竹根七药材质量标准研究
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摘要
目的:对不同产地竹根七样品进行分析研究,建立竹根七药材质量标准。方法:鉴别竹根七的植物形态、药材性状、显微特征。采用TLC法进行定性鉴别。采用HPLC法(乙腈A-水B体系:0～15min,15%～23%A;15～33min,23%～38%A。33～40min,38%～100%A;40～45min,100%～100%A)测定测竹根七中Tupistroside E含量。建立竹根七的指纹图谱方法。结果:对竹根七的植物形态、药材性状、显微特征进行了描述。建立了竹根七的TLC鉴别方法,斑点清晰,分离度好,具有较好的专属性;根据3批次竹根七含量测定结果,Tupistroside E在2.85～23.00μg与峰面积呈现良好的线性关系,平均回收率均大于97%,RSD均小于2%(n=3)。不同产地竹根七(红河谷,汉中和凤县)中Tupistroside E的含量分别为3.078%,3.010%和3.724%(n=3),其中陕西凤县最高,红河谷其次,汉中最低。并建立了竹根七的指纹图谱,确立了15个共有峰。结论:所建立的方法快速、准确、重现性好,可作为竹根七药材质量标准评价的依据。
Objective:To establish the quality control standard for Tupistra chinensis Baker.from three producing areas and batches.Methods:Plant morphology,microscopic characteristics and medicinal properties of Tupistra chinensis were identified.TLC was used for qualitative identification.HPLC[Water(B)and acetonitrile(A):0～15 min,15%～23% A;15～33 min,23%～38% A.33～40 min,38%～100% A;40～45 min,100%～100% A]were used to determine the content of Tupistroside E from Tupistra chinensis Baker.Results:The plant morphology,medicinal characters and microscopic characteristics of Tupistra chinensis were described.TLC was established to identify Tupistra chinensis,the spots were clear and well separated,with good specificity.According to the determination results,when the content of 3 batches of Tupistroside E in Tupistra chinensis was at 2.85～23.00μg,concentration and peak area showed a good linear relationship.The average recovery rate was more than 97%,RSD less than 2%(n=3).The content of Tupistroside E in three producing areas were 3.078 %,3.010 % and 3.724 %(n=3),respectively.The fingerprint of Tupistra chinensis was established,and 15 common peaks were established.Conclusion:The established method is fast,accurate and reproducible,and can be used for the quality evaluation of Tupistra chinensis Baker.
Objective:To establish the quality control standard for Tupistra chinensis Baker.from three producing areas and batches.Methods:Plant morphology,microscopic characteristics and medicinal properties of Tupistra chinensis were identified.TLC was used for qualitative identification.HPLC[Water(B)and acetonitrile(A):0～15 min,15%～23% A;15～33 min,23%～38% A.33～40 min,38%～100% A;40～45 min,100%～100% A]were used to determine the content of Tupistroside E from Tupistra chinensis Baker.Results:The plant morphology,medicinal characters and microscopic characteristics of Tupistra chinensis were described.TLC was established to identify Tupistra chinensis,the spots were clear and well separated,with good specificity.According to the determination results,when the content of 3 batches of Tupistroside E in Tupistra chinensis was at 2.85～23.00μg,concentration and peak area showed a good linear relationship.The average recovery rate was more than 97%,RSD less than 2%(n=3).The content of Tupistroside E in three producing areas were 3.078 %,3.010 % and 3.724 %(n=3),respectively.The fingerprint of Tupistra chinensis was established,and 15 common peaks were established.Conclusion:The established method is fast,accurate and reproducible,and can be used for the quality evaluation of Tupistra chinensis Baker.
引文
[1]宋小妹,刘海静,王薇,等.太白七药研究与应用[M].北京:人民卫生出版社,2010.
[2]李霞,吴芳,刘佩,等.竹根七HPLC-ELSD指纹图谱的研究及薯蓣皂苷的含量测定[J].中南药学,2017,15(11):1591-1594.
[3]杨秋琛,王慧娜,赵桦.开口箭药材生药学特征研究[J].陕西理工学院学报:自然科学版,2016,32(1):76-81.
[4]陈征,周天华.开口箭种质资源及其混伪品的SSR指纹图谱研究[J].陕西理工学院学报:自然科学版,2017,33(2):74-79.
[5]王小童,陈征,周天华.开口箭种质资源的SSR遗传多样性分析[J].陕西理工学院学报:自然科学版,2016,32(1):70-75,88
[6]李玉泽,李真,张东东,等.响应面法优选竹根七总皂苷提取工艺[J].中南药学,2015,30(8):811-814.
[7]寇智斌,夏晓慧,陆遥,等.竹根七中白桦脂醇的分离与含量测定[J].食品研究与开发,2015,36(23):125-127.
