非复杂妊娠新生儿游离肉碱浓度与孕龄相关性研究
|
摘要
目的探讨非复杂妊娠新生儿干血片中游离肉碱浓度与孕龄间的关系。方法回顾性分析2014年12月-2015年8月本院进行代谢筛查的非复杂性妊娠的8979例新生儿其肉碱检测结果。采用高效液相色谱-串联质谱检测所有新生儿足跟血3干血片中肉碱浓度,分析肉碱浓度与孕龄间的关系。结果早产儿肉碱浓度高于非早产儿(P<0.05)。新生儿体内肉碱浓度与孕龄呈负相关,r=-0.034,95%置信区间(-0.056,-0.014),(P=0.001)。结论随孕龄增加,胎儿脂肪合成增加而至足月后逐渐达到饱和,肉碱的利用反映了这种趋势。
Objective To investigate the relationship between concentration of free carnitine and neonatal gestational age in uncomplicated pregnancy. Methods Concentration of free carnitine among 8979 cases who underwent uncomplicated pregnancies in our hospital and were carried out neonatal screening between August 2014 and December2015 were analyzed retrospectively. Dry blood spot were collected on the third day of neonatal life,detection was conducted by high performance liquid tandem mass spectrometry(HPLC-MS). The relationship between concentration of carnitine and neonatal gestational age was analyzed by SPSS statistical analysis.Results The concentration of free carnitine in preterm birth infants was significantly higher than that in non-preterm birth infants(P<0.05). The concentration of carnitine was negatively correlated with neonatal gestational age,r=-0.034,95%CI(-0.056,-0.014),(P=0.001). Conclusion Metabolism of fetal fat synthesis increases gradually until full-term with the increase of gestational age,which can be reflected by the utilization of carnitine.
Objective To investigate the relationship between concentration of free carnitine and neonatal gestational age in uncomplicated pregnancy. Methods Concentration of free carnitine among 8979 cases who underwent uncomplicated pregnancies in our hospital and were carried out neonatal screening between August 2014 and December2015 were analyzed retrospectively. Dry blood spot were collected on the third day of neonatal life,detection was conducted by high performance liquid tandem mass spectrometry(HPLC-MS). The relationship between concentration of carnitine and neonatal gestational age was analyzed by SPSS statistical analysis.Results The concentration of free carnitine in preterm birth infants was significantly higher than that in non-preterm birth infants(P<0.05). The concentration of carnitine was negatively correlated with neonatal gestational age,r=-0.034,95%CI(-0.056,-0.014),(P=0.001). Conclusion Metabolism of fetal fat synthesis increases gradually until full-term with the increase of gestational age,which can be reflected by the utilization of carnitine.
引文
[1]Fafournoux P,Bruhat A,Jousse C.Amino acid regulation of gene expression[J].Biochem J.2000,351(Pt 1):1-12.
[2]van Sluijters D A,Dubbelhuis P F,Blommaart E F,et al.Amino-acid-dependent signal transduction[J].Biochem J.2000,351 Pt 3:545-550.
[3]Kilberg M S,Pan Y X,Chen H,et al.Nutritional control of gene expression:how mammalian cells respond to amino acid limitation[J].Annu Rev Nutr.2005,25:59-85.
[4]Millington D S,Norwood D L,Kodo N,et al.Application of fast atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in human urine,blood,and tissue[J].Anal Biochem.1989,180(2):331-339.
[5]Mchugh D,Cameron C A,Abdenur J E,et al.Clinical validation of cutoff target ranges in newborn screening of metabolic disorders by tandem mass spectrometry:a worldwide collaborative project[J].Genet Med.2011,13(3):230-254.
[6]Mandour I,El Gayar D,Amin M,et al.Amino Acid and Acylcarnitine Profiles in Premature Neonates:A Pilot Study[J].The Indian Journal of Pediatrics.2013,80(9):736-744.
