温度对肝片形吸虫在小土锅螺体内发育影响的观察
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摘要
目的探讨温度对肝片形吸虫毛蚴感染小土蜗螺及毛蚴在其体内发育的影响,以期了解片形吸虫的生物学特征,为防控肝片形吸虫病提供科学依据。方法从云南省大理市下关泰兴市场收集病牛肝胆管及胆囊内的肝片形吸虫虫体并剥离出虫卵,孵化出毛蚴,在实验室分批进行人工感染小土蜗螺并观察不同温度下螺内虫体的发育状况。结果平均水温10℃时肝片形吸虫幼虫在小土蜗螺体内只能发育到胞蚴阶段,12℃时只能发育到母雷蚴阶段,18℃时只能发育到子雷蚴早期阶段,22℃时发育到尾蚴阶段。结论温度是影响肝片形吸虫幼虫在小土蜗螺体内发育的重要因素。
Objective To explore the influence of temperature on the miracidium of infection Galba pervia and miracidium in its development,and to understand the biological characteristics of the fasciola hepatica. Methods hepatica eggs from the hepatic duct and gallbladder of cattle were collected from the shimonoseki taixing market of Dali city,miracidia were incubated and Galba pervia were infected with F. hepatica in the laboratory. Results Under average temperature 10 ℃,the shape of liver piece trematode larvae in small soil worm screw could only develop to cell larva stage; under 12 ℃ it developed to the mother redia stage; under 18 ℃it only developed to early daughter redia,and in 22 ℃ it developed to the larvae stage. Conclusion The temperature is a key factor to infect the development of F. hepatica larvae.
Objective To explore the influence of temperature on the miracidium of infection Galba pervia and miracidium in its development,and to understand the biological characteristics of the fasciola hepatica. Methods hepatica eggs from the hepatic duct and gallbladder of cattle were collected from the shimonoseki taixing market of Dali city,miracidia were incubated and Galba pervia were infected with F. hepatica in the laboratory. Results Under average temperature 10 ℃,the shape of liver piece trematode larvae in small soil worm screw could only develop to cell larva stage; under 12 ℃ it developed to the mother redia stage; under 18 ℃it only developed to early daughter redia,and in 22 ℃ it developed to the larvae stage. Conclusion The temperature is a key factor to infect the development of F. hepatica larvae.
引文
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[2]王映琴.云南省漾濞县牛羊肝片形吸虫病的查治[J].中国兽医寄生虫病,2007,15(2):27-28.
[3]胡小九,李石友,李文学,等.弥渡县牛肝片吸虫病流行调查[J].西南农业学报,2012,25(1):286-289.
[4]吕红生.祥云县羊肝片形吸虫病及防治效果调查[J].云南畜牧兽医,1998,(2):17.
[5]黄正美,刑兰燕,李六九,等.肝片吸虫病2例报告[J].寄生虫病与感染性疾病,2009,7(3):172.
[6]陈木新,艾琳,许学年,等.云南省大理州大片形吸虫群体感染26例分析[J].中国地方病学杂志,2012,31(6):595-598.
[7]Chen JX,Chen MX,Ai L,et al.An Outbreak of human fascioliasis gigantica in southwest China[J].PLo S One,2013,8(8):e71520.
[8]才项吉.硝氯粉和肝蛭净驱除绵羊肝片吸虫效果的对比试验,青海畜牧兽医杂志[J],2012,42(3):7-8.
[9]闵正沛,李军,黎志强.肝片形吸虫囊蚴培育及其中间宿主的研究[J].中国兽医杂志,1997,23(10):6-7.
[10]王冬英.广西片形吸虫流行情况,在中间宿主体内的发育与分类学的初步研究[D].广西:广西大学,2006.
[11]林宇光,孙毓兰,陈存瑞,等.大片吸虫的发育史和二种片形吸虫的流行病学研究[J].动物学报,1974,20(4):378-390.
[12]方文,李天美,李科荣,等.大理地区肝片形吸虫感染中间宿主实验研究[J].中国寄生虫学与寄生虫病杂志,2014,32(4):285-288.