544份水稻种质稻瘟病抗性鉴定及抗性基因的分布研究
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摘要
采用苗期喷雾接种鉴定方法并结合10个主效抗性基因的特异性分子标记检测,分析544份水稻种质资源的稻瘟病抗性水平及其携带的抗性基因分布情况。结果表明:稻瘟病抗性水平达高抗、抗、中抗、中感、感及高感的材料分别为25、50、78、152、156和83份;10个分子标记对应的抗病基因在供试材料中均被检测到,含有2和3个抗性基因的材料分别为4和17份(占0.7%和3.1%),476份材料含有4~6个抗性基因(占87.5%),47份材料含有7个抗性基因(占8.6%);品种抗病反应与其抗病基因种类密切相关,Pi5、Pita、Pi9、Pib等4个基因对6个强致病鉴定小种抗性表现较好。隆粳968、秀水134、嘉58、津稻263、淮稻20号、盐稻10号、谷梅4号等品种含有多个主效抗病基因,连续多年达到高抗水平。利用分子标记辅助选择将不同来源的主效抗病基因聚合到同一品种中,是控制该病害最经济有效的途径。
A total of 544 rice materials were assessed for blast resistance and resistance genes distribution by inoculation and screening of molecular markers tightly linked to 10 major resistance genes.Among the 544 materials,25,50,78,152,156 and 83 exhibited high resistance,resistance,moderate resistance,moderate susceptibility,susceptibility and high susceptibility,respectively.Ten resistance genes corresponding to markers were found in these materials;Two and three resistance genes were found in 4 and 17 materials(0.7% and 3.1%);Four to six resistance genes were found in 476 materials(87.5%);Seven resistance genes were found in 47 materials(8.6%).The resistance of all rice materials was significantly associated with resistance genes.Pi5,Pita,Pi9 and Pib exhibited high resistance to 6 physiological races.The majority of resistance genes were found in Longjing 968,Xiushui 134,Jia 58,Jindao 263,Huaidao 20,Yandao 10 and Gumei 4.Pyramiding different resistance genes in rice by marker-assistedselection was suggested as the most economical,effective,and environment friendly measure for controlling rice blast disease in this study.
A total of 544 rice materials were assessed for blast resistance and resistance genes distribution by inoculation and screening of molecular markers tightly linked to 10 major resistance genes.Among the 544 materials,25,50,78,152,156 and 83 exhibited high resistance,resistance,moderate resistance,moderate susceptibility,susceptibility and high susceptibility,respectively.Ten resistance genes corresponding to markers were found in these materials;Two and three resistance genes were found in 4 and 17 materials(0.7% and 3.1%);Four to six resistance genes were found in 476 materials(87.5%);Seven resistance genes were found in 47 materials(8.6%).The resistance of all rice materials was significantly associated with resistance genes.Pi5,Pita,Pi9 and Pib exhibited high resistance to 6 physiological races.The majority of resistance genes were found in Longjing 968,Xiushui 134,Jia 58,Jindao 263,Huaidao 20,Yandao 10 and Gumei 4.Pyramiding different resistance genes in rice by marker-assistedselection was suggested as the most economical,effective,and environment friendly measure for controlling rice blast disease in this study.
