不同贮藏年限宣木瓜中四种有机酸含量测定比较
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摘要
目的:通过测定宣木瓜齐墩果酸、熊果酸以及绿原酸、原儿茶酸的含量,为不同贮藏年限的宣木瓜药材质量评价提供依据。方法:选择常规贮藏1年、2年、5年、7年采收的宣木瓜,采用高效液相色谱法进行含量测定。结果:齐墩果酸、熊果酸、绿原酸和原儿茶酸分别在0.02～20、0.02～20、0.02～20、0.01～24μg范围内线性关系良好。平均回收率分别为100.38%、102.45%、100.26%、101.23%。RSD分别为1.85%、1.78%、0.29%、1.62%。结论:贮藏1年、2年、5年、7年的宣木瓜中四种有机酸成分含量无明显差异。
Objective:To provide basis for quality control and evaluation of Chaenomeles speciosa(Sweet)Nakai for different storage years by measuring the content of oleanolic acid,ursolic acid,and chlorogenic acid and protocatechuic acid in Chaenomeles speciosa(Sweet)Nakai. Methods:The content was determined by high performance liquid chromatography. Results:Oleanolic acid,ursolic acid,chlorogenic acid and protocatechuic acid showed good linearity in the range of 0.02-20 μg,0.02-20 μg,0.02-20 μg,and 0.01-24 μg,respectively. The average recoveries were 100.38%,102.45%,100.26%and 101.23%,respectively. RSD was 1.85%,1.78%,0.29%,1.62%. Conclusion:This method is simple and feasible. It is accurate for the determination of four components in Chaenomeles speciosa(Sweet)Nakai with different storage ages,and it is suitable for qualitative and quantitative analysis.
Objective:To provide basis for quality control and evaluation of Chaenomeles speciosa(Sweet)Nakai for different storage years by measuring the content of oleanolic acid,ursolic acid,and chlorogenic acid and protocatechuic acid in Chaenomeles speciosa(Sweet)Nakai. Methods:The content was determined by high performance liquid chromatography. Results:Oleanolic acid,ursolic acid,chlorogenic acid and protocatechuic acid showed good linearity in the range of 0.02-20 μg,0.02-20 μg,0.02-20 μg,and 0.01-24 μg,respectively. The average recoveries were 100.38%,102.45%,100.26%and 101.23%,respectively. RSD was 1.85%,1.78%,0.29%,1.62%. Conclusion:This method is simple and feasible. It is accurate for the determination of four components in Chaenomeles speciosa(Sweet)Nakai with different storage ages,and it is suitable for qualitative and quantitative analysis.
引文
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[14]丁小娟,丁珊珊,吕凌,等. HPLC法同时测定宣木瓜中五种有机酸和原儿茶酸的含量[J].安徽医药,2013,17(9):1496-1498.
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[16]王晓阁,龙全江,周宙,等.不同产地加工法对宣木瓜药材中齐墩果酸和熊果酸含量的影响[J].中国中医药信息杂志,2014,21(11):75-78.
[17]王岱杰,王晓耿,岩玲,等.反相高效液相色谱测定不同品种皱皮木瓜中齐墩果酸和熊果酸含量[J].食品科学,2008,29(10):497-499.
[18]林玲,张琴,赖森亚,等. HPLC法测定菊花中绿原酸含量的不确定度分析[J].辽宁中医药大学学报,2014,16(1):68-69.
[19]张萌,郑杨,李盈. HPLC法测定东北铁线莲中齐墩果酸含量[J].辽宁中医药大学学报,2009,11(1):163-164.
[20]程菁菁.木瓜采收期和贮藏条件的研究[D].合肥:安徽中医药大学,2012.
[2]彭华胜,王德群,彭代银.道地药材“皖药”的形成及其界定[J].中国中药杂志,2017,42(9):1617-1622.
[3]戴敏,魏伟,汪倪萍,等.木瓜苷对大鼠佐剂性关节炎的治疗作用[J].中国药理学通报,2003,19(3):340-344.
[4]戴敏,魏伟.木瓜苷对免疫性关节炎大鼠滑膜细胞超微结构及分泌功能的影响[J].中国中医药科技,2003,10(3):154-155.
[5]张玲玲,魏伟,严尚学,等.木瓜苷对小鼠胶原性关节炎的预防作用及初步机制[J].中国药理学通报,2004,20(1):95-100.
[6]Dai Min,Wei Wei,Shen-yu Xian Zheng,et al. Glucosides ofChaenomeles speciosa remit rat adjuvant arthritis by inhibitingsynoviocyte activities[J]. Acta Pharmacol Sin,2003,24(11):1161-1166.
[7]王海燕,覃慧林,张永峰,等.木瓜三萜对佐剂性关节炎大鼠滑膜组织中Akt,NF-κB和促炎因子的表达影响[J].中国实验方剂学杂志,2017,25(5):141-146.
[8]覃慧林,张永峰,王心怡,等.木瓜乙酸乙酯萃取部位对急性胃溃疡小鼠胃黏膜中miR-423-5p、TFF1和p53表达的影响[J].中药药理与临床,2016,32(3):80-82.
[9]孔劲松,杨兴海,柳蔚.皱皮木瓜总黄酮对胃肠平滑肌的松弛作用及其机制分析[J].时珍国医国药,2007,18(9):2123-2124.
[10]张玲,谢晓梅,彭华胜,等.不同产地木瓜药材中齐墩果酸和熊果酸的比较研究[J].中药材,2009,32(5):673-676.
[11]刘慧妍,黄馨慧,张艳海. HPLC法快速测定8种中药材中齐墩果酸和熊果酸[J].中草药,2017,48(10):1998-2001.
[12]陶君彦,张晓昱,黄志军,等. HPLC法同时测定木瓜中绿原酸、咖啡酸的含量[J].中国药房,2007,18(12):921-924.
[13]丁姗姗,吕凌,张毅,等. HPLC法同时测定宣木瓜中绿原酸、芦丁、金丝桃苷、槲皮素的含量[J].安徽医药,2012,16(4):450-452.
[14]丁小娟,丁珊珊,吕凌,等. HPLC法同时测定宣木瓜中五种有机酸和原儿茶酸的含量[J].安徽医药,2013,17(9):1496-1498.
[15]李霞,杨颖博,李君丽,等. HPLC法同时测定皱皮木瓜中原儿茶酸和绿原酸的含量[J].中国医药导报,2012,9(34):110-112.
[16]王晓阁,龙全江,周宙,等.不同产地加工法对宣木瓜药材中齐墩果酸和熊果酸含量的影响[J].中国中医药信息杂志,2014,21(11):75-78.
[17]王岱杰,王晓耿,岩玲,等.反相高效液相色谱测定不同品种皱皮木瓜中齐墩果酸和熊果酸含量[J].食品科学,2008,29(10):497-499.
[18]林玲,张琴,赖森亚,等. HPLC法测定菊花中绿原酸含量的不确定度分析[J].辽宁中医药大学学报,2014,16(1):68-69.
[19]张萌,郑杨,李盈. HPLC法测定东北铁线莲中齐墩果酸含量[J].辽宁中医药大学学报,2009,11(1):163-164.
[20]程菁菁.木瓜采收期和贮藏条件的研究[D].合肥:安徽中医药大学,2012.