[7]Nakatsukasa M,Sotozono C,Shimbo K,et al.Amino Acid Profiles in Human Tear Fluids Analyzed by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry[J].American Journal of Ophthalmology.2011,151(5):799-808.
[8]Turgeon C,Magera M J,Allard P,et al.Combined newborn screening for succinylacetone,amino acids,and acylcarnitines in dried blood spots[J].Clin Chem.2008,54(4):657-664.
[9]彭丽君,刘丽,彭敏芝,等.血浆酰基肉碱分析在遗传代谢缺陷病筛查诊断中的应用[J].实用儿科临床杂志.2012(20):1617-1620.
[10]陈伟岚,刘文丽,闫享福.糖尿病的药物治疗[J].新疆医学.2008(03):137-142.
[11]王志勇,王承军,何耀强.黄精赞育胶囊联合左卡尼丁口服液治疗男性不育症临床疗效观察[J].新疆医学.2012(10):98-99.
[12]Crill C M,Storm M C,Christensen M L,et al.Carnitine supplementation in premature neonates:effect on plasma and red blood cell total carnitine concentrations,nutrition parameters and morbidity[J].Clin Nutr.2006,25(6):886-896.
[13]Tsunoda M,Ikee R,Sasaki N,et al.Beneficial effects of combination therapy of intradialytic parenteral nutrition and oral L-carnitine administration[J].Int Urol Nephrol.2013,45(4):1235-1237.
[14]Clark R H,Chace D H,Spitzer A R.Impact of l-carnitine supplementation on metabolic profiles in premature infants[J].J Perinatol.2017,37(5):566-571.
[15]Seliger G,Kantelhardt E,VAN DER Wal C,et al.L-carnitine level in neonates a large,retrospective analysis[J].Arch Perinatal Med.2007(13):17-20.
[16]Meyburg J,Schulze A,Kohlmueller D,et al.Postnatal changes in neonatal acylcarnitine profile[J].Pediatr Res.2001,49(1):125-129.
[17]Giannacopoulou C,Evangeliou A,Matalliotakis I,et al.Effects of gestation age and of birth weight in the concentration of carnitine in the umbilical plasma[J].Clin Exp Obstet Gynecol.1998,25(1-2):42-45.
[18]Tokuriki S,Hayashi H,Okuno T,et al.Biotin and carnitine profiles in preterm infants in Japan[J].Pediatrics International.2013,55(3):342-345.
[19]韩田骏,林敏诗,郭勇.孕妇血浆游离肉碱水平与胎儿生长受限的相关性研究[J].广州医药.2014(03):13-15.
[20]Sanchez-Pintos P,Perez-Munuzuri A,Cocho J A,et al.Evaluation of carnitine deficit in very low birth weight preterm newborns small for their gestational age[J].J Matern Fetal Neonatal Med.2016,29(6):933-937.
[21]Liu Q,Wu J,Shen W,et al.Analysis of amino acids and acyl carnitine profiles in low birth weight,preterm,and small for gestational age neonates[J].JMatern Fetal Neonatal Med.2017:1-8.
[22]Meyburg J,Schmidt K G,Nutzenadel W,et al.Tuberculous pericarditis in an infant evolving during triple chemotherapy[J].Eur J Pediatr.2002,161(3):138-141.
[2]van Sluijters D A,Dubbelhuis P F,Blommaart E F,et al.Amino-acid-dependent signal transduction[J].Biochem J.2000,351 Pt 3:545-550.
[3]Kilberg M S,Pan Y X,Chen H,et al.Nutritional control of gene expression:how mammalian cells respond to amino acid limitation[J].Annu Rev Nutr.2005,25:59-85.
[4]Millington D S,Norwood D L,Kodo N,et al.Application of fast atom bombardment with tandem mass spectrometry and liquid chromatography/mass spectrometry to the analysis of acylcarnitines in human urine,blood,and tissue[J].Anal Biochem.1989,180(2):331-339.
