外加电场对原油微生物修复油污土壤的影响
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摘要
结合混合菌株与电场强化技术,对长庆油田油污土壤进行降解模拟实验。因不同电场强度刺激微生物产生的生物效应不同,表现出的底物降解效果也会有差异,故以0 V/m作为对比,探讨100 V/m,120 V/m,140 V/m,160 V/m电场强度对混合菌株生长繁殖和迁移特征的影响。实验表明,生长促进趋势先增后减,电场强度0～120 V/m表现为生长促进作用,并在120 V/m时达到最大值,140 V/m时几乎不受电场影响,而160 V/m则表现为生长抑制作用。将优选电场与混合菌株耦合技术应用到实际油污土壤样品修复中。结果表明,土壤中油污降解率最高达到90.2%;相比无电场作用,油污去除率提高了19.2%。
A simulated experiment on the degradation of oily soil in Changqing Oilfield is carried out with the combination of mixed strains and electric field enhancement technique. The biological effects of different electric field intensities on microorganisms are different, as well as the substrate degradation effects. Compared with 0 V/m, the effects of electric field strengths of 100 V/m, 120 V/m, 140 V/m and 160 V/m on the growth, reproduction and migration characteristics of mixed strains are investigated.The experimental curve shows that the growth promotion trend increases first and then decreases, and the electric field intensity 0～120 V/m,shows the growth promotion effect, and reaches the maximum at the time of 120 V/m; the mixed strain is almost unaffected by electric field at 140 V/m,while 160 V/m showes growth inhibition. The coupling technique of optimum electric field and mixed strain is applied to the remediation of oily soil samples. The results show that the highest degradation rate of oil pollution in soil is 90.2%, and the removal rate increases by 19.2% compared with that of no electric field.
A simulated experiment on the degradation of oily soil in Changqing Oilfield is carried out with the combination of mixed strains and electric field enhancement technique. The biological effects of different electric field intensities on microorganisms are different, as well as the substrate degradation effects. Compared with 0 V/m, the effects of electric field strengths of 100 V/m, 120 V/m, 140 V/m and 160 V/m on the growth, reproduction and migration characteristics of mixed strains are investigated.The experimental curve shows that the growth promotion trend increases first and then decreases, and the electric field intensity 0～120 V/m,shows the growth promotion effect, and reaches the maximum at the time of 120 V/m; the mixed strain is almost unaffected by electric field at 140 V/m,while 160 V/m showes growth inhibition. The coupling technique of optimum electric field and mixed strain is applied to the remediation of oily soil samples. The results show that the highest degradation rate of oil pollution in soil is 90.2%, and the removal rate increases by 19.2% compared with that of no electric field.
引文
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[2]陈立,张发旺,刘少玉,等.中原油田石油污染土壤原位生物修复技术实验研究[J].环境工程学报,2011,5(10):2385-2390.CHEN L,ZHANG F W,LIU S Y,et al.Experimental study on in-situ bioremediation technique for oil contaminated soil in Zhongyuan oil field[J].Chinese Journal of Environmental Engineering,2011,5(10):2385-2390.(in Chinese)
[3]郭倩瑜,张建民,李蓉蓉,等.含油废水处理中石油降解菌的筛选及其降解条件优化[J].纺织高校基础科学学报,2016,29(2):269-274.GUO Q Y,ZHANG J M,LI R R,et al.The screening of oil reduction bacteria and its optimal degradation conditions in oil wastewater[J].Basic Sciences Journal of Textile Universities,2016,29(2):269-274.(in Chinese)
