大蒜微型鳞茎诱导培养基的筛选初报
|
摘要
以吉木萨尔白蒜为试验材料,利用大蒜鳞茎盘为外植体,在B5+6-BA0.5 mg/L+NAA0.5 mg/L+蔗糖30 g/L+琼脂4.5 g/L培养基进行初始培养获得绿芽,在此基础上开展试管内诱导微鳞茎培养基配方研究。结果表明,1/2 MS+IBA 1.5 mg/L+NAA 0.1 gm/L+蔗糖50 g/L+琼脂4.5 g/L为组培苗试管内诱导鳞茎较好的培养基配方,在该培养基上大蒜绿芽第45天开始形成鳞茎,第90天鳞茎直径为0.3~0.5 cm。
The Jimsar white garlic as experimental materials,using garlic bulb as explants,Initial culture is carried out to obtain green bud in B5+6-BA 0.5 mg/ L +NAA0.5 mg/L + sucrose 30 g/L + agar 4.5 g/L medium,on the basis of this study,the formula of the culture medium for inducing the micro bulb in vitro is developed. The result shows that 1/2 MS +IBA 1.5 mg/L+NAA0.1 gm/L 1/2 MS + IBA 1.5 mg/L + sugar + 50 g/L + agar 4.5 g/L is a better culture medium formula for inducing bulb in tissue culture plantlet,the garlic green shoots began forming a bulb the in 45 th day,the bulb diameter up to 0.3 ~0.5 cm in 90 th day under the culture medium.
The Jimsar white garlic as experimental materials,using garlic bulb as explants,Initial culture is carried out to obtain green bud in B5+6-BA 0.5 mg/ L +NAA0.5 mg/L + sucrose 30 g/L + agar 4.5 g/L medium,on the basis of this study,the formula of the culture medium for inducing the micro bulb in vitro is developed. The result shows that 1/2 MS +IBA 1.5 mg/L+NAA0.1 gm/L 1/2 MS + IBA 1.5 mg/L + sugar + 50 g/L + agar 4.5 g/L is a better culture medium formula for inducing bulb in tissue culture plantlet,the garlic green shoots began forming a bulb the in 45 th day,the bulb diameter up to 0.3 ~0.5 cm in 90 th day under the culture medium.
引文
[1]曹孜义,刘国民.实用植物组织培养技术教程[M].兰州:甘肃科学技术出版社,1996:286-290.
[2]王蒂,陈劲枫.植物组织培养[M].北京:中国农业出版社,2013:237-241.
[3]李小川,赵美华.大蒜鳞芽生长点离体培养诱导小鳞茎的形成[J].山西农业科学,1996(3):59-60.
[4]孔素萍,段乃彬,等.大蒜鳞茎盘培养诱导簇生芽和微鳞茎.天津农业科学,2010(2):152.
[5]梁艳.大蒜试管苗鳞茎化培养及内源激素的变化[D].哈尔滨:东北农业大学,2005.
[2]王蒂,陈劲枫.植物组织培养[M].北京:中国农业出版社,2013:237-241.
[3]李小川,赵美华.大蒜鳞芽生长点离体培养诱导小鳞茎的形成[J].山西农业科学,1996(3):59-60.
[4]孔素萍,段乃彬,等.大蒜鳞茎盘培养诱导簇生芽和微鳞茎.天津农业科学,2010(2):152.
[5]梁艳.大蒜试管苗鳞茎化培养及内源激素的变化[D].哈尔滨:东北农业大学,2005.