中草药对大别山地区奶牛隐性乳腺炎病原菌的抑菌试验
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摘要
本试验通过对中草药的药性作用机理分析,筛选出23味中草药,并提取药物有效成分,对大别山地区奶牛隐性乳腺炎4种病原菌进行单味的药敏试验,旨在筛选出抑菌效果好的中草药,为中草药治疗奶牛隐性乳腺炎提供参考。本试验表明:白头翁、蒲公英、通草、女贞子、半枝莲和穿心莲对金黄色葡萄球菌有明显的抑菌效果,石榴皮对大肠杆菌有明显的抑菌效果,白头翁、半枝莲和地榆对沙雷氏菌有明显的抑菌效果,半枝莲和穿心莲对阴沟肠杆菌有明显的抑菌效果。
引文
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[2]ROBERSON J R,WARNICK L D,MOORE G.Mild to moderate clinical mastiis:efficacy of intramammary amoxicillin,frequent milk-out,a combined intramammary amoxicillin,and frequent milk-out treatment versus no treatment[J].Journal of Dairy Science,2004,87(3):583-592.
[3]WILSON D J,GONZALEZ R N,CASE K L,et al.Comparison of seven antibiotic treatments with no treatment for bacteriological efficacy against bovine mastitis pathogens[J].Journal of Dairy Science,1999,82(8):1664-1670.
[4]YUMAMOTO Y,OUE E.Antihypertensive effect of quercetin in rats fed with a high-fat high-sucrose diet[J].Journal of the Agricultural Chemical Society of Japan,2006,70(4):933-939.
[5]赵瑞芝,欧润妹,章新生,等.不同提取方法对大黄游离蒽醌含量及抑菌活性影响的研究[J].中国药学杂志,2001,36(7):483-484.
[6]古肄静,王丽娟.蒲公英总黄酮的提取及其抑菌性能[J].东北林业大学学报,2007,35(8):43-45.
[7]周广涛,高鹏,戴兵,等.木香中去氢木香内酯和木香烃内酯提取工艺优选[J].中国实验方剂学杂志,2013,19(2):40-42.
[8]刘靖岩,贾艾玲,邱智东.通草提取工艺及其鉴别的研究[J].江西中医药,2013,44(9):68-69.
[9]田永芝,王亚丽,孙茹,等.响应面法优化川楝子总黄酮的提取工艺[J].中国医药信息杂志,2016,23(4):76-79.
[10]陈象青,刘圣,方焱,等.多指标正交试验优选当归提取工艺[J].中国使用方剂学杂志,2010,16(8):25-28.
[11]YE Y N,LIU E S,Li Y,et al.Protective effect of polysaccharides-enriched fraction from Angelica sinensis on hepatic injury[J].Life Sciences,2001,69(6):637-646.
[12]白相国.栀子提取工艺的优化[J].科技资讯,2006(24):23.
[13]周玥.党参提取工艺的研究:全国中医药创新与发展研讨会专辑[C].昆明:中华中医药学会,2005.
[14]曲龙妹,赵春杰,李丹,等.正交试验优选白头翁汤提取工艺[J].中国药房,2009,20(12):904-906.
[15]张乐佳,夏新华.黄连提取工艺的研究[J].中成药,2001,23(6):398-400.
[16]吴立明.正交试验法考察黄芪的提取工艺[J].中国中医药信息杂志,2003,10(11):39-40.
[17]辛莉,黄桂东,钟先锋,等.生姜总黄酮提取工艺研究[J].中药材,2008,31(5):766-768.