植物小分子信号肽CIF对拟南芥矿质元素吸收和积累的影响
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摘要
通过人工合成植物小分子信号肽CIF (Casparian strip integrity factor)核心序列,并用其处理拟南芥根和叶,探讨CIF对拟南芥中矿质元素吸收和积累的影响。结果表明:根施0.1μmol·L~(-1)的CIF可以显著促进根中镁、钙、锰、铁、锌、铜和硼等矿质元素的积累;叶施CIF可显著提高根中镁、钙、锰、铁、锌和铜等元素的含量,而对拟南芥叶中矿质元素含量影响较小。CIF可以显著诱导根中镁、钙、锰、铁、锌、铜和硼等元素的吸收转运体基因表达上调。说明植物根和叶均可吸收利用CIF, CIF亦具有作为植物生长调节剂的应用潜力。
Synthetic small signaling peptide CIF was used to treat Arabidopsis. The effects of CIFs on the absorption and accumulation of mineral elements in roots and leaves were studied. The results were as follows:Root treatment with 0.1 μmol·L~(-1) CIF could significantly promote the accumulation of mineral elements such as Mg, Ca, Mn, Fe, Zn, Cu and B in Arabidopsis roots. Leaf treatment with CIF could significantly increase the contents of Mg, Ca, Mn, Fe, Zn and Cu in Arabidopsis roots. CIF had little effect on the microelement content in leaves. CIF could significantly induce transporter gene expression of Mg, Ca, Mn, Fe, Zn, Cu and B in roots.It indicated that CIF could be absorbed and utilized by plant roots and leaves, and CIF could also be used as growth regulator.
Synthetic small signaling peptide CIF was used to treat Arabidopsis. The effects of CIFs on the absorption and accumulation of mineral elements in roots and leaves were studied. The results were as follows:Root treatment with 0.1 μmol·L~(-1) CIF could significantly promote the accumulation of mineral elements such as Mg, Ca, Mn, Fe, Zn, Cu and B in Arabidopsis roots. Leaf treatment with CIF could significantly increase the contents of Mg, Ca, Mn, Fe, Zn and Cu in Arabidopsis roots. CIF had little effect on the microelement content in leaves. CIF could significantly induce transporter gene expression of Mg, Ca, Mn, Fe, Zn, Cu and B in roots.It indicated that CIF could be absorbed and utilized by plant roots and leaves, and CIF could also be used as growth regulator.
引文
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Mohd-Radzman NA,Laffont C,Ivanovici A,et al(2016).Different pathways act downstream of the peptide receptor CRA2 to regulate lateral root and nodule development.Plant Physiol,171(4):2536-2548
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Olsson V,Joos L,Zhu S,et al(2019).Look closely,the beautiful may be small:precursor-derived peptides in plants.Annu Rev Plant Biol,70:153-186
Pearce G,Strydom D,Johnson S,et al(1991).A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins.Science,253(5022):895-897
Rodriguez-Villalon A,Gujas B,Van Wijk R,et al(2015).Primary root protophloem differentiation requires balanced phosphatidylinositol-4,5-biphosphate levels and systemically affects root branching.Development,142(8):1437-1446
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Taiz L,Zeiger E(2006).Plant Physiology.4th ed.Oxford University Press,47-50
Takahashi F,Suzuki T,Osakabe Y,et al(2018).A small peptide modulates stomatal control via abscisic acid in long-distance signalling.Nature,556(7700):235-238
Takano J,Wada M,Ludewig U,et al(2006).The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation.Plant Cell,18(6):1498-509
Tanaka N,Fujiwara T,Tomioka R,et al(2015).Characterization of the histidine-rich loop of Arabidopsis vacuolar membrane zinc transporter AtMTP1 as a sensor of zinc level in the cytosol.Plant Cell Physiol,56(3):510-519
Tomatsu H,Takano J,Takahashi H,et al(2007).An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil.Proc Natl Acad Sci USA,104(47):18807-18812
Wang J,Li H,Han Z,et al(2015).Allosteric receptor activation by the plant peptide hormone phytosulfokine.Nature,525(7568):265-268
Wang T,Liang L,Xue Y,et al(2016).A receptor heteromer mediates the male perception of female attractants in plants.Nature,531(7593):241-244
Wang YY,Cheng Y,Chen KE,et al(2018).Nitrate transport,signaling,and use efficiency.Ann Rev Plant Biol,69:85-122
Zhou Y,Liu X,Engstrom EM,et al(2015).Control of plant stem cell function by conserved interacting transcriptional regulators.Nature,517(7534):377-380
Cheng NH,Pittman JK,Shigaki T,et al(2002).Characterization of CAX4,an Arabidopsis H+/cation antiporter.Plant Physiol,128(4):1245-1254
Eide D,Broderius M,Fett J,et al(1996).A novel iron-regulated metal transporter from plants identified by functional expression in yeast.Proc Natl Acad Sci USA,93(11):5624-5628
