四环素对土壤碳氮矿化及烟草种子萌发的影响
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摘要
为明确抗生素残留的烟田生态效应,采用微宇宙试验系统研究四环素添加对土壤碳氮矿化及烟草种子萌发的影响。结果表明,中低量四环素添加(50、5 mg/kg)处理对土壤有机碳矿化速率无明显影响,但高添加量(500 mg/kg)处理的土壤有机碳累积矿化量在培养后期明显高于其他处理,在培养结束时比对照增加13.75%,显示对土壤有机碳矿化的一定刺激作用。四环素添加处理的土壤有机氮的矿化速率及硝化速率整体上均高于对照,特别是在培养前期(28 d)的土壤氮素硝化速率明显高于对照,后期则与对照差异不大。低浓度四环素溶液对烟草种子的发芽势及发芽率均无显著影响,但高浓度四环素溶液(>80 mg/L)则对烟草种子的发芽势和发芽率有显著抑制作用(P<0.05)。此外,四环素浓度与烟草种子根长的抑制率间具有良好的剂量-效应关系。其对根伸长抑制率的IC10、IC50分别为6.65、110.20 mg/L,而且根伸长抑制率与四环素浓度呈对数相关且相关性显著(P<0.05)。四环素长期暴露会在一定程度上影响烟田土壤的碳氮转化功能,并对烟草幼苗的生长发育具有不良影响,但四环素进入农田后对土壤养分转化及烟草生长发育的具体影响机制及其风险评价还需要进一步研究。
引文
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[23]王加龙,刘坚真,陈杖榴,等.恩诺沙星残留对土壤微生物数量及群落功能多样性的影响[J].应用与环境生物学报,2005,11(1):86-89.
[24]王金花,朱鲁生,王军,等.4种典型抗生素对土壤微生物呼吸的影响[J].农业环境科学学报,2011,30(11):2232-2236.
[25]Toth J D,Feng Y C,Dou Z X.Veterinary antibiotics at environmentally relevant concentrations inhibit soil iron reduction and nitrification[J].Soil Biology and Biochemistry,2011,43(12):2470-2472.
[26]Gao M L,Song W H,Zhou Q,et al.Interactive effect of oxytetracycline and lead on soil enzymatic activity and microbial biomass[J].Environmental Toxicology and Pharmacology,2013,36(2):667-674.
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[28]Gomez J,Mendez R,Lema J M.The effect of antibiotics on nitrification processes[J].Applied Biochemistry and Biotechnology,1996,57(1):869-876.
[29]王瑞,魏源送.畜禽粪便中残留四环素类抗生素和重金属的污染特征及其控制[J].农业环境科学学报,2013,32(9):1705-1719.
[30]葛成军,俞花美,焦鹏.两种四环素类兽药抗生素对白菜种子发芽与根伸长抑制的毒性效应[J].生态环境学报,2012,21(6):1143-1148.
[31]安婧,周启星,刘维涛.土霉素对小麦种子发芽与幼苗生长发育的生态毒性[J].环境科学,2009,30(10):3022-3027.
[32]Araùjo A F,Monteiro R R.Plant bioassays to assess toxicity of textile sludge compost[J].Scientia Agricola,2005,62(3):286-290.
[33]魏子艳,王金花,夏晓明,等.三种抗生素对蔬菜种子芽与根伸长的生态毒性效应[J].农业环境科学学报,2014,33(2):237-242.
[34]Migliore L,Godeas F,De Filippis S P,et al.Hormetic effect(s)of tetracyclines as environmental contaminant on Zea mays[J].Environmental Pollution,2010,158(1):129-134.
[35]Herklotz P A,Gurung P,Vanden H B,et al.Uptake of human pharmaceuticals by plants grown under hydroponic conditions[J].Chemosphere,2010,78(11):1416-1421.
[36]金彩霞,陈秋颖,刘军军,等.两种常用兽药对作物发芽的生态毒性效应[J].环境科学学报,2009,29(3):619-625.
[37]汤贝贝,张振华,卢信,等.养殖废水中抗生素的植物修复研究进展[J].江苏农业学报,2017,33(1):224-232.
[38]Chen Y,Zhou Q X.Ecological toxicity of reactive X-3B red dye and cadmium acting on wheat(Triticum aestivum)[J].Journal of Environmental Sciences,2002,14(1):136-140.
[39]Norman A G.Terramycin and plant growth 1[J].Agronomy Journal,1955,47(12):585-587.
