BB菌对赤泥pH的降低机理及改良效果探究
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摘要
降低赤泥pH是赤泥进行下一步利用和生态修复的重要前提。探索利用了耐盐碱菌株(Pannonibacter phragmitetus BB,以下简称BB菌)对赤泥进行改良。研究发现,BB菌主要通过产生有机酸的方式降低溶液及赤泥pH,最佳产酸条件为:葡萄糖15g/L、酵母膏9g/L、培养液与赤泥比例2∶1。震荡和静置培养时BB菌均产生草酸和柠檬酸,但震荡培养时BB菌还产生了乙酸。相关性分析显示,震荡培养时pH的下降主要是由草酸和乙酸引起的,而静置培养时主要是由草酸引起的。BB菌可有效降低赤泥pH,最佳条件下可将赤泥pH由11.71降至8.3左右。此外,BB菌的添加还可明显增加赤泥有机质的含量,最大增加幅度高达72.52%。
Reducing pH value of red mud is a significant prerequisite for utilization and ecological restoration of red mud.Improvement of red mud by saline-alkali tolerance tolerant bacterial(Pannonibacter phragmitetus BB)was explored.The results show that P.phragmitetus BB reduces pH value of red mud by producing organic acid.The optimum organic acid production conditions include glucose of 15 g/L,yeast extract of 9 g/L,ratio of culture solution to red mud of 2∶1.P.phragmitetus BB produces oxalic acid and citric acid under shaking and static condition,while P.phragmitetus BB also produces acetic acid under shaking condition.Correlation analysis shows that reduction of pH value of solution and red mud is mainly caused by oxalic acid and acetic acid in shaking culture;while reduction of pH value is mainly caused by oxalic acid when P.phragmitetus BB is cultured under static condition.Besides,P.phragmitetus BB reduces pH value of red mud from 11.71 to 8.3.
Reducing pH value of red mud is a significant prerequisite for utilization and ecological restoration of red mud.Improvement of red mud by saline-alkali tolerance tolerant bacterial(Pannonibacter phragmitetus BB)was explored.The results show that P.phragmitetus BB reduces pH value of red mud by producing organic acid.The optimum organic acid production conditions include glucose of 15 g/L,yeast extract of 9 g/L,ratio of culture solution to red mud of 2∶1.P.phragmitetus BB produces oxalic acid and citric acid under shaking and static condition,while P.phragmitetus BB also produces acetic acid under shaking condition.Correlation analysis shows that reduction of pH value of solution and red mud is mainly caused by oxalic acid and acetic acid in shaking culture;while reduction of pH value is mainly caused by oxalic acid when P.phragmitetus BB is cultured under static condition.Besides,P.phragmitetus BB reduces pH value of red mud from 11.71 to 8.3.
引文
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[2]朱军,兰建凯.赤泥的综合回收与利用[J].矿产保护与利用,2008(2):52-54.
[3]赵恒勤,金梅,蔡淑霞,等.石灰拜耳法赤泥沉降性能的研究[J].矿产保护与利用,2002(3):41-43.
[4]JONES B E H,HAYNES R J.Bauxite processing residue:a critical review of its formation,properties,storage,and revegetation[J].Critical Reviews in Environmental Science and Technology,2011,41(3):271-315.
[5]XUE S G,ZHU F,KONG X F,et al.A review of the characterization and revegetation of bauxite residues(red mud)[J].Environmental Science and Pollution Research,2016,23(2):1120-1132.
[6]王志,韩敏芳,张以河,等.拜耳法赤泥的湿法碳化脱碱工艺研究[J].硅酸盐通报,2013,32(9):1851-1855.
[7]李涛.赤泥堆场生态修复技术评价体系的研究[D].南昌:南昌大学,2013.
[8]张国立,李绍纯,张馨元,等.拜耳法赤泥水洗脱碱工艺的研究[J].青岛理工大学学报,2012,33(4):59-62.
[9]刘晓明,孙恒虎,冯向鹏,等.赤泥最佳热处理工艺制度研究[J].稀有金属材料与工程,2007,36(1):983-986.
[10]LEHOUX A P,LOCKWOOD C L,MAYES W M,et al.Gypsum addition to soils contaminated by red mud:implications for aluminium,arsenic,molybdenum and vanadium solubility[J].Environmental Geochemistry and Health,2013,35(5):643-656.
[11]任杰,刘继东,陈娟,等.醋渣和糠醛渣对赤泥中金属稳定性的影响[J].环境科学研究,2016,29(12):1895-1903.
[12]喻阳华,吴永贵,喻理飞,等.磷石膏与碳酸钙对赤泥脱碱的效果及可能机理[J].无机盐工业,2014,46(10):58-61.
[13]迟春明,王志春.客土改良对碱土饱和导水率与盐分淋洗的影响[J].农业系统科学与综合研究,2011,27(1):98-101.
[14]HAMDY M K,WILLIAMS F S.Bacterial amelioration of bauxite residue waste of industrial aluminaplants[J].Journal of Industrial Microbiology&Biotechnology,2001,27(4):228-233.
[15]KRISHNA P,REDDY M S,PATNAIK S K.Aspergillus tubingensis reduces the pH of the bauxite residue(red mud)amended soils[J].Water,Air,&Soil Pollution,2005,167(1):201-209.
[16]BALACHANDRAN C,DURAIPANDIYAN V,BALAKRISHNA K.Petroleumand polycyclic aromatic hydrocarbons(pAHs)degradation andnaphthalene metabolism in Streptomyces sp.(ERI-CpDA-1)isolated from oil contaminated soil[J].Bioresource Technology,2012,112:83-90.
[17]吴学玲,代沁芸,梁任星,等.利用高效降解菌株强化修复土壤中DBP及其细菌落动态解析[J].中南大学学报,2011,42(5):1188-1192.
[18]朱其瀚.镇江香醋发酵过程中微生物分离及其产酸特性[D].江苏无锡:江南大学,2008.
[19]余永建.镇江香醋有机酸组成及乳酸合成的生物强化[D].江苏无锡:江南大学,2014.
[20]秦忠雪,黎源倩,余倩.高效液相色谱法测定双歧杆菌的有机酸代谢产物[J].现代预防医学,2010,37(2):320-323.
[21]SANTINI T C,FEY M V.Spontaneous vegetation encroachment upon bauxite residue(red mud)as an indicator and facilitator of in situ remediation processes[J].Environmental Science&Technology,2013,47(21):12089-12096.