响应面法优化西藏产卷丹多糖提取工艺
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摘要
本实验主要通过水提醇沉法对西藏产卷丹中的多糖成分进行提取并分析,通过在不同提取时间、提取温度以及料液比的情况下对多糖进行提取并分析各因素对提取效率的影响,最后以多糖提取得率作为评价指标,对提取效果进行评定。在单因素实验结果的基础之上,再通过Box-Behnken响应面法对卷丹提取条件进行优化处理。实验结果表明,多糖最佳的提取条件为:提取时间78.4 min、提取温度90℃、料液比1:29.4,在此最佳条件下进行多糖提取,其多糖得率为14.92%,与软件理论预期结果相差为0.18%。实验结果表明,利用响应面法优化卷丹多糖的提取工艺是合理可行的。
In this experiment, the polysaccharide components of Tibetan Lilium lancifolium were extracted and analyzed by water extraction and alcohol precipitation methods, and the effects of various factors on extraction efficiency were analyzed by extracting polysaccharide with different extraction time, extraction temperature and solid-liquid ratio. Finally, the extraction rate of polysaccharide was taken as the evaluation index to evaluate the extraction effect.On the basis of single factor experiment results, the extraction conditions of Lilium lancifolium were optimized by Box-Behnken response surface method. The experimental results showed that the best extraction condition of polysaccharide was extracting time 78.4 min,extraction temperature 90℃and solid-liquid ratio 1:29.4. Polysaccharide extraction was carried out under the optimal conditions, and the polysaccharide yield was 14.92%, which differed in 0.18% compared with the expected result of software theory. The experimental results showed that it was reasonable and feasible to optimize the extraction process of polysaccharide from Lilium lancifolium by response surface method.
In this experiment, the polysaccharide components of Tibetan Lilium lancifolium were extracted and analyzed by water extraction and alcohol precipitation methods, and the effects of various factors on extraction efficiency were analyzed by extracting polysaccharide with different extraction time, extraction temperature and solid-liquid ratio. Finally, the extraction rate of polysaccharide was taken as the evaluation index to evaluate the extraction effect.On the basis of single factor experiment results, the extraction conditions of Lilium lancifolium were optimized by Box-Behnken response surface method. The experimental results showed that the best extraction condition of polysaccharide was extracting time 78.4 min,extraction temperature 90℃and solid-liquid ratio 1:29.4. Polysaccharide extraction was carried out under the optimal conditions, and the polysaccharide yield was 14.92%, which differed in 0.18% compared with the expected result of software theory. The experimental results showed that it was reasonable and feasible to optimize the extraction process of polysaccharide from Lilium lancifolium by response surface method.
引文
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[8]刘剑,罗仓学,伍贤密,魏丽娜.响应面法优化金花葵籽多糖提取工艺研究[J].食品研究与开发,2018,39(01):25-29.
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[11]张秀红,孙静超,李琪.响应面法优化茉莉花茶茶多糖提取工艺[J].基因组学与应用生物学,2010,29(3):603-608.
[2]李卫民,盂宪舒.中药百合的研究概况[J].中草药,1991,22(6):277-278.
[3]傅春燕,刘永辉,李明娟,等.HPLC测定中药百合种2个甾体皂苷的含量[J].天然产物研究与开发,2012,24(9):1250-1252,1256.
[4]孙萍萍,孙剑锋,牟建楼.响应面法对缢蛏粗多糖提取工艺的优化[J].水产科学,2010,29(4):203-207.
[5]游庆红,尹秀莲.响应面法优化桑黄多糖的提取工艺研究[J].中国酿造,2010,218(5):67-69.
[6]魏桢元,钟耀广,刘长江.响应面优化法对香菇多糖提取的工艺研究[J].辽宁农业科学,2010(2):11-14.
[7]杨林莎,李玉贤,李明丽.苯酚-硫酸比色法测定百合多糖的含量[J].中国中医药信息杂志,2004(8):704-705.
[8]刘剑,罗仓学,伍贤密,魏丽娜.响应面法优化金花葵籽多糖提取工艺研究[J].食品研究与开发,2018,39(01):25-29.
[9]高丹丹,安文强,陈红.响应面法优化兰州百合多糖的提取工艺[J].食品工业科技,2013,34(05):226-229.
[10]钟葵,王强.响应面法优化龙眼多糖热水浸提的工艺[J].化工进展,2010,29(4):742-743.
[11]张秀红,孙静超,李琪.响应面法优化茉莉花茶茶多糖提取工艺[J].基因组学与应用生物学,2010,29(3):603-608.