一株高产胞外多糖乳酸菌的分离鉴定及其产胞外多糖的研究
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摘要
该研究从自然发酵剁辣椒中分离筛选一株高产胞外多糖的乳酸菌,经形态观察、生理生化试验及分子生物学对其进行鉴定,并探索菌株产胞外多糖的最佳碳源和最佳培养时间。结果表明,筛选并鉴定得到一株高产胞外多糖的植物乳杆菌(Lactobacillus plantarum)Y-20。菌株Y-20产胞外多糖的最佳碳源为麦芽糖,且在MRS培养基中培养16 h时,OD_(600nm)值达到最大(3.62),胞外多糖产量达到最高,为242.95 mg/L。
In this study, a high-yield extracellular polysaccharide lactic acid bacterium was isolated and screened from natural fermented chopped pepper, and identified by morphological observation, physiological and biochemical tests and molecular biology. The optimal carbon source and culture time for the strain producing extracellular polysaccharide were explored. The results showed that Lactobacillus plantarum Y-20 with high-yield extracellular polysaccharide was screened and identified. The optimal carbon source for the strain Y-20 was maltose, and the OD_(600nm)value reached maximum(3.62) and the yield of extracellular polysaccharide reached the highest(242.95 mg/L) after cultured for 16 h in MRS medium.
In this study, a high-yield extracellular polysaccharide lactic acid bacterium was isolated and screened from natural fermented chopped pepper, and identified by morphological observation, physiological and biochemical tests and molecular biology. The optimal carbon source and culture time for the strain producing extracellular polysaccharide were explored. The results showed that Lactobacillus plantarum Y-20 with high-yield extracellular polysaccharide was screened and identified. The optimal carbon source for the strain Y-20 was maltose, and the OD_(600nm)value reached maximum(3.62) and the yield of extracellular polysaccharide reached the highest(242.95 mg/L) after cultured for 16 h in MRS medium.
引文
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[15]王兰,赵玲艳,陈思思,等.发酵辣椒中产亚硝酸盐还原酶短乳杆菌L5的选育及特性研究[J].中国酿造,2014,33(11):25-29.
[16]王昌禄,韩晓梅,陈勉华,等. Viili中胞外多糖的分离纯化及对秀丽线虫寿命的影响[J].氨基酸和生物资源,2011,33(2):64-67.
[17] SAIJA N, WELMAN A D, BENNETT R J. Development of a dairybased exopolysaccharide bioingredient[J]. Int Dairy, 2010, 20(9):603-608.
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[19]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:中国农业出版社,1995.
[20] MOZZI F, ROLLAN G, Q SAVOY G DE DIORI, et al. Effect of galactose and glucose on the exopolysaccharide production and the activities of biosynthetic enzymes in Lactobacillus casei CRL 87[J]. J Appl Microbiol,2001, 91:160-167.
[2]刘晶,杨森,陈杨扬,等.副干酪乳杆菌VL8产胞外多糖条件优化及其抗氧化性质[J].中国食品学报,2017,17(5):82-89.
[3]陶静,许赛信,孟德俊,等.乳酸菌发酵生产胞外多糖条件优化研究[J].食品工业,2017(1):28-31.
[4]苗君莅,于鹏,肖杨,等.胞外多糖的研究现状与展望[J].食品科技,2014,39(10):226-231.
[5] MIRONESCU M, MIRONESCU I D. Rheological behaviour of a novel microbial polysaccharide[J]. Romanian Biotechnol Lett, 2011, 16(2):6105-6114.
[6]万金敏,杨丽娜,葛武鹏,等.西藏牦牛奶渣中优势乳酸菌产胞外多糖及其耐受性[J].食品科学,2017,38(10):98-103.
[7] ZAJ譒EK K, GOR譒EK A, KOLAR M. Cultivating conditions effects on kefiran production by the mixed culture of lactic acid bacteria imbedded within kefir grains[J]. Food Chem, 2013, 139(1-4):970-977.
[8] BADEL S, BERNARDI T, MICHAUD P. New perspectives for Lactobacilli exopolysaccharides[J]. Biotechnol Adv, 2011, 29(1):54-66.
[9] CAGGIANIELLO G, KLEEREBEZEM M, SPANO G. Exopolysaccharides produced by lactic acid bacteria:from health-promoting benefits to stress tolerance mechanisms[J]. Appl Microbiol Biot, 2016, 100(9):3877-3886.
[10] TORINO M I, VALDEZ G F D, MOZZI F. Biopolymers from lactic acid bacteria. Novel applications in foods and beverages[J]. Front Microbiol, 2015, 6:834.
[11] CAO W, BENKUN Q, ZHAO J, et al. Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing beta-poly(malic acid)production by Aureobasidium pullulans ipe-1[J]. J Chem Technol Biot, 2013, 88(5):808-817.
[12] YOON S, HONG E, KIM S, et al. Optimization of culture medium for enhanced production of exopolysaccharide from Aureobasidium pullulans[J]. Bioproc Biosyst Eng, 2012, 35(1-2):167-172.
[13]叶陵.植物乳杆菌W-4的分离鉴定及其发酵剁辣椒研究[D].湖南:湖南农业大学,2017.
[14]钟燕青.自然发酵辣椒中乳酸菌分离筛选及香气成分分析[D].湖南:湖南农业大学,2012.
[15]王兰,赵玲艳,陈思思,等.发酵辣椒中产亚硝酸盐还原酶短乳杆菌L5的选育及特性研究[J].中国酿造,2014,33(11):25-29.
[16]王昌禄,韩晓梅,陈勉华,等. Viili中胞外多糖的分离纯化及对秀丽线虫寿命的影响[J].氨基酸和生物资源,2011,33(2):64-67.
[17] SAIJA N, WELMAN A D, BENNETT R J. Development of a dairybased exopolysaccharide bioingredient[J]. Int Dairy, 2010, 20(9):603-608.
[18]刘素纯,吕嘉枥,蒋立文.食品微生物学实验[M].北京:化学工业出版社,2013.
[19]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:中国农业出版社,1995.
[20] MOZZI F, ROLLAN G, Q SAVOY G DE DIORI, et al. Effect of galactose and glucose on the exopolysaccharide production and the activities of biosynthetic enzymes in Lactobacillus casei CRL 87[J]. J Appl Microbiol,2001, 91:160-167.