引文
[1]黄富,刘成元,谢戎,叶华智.水稻种质资源抗稻瘟病及其恢保特性评价[J].中国农业科学,2005,38(4):831-836Huang F,Liu C Y,Xie R,Ye H Z.Evaluation of blast resistance and restoring or remaining characteristics of rice germplasm resources[J].Scientia Agricultura Sinica,2005,38(4):831-836(in Chinese)
[2]Baker B,Zambryski P,Staskawicz B,Dinesh S P.Signaling in plant microbe interactions[J].Science,1997,276:726-733
[3]Zeigler R S,Leong S A,Teng P S.Rice Blast Disease[M].Oxon:CAB International,1994:321-331
[4]沈瑛,朱培良,袁筱萍,Levy M,Decker M,Talbot N,Hamer JE.中国稻瘟病菌的遗传多样性[J].植物病理学报,1993,23(4):309-313Shen Y,Zhu P L,Yuan X P,Levy M,Decker M,Talbot N,Hamer J E.Genetic diversity of rice blast fungus in China[J].Acta Phytopathologica Sinica,1993,23(4):309-313(in Chinese)
[5]吴建利,庄杰云,李德葆,郑康乐.水稻对稻瘟病抗性的分子生物学研究进展[J].中国水稻科学,1999,13(2):123-128Wu J L,Zhuang J Y,Li D B,Zheng K L.Progress on rice molecular biology of resistance to blast fungus[J].Chinese Journal of Rice Science,1999,13(2):123-128(in Chinese)
[6]田大刚,苏军,陈建民,胡昌泉,陈在杰,王锋.1092份水稻材料稻瘟病抗性鉴定及抗性标记分析[J].分子植物育种,2012,10(2):214-221Tian D G,Su J,Chen J M,Hu C Q,Chen Z J,Wang F.Evaluation of blast resistance and analysis of resistance markers of 1 092rice materials[J].Molecular Plant Breeding,2012,10(2):214-221(in Chinese)
[7]谢倩凤,郭建夫,杨仕华,陈志雄,程本义,黄永相,李伟.82份水稻种质资源的稻瘟病抗性评价与抗性基因鉴定[J].广东农业科学,2015,14:9Xie Q F,Guo J F,Yang S H,Chen Z X,Cheng B Y,Huang YX,Li W.Evaluation of blast resistance spectrum and identification of resistance genes in 82 rice germplasm resources[J].Guangdong Agricultural Sciences,2015,14:9(in Chinese)
[8]邱福林,王和和,陈洁,庄杰云,Hei L,程式华,吴建利.利用于水稻突变体大量筛选的DNA微量快速提取法[J].中国水稻科学,2006,20(3):329-332Qiu F L,Wang H H,Chen J,Zhuang J Y,Hei L,Cheng S H,Wu J L.A rapid DNA mini-prep extraction method for largescale rice mutant screening[J].Chinese Journal of Rice Science,2006,20(3):329-332(in Chinese)
[9]Jiang J S,Wang S P.Identification of a 118kb DNA fragment containing the locus of blast resistance gene Pi2(t)in rice[J].Molecular Genetics and Genomics,2008,268(2):249-252
[10]王宁.Pi5基因的分子标记辅助选育及转甘薯储藏蛋白基因的抗稻瘟病水稻新材料培育[D].杭州:浙江大学,2012Wang N.Development of the blast-resistant rice by molecular marker-assisted selection of the Pi5 gene and genetic transformation of the sporamin gene[D].Hangzhou:Zhejiang University,2012(in Chinese)
[11]Qu S H,Liu G F,Zhou B,Bellizzi M,Zeng L R,Dai L Y,Han B,Wang G L.The broad-spectrum blast resistance gene Pi9encodes a nucleotide-binding site leucine-rich-repeat protein and a member of a multigene family in rice[J].Genetics,2006,172(3):1901-1914
[12]曾晓珊,杨先锋,赵正洪,林菲,王玲,潘庆华.稻瘟病抗病基因Pia的抗性分析及精细定位[J].中国科学:生命科学,2011,41(1):70-77Zeng X S,Yang X F,Zhao Z H,Lin F,Wang L,Pan Q H.Characterization and fine mapping of rice blast resistance gene Pia[J].Science China:Life Sciences,2011,41(1):70-77(in Chinese)
[13]时克,雷财林,程治军,许兴涛,王久林,万建民.稻瘟病抗性基因Pita和Pib在我国水稻主栽品种中的分布[J].植物遗传资源学报,2009,10(1):21-26Shi K,Lei C L,Cheng Z J,Xu X T,Wang J L,Wan J M.Distribution of two blast resistance genes Pita and Pib in major rice cultivars in China[J].Journal of Plant Genetic Resources,2009,10(1):21-26(in Chinese)
