GLI1、p-STAT3蛋白在胆囊癌患者中的表达情况及与临床特征的关系
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摘要
目的探讨神经胶质瘤相关癌基因同源物1(GLI1)、磷酸化信号转导及转录激活因子3(p-STAT3)在胆囊癌患者中的表达情况及与临床特征的关系。方法选取60例胆囊癌患者和60例慢性胆囊炎患者,并取其胆囊癌组织标本和胆囊炎组织标本。采用免疫组织化学染色法检测两组标本中GLI1、p-STAT3蛋白的阳性表达率,分析不同临床特征胆囊癌患者胆囊癌组织中GLI1、p-STAT3蛋白的表达情况。结果胆囊癌组织中GLI1、pSTAT3蛋白的阳性表达率均明显高于胆囊炎组织,差异均有统计学意义(P﹤0.01)。不同分化程度、Nevin分期、淋巴结转移情况胆囊癌患者胆囊癌组织中GLI1、p-STAT3蛋白阳性表达率比较,差异均有统计学意义(P﹤0.05)。结论 GLI1、p-STAT3蛋白在胆囊癌组织中阳性表达率较高,且可能与胆囊癌的分化程度、Nevin分期和淋巴结转移有关。
Objective To investigate the expression of glioma-associated oncogene homolog 1(GLI1) and phosphorylated-signal transduction and activator of transcription 3(p-STAT3) in patients with gallbladder cancer, and to discuss the relationship with clinical characteristics. Method 60 patients with gallbladder carcinoma and 60 patients with chronic cholecystitis were collected, and specimens of gallbladder carcinoma and cholecystitis were taken. The expression of GLI1 and p-STAT3 were detected by immunohistochemistry staining. The expression of GLI1 and p-STAT3 in gallbladder tissues in patients with different clinical characteristics were analyzed. Result The positive expression rates of GLI1 protein and p-STAT3 protein in gallbladder cancer tissues were significantly higher than those in cholecystitis tissues(P<0.01). The positive expression rates of GLI1 protein and p-STAT3 protein in gallbladder cancer tissues were of significantly differences among patients with varied degree of differentiation, Nevin stages, and lymph node metastasis(P<0.05).Conclusion GLI1 and p-STAT3 are highly expressed in gallbladder cancer tissues, and are closely related to the degree of differentiation, Nevin stage and lymph node metastasis.
Objective To investigate the expression of glioma-associated oncogene homolog 1(GLI1) and phosphorylated-signal transduction and activator of transcription 3(p-STAT3) in patients with gallbladder cancer, and to discuss the relationship with clinical characteristics. Method 60 patients with gallbladder carcinoma and 60 patients with chronic cholecystitis were collected, and specimens of gallbladder carcinoma and cholecystitis were taken. The expression of GLI1 and p-STAT3 were detected by immunohistochemistry staining. The expression of GLI1 and p-STAT3 in gallbladder tissues in patients with different clinical characteristics were analyzed. Result The positive expression rates of GLI1 protein and p-STAT3 protein in gallbladder cancer tissues were significantly higher than those in cholecystitis tissues(P<0.01). The positive expression rates of GLI1 protein and p-STAT3 protein in gallbladder cancer tissues were of significantly differences among patients with varied degree of differentiation, Nevin stages, and lymph node metastasis(P<0.05).Conclusion GLI1 and p-STAT3 are highly expressed in gallbladder cancer tissues, and are closely related to the degree of differentiation, Nevin stage and lymph node metastasis.
引文
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[3]吴孟超,吴在德,吴肇汉,等.外科学(第八版)[M].北京:人民卫生出版社,2013:89-93.
[4]中华消化杂志编辑委员会,中华医学会消化病学分会肝胆疾病协作组.中国慢性胆囊炎、胆囊结石内科诊疗共识意见(2018年)[J].中华消化杂志,2019,39(2):73-79.
[5]李新星,滕世峰,徐楷,等.TROP2、p-ERK1/2和Cyclin D1在胆囊癌组织中的表达及临床意义[J].临床肝胆病杂志,2017,33(5):909-914.
[6]崔长青,吴迪,冯志强,等.CyclinD1和p16基因与胆结石相关胆囊癌发生机制关系的研究[J].中国现代普通外科进展,2014,17(7):527-530.
[7]依马木买买提江·阿布拉,李东伟,易超,等.Hedgehog信号通路基因Shh、Ptch1、Smo及Gli1在胰腺癌中的表达及意义[J].世界华人消化杂志,2015,23(18):2894-2900.
[8]吴旖琦,刘颖,赵丽,等.Hedgehog信号通路蛋白在肝内胆管细胞癌中的表达[J].国际免疫学杂志,2017,40(3):252-256.
[9]Zhou Z,Yao X,Pang S,et al.The deubiquitinase UCHL5/UCH37 positively regulates Hedgehog signaling by deubiquitinating smoothened[J].J Mol Cell Biol,2018,10(3):243-257.
[10]魏晰麟,周忠笑,张健,等.Hedgehog信号通路蛋白Ptch1和Gli1在胆管癌组织中的表达及意义[J].中国普外基础与临床杂志,2016,23(1):70-74.
[11]白明辉,孙君军,董玉宁,等.HMGB1在胆囊癌组织中的表达及意义[J].中国现代普通外科进展,2014,17(7):563-564.
[12]范跃祖,孙伟,张文忠,等.基质金属蛋白酶-1和膜型1基质金属蛋白酶在胆囊癌血管生成拟态中的表达及临床意义[J].外科研究与新技术,2014,3(1):8-16.
[13]Liao Q,Zeng Z,Guo X,et al.LPLUNC1 suppresses IL-6-induced nasopharyngeal carcinoma cell proliferation via inhibiting the Stat3 activation[J].Oncogene,2014,33(16):2098-2109.
[14]Xu S,Zhao N,Hui L,et al.MicroRNA-124-3p inhibits the growth and metastasis of nasopharyngeal carcinoma cells by targeting STAT3[J].Oncol Rep,2015,35(3):1385-1394.
[15]陆建华,李茂岚,李松岗,等.胞内氯离子通道蛋白1表达与胆囊癌侵袭转移的相关性研究[J].中华普通外科杂志,2013,28(2):112-115.