超声造影定量分析L-精氨酸改善大鼠肾缺血再灌注损伤的应用价值
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摘要
目的应用超声造影技术定量分析L-精氨酸对大鼠肾缺血再灌注损伤肾皮质血流灌注的影响,探讨超声造影技术在评价药物干预肾缺血再灌注损伤疗效中的应用价值。方法健康雄性大鼠24只随机分成假手术组(Sham组)、缺血再灌损伤组(I/R组)和L-精氨酸组(L-arg组),每组8只。行超声造影检查观察肾皮质血流灌注情况并脱机分析时间-强度曲线(TIC)。实验结束后,行肾组织病理学检查。结果与假手术组比较,I/R组和L-arg组肾脏病理改变明显,达峰时间(TTP)和平均通过时间(MTT)延长,曲线下面积(AUC)显著增加(P<0.05);与I/R组比较,L-arg组病理改变显著,峰值浓度(Peak)增高,TTP、MTT缩短,AUC减少(P<0.05)。结论超声造影结合时间-强度曲线能定量分析L-精氨酸对大鼠肾缺血再灌注损伤肾皮质血流灌注的改善。
Objective The application of contrast-enhanced ultrasound to quantitatively analyze the effect of L-arginine on renal cortex blood perfusion in rats with renal ischemia-reperfusion injury,and to evaluate the value of contrast-enhanced ultrasound in the evaluation of drug intervention in renal ischemia-reperfusion injury.Methods Twenty-four healthy male rats were randomly divided into sham-operated group(Sham group),ischemia-reperfusion injury group(I/R group)and L-arginine group(L-arg group),with 8 rats in each group.Contrast-enhanced ultrasound was performed to observe the renal cortex blood flow perfusion and the time-intensity curve(TIC)was analyzed off-line.At the end of the experiment,renal histopathological examinations were performed.Results Compared with the sham-operated group,the pathological changes of the kidney in the I/R group and the L-arg group were significant,the peak time(TTP)and mean transit time(MTT)were prolonged,and the area under the curve(AUC)was significantly increased(P<0.05);Compared with the I/R group,the pathological changes in the L-arg group were significant,Peak increased,TTP,MTT shortened,and AUC decreased(P<0.05).Conclusions Contrast-enhanced ultrasound combined with time-intensity curve can quantitatively analyze the improvement of renal cortical blood flow in rats with renal ischemia-reperfusion injury induced by L-arginine.
Objective The application of contrast-enhanced ultrasound to quantitatively analyze the effect of L-arginine on renal cortex blood perfusion in rats with renal ischemia-reperfusion injury,and to evaluate the value of contrast-enhanced ultrasound in the evaluation of drug intervention in renal ischemia-reperfusion injury.Methods Twenty-four healthy male rats were randomly divided into sham-operated group(Sham group),ischemia-reperfusion injury group(I/R group)and L-arginine group(L-arg group),with 8 rats in each group.Contrast-enhanced ultrasound was performed to observe the renal cortex blood flow perfusion and the time-intensity curve(TIC)was analyzed off-line.At the end of the experiment,renal histopathological examinations were performed.Results Compared with the sham-operated group,the pathological changes of the kidney in the I/R group and the L-arg group were significant,the peak time(TTP)and mean transit time(MTT)were prolonged,and the area under the curve(AUC)was significantly increased(P<0.05);Compared with the I/R group,the pathological changes in the L-arg group were significant,Peak increased,TTP,MTT shortened,and AUC decreased(P<0.05).Conclusions Contrast-enhanced ultrasound combined with time-intensity curve can quantitatively analyze the improvement of renal cortical blood flow in rats with renal ischemia-reperfusion injury induced by L-arginine.
引文
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[9] COSTA W S,RIBEIRO M N,CARDOSO L E,et al.Nutritional supplementation with L-arginine prevents pelvic radiationinduced changes in morphology,density,and regulating factors of blood vessels in the wall of rat bladder[J].World journal of urology,2013,31(3):653-658.
[2]徐婷,马龙,王毅,等.miRNA-21和左旋精氨酸在大鼠肾缺血再灌注损伤中的作用[J].新疆医科大学学报,2018,41(2):203-207.
[3] TONG F,ZHOU X.The Nrf2/HO-1Pathway Mediates the Antagonist Effect of L-Arginine On Renal Ischemia/Reperfusion Injury in Rats[J].Kidney blood pressure research,2017,42(3):519-529.
[4] MAHONEY M,SORACE A,WARRAM J,et al.Volumetric contrast-enhanced ultrasound imaging of renal perfusion[J].Journal of ultrasound in medicine,2014,33(8):1427-1437.
[5] SI Y N,HAN L,ZHANG Y,et al.Effects of dexmedetomidine on microcirculatory perfusion in rabbits with renal ischemia/reperfusion injury:quantitative evaluation with contrast-enhanced ultrasound[J].Journal of Southern Medical University,2016,36(5):628-632.
[6]李明星,叶帆,罗志建,等.超声造影定量评价肾缺血再灌注损伤后兔肾皮质血流动力学改变[J].四川医学,2013,34(12):1769-1771.
[7]SCHWEIGER H,OHLERTH S,GERBER B.Contrast-enhanced ultrasound of both kidneys in healthy,non-anaesthetized cats[J].Acta veterinaria Scandinavica,2015,57(11):80.
[8]叶帆,李明星,罗志建,等.超声造影监测兔肾缺血再灌注损伤早期皮质血流动力学的改变[J/OL].中华临床医师杂志:电子版,2015,9(6):62-65.
[9] COSTA W S,RIBEIRO M N,CARDOSO L E,et al.Nutritional supplementation with L-arginine prevents pelvic radiationinduced changes in morphology,density,and regulating factors of blood vessels in the wall of rat bladder[J].World journal of urology,2013,31(3):653-658.