[4]任建科,唐跃辉,赵敏.石油污染土壤微生物修复菌种选育研究[J].西安工程大学学报,2013,27(6):768-772.REN J K,TANG Y H,ZHAO M.The breeding of microbial remediation of petroleum-contaminated soil[J].Journal of Xi′an Polytechnic University,2013,27(6):768-772.(in Chinese)
[5]李杨,李凡修.石油污染土壤的微生物修复技术[J].化工环保,2017,37(6):605-610.LI Y,LI F X.Microbial remediation technology for petroleum contaminated soil[J].Environmental Protection of Chemical Industry,2017,37(6):605-610.(in Chinese)
[6]张建民,杨锐龙,陈希,等.改性载体固定化石油降解菌的制备及降解性能[J].纺织高校基础科学学报,2018,31(3):378-385.ZHANG J M,YANG R L,CHEN X,et al.Preparation and degradation of immobilized petroleum degrading bacteria with modified[J].Basic Sciences Journal of Textile Universities,2018,31(3):378-385.(in Chinese)
[7]张振新.电场对微生物的影响及电生物耦合处理有机废水的影响因素[J].建筑与预算,2017(12):38-40.ZHANG Z X.The influence of electric field on microorganism and the influence factors of electrobiological coupling treatment of organic wastewater[J].Construction and Budget,2017(12):38-40.(in Chinese)
[8]范瑞娟,郭书海,李凤梅,等.有机污染土壤电动-微生物修复过程中的影响因素及优化措施[J].生态环境学报,2017,26(3):522-530.FAN R J,GUO S H,LI F M,et al.Influencing factors and optimization measures in the electro-bioremediation of organic contaminated soil[J].Ecology and Environmental Sciences,2017,26(3):522-530.(in Chinese)
[9]LU L,HUGGINS T,JIN S,et al.Microbial metabolism and community structure in response to bioelectrochemically enhanced remediation of petroleum hydrocarbon-contaminated soil[J].Environmental Science&Technology,2014,48(7):4021-4029.
[10]郭长禄,赵彦宏,王艳芳,等.微生物降解石油烃类修复石油污染的研究[J].应用化工,2016,45(6):1155-1158.GUO C L,ZHAO Y H,WANG Y F,et al.Research on repairing oil pollution by hydrocarbon degradation microorganisms[J].Applied Chemical Industry,2016,45(6):1155-1158.(in Chinese)
[11]邱清华,哈尼帕,邓绍云.微生物降解石油烃类污染物的研究进展[J].资源开发与市场,2012,28(2):144-147.QIU Q H,HANIPA,DENG S Y.Research progress on microbial degradation of petroleum hydrocarbon[J].Resource Development&Market,2012,28(2):144-147.(in Chinese)
[12]张灿灿,郭书海,李婷婷,等.焦化厂高环PAHs污染土壤的电动-微生物修复[J].环境工程,2014,32(7):150-154.ZHANG C C,GUO S H,LI T T,et al.Electro-bioremediation of coking plant soils contaminated with high-molecular polycyclic aromatic hydrocarbons[J].Environmental Engineering,2014,32(7):150-154.(in Chinese)
[13]朱妍,赵敏,杨琴,等.电动力强化对微生物修复石油污染土壤的影响[J].油气田环境保护,2016,26(3):21-25.ZHU Y,ZHAO M,YANG Q,et al.Study on the influence of enhanced electrokinetic remediation on microbial remediation of oil contaminated soil[J].Environmental Protection of Oil&Gas Fields,2016,26(3):21-25.(in Chinese)
[14]孙先锋,金芬,高自文,等.电场对石油降解菌生长特性的影响[J].环境污染与防治,2015,37(10):48-51.SUN X F,JIN F,GAO Z W,et al.Effects of electric field on the growth characteristics of petroleum-degrading bacteria[J].Environmental Pollution&Control,2015,37(10):48-51.(in Chinese)
[15]李金洋,陈涛,胡艳清,等.外加直流电场对微生物燃料电池阳极微生物的影响研究[J].可再生能源,2012,30(4):93-96.LI J Y,CHEN T,HU Y Q,et al.Research on the anode bacterials of microbial fuel cell effcted by DC electric field[J].Renewable Energy Resources,2012,30(4):93-96.(in Chinese)
[16]中华人民共和国住房和城市建设部.CJ/T 221-2005城市污水处理厂污泥检测方法[S].北京:中国标准出版社,2006.Ministry of Housing and Urban-Rural Development of the People′s Republic of China(MOHURD).CJ/T 221-2005 Determination method for municipal sludge in wastewater treatment plant[S].Beijing:Standards Press of China,2006.(in Chinese)