstances.
Objective To evaluate the uncertainty of determination of aerobic plate count in food. Methods According to JJF 1059.1-2012 Evaluation and representation of measurement uncertainty, the influence factors introduced in the analysis and measurement process were analyzed, the mathematical model was established, and the uncertainty component was evaluated. According to the root-mean-square principle, measurement uncertainty components were added to calculate the total measurement uncertainty. Results Taking k=2(95% confidence level), the expanded uncertainty was 0.04854(in logarithmic). Conclusion The established method can be used in the uncertainty evaluation of aerobic plate count under similar circumstances.
引文
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[2]GB/T 277025-2008检测和校校准实验室能力的通通用要求[S].GB/T 277025-2008 General re equirements for the competence of testin ng and calibratio on laboratories[S].
[3]GB 47899.2-2016食品安全全国家标准食品微生生物学检验菌落总总数测定[S].GB 47789.2-2016 Nation nal food safety standard-Microbiol logical examinat tion in food-Aerobic c plate count[S].
[4]JJF 10599.1-2012测量不确定定度的评定与表示[[S].JJF 10559.1-2012 Evaluat tion and express ion of uncertaint ty in measurem ments[S].
[5]王海华,兰茜.能力验证菌菌落总数测定结果不不确定度的评定[J].食品安全质量量检测学报,2015,66(6):2352-2355.Wang HH,Lan Q.Uncert tainty evaluation off aerobic plate cou unt by proficien ncy testing[J].J Foo d Saf Qual,2015,6((6):2352-2355.
[6]吴环,聂聂炎炎,张慧,等.面面膜粉霉菌污染情况况分析及其灭菌总数数的不确定评定定[J].广东化工,20116,13(43):76.Wu H,NNie YY,Zhang H,et al.Investigation onn molds contaminatio on and the unce ertainty evaluation fo or molds enumeratioo n of facial mask p owder[J].Guan ngdong Chem Ind,2016,13(43):76.
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[9]Seppo IN.Uncee rtainty of quantit tative determination ns derived by cultivation of micrr oorganisms[EB/OLL].[2014-08-26].Fe nland,Helsinki:MIKES Publicationn J4/2003.http://ww ww.docin.com/P-7233331594.htmL
[10]JJG 196-2006常用用玻璃量器检定规程程[S]JJG 196-2006 Veri fication regulations f for locomotive pipet ttes[S].
[11]易大为,高敬红,邹宇.加权平均法法表述菌落总数测定定的数学模型与测量不确定度的评评定[J].食品安全质质量学报,2014,5(9):2888-2892.Yi DW,Gao JH,Zou Y.Establishm ment of mathemati ical model and evaluation of meas urement uncertainty y for aerobic plate co ount by weighted average[J].J Foodd Saf Qual,2014,5(99):2888-2892.
[12]JJG 1036-2008电子天平检定规程[S]].JJG 1036-2008 Elee ctronic balance[S].
[13]胡巅,贾松树,李李素芳,等.论菌落总总数测定(检测)的不不确定度及其评定[J].中国卫生检检查杂志,2015,25(66):761-764.Hu D,Ding W,Li SF,et al.Uncertaint ty evaluation for aer robic plate count of food[J].Chin JHealth Lab Technol,,2015,25(6):761-7764.
[14]武利平,张晓,丁培,等.能力验证菌菌落总数结果测量不不确定度评定分析[J].中国卫生检检验杂志,2017,27(224):3545-3547.Wu LP,Zhang X,Ding P,et al.Unc certainty evaluation and analysis of total bacterial counn t in proficiency test ting[J].Chin J Heal lth Lab Technol,2017,27(24):35455-3547.
[15]GB 4789.28-2013食品安全国家标准准食品微生物学检验培养基和制剂的质量要求[S]GB 4789.28-2013 National food safety standard--Microbiological examination in foo d-Quality requireme ents of medium and r reagents[S].
[2]GB/T 277025-2008检测和校校准实验室能力的通通用要求[S].GB/T 277025-2008 General re equirements for the competence of testin ng and calibratio on laboratories[S].
[3]GB 47899.2-2016食品安全全国家标准食品微生生物学检验菌落总总数测定[S].GB 47789.2-2016 Nation nal food safety standard-Microbiol logical examinat tion in food-Aerobic c plate count[S].
[4]JJF 10599.1-2012测量不确定定度的评定与表示[[S].JJF 10559.1-2012 Evaluat tion and express ion of uncertaint ty in measurem ments[S].
[5]王海华,兰茜.能力验证菌菌落总数测定结果不不确定度的评定[J].食品安全质量量检测学报,2015,66(6):2352-2355.Wang HH,Lan Q.Uncert tainty evaluation off aerobic plate cou unt by proficien ncy testing[J].J Foo d Saf Qual,2015,6((6):2352-2355.
[6]吴环,聂聂炎炎,张慧,等.面面膜粉霉菌污染情况况分析及其灭菌总数数的不确定评定定[J].广东化工,20116,13(43):76.Wu H,NNie YY,Zhang H,et al.Investigation onn molds contaminatio on and the unce ertainty evaluation fo or molds enumeratioo n of facial mask p owder[J].Guan ngdong Chem Ind,2016,13(43):76.
[7]CNAS-GL 05:20111测量不确定度的实实施指南[S].CNAS-GL 05:2011 Implementation n guideline of re quirements for measurement uncerr tainty[S].
[8]CNAS-CL 07:20111测量不确定度的要要求[S].CNAS-CL 07:20111Requirments for m measurement uncertai inty[S].
[9]Seppo IN.Uncee rtainty of quantit tative determination ns derived by cultivation of micrr oorganisms[EB/OLL].[2014-08-26].Fe nland,Helsinki:MIKES Publicationn J4/2003.http://ww ww.docin.com/P-7233331594.htmL
[10]JJG 196-2006常用用玻璃量器检定规程程[S]JJG 196-2006 Veri fication regulations f for locomotive pipet ttes[S].
[11]易大为,高敬红,邹宇.加权平均法法表述菌落总数测定定的数学模型与测量不确定度的评评定[J].食品安全质质量学报,2014,5(9):2888-2892.Yi DW,Gao JH,Zou Y.Establishm ment of mathemati ical model and evaluation of meas urement uncertainty y for aerobic plate co ount by weighted average[J].J Foodd Saf Qual,2014,5(99):2888-2892.
[12]JJG 1036-2008电子天平检定规程[S]].JJG 1036-2008 Elee ctronic balance[S].
[13]胡巅,贾松树,李李素芳,等.论菌落总总数测定(检测)的不不确定度及其评定[J].中国卫生检检查杂志,2015,25(66):761-764.Hu D,Ding W,Li SF,et al.Uncertaint ty evaluation for aer robic plate count of food[J].Chin JHealth Lab Technol,,2015,25(6):761-7764.
[14]武利平,张晓,丁培,等.能力验证菌菌落总数结果测量不不确定度评定分析[J].中国卫生检检验杂志,2017,27(224):3545-3547.Wu LP,Zhang X,Ding P,et al.Unc certainty evaluation and analysis of total bacterial counn t in proficiency test ting[J].Chin J Heal lth Lab Technol,2017,27(24):35455-3547.
[15]GB 4789.28-2013食品安全国家标准准食品微生物学检验培养基和制剂的质量要求[S]GB 4789.28-2013 National food safety standard--Microbiological examination in foo d-Quality requireme ents of medium and r reagents[S].