摘要
为研究中国典型湿地沉积物硝酸盐异化还原成铵过程(Dissimilatory nitrate reduction to ammonium,DNRA)的群落组成,针对DNRA过程的功能基因nrfA进行高通量测序.选取中国典型湿地岸边带的表层沉积物8个样点,质控后每个样品得到60000条序列,在相似度≥90%得到279个OTUs进行生态学分析.由基因丰度值显示:8个湿地沉积物的丰度为(6.69±0.28)×10~7~(8.44±0.48)×10~8 copies g~(-1).多样性分析(OTUs水平)结果表明:本研究的湿地沉积物样点中,南方湿地沉积物样点的多样性要高于北方样点.对代表OTUs进行分类,共定义到8个门(Phylum),23个属(Genus).其中相对丰度最高的3个属为Anaeromyxobacter(24.71%)、Anaerolinea(9.70%)和Dokdonella(7.94%),表明三者在群落组成中占主导地位.PCoA分析(OTUs水平)表明南北方地区差异是导致中国湿地沉积物中DNRA菌群结构不同的最主要影响因素.结合沉积物理化因子分析,DNRA细菌的丰度与碳氮比、年平均降水量及年平均温度呈显著正相关.本研究在一定程度上揭示了中国典型湿地沉积物DNRA细菌的群落组成、多样性及其与环境因子的关系.
To investigate the community structure of dissimilatory nitrate reduction to ammonium(DNRA) process in wetland sediments of China, high-throughput sequencing was carried out for the functional gene nrfA of DNRA bacteria. A total of 8 samples of surface sediments from typical wetlands in China were selected. After quality control, 60000 sequences were obtained for each sample, and 279 OTUs were obtained for community analysis at 90% sequence similarity. The abundance of DNRA bacteria ranged from(6.69±0.28)×10~7~(8.44±0.48)×10~8 copies·g~(-1). Results of diversity analysis(OTUs level) show that the diversity of wetland sediment samples in the south was higher than that in the north. The representative OTUs was classified into 8 phyla and 23 genera. The three highest richness genera were Anaeromyxobacter(24.71%), Anaerolinea(9.70%) and Dokdonella(7.94%), indicating their dominant role in DNRA community composition. PCoA analysis(OTUs level) shows that the difference between the north and the south was the main factor leading to the structure of DNRA bacteria in wetland sediments. The abundance of DNRA bacteria was positively correlated with C/N, annual average precipitation and annual average temperature. in summary, this study reveals the community structure and diversity of DNRA bacteria in typical wetland sediments of China and their relationship with environmental factors.
引文
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