[40]Figueroa R A,Mackay A A.Sorption of oxytetracycline to iron oxides and iron oxide-rich soils[J].Environmental Science and Technology,2005,39(17):6664-6671.
[41]Mackay A A,Canterbury B.Oxytetracycline sorption to organic matter by metal-bridging[J].Journal of Environmental Quality,2005,34(6):1964-1971.
[42]Pils J R,Laird D A.Sorption of tetracycline and chlortetracycline on K-and Ca-saturated soil clays,humic substances,and clayhumic complexes[J].Environmental Science and Technology,2007,41(6):1928-1933.
[43]陈博阳,余彬彬,钱晓晴,等.锌和土霉素胁迫对玉米种子发芽和幼苗抗氧化酶活性的影响[J].江苏农业学报,2017,33(1):13-18.
[44]张劲强,董元华,安琼,等.兽药抗生素在土壤环境中的行为[J].土壤,2005,37(4):353-361.
[2]张树清,张夫道,刘秀梅,等.规模化养殖畜禽粪主要有害成分测定分析研究[J].植物营养与肥料学报,2005,11(6):822-829.
[3]Sarmah A K,Meyer M T,Boxall A B.A global perspective on the use,sales,exposure pathways,occurrence,fate and effects of veterinary antibiotics(VAs)in the environment[J].Chemosphere,2006,65(5):725-759.
[4]Aust M O,Godlinski F,Travis G R,et al.Distribution of sulfamethazine,chlortetracycline and tylosin in manure and soil of Canadian feedlots after subtherapeutic use in cattle[J].Environmental Pollution,2008,156(3):1243-1251.
[5]Kools S A,Moltmann J F,Knacker T.Estimating the use of veterinary medicines in the European union[J].Regulatory Toxicology and Pharmacology,2008,50(1):59-65.
[6]Golet E M,Strehler A,Alder A C,et al.Determination of fluoroquinolone antibacterial agents in sewage sludge and sludgetreated soil using accelerated solvent extraction followed by solidphase extraction[J].Analytical Chemistry,2002,74(21):5455-5462.
[7]Warman P R,Thomas R L.Chlortetracycline in soil amended with poultry manure[J].Canadian Journal of Soil Science,1981,61(1):161-163.
[8]李伟明,鲍艳宇,周启星.四环素类抗生素降解途径及其主要降解产物研究进展[J].应用生态学报,2012,23(8):2300-2308.
[9]杨基峰,应光国,赖华杰,等.三种抗生素对土壤呼吸和硝化作用的影响[J].生态环境学报,2014,23(6):1050-1056.
[10]肖明月,安婧,纪占华,等.六种常见抗生素对小白菜种子萌发及生理特性的影响[J].生态学杂志,2014,33(10):2775-2781.
[11]鲍艳宇,周启星,谢秀杰.四环素类抗生素对小麦种子芽与根伸长的影响[J].中国环境科学,2008,28(6):566-570.
[12]文启孝.土壤有机质研究法[M].北京:农业出版社,1984.
[13]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.
[14]唐淦海,刘郡英,谷春豪,等.作物秸秆与城市污泥高温好氧堆肥产物对土壤氮矿化的影响[J].农业工程学报,2011,27(1):326-331.
[15]顾春朝,傅民杰,孙宇贺,等.不同施肥措施对稻田土壤氮素矿化的影响[J].广东农业科学,2015,42(5):43-48.
[16]周启星,孔繁翔,朱琳.生态毒理学[M].北京:科学出版社,2004.
[17]周启星,王美娥.土壤生态毒理学研究进展与展望[J].生态毒理学报,2006,1(1):1-11.
[18]Malchair S,Carnol M.Microbial biomass and C and N transformations in forest floors under European beech,sessile oak,Norway spruce and Douglas-fir at four temperate forest sites[J].Soil Biology and Biochemistry,2009,41(4):831-839.
[19]Cao J,Wang C,Dou Z H,et al.Independent and combined effects of oxytetracycline and ntibiotic-resistant Escherichia coli O157:H7 on soil microbial activity and partial nitrification processes[J].Soil Biology&Biocmistrhey,2016,98:138-147.
[20]Allison S D,Martiny J B.Resistance,resilience,and redundancy in microbial communities[J].Proceedings of the National Academy of Sciences of the USA,2008,105(S1):11512-11519.
