反复呼吸道感染瘀热内结证儿童的免疫功能特点
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摘要
目的观察反复呼吸道感染(RRTI)瘀热内结证患儿血清中细胞免疫因子的变化,了解患儿机体的免疫功能状态。方法采用ELISA双抗体夹心法对50例RRTI患儿及50例健康儿童血清中的IFN-γ、IL-4、IL-10、IL-12进行检测。结果 RRTI患儿血清中IL-4、IL-10、IL-12较健康组儿童明显上升(P <0. 01),IFN-γ、IL-4、IL-10、IL-12之间无直线相关关系(P> 0. 05)。结论 RRTI瘀热内结证患儿发病时出现免疫失衡,表现为Th1类因子及Th2类因子表达均上调。
Objective To observe the changes of cellular immune factors in serum of children with recurrent respiratory tract infection of blood stasis and heat accumulation syndrome,and to understand the immune function status of the children's body. Methods The serum IFN-γ,IL-4,IL-10,IL-12 in 50 children with RRTI and 50 healthy children was detected by double antibody sandwich enzyme-linked immune sorbent assay( ELISA) method. Results The levels of IL-4,IL-10,IL-12 in serum of children with RRTI were significantly higher than those in normal group( P < 0. 01),and there was no linear correlation between IL-4,IL-10,IL-12 and IFN-γ( P > 0. 05). Conclusions There is immune imbalance in children with RRTI syndrome of blood stasis and heat endodontic,both Th1 type factor and Th2 type factor are increased.
Objective To observe the changes of cellular immune factors in serum of children with recurrent respiratory tract infection of blood stasis and heat accumulation syndrome,and to understand the immune function status of the children's body. Methods The serum IFN-γ,IL-4,IL-10,IL-12 in 50 children with RRTI and 50 healthy children was detected by double antibody sandwich enzyme-linked immune sorbent assay( ELISA) method. Results The levels of IL-4,IL-10,IL-12 in serum of children with RRTI were significantly higher than those in normal group( P < 0. 01),and there was no linear correlation between IL-4,IL-10,IL-12 and IFN-γ( P > 0. 05). Conclusions There is immune imbalance in children with RRTI syndrome of blood stasis and heat endodontic,both Th1 type factor and Th2 type factor are increased.
引文
[1]中华医学会儿科学分会呼吸学组,《中华儿科杂志》编辑委员会.反复呼吸道感染的概念和处理原则[J].中华儿科杂志,2008,46(2):108-109.
[2]霍莉莉,虞坚尔,李利清,等.小儿反复呼吸道感染发病机制的中西医研究[J].中医儿科杂志,2006,2(3):45-49.
[3]崔霞,齐智星,王倩,等.从瘀热论治儿童反复呼吸道感染[C]//中华中医药学会儿科分会第三十次学术大会论文集.济南:中华中医药学会儿科分会第三十次学术大会,2013:236-237.
[4]王静,崔霞,王坤,等.当归六黄汤对瘀热内结模型小鼠Th1/Th2细胞因子的影响[J].世界中西医结合杂志,2016,11(4):503-505,539.
[5]周仲瑛,吴勉华,周学平.瘀热相搏证中医辨治指南[J].中华中医药杂志,2010,25(9):1411-1414.
[6]王坤.当归六黄汤治疗小儿反复呼吸道感染瘀热内结证的临床疗效观察[D].北京:北京中医药大学,2014.
[7]游金勇.小儿反复呼吸道感染中医证型研究的文献计量分析[J].广西中医药,2005,28(1):51-53.
[8]王力宁,汪受传,韩新民,等.《小儿反复呼吸道感染中医诊疗指南》指标的Delphi法评价与结果分析[J].中国中西医结合儿科学,2009,1(1):42-47.
[9]周静冬.虞坚尔教授治疗小儿反复呼吸道感染临床经验[J].南京中医药大学学报,2014,30(6):583-584.
[10]孟伟霞.小儿气虚血瘀型反复呼吸道感染证治与儿童气虚质相关性研究探讨[D].北京:北京中医药大学,2012.
[11]王明香,陈诗鸿,张桂菊,等.益气活血法防治小儿反复呼吸道感染的临床研究[J].山东中医杂志,2000,19(3):136-137.
[12]董斐,谷晓红.从胃肠积热论治小儿反复呼吸道感染[C]//第三次全国温病学论坛暨温病学辨治思路临床拓展应用高级研修班论文集.枣庄:第三次全国温病学论坛暨温病学辨治思路临床拓展应用高级研修班,2016:6.
[13]马融,李新民,于越,等.小儿反复呼吸道感染证候规律及中医药治疗方案规范研究[J].天津中医药,2012,29(5):519.
[14]崔霞,王素梅,吴力群,等.刘弼臣应用当归六黄汤治疗儿科常见病举隅[J].辽宁中医杂志,2010,37(4):735-736.
[15]刘云锋,陈秉孟,关克涛,等.反复呼吸道感染儿童血清中白介素2,4,8及受体2的检测[J].广东医学,2005,26(1):74-75.
[16]王丽英,周其刚,张磊磊.脾氨肽对反复呼吸道感染患儿Th1/Th2细胞因子的影响[J].中国全科医学,2009,12(6):489-490.