[14]王忠华,贾育林,吴殿星,夏英武.水稻抗稻瘟病基因Pita的分子标记辅助选择[J].作物学报,2004,30(12):1259-1265Wang Z H,Jja Y L,Wu D X,Xia Y W.Molecular markers assisted selection of the rice blast resistance gene Pita[J].Acta Agronomica Sinica,2004,3(12):1259-1265(in Chinese)
[15]Chen X W,Li S G,Xu J C,Zhai W X,Ling Z Z,Ma B T,Wang Y P,Wang W M,Cao G,Ma Y Q,Shang J J,Zhao X F,Zhou KD,Zhu L H.Identification of two blast resistance genes in a rice variety Digu[J].Journal of Phytopathology,2004,152(2):77-85
[16]杨小林.湖北省稻瘟病重发区病菌群体致病性分化性及水稻抗稻瘟基因的分析[D].武汉:华中农业大学,2012Yang X L.Population of Magnaporthe grisea(Hebert)barr in rice blast epidemic areas of Hubei Province and genetic analysis of resistance gene to rice blast[D].Wuhan:Huazhong Agricultural University,2012(in Chinese)
[17]Deng Y W,Zhu X D,Shen Y,He Z H.Genetic characterization and fine mapping of the blast resistance locus Pigm(t)tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety[J].Theoretical and Applied Genetics,2006,113(4):705-713
[18]Akira T,Nagao H,Akio M,Hirohiko H.Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon tagging BMC[J].Plant Biology,2010,10:175
[19]涂军明,陈仕哲,吕锐玲.分子标记辅助选择技术在水稻稻瘟病抗性改良中的应用[J].江西农业学报,2012,24(10):55-58Tu J M,Chen S Z,Lv R L.Application of molecular markerassisted selection in rice blast resistance improvement[J].Acta Agriculturae Jiangxi,2012,24(10):55-58(in Chinese)
[20]朱金燕,王军,范方军,李文奇,王芳权,仲维功,杨杰.水稻稻瘟病广谱抗病新等位基因pi21t的鉴定及其抗性应用[J].华北农学报,2014,29(6):11-15Zhu J Y,Wang J,Fan F J,Li W Q,Wang F Q,Zhong W G,Yang J.Identification and application of one new rice blast broad-spectrum resistance allele pi21t[J].Acta Agriculturae Boreali-Simica,2014,29(6):11-15(in Chinese)
[21]刘辉,孟德龙,查日扬,徐大勇.江苏水稻品种稻瘟病主效抗性基因鉴定及应用评价[J].福建农业学报,2015,30(5):452-458Liu H,Meng D L,Zha R Y,Xu D Y.Identification and evaluation on blast resistance of rice varieties in Jiangsu[J].Fujian Journal of Agriculture Science,2015,30(5):452-458(in Chinese)
[22]陈能刚,陈惠查,阮仁超,游俊梅.水稻抗稻瘟病种质资源的研究进展[J].贵州农业科学,2010,38(12):7-10Chen N G,Chen H C,Ruan R C,You J M.Research progress in blast resistant germplasm resources in rice[J].Guizhou Agricultural Sciences,2010,38(12):7-10(in Chinese)
[23]张磊,张启军,程兆榜,杨豪,周益军,吕川根.4套水稻稻瘟病鉴别品种对江苏地区主要稻瘟病菌株的抗性分析[J].江苏农业学报,2010,26(5):948-953Zhang L,Zhang Q J,Cheng Z B,Yang H,Zhou Y J,Lv C G.Resistances of four sets of rice varieties to 19 strains of Magnaporthe griseas in Jiangsu area[J].Jiangsu Journal of Agriculture Science,2010,26(5):948-953(in Chinese)
[24]高汉亮,颜群.广西引种水稻抗稻瘟病鉴定[J].广西农学报,2006,21(4):17-19Gao H L,Han Q.Identification of anti-Magnaporthe griseain introduction of fine variety of rice in Guangxi[J].Journal of Guangxi Agriculture,2006,21(4):17-19(in Chinese)
[25]黄洪河,郑家团,郑常林.近五年福建省级审定水稻主要米质指标和稻瘟病抗性分析[J].中国稻米,2004,(1):18-20Huang H H,Zheng J T,Zheng C L.Analysis on the main quality index and the blast resistance of Fujian rice varieties nearly five years[J].China Rice,2004,(1):18-20(in Chinese)
[26]张锦文,谭亚玲,洪汝科,范静华,罗琼,曾千春.高原粳稻子预44抗稻瘟病基因遗传分析和定位[J].中国水稻科学,2009,23(1):31-35Zhang J W,Tan Y L,Hong R K,Fan J H,Luo Q,Zeng Q C.Genetic analysis and gene mapping of rice blast resistance in japonica variety Ziyu 44[J].Chinese Journal of Rice Science,2009,23(1):31-35(in Chinese)