[21]金彩霞,刘军军,陈秋颖,等.兽药磺胺间甲氧嘧啶对土壤呼吸及酶活性的影响[J].农业环境科学学报,2010,29(2):314-318.
[22]刘锋,应光国,周启星,等.抗生素类药物对土壤微生物呼吸的影响[J].环境科学,2009,30(5):1280-1285.
[23]王加龙,刘坚真,陈杖榴,等.恩诺沙星残留对土壤微生物数量及群落功能多样性的影响[J].应用与环境生物学报,2005,11(1):86-89.
[24]王金花,朱鲁生,王军,等.4种典型抗生素对土壤微生物呼吸的影响[J].农业环境科学学报,2011,30(11):2232-2236.
[25]Toth J D,Feng Y C,Dou Z X.Veterinary antibiotics at environmentally relevant concentrations inhibit soil iron reduction and nitrification[J].Soil Biology and Biochemistry,2011,43(12):2470-2472.
[26]Gao M L,Song W H,Zhou Q,et al.Interactive effect of oxytetracycline and lead on soil enzymatic activity and microbial biomass[J].Environmental Toxicology and Pharmacology,2013,36(2):667-674.
[27]Piotrowska-Seget Z,Engel R,Nowak E,et al.Successive soil treatment with captan or oxytetracycline affects non-target microorganisms[J].World Journal of Microbiology and Biotechnology,2008,24(12):2843-2848.
[28]Gomez J,Mendez R,Lema J M.The effect of antibiotics on nitrification processes[J].Applied Biochemistry and Biotechnology,1996,57(1):869-876.
[29]王瑞,魏源送.畜禽粪便中残留四环素类抗生素和重金属的污染特征及其控制[J].农业环境科学学报,2013,32(9):1705-1719.
[30]葛成军,俞花美,焦鹏.两种四环素类兽药抗生素对白菜种子发芽与根伸长抑制的毒性效应[J].生态环境学报,2012,21(6):1143-1148.
[31]安婧,周启星,刘维涛.土霉素对小麦种子发芽与幼苗生长发育的生态毒性[J].环境科学,2009,30(10):3022-3027.
[32]Araùjo A F,Monteiro R R.Plant bioassays to assess toxicity of textile sludge compost[J].Scientia Agricola,2005,62(3):286-290.
[33]魏子艳,王金花,夏晓明,等.三种抗生素对蔬菜种子芽与根伸长的生态毒性效应[J].农业环境科学学报,2014,33(2):237-242.
[34]Migliore L,Godeas F,De Filippis S P,et al.Hormetic effect(s)of tetracyclines as environmental contaminant on Zea mays[J].Environmental Pollution,2010,158(1):129-134.
[35]Herklotz P A,Gurung P,Vanden H B,et al.Uptake of human pharmaceuticals by plants grown under hydroponic conditions[J].Chemosphere,2010,78(11):1416-1421.
[36]金彩霞,陈秋颖,刘军军,等.两种常用兽药对作物发芽的生态毒性效应[J].环境科学学报,2009,29(3):619-625.
[37]汤贝贝,张振华,卢信,等.养殖废水中抗生素的植物修复研究进展[J].江苏农业学报,2017,33(1):224-232.
[38]Chen Y,Zhou Q X.Ecological toxicity of reactive X-3B red dye and cadmium acting on wheat(Triticum aestivum)[J].Journal of Environmental Sciences,2002,14(1):136-140.
[39]Norman A G.Terramycin and plant growth 1[J].Agronomy Journal,1955,47(12):585-587.
[40]Figueroa R A,Mackay A A.Sorption of oxytetracycline to iron oxides and iron oxide-rich soils[J].Environmental Science and Technology,2005,39(17):6664-6671.
[41]Mackay A A,Canterbury B.Oxytetracycline sorption to organic matter by metal-bridging[J].Journal of Environmental Quality,2005,34(6):1964-1971.
[42]Pils J R,Laird D A.Sorption of tetracycline and chlortetracycline on K-and Ca-saturated soil clays,humic substances,and clayhumic complexes[J].Environmental Science and Technology,2007,41(6):1928-1933.
[43]陈博阳,余彬彬,钱晓晴,等.锌和土霉素胁迫对玉米种子发芽和幼苗抗氧化酶活性的影响[J].江苏农业学报,2017,33(1):13-18.
[44]张劲强,董元华,安琼,等.兽药抗生素在土壤环境中的行为[J].土壤,2005,37(4):353-361.