[17]BACH JF.The effect of infectious on susceptibility to autoimmune and allergic diseases[J].N Eng J Med,2002,347(12):930-931.
[18]张艳丽,王秀芳,雷瑞瑞,等.哮喘、毛细支气管炎患儿外周血MDSCs、IL-10和IL-12水平及意义[J].西安交通大学学报(医学版),2013,34(4):503-507,550.
[19]KIDD P.Th1/Th2 balance:the hypothesis,its limitations,and limitations for health and disease[J].Alternative Medicine Review,2003,8(3):223-246.
[20]SEALANT S,DUVERTt V,MOREAU I,et al.Human Bcell precursors proliferate and express CD23 after CD40ligation[J].Exp Med,1993,178:113-20.
[21]王颖华.反复呼吸道感染患儿血清T细胞亚群及相关细胞因子水平的变化[J].中国基层医药,2009,16(3):429-430.
[22]王琦,李红,王宁萍,等.反复呼吸道感染患儿免疫球蛋白及相关细胞因子水平分析[J].宁夏医学院学报,2007,29(5):451-453.
[23]白井林,刘薇薇,张同园.培土生金法对脾虚证反复呼吸道感染小鼠免疫功能的影响[J].中国中医急症,2015,24(2):281-282.
[2]霍莉莉,虞坚尔,李利清,等.小儿反复呼吸道感染发病机制的中西医研究[J].中医儿科杂志,2006,2(3):45-49.
[3]崔霞,齐智星,王倩,等.从瘀热论治儿童反复呼吸道感染[C]//中华中医药学会儿科分会第三十次学术大会论文集.济南:中华中医药学会儿科分会第三十次学术大会,2013:236-237.
[4]王静,崔霞,王坤,等.当归六黄汤对瘀热内结模型小鼠Th1/Th2细胞因子的影响[J].世界中西医结合杂志,2016,11(4):503-505,539.
[5]周仲瑛,吴勉华,周学平.瘀热相搏证中医辨治指南[J].中华中医药杂志,2010,25(9):1411-1414.
[6]王坤.当归六黄汤治疗小儿反复呼吸道感染瘀热内结证的临床疗效观察[D].北京:北京中医药大学,2014.
[7]游金勇.小儿反复呼吸道感染中医证型研究的文献计量分析[J].广西中医药,2005,28(1):51-53.
[8]王力宁,汪受传,韩新民,等.《小儿反复呼吸道感染中医诊疗指南》指标的Delphi法评价与结果分析[J].中国中西医结合儿科学,2009,1(1):42-47.
[9]周静冬.虞坚尔教授治疗小儿反复呼吸道感染临床经验[J].南京中医药大学学报,2014,30(6):583-584.
[10]孟伟霞.小儿气虚血瘀型反复呼吸道感染证治与儿童气虚质相关性研究探讨[D].北京:北京中医药大学,2012.
[11]王明香,陈诗鸿,张桂菊,等.益气活血法防治小儿反复呼吸道感染的临床研究[J].山东中医杂志,2000,19(3):136-137.
[12]董斐,谷晓红.从胃肠积热论治小儿反复呼吸道感染[C]//第三次全国温病学论坛暨温病学辨治思路临床拓展应用高级研修班论文集.枣庄:第三次全国温病学论坛暨温病学辨治思路临床拓展应用高级研修班,2016:6.
[13]马融,李新民,于越,等.小儿反复呼吸道感染证候规律及中医药治疗方案规范研究[J].天津中医药,2012,29(5):519.
[14]崔霞,王素梅,吴力群,等.刘弼臣应用当归六黄汤治疗儿科常见病举隅[J].辽宁中医杂志,2010,37(4):735-736.
[15]刘云锋,陈秉孟,关克涛,等.反复呼吸道感染儿童血清中白介素2,4,8及受体2的检测[J].广东医学,2005,26(1):74-75.
[16]王丽英,周其刚,张磊磊.脾氨肽对反复呼吸道感染患儿Th1/Th2细胞因子的影响[J].中国全科医学,2009,12(6):489-490.
[17]BACH JF.The effect of infectious on susceptibility to autoimmune and allergic diseases[J].N Eng J Med,2002,347(12):930-931.
[18]张艳丽,王秀芳,雷瑞瑞,等.哮喘、毛细支气管炎患儿外周血MDSCs、IL-10和IL-12水平及意义[J].西安交通大学学报(医学版),2013,34(4):503-507,550.
[19]KIDD P.Th1/Th2 balance:the hypothesis,its limitations,and limitations for health and disease[J].Alternative Medicine Review,2003,8(3):223-246.
[20]SEALANT S,DUVERTt V,MOREAU I,et al.Human Bcell precursors proliferate and express CD23 after CD40ligation[J].Exp Med,1993,178:113-20.
[21]王颖华.反复呼吸道感染患儿血清T细胞亚群及相关细胞因子水平的变化[J].中国基层医药,2009,16(3):429-430.
[22]王琦,李红,王宁萍,等.反复呼吸道感染患儿免疫球蛋白及相关细胞因子水平分析[J].宁夏医学院学报,2007,29(5):451-453.
[23]白井林,刘薇薇,张同园.培土生金法对脾虚证反复呼吸道感染小鼠免疫功能的影响[J].中国中医急症,2015,24(2):281-282.