[5]Mchugh D,Cameron C A,Abdenur J E,et al.Clinical validation of cutoff target ranges in newborn screening of metabolic disorders by tandem mass spectrometry:a worldwide collaborative project[J].Genet Med.2011,13(3):230-254.
[6]Mandour I,El Gayar D,Amin M,et al.Amino Acid and Acylcarnitine Profiles in Premature Neonates:A Pilot Study[J].The Indian Journal of Pediatrics.2013,80(9):736-744.
[7]Nakatsukasa M,Sotozono C,Shimbo K,et al.Amino Acid Profiles in Human Tear Fluids Analyzed by High-Performance Liquid Chromatography and Electrospray Ionization Tandem Mass Spectrometry[J].American Journal of Ophthalmology.2011,151(5):799-808.
[8]Turgeon C,Magera M J,Allard P,et al.Combined newborn screening for succinylacetone,amino acids,and acylcarnitines in dried blood spots[J].Clin Chem.2008,54(4):657-664.
[9]彭丽君,刘丽,彭敏芝,等.血浆酰基肉碱分析在遗传代谢缺陷病筛查诊断中的应用[J].实用儿科临床杂志.2012(20):1617-1620.
[10]陈伟岚,刘文丽,闫享福.糖尿病的药物治疗[J].新疆医学.2008(03):137-142.
[11]王志勇,王承军,何耀强.黄精赞育胶囊联合左卡尼丁口服液治疗男性不育症临床疗效观察[J].新疆医学.2012(10):98-99.
[12]Crill C M,Storm M C,Christensen M L,et al.Carnitine supplementation in premature neonates:effect on plasma and red blood cell total carnitine concentrations,nutrition parameters and morbidity[J].Clin Nutr.2006,25(6):886-896.
[13]Tsunoda M,Ikee R,Sasaki N,et al.Beneficial effects of combination therapy of intradialytic parenteral nutrition and oral L-carnitine administration[J].Int Urol Nephrol.2013,45(4):1235-1237.
[14]Clark R H,Chace D H,Spitzer A R.Impact of l-carnitine supplementation on metabolic profiles in premature infants[J].J Perinatol.2017,37(5):566-571.
[15]Seliger G,Kantelhardt E,VAN DER Wal C,et al.L-carnitine level in neonates a large,retrospective analysis[J].Arch Perinatal Med.2007(13):17-20.
[16]Meyburg J,Schulze A,Kohlmueller D,et al.Postnatal changes in neonatal acylcarnitine profile[J].Pediatr Res.2001,49(1):125-129.
[17]Giannacopoulou C,Evangeliou A,Matalliotakis I,et al.Effects of gestation age and of birth weight in the concentration of carnitine in the umbilical plasma[J].Clin Exp Obstet Gynecol.1998,25(1-2):42-45.
[18]Tokuriki S,Hayashi H,Okuno T,et al.Biotin and carnitine profiles in preterm infants in Japan[J].Pediatrics International.2013,55(3):342-345.
[19]韩田骏,林敏诗,郭勇.孕妇血浆游离肉碱水平与胎儿生长受限的相关性研究[J].广州医药.2014(03):13-15.
[20]Sanchez-Pintos P,Perez-Munuzuri A,Cocho J A,et al.Evaluation of carnitine deficit in very low birth weight preterm newborns small for their gestational age[J].J Matern Fetal Neonatal Med.2016,29(6):933-937.
[21]Liu Q,Wu J,Shen W,et al.Analysis of amino acids and acyl carnitine profiles in low birth weight,preterm,and small for gestational age neonates[J].JMatern Fetal Neonatal Med.2017:1-8.
[22]Meyburg J,Schmidt K G,Nutzenadel W,et al.Tuberculous pericarditis in an infant evolving during triple chemotherapy[J].Eur J Pediatr.2002,161(3):138-141.