[27]Zhuang J Y,Ma W B,Wu J L,Chai R Y,Lu J,Fan Y Y,Jin MZ,Leung H,Zheng K L.Mapping of leaf and neck blast resistance genes with resistance gene analog RAPD and RFLPin rice[J].Euphytica,2002,128(3):363-370
[2]Baker B,Zambryski P,Staskawicz B,Dinesh S P.Signaling in plant microbe interactions[J].Science,1997,276:726-733
[3]Zeigler R S,Leong S A,Teng P S.Rice Blast Disease[M].Oxon:CAB International,1994:321-331
[4]沈瑛,朱培良,袁筱萍,Levy M,Decker M,Talbot N,Hamer JE.中国稻瘟病菌的遗传多样性[J].植物病理学报,1993,23(4):309-313Shen Y,Zhu P L,Yuan X P,Levy M,Decker M,Talbot N,Hamer J E.Genetic diversity of rice blast fungus in China[J].Acta Phytopathologica Sinica,1993,23(4):309-313(in Chinese)
[5]吴建利,庄杰云,李德葆,郑康乐.水稻对稻瘟病抗性的分子生物学研究进展[J].中国水稻科学,1999,13(2):123-128Wu J L,Zhuang J Y,Li D B,Zheng K L.Progress on rice molecular biology of resistance to blast fungus[J].Chinese Journal of Rice Science,1999,13(2):123-128(in Chinese)
[6]田大刚,苏军,陈建民,胡昌泉,陈在杰,王锋.1092份水稻材料稻瘟病抗性鉴定及抗性标记分析[J].分子植物育种,2012,10(2):214-221Tian D G,Su J,Chen J M,Hu C Q,Chen Z J,Wang F.Evaluation of blast resistance and analysis of resistance markers of 1 092rice materials[J].Molecular Plant Breeding,2012,10(2):214-221(in Chinese)
[7]谢倩凤,郭建夫,杨仕华,陈志雄,程本义,黄永相,李伟.82份水稻种质资源的稻瘟病抗性评价与抗性基因鉴定[J].广东农业科学,2015,14:9Xie Q F,Guo J F,Yang S H,Chen Z X,Cheng B Y,Huang YX,Li W.Evaluation of blast resistance spectrum and identification of resistance genes in 82 rice germplasm resources[J].Guangdong Agricultural Sciences,2015,14:9(in Chinese)
[8]邱福林,王和和,陈洁,庄杰云,Hei L,程式华,吴建利.利用于水稻突变体大量筛选的DNA微量快速提取法[J].中国水稻科学,2006,20(3):329-332Qiu F L,Wang H H,Chen J,Zhuang J Y,Hei L,Cheng S H,Wu J L.A rapid DNA mini-prep extraction method for largescale rice mutant screening[J].Chinese Journal of Rice Science,2006,20(3):329-332(in Chinese)
[9]Jiang J S,Wang S P.Identification of a 118kb DNA fragment containing the locus of blast resistance gene Pi2(t)in rice[J].Molecular Genetics and Genomics,2008,268(2):249-252
[10]王宁.Pi5基因的分子标记辅助选育及转甘薯储藏蛋白基因的抗稻瘟病水稻新材料培育[D].杭州:浙江大学,2012Wang N.Development of the blast-resistant rice by molecular marker-assisted selection of the Pi5 gene and genetic transformation of the sporamin gene[D].Hangzhou:Zhejiang University,2012(in Chinese)
[11]Qu S H,Liu G F,Zhou B,Bellizzi M,Zeng L R,Dai L Y,Han B,Wang G L.The broad-spectrum blast resistance gene Pi9encodes a nucleotide-binding site leucine-rich-repeat protein and a member of a multigene family in rice[J].Genetics,2006,172(3):1901-1914
[12]曾晓珊,杨先锋,赵正洪,林菲,王玲,潘庆华.稻瘟病抗病基因Pia的抗性分析及精细定位[J].中国科学:生命科学,2011,41(1):70-77Zeng X S,Yang X F,Zhao Z H,Lin F,Wang L,Pan Q H.Characterization and fine mapping of rice blast resistance gene Pia[J].Science China:Life Sciences,2011,41(1):70-77(in Chinese)
[13]时克,雷财林,程治军,许兴涛,王久林,万建民.稻瘟病抗性基因Pita和Pib在我国水稻主栽品种中的分布[J].植物遗传资源学报,2009,10(1):21-26Shi K,Lei C L,Cheng Z J,Xu X T,Wang J L,Wan J M.Distribution of two blast resistance genes Pita and Pib in major rice cultivars in China[J].Journal of Plant Genetic Resources,2009,10(1):21-26(in Chinese)
[14]王忠华,贾育林,吴殿星,夏英武.水稻抗稻瘟病基因Pita的分子标记辅助选择[J].作物学报,2004,30(12):1259-1265Wang Z H,Jja Y L,Wu D X,Xia Y W.Molecular markers assisted selection of the rice blast resistance gene Pita[J].Acta Agronomica Sinica,2004,3(12):1259-1265(in Chinese)
