骆驼刺提取物对腹泻型肠易激综合征模型大鼠水液代谢和胃肠激素水平的影响
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摘要
目的探讨骆驼刺提取物对腹泻型肠易激综合征(D-IBS)大鼠水液代谢和胃肠激素水平的影响。方法将50只SD大鼠随机分成5组,每组各10只。除正常组(给予生理盐水灌胃)外,模型组(生理盐水)、骆驼刺提取物高剂量组(400 mg·kg~(-1))、低剂量组(200 mg·kg~(-1))和阳性对照组(曲美布汀60 mg·kg~(-1))采用传统束缚应激刺激联合高乳糖饮食建立D-IBS大鼠模型后再给予相应药物灌胃干预,每日给药1次。用药7 d后,计算粪便含水率;用药10 d后,进行腹部收缩反射(AWR)评分,并统计24 h内粪便粒数。采用酶联免疫吸附(ELISA)法测定各组大鼠血浆中胃肠激素血管活性肠肽(VIP)、 5-羟色胺(5-HT)、 P物质(SP)和胃动素(MTL)的水平。结果与正常组比较,模型组粪便含水率、 AWR评分、 VIP、 5-HT、 SP和MTL水平均显著增加(P <0.01),大鼠24 h内粪便粒数明显减少(P <0.01)。与模型组相比,骆驼刺提取物高剂量组和低剂量组粪便含水率与AWR评分, 5-HT、 SP、 MTL水平均降低(P <0.05),大鼠24 h内粪便粒数明显增加(P <0.01)。其中,骆驼刺提取物低剂量组VIP水平与模型组相比降低(P <0.05),骆驼刺提取物高剂量组的VIP水平与模型组相比无显著差异(P> 0.05)。结论骆驼刺提取物可改善D-IBS大鼠肠道高敏感性和腹泻,其作用可能与降低VIP、 5-HT、 SP、 MTL的水平有关。
AIM To investigate the effects of the extract of Alhagi sparsifolia Shap on the metabolism of water and gastrointestinal hormones in rats with diarrhea-predominant of irritable bowel syndrome( D-IBS).METHODS Fifty Sprague-Dawley rats were randomly divided into five groups(10 in each). In addition to the normal group(saline), each group was treated with traditional restraint stress stimulation combined with high lactose diet to establish a D-IBS rat model. And then, model group(saline), Alhagi sparsifolia Shap extract high dose group( 400 mg·kg~(-1)), low dose group(200 mg·kg~(-1)) and positive control group( trimebutine 60 mg·kg~(-1)) was given corresponding drugs by gavage once a day. Seven days later, the fecal moisture content was calculated. Ten days later, the abdominal contraction reflex(AWR) score was evaluated, and the number of feces was counted within 24 hours. The levels of gastrointestinal hormones like vasoactive intestinal peptide(VIP), 5-hydroxytryptamine(5-HT), substance P(SP) and motilin(MTL) in plasma of rats in each group were determined by enzyme-linked immunosorbent assay( ELISA). RESULTS Compared with the normal group, the fecal moisture content, AWR score and VIP, 5-HT, SP, MTL level in the model group were significantly increased( P < 0.01) in the model group, and the number of fecal granules was significantly decreased within 24 hours(P < 0.01). Compared with the model group, the fecal moisture content, the AWR score and the level of 5-HT, SP, MTL in the Alhagi sparsifolia Shap high and low dose groups were significantly decreased(P < 0.05), and the number of fecal grains increased significantly within 24 hours(P <0.01). The level of VIP in the Alhagi sparsifolia Shap low dose group was significantly lower than that in the model group(P < 0.05). The level of VIP in the Alhagi sparsifolia Shap high dose group was not significantly different with that in the model group(P > 0.05). CONCLUSION The extract of Alhagi sparsifolia Shap can improve the high sensitivity and diarrhea of intestinal tract of D-IBS rats, which may be related to the decrease of the levels of VIP, 5-HT, SP and MTL.
