摘要
生物多样性的保护是人类生存和可持续发展的基础。虽然我们在全球物种多样性的保护方面已经做了大量工作,然而今天我们仍然面临着生态环境的恶化和物种灭绝速度加快的严峻局面。许多物种甚至在我们认识它们以前就消失了。为此我们必须加快研究步伐,建立更为科学和完善的种质资源搜集、保存和利用体系。本文简述了当前全球范围内植物种质资源的保存和利用现状,以及离体培养和超低温技术在植物种质资源保存上的应用,探讨了植物种质资源保存中存在的问题以及相应的解决策略。
引文
[1]叶创兴,廖文波,戴水连,李筱菊.植物学(系统分类部分).广州:中山大学出版社,2000.
[2]谷建田.中国植物种质资源保护:历史,现状与未来[J].科技导报,1994,6:59~62.
[3]Swaminathan MS.The past,present and future contributions of farmers to the conservation and development of genetic diversity.In:International conference on science and technology for managing plant genetic diversity in the 21st century[J].Engels JMM,Rao VR,Brown AHD,Jackson MT(eds.),CABI Publishing,New York,2000,pp.1~10.
[4]陈勇,竺杏月,张智光.自然保护区可持续发展研究的理论与方法评述[J].南京林业大学学报(自然科学版),2003,27(2):79~83.
[5]Scarascia~Mugnozza GT,Perrino P.The history of ex situ germplasm conservation and use of genetic resources.In:International conference on science and technology for managing plant genetic diversity in the 21st century[J].Engels JMM,Rao VR,Brown AHD,Jackson MT(eds.),CABIPublishing,New York,2000,11~28.
[6]Taylor M,Pone S,Palupe A.Slow growth strategies.In:In vitro conservation of plant genetic resources.Normah MN,Narimah MK,Clyde MM(eds.),Plant biotechnology laboratory,UKM,1996,119~134.
[7]Chandel KPS,Pandey R.Strategies for in vitro conservation of vegetative propagated species.In:In vitro conservation of plant genetic resources[J].Normah MN,Narimah MK,Clyde MM(eds.),Plant biotechnology laboratory,UKM,1996,193~202.
[8]Sakai A.Cryopreservation of plant genetic resources.In:Technical assistance activities for genetic resources projects.JICA,Tokyo,1993,pp.1~3.
[9]Withers LA.In vitro approaches to conversation of plant genetic resources.In:Plant tissue culture and its agricultural applications[J].Withers LA,Aldrrson PG(eds.),Butterworths,London,1986,261~276.
[10]Engelmann F.Alternative methods for the storage of recalcitrant seeds~an update.In:Recalcitrant seeds:Proceedings of the IUFRO seed symposium[J].Marzalina M,Khoo KC,Tsan FY,Krishnapillay B(eds.),Kuala Lumpur,Malaysia,1998,159~170.
[11]Engelmann F,Takai H(eds.).Cryopreservation of tropical plant germplasm:current research progress and application[J].JIRCAS,Tsukuba,2000.
[12]Withers LA.Low temperature storage of plant tissue cultures.In:Advances in biochemical engineering[J].Fiechler A(ed.),Springer Verlag,Berlin,1980,101~150.
[13]Luyet BJ.The vitrification of organic colloids and of protoplasm[J].Biodynamica,1937,1:1~14.
[14]Fahy GM,Takahashi T,Meryman HT.Practical aspects of ice~free cryopreservation.In:Future developments in blood banking[J].Sibinga CTS(ed.),Mastinus Nijhoff Publishing,Boston,1985,111~122.
[15]Engelmann F.Current research status and utilization of plant cryopreservation.In:Proceedings of the international workshop on cryopreservation of bio~genetic resources[J].Internation technical cooperation center rural development administration.Suwan,Republic of Korea,2003,19~41.
[16]Pistorisu R(ed.).Scientists,plants and politics~a history of the plant genetic resources movement[J].IPGRI,Rome,Italy,1997.