Foster KJ,Miklavcic SJ.(2017).A Comprehensive biophysical model of ion and water transport in plant roots.I.Clarifying the roles of endodermal barriers in the salt stress response.Front Plant Sci,8:1326
Gebert M,Meschenmoser K,SvidováS,et al(2009).ARoot-expressed magnesium transporter of the MRS2/MGTgene family in Arabidopsis thaliana allows for growth in low-Mg2+environments.Plant Cell,21(12):4018-4030
Hewitt EJ(2003).The role of the mineral elements in plant nutrition.Annu Rev Plant Physiol,2(2):25-52
Huffaker A,Pearce G,Ryan CA(2006).An endogenous peptide signal in Arabidopsis activates components of the innate immune response.Proc Natl Acad Sci USA,103(26):10098-10103
Ihnatowicz A,Siwinska J,Meharg AA,et al(2014).Conserved histidine of metal transporter AtNRAMP1 is crucial for optimal plant growth under manganese deficiency at chilling temperatures.New Phytol,202(4):11731183
Lease KA,Walker JC(2006).The Arabidopsis unannotated secreted peptide database,a resource for plant peptidomics.Plant Physiol,142(3):831-838
Matsubayashi Y(2014).Posttranslationally modified small-peptide signals in plants.Annu Rev Plant Biol,65(1):385-413
Matsuzaki Y,Ogawa-Ohnishi M,Mori A,et al(2010).Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis.Science,329(5995):1065-1067
Mohd-Radzman NA,Laffont C,Ivanovici A,et al(2016).Different pathways act downstream of the peptide receptor CRA2 to regulate lateral root and nodule development.Plant Physiol,171(4):2536-2548
Murphy E,Smith S,De Smet I(2012).Small signaling peptides in Arabidopsis development:how cells communicate over a short distance.Plant Cell,24(8):3198-3217
Nakaminami K,Okamoto M,Higuchi-Takeuchi M,et al(2018).AtPep3 is a hormone-like peptide that plays a role in the salinity stress tolerance of plants.Proc Natl Acad Sci USA,115(22):5810-5815
Nakayama T,Shinohara H,Tanaka M,et al(2017).A peptide hormone required for casparian strip diffusion barrier formation in Arabidopsis roots.Science,355(6322):284-286
Oh E,Seo PJ,Kim J(2018).Signaling peptides and receptors coordinating plant root development.Trends Plant Sci,23(4):337-351
Okamoto S,Shinohara H,mori T,et al(2012).Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase.Nat Commun,4(4):2191
Olsson V,Joos L,Zhu S,et al(2019).Look closely,the beautiful may be small:precursor-derived peptides in plants.Annu Rev Plant Biol,70:153-186
Pearce G,Strydom D,Johnson S,et al(1991).A polypeptide from tomato leaves induces wound-inducible proteinase inhibitor proteins.Science,253(5022):895-897
Rodriguez-Villalon A,Gujas B,Van Wijk R,et al(2015).Primary root protophloem differentiation requires balanced phosphatidylinositol-4,5-biphosphate levels and systemically affects root branching.Development,142(8):1437-1446
Suk LJ,Marketa H,Michal M,et al(2015).Competitive binding of antagonistic peptides fine-tunes stomatal patterning.Nature,522(7557):439-443
Sun TT,Zhang LL,Wang Q,et al(2015).Rice peptidomics based on nano LC-MS/MS analysis.Plant Physiol J,51(7):1173-117(in Chinese with English abstract)[孙婷婷,张乐乐,王倩等(2015).基于Nano LC-MS/MS的水稻多肽组学研究.植物生理学报,51(7):1173-1178]
Taiz L,Zeiger E(2006).Plant Physiology.4th ed.Oxford University Press,47-50
Takahashi F,Suzuki T,Osakabe Y,et al(2018).A small peptide modulates stomatal control via abscisic acid in long-distance signalling.Nature,556(7700):235-238
Takano J,Wada M,Ludewig U,et al(2006).The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation.Plant Cell,18(6):1498-509
Tanaka N,Fujiwara T,Tomioka R,et al(2015).Characterization of the histidine-rich loop of Arabidopsis vacuolar membrane zinc transporter AtMTP1 as a sensor of zinc level in the cytosol.Plant Cell Physiol,56(3):510-519
Tomatsu H,Takano J,Takahashi H,et al(2007).An Arabidopsis thaliana high-affinity molybdate transporter required for efficient uptake of molybdate from soil.Proc Natl Acad Sci USA,104(47):18807-18812
Wang J,Li H,Han Z,et al(2015).Allosteric receptor activation by the plant peptide hormone phytosulfokine.Nature,525(7568):265-268
Wang T,Liang L,Xue Y,et al(2016).A receptor heteromer mediates the male perception of female attractants in plants.Nature,531(7593):241-244
Wang YY,Cheng Y,Chen KE,et al(2018).Nitrate transport,signaling,and use efficiency.Ann Rev Plant Biol,69:85-122
Zhou Y,Liu X,Engstrom EM,et al(2015).Control of plant stem cell function by conserved interacting transcriptional regulators.Nature,517(7534):377-380