AIM To investigate the effects of the extract of Alhagi sparsifolia Shap on the metabolism of water and gastrointestinal hormones in rats with diarrhea-predominant of irritable bowel syndrome( D-IBS).METHODS Fifty Sprague-Dawley rats were randomly divided into five groups(10 in each). In addition to the normal group(saline), each group was treated with traditional restraint stress stimulation combined with high lactose diet to establish a D-IBS rat model. And then, model group(saline), Alhagi sparsifolia Shap extract high dose group( 400 mg·kg~(-1)), low dose group(200 mg·kg~(-1)) and positive control group( trimebutine 60 mg·kg~(-1)) was given corresponding drugs by gavage once a day. Seven days later, the fecal moisture content was calculated. Ten days later, the abdominal contraction reflex(AWR) score was evaluated, and the number of feces was counted within 24 hours. The levels of gastrointestinal hormones like vasoactive intestinal peptide(VIP), 5-hydroxytryptamine(5-HT), substance P(SP) and motilin(MTL) in plasma of rats in each group were determined by enzyme-linked immunosorbent assay( ELISA). RESULTS Compared with the normal group, the fecal moisture content, AWR score and VIP, 5-HT, SP, MTL level in the model group were significantly increased( P < 0.01) in the model group, and the number of fecal granules was significantly decreased within 24 hours(P < 0.01). Compared with the model group, the fecal moisture content, the AWR score and the level of 5-HT, SP, MTL in the Alhagi sparsifolia Shap high and low dose groups were significantly decreased(P < 0.05), and the number of fecal grains increased significantly within 24 hours(P <0.01). The level of VIP in the Alhagi sparsifolia Shap low dose group was significantly lower than that in the model group(P < 0.05). The level of VIP in the Alhagi sparsifolia Shap high dose group was not significantly different with that in the model group(P > 0.05). CONCLUSION The extract of Alhagi sparsifolia Shap can improve the high sensitivity and diarrhea of intestinal tract of D-IBS rats, which may be related to the decrease of the levels of VIP, 5-HT, SP and MTL.
引文
[1]李海龙,任维,李彦敏,等.肠易激综合征相关致病因素及发病机制研究进展[J].医学与哲学(B), 2017, 38(2):73-76.
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[4]邓桂球,张蓓,张喆,等.六味顺激胶囊对肝郁脾虚型肠易激综合征模型大鼠内脏敏感性及五羟色胺水平的影响[J].中国中医药信息杂志, 2014, 21(8):46-48.
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[8]马晓玲,夏提古丽·阿不利孜,石磊岭,等.慢性束缚应激联合特殊饮食制备腹泻性肠易激综合征(IBS-D)大鼠模型的研究[J].中国比较医学杂志, 2018, 28(7):12-17.
[9]李依洁,苏晓兰,杨晨,等.脾肾阳虚型肠易激综合征大鼠模型的建立与评价[J].中国中西医结合杂志, 2017, 37(8):950-954.
[10]石卿,赵云,梅竹松,等. 5-羟色胺与褪黑素在母婴分离致腹泻型肠易激综合征模型大鼠中的表达[J].军事医学,2013, 37(8):604-608.
[11]陆敏,黄厚才,钟荣玲,等.肠康方对肠易激综合征内脏高敏感模型大鼠的作用[J].中国中西医结合消化杂志, 2012, 20(1):15-18.
[12]马薇,龙霖梓,彭芝配,等.九香止泻片对腹泻型肠易激综合征大鼠血浆VIP、NPY和肠黏膜5-HT的影响[J].湖南中医药大学学报, 2009, 29(6):29-32.
[13] CREMON C, CARINI G, WANG B, et al. Intestinal serotonin release, sensory neuron activation, and abdominal pain in irritable bowel syndrome[J]. Am J Gastroenterol, 2011, 106(7):1290-1298.
[14]陈晓敏,罗云,吴跃龙,等.肠易激综合征患者结肠黏膜辣椒素受体、P物质和肥大细胞变化的研究[J].胃肠病学, 2010,15(11):672-675.
[15]杨梅,赵瑞亭,许立,等.温胃调肠颗粒对腹泻型肠易激综合征大鼠的作用机制研究[J].南京中医药大学学报, 2016,32(4):362-366.
[16]谢宁,李曼,唐鄂.胆舒胶囊联合酪酸梭菌活菌片治疗肠易激综合征的临床疗效[J].中国新药与临床杂志, 2018, 37(11):651-654.
[17]周次利,吴璐一,吴蓓玲,等.艾灸及其生成物对腹泻型肠易激综合症模型大鼠内脏痛和结肠水液代谢的影响[J].世界科学技术-中医药现代化, 2014, 16(6):1261-1267.
[18]程晓雯,林中,郑清华,等.重症急性胰腺炎伴胃肠动力障碍大鼠结肠黏膜下血管活性肠肽神经元的变化[J].南京医科大学学报:自然科学版, 2012, 32(2):183-187.
[19]朱力阳,彭成,谢晓芳,等.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报, 2010, 26(2):267-270.
[20]俞媛洁,陈继红,罗和生,等. 5-羟色胺与结肠动力关系研究[J].胃肠病学和肝病学杂志, 2014, 23(1):99-103.
[21]李宁宁,方秀才.脑-肠轴在肠易激综合征发病中的作用[J].胃肠病学和肝病学杂志, 2013, 22(2):163-166.
