转录因子CP2在结直肠癌中的表达及临床意义
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摘要
目的检测转录因子CP2(TFCP2)在结直肠癌中的表达,探讨其与结直肠癌临床病理特征之间的关系。方法以免疫组织化学法检测128例的结直肠癌组织以及癌旁结直肠黏膜组织中TFCP2的表达。采用Western blot、反转录-聚合酶链反应(RT-PCR)检测5例临床结直肠癌手术标本及癌旁结直肠黏膜组织中TFCP2的表达情况。结果免疫组织化学结果表明,TFCP2在结直肠癌组织的阳性表达率(85.9%)显著高于相应癌旁结直肠黏膜组织(21.1%)。TFCP2mRNA在前者的表达水平是后者的1.40～17.00倍。Western blot证实TFCP2在结直肠癌组织的表达是对应癌旁结直肠黏膜组织的1.01～3.56倍。TFCP2的表达与肿瘤患者发生的年龄、性别及肿瘤的部位等无关,与肿瘤的分化、浸润深度、侵袭转移、Dukes′分期等相关。结论 TFCP2在结直肠癌中呈现高表达,与结直肠癌患者的肿瘤临床病理特征有显著相关性。
Objective To detect transcription factor CP2(TFCP2)expression in colorectal carcinoma,and explore the relationship with the clinicopathological features.Methods The expression of TFCP2 was detected in 128 patients`colorectal carcinoma tissues and adjacent mucosal tissues by immunohistochemistry.The expression of TFCP2 colorectal carcinoma and adjacent mucosal tissues of 5 patients in surgical with colorectal carcinoma were detected by Western-blot and reverse transcription-PCR(RT-PCR).Results The immunohistochemistry results showed that the positive expression rate of TFCP2 in colorectal carcinoma tissues(85.9%)was significantly higher than that of the adjacent mucosal tissues(21.1%).The mRNA expression of TFCP2 was 1.40～17.00 times relative to colorectal tissue.Western blot confirmed that the expression of TFCP2 in colorectal carcinoma was 1.01～3.56 times that of adjacent tissues.TFCP2 expression was not related to the patient′s age of tumor onset,gender and location of the tumor,however,it is related to tumor differentiation,infiltration depth,invasion and metastasis and Dukes′stage.Conclusion TFCP2 highly expressed in colorectal carcinoma and there was a significant correlation with the clinicopathological features.
Objective To detect transcription factor CP2(TFCP2)expression in colorectal carcinoma,and explore the relationship with the clinicopathological features.Methods The expression of TFCP2 was detected in 128 patients`colorectal carcinoma tissues and adjacent mucosal tissues by immunohistochemistry.The expression of TFCP2 colorectal carcinoma and adjacent mucosal tissues of 5 patients in surgical with colorectal carcinoma were detected by Western-blot and reverse transcription-PCR(RT-PCR).Results The immunohistochemistry results showed that the positive expression rate of TFCP2 in colorectal carcinoma tissues(85.9%)was significantly higher than that of the adjacent mucosal tissues(21.1%).The mRNA expression of TFCP2 was 1.40～17.00 times relative to colorectal tissue.Western blot confirmed that the expression of TFCP2 in colorectal carcinoma was 1.01～3.56 times that of adjacent tissues.TFCP2 expression was not related to the patient′s age of tumor onset,gender and location of the tumor,however,it is related to tumor differentiation,infiltration depth,invasion and metastasis and Dukes′stage.Conclusion TFCP2 highly expressed in colorectal carcinoma and there was a significant correlation with the clinicopathological features.
引文
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[9]BRONIARCZYK J K,WAROWICKA A,KWASNIEWSKAA,et al.Expression of TSG101protein and LSF transcription factor in HPV-positive cervical cancer cells[J].Oncol Lett,2014,7(5):1409-1413.
[10]KAUR M,MACPHERSON C R,SCHMEIER S,et al.In silico discovery of transcription factors as potential diagnostic biomarkers of ovarian cancer[J].BMC Syst Biol,2011,5:144.
[11]CLEVERS H,NUSSE R.Wnt/β-catenin signaling and disease[J].Cell,2012,149(6):1192-1205.
[12]SCHINZARI V,TIMPERI E,PECORA G,et al.Wnt3a/β-Catenin signaling conditions differentiation of partially exhausted t-effector cells in human cancers[J].Cancer Immunol Res,2018,6(8):941-952.
[13]FEARON E R,VOGELSTEIN B.A genetic model for colorectal tumourigenesis[J].Cell,1990,61(5):759-767.
[2]KOTARBA G,KRZYWINSKA E,GRABOWSKA A I,et al.TFCP2/TFCP2L1/UBP1transcription factors in cancer[J].Cancer Lett,2018,420(420):72-79.
[3]DAI Y D,CAI Y,ZHAO J Y,et al.TFCP2activates betacatenin/TCF signaling in the progression of pancreatic cancer[J].Oncotarget,2017,8(41):70538-70549.
[4]GOTO Y,YAJIMA I,KUMASAKA M,et al.Transcription factor LSF(TFCP2)inhibits melanoma growth[J].Oncotarget,2016,7(3):2379-2390.
[5]AIGELSREITER A,RESS A L,BETTERMANN K,et al.Low expression of the putative tumour suppressor spinophilin is associated with higher proliferative activity and poor prognosis in patients with hepatocellular carcinoma[J].Br J Cancer,2013,108(9):1830-1837.
[6]KOKOSZYNSKA K,OSTROWSKI J,RYCHLEWSKIL,et al.The fold recognition of CP2transcription factors gives new insights into the function and evolution of tumor suppressor protein p53[J].Cell Cycle,2008,7(18):2907-2915.
[7]YOO B K,EMDAD L,GREDLER R,et al.Transcription factor Late SV40Factor(LSF)functions as an oncogene in hepatocellular carcinoma[J].Proc Natl Acad Sci U SA,2010,107(18):8357-8362.
[8]CHEN C H,TSAI H T,CHUANG H C,et al.Metformin disrupts malignant behavior of oral squamous cell carcinoma via a novel signaling involving Late SV40factor/Aurora-A[J].Sci Rep,2017,7(1):1358.
[9]BRONIARCZYK J K,WAROWICKA A,KWASNIEWSKAA,et al.Expression of TSG101protein and LSF transcription factor in HPV-positive cervical cancer cells[J].Oncol Lett,2014,7(5):1409-1413.
[10]KAUR M,MACPHERSON C R,SCHMEIER S,et al.In silico discovery of transcription factors as potential diagnostic biomarkers of ovarian cancer[J].BMC Syst Biol,2011,5:144.
[11]CLEVERS H,NUSSE R.Wnt/β-catenin signaling and disease[J].Cell,2012,149(6):1192-1205.
[12]SCHINZARI V,TIMPERI E,PECORA G,et al.Wnt3a/β-Catenin signaling conditions differentiation of partially exhausted t-effector cells in human cancers[J].Cancer Immunol Res,2018,6(8):941-952.
[13]FEARON E R,VOGELSTEIN B.A genetic model for colorectal tumourigenesis[J].Cell,1990,61(5):759-767.