采用分子生物学技术诊断隐球菌脑膜脑炎的研究
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摘要
目的采用分子检测技术对疑似隐球菌感染的脑膜炎病例进行诊断。方法收集患者的脑脊液样本,提取DNA,设计引物进行PCR扩增,采用DNA芯片技术对扩增产物进行分子检测。结果显示样本新生隐球菌阳性。结论通过ITS保守序列设计引物进行PCR扩增和DNA芯片技术对常规真菌学检查不能确定的疑似隐球菌脑膜炎患者脑脊液样本进行非培养检测,具有实验室诊断参考价值。
Objective To diagnose the case of meningitis suspected of cryptococcal infection by molecular detection technique. Methods Cerebrospinal fluid samples of patients were collected, DNA was extracted, primers were designed for PCR amplification, and gene detection was performed on the amplified products by DNA chip technology. Results Sample showed positive results for Cryptococcus neoformans. Conclusion PCR amplification of primers designed from ITS conserved sequence and non-culture detection of cerebrospinal fluid samples by DNA chip technology have clinical reference value for deep fungal infections that are difficult to diagnose by traditional methods.
Objective To diagnose the case of meningitis suspected of cryptococcal infection by molecular detection technique. Methods Cerebrospinal fluid samples of patients were collected, DNA was extracted, primers were designed for PCR amplification, and gene detection was performed on the amplified products by DNA chip technology. Results Sample showed positive results for Cryptococcus neoformans. Conclusion PCR amplification of primers designed from ITS conserved sequence and non-culture detection of cerebrospinal fluid samples by DNA chip technology have clinical reference value for deep fungal infections that are difficult to diagnose by traditional methods.
引文
[1] 黄奕江.侵袭性真菌感染的流行病学及其高危因素[J].临床内科杂志,2008,25(4):221-223.
[2] 扈容英.医院内侵袭性真菌感染的前瞻性研究[D].宁夏医科大学,2013.
[3] 刘加.基于核酸等温扩增技术的克柔念珠菌早期分子诊断技术[D].第二军医大学,2017.
[4] 姚丽敏.五例真菌性鼻窦炎病例报道及相关实验研究[C].第六届中国临床微生物学大会暨微生物学与免疫学论坛论文汇编.中国微生物学会临床微生物学专业委员会、医学参考报社、宁波大学医学院附属医院,2015:1.
[5] 谈燕.PCR快速鉴定皮肤癣菌病[D].上海:第二军医大学,2014.
[6] 李娟.艾滋病常见真菌性机会感染基因芯片筛查试剂盒的研制[D].河南:河南中医学院,2014.
[7] Martins Mdos A,Brighente KB,Matos TA,et al.Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid:comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex[J].Braz J Infect Dis,19(1):62-67.
[8] Rivera V,Gaviria M,Mu C,et al.Validation and clinical application of a molecular method for the identification of Cryptococcus neoformans/Cryptococcus gattii complex DNA in human clinical specimens[J].Braz J Infect Dis,19(6):563-570.
[9] 杨英.新发及罕见侵袭性真菌病原体的基因组研究及分子诊断[D].北京:中国人民解放军军事医学科学院,2017.
[2] 扈容英.医院内侵袭性真菌感染的前瞻性研究[D].宁夏医科大学,2013.
[3] 刘加.基于核酸等温扩增技术的克柔念珠菌早期分子诊断技术[D].第二军医大学,2017.
[4] 姚丽敏.五例真菌性鼻窦炎病例报道及相关实验研究[C].第六届中国临床微生物学大会暨微生物学与免疫学论坛论文汇编.中国微生物学会临床微生物学专业委员会、医学参考报社、宁波大学医学院附属医院,2015:1.
[5] 谈燕.PCR快速鉴定皮肤癣菌病[D].上海:第二军医大学,2014.
[6] 李娟.艾滋病常见真菌性机会感染基因芯片筛查试剂盒的研制[D].河南:河南中医学院,2014.
[7] Martins Mdos A,Brighente KB,Matos TA,et al.Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid:comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex[J].Braz J Infect Dis,19(1):62-67.
[8] Rivera V,Gaviria M,Mu C,et al.Validation and clinical application of a molecular method for the identification of Cryptococcus neoformans/Cryptococcus gattii complex DNA in human clinical specimens[J].Braz J Infect Dis,19(6):563-570.
[9] 杨英.新发及罕见侵袭性真菌病原体的基因组研究及分子诊断[D].北京:中国人民解放军军事医学科学院,2017.