[15]Chen X W,Li S G,Xu J C,Zhai W X,Ling Z Z,Ma B T,Wang Y P,Wang W M,Cao G,Ma Y Q,Shang J J,Zhao X F,Zhou KD,Zhu L H.Identification of two blast resistance genes in a rice variety Digu[J].Journal of Phytopathology,2004,152(2):77-85
[16]杨小林.湖北省稻瘟病重发区病菌群体致病性分化性及水稻抗稻瘟基因的分析[D].武汉:华中农业大学,2012Yang X L.Population of Magnaporthe grisea(Hebert)barr in rice blast epidemic areas of Hubei Province and genetic analysis of resistance gene to rice blast[D].Wuhan:Huazhong Agricultural University,2012(in Chinese)
[17]Deng Y W,Zhu X D,Shen Y,He Z H.Genetic characterization and fine mapping of the blast resistance locus Pigm(t)tightly linked to Pi2 and Pi9 in a broad-spectrum resistant Chinese variety[J].Theoretical and Applied Genetics,2006,113(4):705-713
[18]Akira T,Nagao H,Akio M,Hirohiko H.Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon tagging BMC[J].Plant Biology,2010,10:175
[19]涂军明,陈仕哲,吕锐玲.分子标记辅助选择技术在水稻稻瘟病抗性改良中的应用[J].江西农业学报,2012,24(10):55-58Tu J M,Chen S Z,Lv R L.Application of molecular markerassisted selection in rice blast resistance improvement[J].Acta Agriculturae Jiangxi,2012,24(10):55-58(in Chinese)
[20]朱金燕,王军,范方军,李文奇,王芳权,仲维功,杨杰.水稻稻瘟病广谱抗病新等位基因pi21t的鉴定及其抗性应用[J].华北农学报,2014,29(6):11-15Zhu J Y,Wang J,Fan F J,Li W Q,Wang F Q,Zhong W G,Yang J.Identification and application of one new rice blast broad-spectrum resistance allele pi21t[J].Acta Agriculturae Boreali-Simica,2014,29(6):11-15(in Chinese)
[21]刘辉,孟德龙,查日扬,徐大勇.江苏水稻品种稻瘟病主效抗性基因鉴定及应用评价[J].福建农业学报,2015,30(5):452-458Liu H,Meng D L,Zha R Y,Xu D Y.Identification and evaluation on blast resistance of rice varieties in Jiangsu[J].Fujian Journal of Agriculture Science,2015,30(5):452-458(in Chinese)
[22]陈能刚,陈惠查,阮仁超,游俊梅.水稻抗稻瘟病种质资源的研究进展[J].贵州农业科学,2010,38(12):7-10Chen N G,Chen H C,Ruan R C,You J M.Research progress in blast resistant germplasm resources in rice[J].Guizhou Agricultural Sciences,2010,38(12):7-10(in Chinese)
[23]张磊,张启军,程兆榜,杨豪,周益军,吕川根.4套水稻稻瘟病鉴别品种对江苏地区主要稻瘟病菌株的抗性分析[J].江苏农业学报,2010,26(5):948-953Zhang L,Zhang Q J,Cheng Z B,Yang H,Zhou Y J,Lv C G.Resistances of four sets of rice varieties to 19 strains of Magnaporthe griseas in Jiangsu area[J].Jiangsu Journal of Agriculture Science,2010,26(5):948-953(in Chinese)
[24]高汉亮,颜群.广西引种水稻抗稻瘟病鉴定[J].广西农学报,2006,21(4):17-19Gao H L,Han Q.Identification of anti-Magnaporthe griseain introduction of fine variety of rice in Guangxi[J].Journal of Guangxi Agriculture,2006,21(4):17-19(in Chinese)
[25]黄洪河,郑家团,郑常林.近五年福建省级审定水稻主要米质指标和稻瘟病抗性分析[J].中国稻米,2004,(1):18-20Huang H H,Zheng J T,Zheng C L.Analysis on the main quality index and the blast resistance of Fujian rice varieties nearly five years[J].China Rice,2004,(1):18-20(in Chinese)
[26]张锦文,谭亚玲,洪汝科,范静华,罗琼,曾千春.高原粳稻子预44抗稻瘟病基因遗传分析和定位[J].中国水稻科学,2009,23(1):31-35Zhang J W,Tan Y L,Hong R K,Fan J H,Luo Q,Zeng Q C.Genetic analysis and gene mapping of rice blast resistance in japonica variety Ziyu 44[J].Chinese Journal of Rice Science,2009,23(1):31-35(in Chinese)
[27]Zhuang J Y,Ma W B,Wu J L,Chai R Y,Lu J,Fan Y Y,Jin MZ,Leung H,Zheng K L.Mapping of leaf and neck blast resistance genes with resistance gene analog RAPD and RFLPin rice[J].Euphytica,2002,128(3):363-370