[8]段文琦,王瑞,晋楠,等.开口箭属植物药理作用的研究[J].农村经济与科技,2016,27(8):44-45.
[9]唐超智,杨献光,张玉玲,等.开口箭生理活性物质及其药理作用研究进展[J].河南师范大学学报:自然科学版,2016,44(2):114-118.
[10]邬昊洋,周园,李诺飞,等.开口箭醇提物抗慢性咽炎活性研究[J].三峡大学学报:自然科学版,2012,34(2):83-85.
[11]谢锦艳,许苗苗,刘银环,等.开口箭乙醇提取物诱导肝癌细胞HepG2凋亡活性研究[J].中南药学,2014,32(8):735-739.
[12]谢锦艳,张东东,李玉泽,等.开口箭皂苷诱导肿瘤细胞凋亡研究[J].中药药理与临床,2014(3):82-86.
[13]刘佳.竹根七抑制血管生成活性化合物的分离鉴定及体内作用过程分析[D].西安:西安交通大学,2007.
[14]佘定平.竹根七总皂苷及成分的制备和药理活性研究[D].西安:西安交通大学,2007.
[15]林仙明.珍贵药材竹节参规范化栽培技术研究[D].武汉:华中农业大学,2006.
[16]吕海亮.竹根七属(Disporopsis)系统学研究[D].北京:北京大学,1999.
[17]林奇泗,曹艳,高艺恬,等.火焰原子吸收光谱法测定深裂竹根七中的4种金属元素[J].光谱实验室,2012,21(6):3609-3613.
[18]张珏,覃容贵,安东琴,等.紫外可见分光光度法测定深裂竹根七多糖的含量[J].贵阳医学院学报,2014,24(3):369-371.
[19]郝俊杰,杨发建,王洪云.中药玉竹一种伪品深裂竹根七的鉴别研究[J].亚太传统医药,2015,17(22):20.
[2]李霞,吴芳,刘佩,等.竹根七HPLC-ELSD指纹图谱的研究及薯蓣皂苷的含量测定[J].中南药学,2017,15(11):1591-1594.
[3]杨秋琛,王慧娜,赵桦.开口箭药材生药学特征研究[J].陕西理工学院学报:自然科学版,2016,32(1):76-81.
[4]陈征,周天华.开口箭种质资源及其混伪品的SSR指纹图谱研究[J].陕西理工学院学报:自然科学版,2017,33(2):74-79.
[5]王小童,陈征,周天华.开口箭种质资源的SSR遗传多样性分析[J].陕西理工学院学报:自然科学版,2016,32(1):70-75,88
[6]李玉泽,李真,张东东,等.响应面法优选竹根七总皂苷提取工艺[J].中南药学,2015,30(8):811-814.
[7]寇智斌,夏晓慧,陆遥,等.竹根七中白桦脂醇的分离与含量测定[J].食品研究与开发,2015,36(23):125-127.
[8]段文琦,王瑞,晋楠,等.开口箭属植物药理作用的研究[J].农村经济与科技,2016,27(8):44-45.
[9]唐超智,杨献光,张玉玲,等.开口箭生理活性物质及其药理作用研究进展[J].河南师范大学学报:自然科学版,2016,44(2):114-118.
[10]邬昊洋,周园,李诺飞,等.开口箭醇提物抗慢性咽炎活性研究[J].三峡大学学报:自然科学版,2012,34(2):83-85.
[11]谢锦艳,许苗苗,刘银环,等.开口箭乙醇提取物诱导肝癌细胞HepG2凋亡活性研究[J].中南药学,2014,32(8):735-739.
[12]谢锦艳,张东东,李玉泽,等.开口箭皂苷诱导肿瘤细胞凋亡研究[J].中药药理与临床,2014(3):82-86.
[13]刘佳.竹根七抑制血管生成活性化合物的分离鉴定及体内作用过程分析[D].西安:西安交通大学,2007.
[14]佘定平.竹根七总皂苷及成分的制备和药理活性研究[D].西安:西安交通大学,2007.
[15]林仙明.珍贵药材竹节参规范化栽培技术研究[D].武汉:华中农业大学,2006.
[16]吕海亮.竹根七属(Disporopsis)系统学研究[D].北京:北京大学,1999.
[17]林奇泗,曹艳,高艺恬,等.火焰原子吸收光谱法测定深裂竹根七中的4种金属元素[J].光谱实验室,2012,21(6):3609-3613.
[18]张珏,覃容贵,安东琴,等.紫外可见分光光度法测定深裂竹根七多糖的含量[J].贵阳医学院学报,2014,24(3):369-371.
[19]郝俊杰,杨发建,王洪云.中药玉竹一种伪品深裂竹根七的鉴别研究[J].亚太传统医药,2015,17(22):20.