持续性与间歇性低载荷机械振动对成骨细胞增殖与分化的影响
摘要
目的观察不同低载荷振动方式对成骨细胞增殖与分化的影响。方法应用振动加载器给予培养的成骨细胞MC3T3-E1以强度为0.25 g、频率35 Hz的振动载荷,设置空白组(A组),持续振动10 min(B组)及振动5 min间歇5 min再振动5 min(C组)两种加载模式组,加载结束后分别检测各组MC3T3-E1细胞的增殖能力(CCK-8法)、碱性磷酸酶(ALP)活性及Runx-2蛋白的表达变化。结果 3组之间成骨细胞增殖情况无明显统计学意义(P=0.742),B组与C组ALP活性及Runx-2蛋白的表达明显高于A组,C组的ALP活性及Runx-2蛋白的表达显著高于B组(P<0.05)。结论低载荷机械振动对成骨细胞的增殖并无影响,然而低载荷机械振动可以促进成骨细胞的分化,特别是适当的间歇低载荷机械振动能够更加明显的促进成骨细胞的分化。
引文
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13 Zhou Y,Zhu ZL,Guan XX,et al.Reciprocal roles between caffeine and estrogen on bone via differently regulating c AMP/PKA pathway:the possible mechanism for caffeine-induced osteoporosis in women and estrogen's antagonistic effects〔J〕.Med Hypotheses,2009;73:83-5.
2 Pounder NM,Harrison AJ.Low intensity pulsed ultrasound for fracture healing:a review of the clinical evidence and the associated biological mechanism of action〔J〕.Ultrasonics,2008;48(4):330-8.
3 李丹智,张文杰.骨外固定器电刺激治疗骨不连接60例〔J〕.中国临床康复,2002;(2):237.
4 熊进,林华,骆东山.高能体外震波(ESWT)治疗长骨骨不连〔J〕.中国矫形外科杂志,2005;(20):16-8.
5 Ozcivici E,Luu YK,Rubin CT,et al.Low-level vibrations retain bone marrow's osteogenic potential and augment recovery of trabecular bone during reambulation〔J〕.PLo S One,2010;5(6):e11178.
6 Prisby RD,Lafage-Proust MH,Malaval L,et al.Effects of whole body vibration on the skeleton and other organ systems in man and animal models:what we know and what we need to know〔J〕.Ageing Res Rev,2008;7(4):319-29.
7 Wysocki A,Butler M,Shamliyan T,et al.Whole-body vibration therapy for osteoporosis:state of the science〔J〕.Ann Intern Med,2011;155(10):680-6,W206-13.
8 Rubin C,Turner AS,Bain S,et al.Low mechanical signals strengthen long bones〔J〕.Nature,2001;412(6847):603-4.
9 Rubin C,Recker R,Cullen D,et al.Prevention of postmenopausal bone loss by a low-magnitude,high-frequency mechanical stimuli:a clinical trial assessing compliance,efficacy,and safety〔J〕.J Bone Miner Res,2004;19(3):343-51.
10 关键,王健平,车笑非,等.缺氧条件对成骨细胞VEGF和TGF-β1表达影响的实验研究〔J〕.中国修复重建外科杂志,2008;22(8):984-8.
11 Ducy P,Zhang R,Geoffory V,et al.Osf2/Cbfa1:a transcriptional activator of osteoblast differentiation〔J〕.Cell,1997;89:747-54.
12 Teitelbaum SL.Bone resorption by osteoclasts〔J〕.Science,2000;289:1504-8.
13 Zhou Y,Zhu ZL,Guan XX,et al.Reciprocal roles between caffeine and estrogen on bone via differently regulating c AMP/PKA pathway:the possible mechanism for caffeine-induced osteoporosis in women and estrogen's antagonistic effects〔J〕.Med Hypotheses,2009;73:83-5.