[22]陈力.氟西汀联合双歧三联活菌胶囊治疗性肠易激综合征的临床观察[J].牡丹江医学院学报, 2010, 31(6):41-42.
[2]刘丽娜,孙志广,石伟,等.金荞麦提取物抑制腹泻型肠易激综合征大鼠的肠运动功能[J].中国临床研究, 2012, 25(12):1153-1155.
[3] WINSTON J, SHENOY M, MEDLEY D, et al. The vanilloid receptor initiates and maintains colonic hypersensitivity induced by neonatal colon irritation in rats[J]. Gastroenterology, 2007,132(2):615-627.
[4]邓桂球,张蓓,张喆,等.六味顺激胶囊对肝郁脾虚型肠易激综合征模型大鼠内脏敏感性及五羟色胺水平的影响[J].中国中医药信息杂志, 2014, 21(8):46-48.
[5]马晓玲,徐建国,夏提古丽·阿不利孜,等.骆驼刺提取物对免疫抑制小鼠迟发型超敏反应的作用[J].新疆医学, 2017, 47(10):1103-1105.
[6]马晓玲,石磊岭,刘雪松,等.骆驼刺提取物对腹泻型肠易激综合征(IBS-D)模型大鼠腹壁肌电活动及血清NO水平的调节作用研究[J].中药药理与临床, 2018, 34(5):78-80.
[7]魏鸿雁,马晓玲,夏提古丽·阿不利孜,等.骆驼刺提取物作为制备治疗肠易激综合征药物的应用[P].新疆:CN105943592A,2016-09-21.
[8]马晓玲,夏提古丽·阿不利孜,石磊岭,等.慢性束缚应激联合特殊饮食制备腹泻性肠易激综合征(IBS-D)大鼠模型的研究[J].中国比较医学杂志, 2018, 28(7):12-17.
[9]李依洁,苏晓兰,杨晨,等.脾肾阳虚型肠易激综合征大鼠模型的建立与评价[J].中国中西医结合杂志, 2017, 37(8):950-954.
[10]石卿,赵云,梅竹松,等. 5-羟色胺与褪黑素在母婴分离致腹泻型肠易激综合征模型大鼠中的表达[J].军事医学,2013, 37(8):604-608.
[11]陆敏,黄厚才,钟荣玲,等.肠康方对肠易激综合征内脏高敏感模型大鼠的作用[J].中国中西医结合消化杂志, 2012, 20(1):15-18.
[12]马薇,龙霖梓,彭芝配,等.九香止泻片对腹泻型肠易激综合征大鼠血浆VIP、NPY和肠黏膜5-HT的影响[J].湖南中医药大学学报, 2009, 29(6):29-32.
[13] CREMON C, CARINI G, WANG B, et al. Intestinal serotonin release, sensory neuron activation, and abdominal pain in irritable bowel syndrome[J]. Am J Gastroenterol, 2011, 106(7):1290-1298.
[14]陈晓敏,罗云,吴跃龙,等.肠易激综合征患者结肠黏膜辣椒素受体、P物质和肥大细胞变化的研究[J].胃肠病学, 2010,15(11):672-675.
[15]杨梅,赵瑞亭,许立,等.温胃调肠颗粒对腹泻型肠易激综合征大鼠的作用机制研究[J].南京中医药大学学报, 2016,32(4):362-366.
[16]谢宁,李曼,唐鄂.胆舒胶囊联合酪酸梭菌活菌片治疗肠易激综合征的临床疗效[J].中国新药与临床杂志, 2018, 37(11):651-654.
[17]周次利,吴璐一,吴蓓玲,等.艾灸及其生成物对腹泻型肠易激综合症模型大鼠内脏痛和结肠水液代谢的影响[J].世界科学技术-中医药现代化, 2014, 16(6):1261-1267.
[18]程晓雯,林中,郑清华,等.重症急性胰腺炎伴胃肠动力障碍大鼠结肠黏膜下血管活性肠肽神经元的变化[J].南京医科大学学报:自然科学版, 2012, 32(2):183-187.
[19]朱力阳,彭成,谢晓芳,等.化学刺激引起的两种大鼠内脏高敏感性模型的建立[J].中国药理学通报, 2010, 26(2):267-270.
[20]俞媛洁,陈继红,罗和生,等. 5-羟色胺与结肠动力关系研究[J].胃肠病学和肝病学杂志, 2014, 23(1):99-103.
[21]李宁宁,方秀才.脑-肠轴在肠易激综合征发病中的作用[J].胃肠病学和肝病学杂志, 2013, 22(2):163-166.
[22]陈力.氟西汀联合双歧三联活菌胶囊治疗性肠易激综合征的临床观察[J].牡丹江医学院学报, 2010, 31(6):41-42.