水稻核不育突变体gsl5花粉壁单糖组分研究
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摘要
水稻花粉壁在花粉发育、适应环境和授粉识别过程中具有重要作用。花粉壁的发育异常会导致水稻雄性不育。在水稻花粉壁发育过程中,胼胝质层形成、初生外壁沉积和孢粉素的生物合成是关键步骤。目前对花粉初生外壁胼胝质层的研究较多,但对于水稻花粉壁的单糖组分缺乏整体性研究。我们利用花粉胼胝质层缺失的水稻突变体gsl5与野生型进行比较,对花粉壁单糖组分进行了色谱分析,初步研究了胼胝质层缺失对水稻花粉外壁组分的影响。
Pollen wall plays vital role during microspore development and pollination in rice(Oryza Japonica). Abnormal pollen wallformation usually leads to male sterility. Callose wall,microspore primary wall development and sporonpollenin accumulation are key step tocontrol pollen wall formation. Recent studies focus on callose wall formation during microspore development. Neither less,the knowledge aboutmonosaccharide composition in the pollen wall is limited. In this study,we compared the monosaccharide composition in the pollen wall of thewide type rice with a male sterility mutant gsl5.
Pollen wall plays vital role during microspore development and pollination in rice(Oryza Japonica). Abnormal pollen wallformation usually leads to male sterility. Callose wall,microspore primary wall development and sporonpollenin accumulation are key step tocontrol pollen wall formation. Recent studies focus on callose wall formation during microspore development. Neither less,the knowledge aboutmonosaccharide composition in the pollen wall is limited. In this study,we compared the monosaccharide composition in the pollen wall of thewide type rice with a male sterility mutant gsl5.
引文
[1]李仕贵,周开达,朱立煌.水稻温敏显性核不育基因的遗传分析和分子标记定位[J].科学通报,1999,44(9):955.
[2]袁隆平.水稻光,温敏不育系的提纯和原种生产[J].杂交水稻,1994(6):1-3.
[3]Ariizumi T,Toriyama K.Genetic regulation of sporopolleninsynthesis and pollen exine development[J].Annual Review ofPlant Biology,2012,62(1):437.
[4]Lu P,Chai M,Yang J,et al.The Arabidopsis CALLOSE DEFECTIVEMICROSPORE1 gene is required for male fertility throughregulating callose metabolism during microsporogenesis.[J].PlantPhysiology,2014,164(4):1893.
[5]Worrall D,Hird DL,Hodge R,et al.Premature dissolution of themicrosporocyte callose wall causes male sterility in transgenictobacco[J].Plant Cell,1992,4(7):759-71.
[6]Dong X,Hong Z,Sivaramakrishnan M,et al.Callose synthase(Cal S5)is required for exine formation during microgametogenesisand for pollen viability in Arabidopsis[J].Plant Journal for Cell&Molecular Biology,2005,42(3):315-328.
[7]Enns LC,Kanaoka MM,Torii KU,et al.Two callose synthases,GSL1and GSL5,play an essential and redundant role in plant and pollendevelopment and in fertility[J].Plant Molecular Biology,2005,58(3):333-349.
[8]Nishikawa S,Zinkl GM,Swanson RJ,et al.Callose(β-1,3 glucan)is essential for Arabidopsis pollen wall patterning,but not tubegrowth[J].Bmc Plant Biology,2005,5(1):1-9.
[9]Shi X,Han X,Lu T.Callose synthesis during reproductive development in monocotyledonous and dicotyledonous plants[J].PlantSignaling&Behavior,2015,11(2).
[10]Shi X,Sun X,Zhang Z,et al.GLUCAN SYNTHASE-LIKE 5(GSL5)plays an essential role in male fertility by regulatingcallose metabolism during microsporogenesis in rice[J].Plant&Cell Physiology,2015,56(3):497.
[11]Yang C,Zhou H,Shi X,et al.Identification and Analysis of Zbs1,aDominant Male-Sterile Mutant of Naked Oat(Avena nuda L.)[J].2016,56(4).
[12]York WS,Darvill AG,Mcneil M,et al.Isolation and characterization of plant cell walls and cell wall components[J].Methods inEnzymology,1986,118(1):3-40.
[2]袁隆平.水稻光,温敏不育系的提纯和原种生产[J].杂交水稻,1994(6):1-3.
[3]Ariizumi T,Toriyama K.Genetic regulation of sporopolleninsynthesis and pollen exine development[J].Annual Review ofPlant Biology,2012,62(1):437.
[4]Lu P,Chai M,Yang J,et al.The Arabidopsis CALLOSE DEFECTIVEMICROSPORE1 gene is required for male fertility throughregulating callose metabolism during microsporogenesis.[J].PlantPhysiology,2014,164(4):1893.
[5]Worrall D,Hird DL,Hodge R,et al.Premature dissolution of themicrosporocyte callose wall causes male sterility in transgenictobacco[J].Plant Cell,1992,4(7):759-71.
[6]Dong X,Hong Z,Sivaramakrishnan M,et al.Callose synthase(Cal S5)is required for exine formation during microgametogenesisand for pollen viability in Arabidopsis[J].Plant Journal for Cell&Molecular Biology,2005,42(3):315-328.
[7]Enns LC,Kanaoka MM,Torii KU,et al.Two callose synthases,GSL1and GSL5,play an essential and redundant role in plant and pollendevelopment and in fertility[J].Plant Molecular Biology,2005,58(3):333-349.
[8]Nishikawa S,Zinkl GM,Swanson RJ,et al.Callose(β-1,3 glucan)is essential for Arabidopsis pollen wall patterning,but not tubegrowth[J].Bmc Plant Biology,2005,5(1):1-9.
[9]Shi X,Han X,Lu T.Callose synthesis during reproductive development in monocotyledonous and dicotyledonous plants[J].PlantSignaling&Behavior,2015,11(2).
[10]Shi X,Sun X,Zhang Z,et al.GLUCAN SYNTHASE-LIKE 5(GSL5)plays an essential role in male fertility by regulatingcallose metabolism during microsporogenesis in rice[J].Plant&Cell Physiology,2015,56(3):497.
[11]Yang C,Zhou H,Shi X,et al.Identification and Analysis of Zbs1,aDominant Male-Sterile Mutant of Naked Oat(Avena nuda L.)[J].2016,56(4).
[12]York WS,Darvill AG,Mcneil M,et al.Isolation and characterization of plant cell walls and cell wall components[J].Methods inEnzymology,1986,118(1):3-40.