体外胚胎培养是胚胎工程技术的关键环节
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摘要
背景:体外早期胚胎的培养是胚胎工程技术的主要环节之一。目的:近年来,随着科学家的研究对胚胎发育和体外胚胎培养有了新认识和进展。方法:由第一作者检索1990年1月至2018年10月PubMed数据库相关文献,检索词为"Embryo Engineering;Pre-implantation Embryo;In vitro embryo culture;Assisted Reproductive Technology;ART",文献语言限制在英文。根据纳入排除标准,最终纳入76条进行分析探讨。结果与结论:在严格遵循医学伦理学原则条件下开展的胚胎体外培养体系是动物胚胎工程的一项关键技术,是保证运用胚胎生物技术顺利生产体外胚胎的基础,通过改良体外胚胎培养体系,可以使体外培养环境更加接近体内环境,提高体外胚胎质量。
BACKGROUND: In vitro embryo culture is one of the key links to animal embryo engineering technology.OBJECTIVE: To review the recent progress in embryonic development and embryo culture in vitro.METHODS: PubMed database was searched for relevant articles published from January 1990 to October 2018. The keywords were "embryo engineering; pre-implantation embryo; in vitro embryo culture; assisted reproductive technology, ART" in English. Finally, 76 eligible articles were included in result analysis.RESULTS AND CONCLUSION: In vitro embryo culture system is a key technology of animal embryo engineering that is used in strict accordance with the medical ethical requirements. This culture system is essential for the smooth production of in vitro embryos using embryonic biotechnology. An improved in vitro embryo culture system is conductive to establishing an in vitro culture environment being closer to the internal environment, thereby enhancing the quality of embryos.
BACKGROUND: In vitro embryo culture is one of the key links to animal embryo engineering technology.OBJECTIVE: To review the recent progress in embryonic development and embryo culture in vitro.METHODS: PubMed database was searched for relevant articles published from January 1990 to October 2018. The keywords were "embryo engineering; pre-implantation embryo; in vitro embryo culture; assisted reproductive technology, ART" in English. Finally, 76 eligible articles were included in result analysis.RESULTS AND CONCLUSION: In vitro embryo culture system is a key technology of animal embryo engineering that is used in strict accordance with the medical ethical requirements. This culture system is essential for the smooth production of in vitro embryos using embryonic biotechnology. An improved in vitro embryo culture system is conductive to establishing an in vitro culture environment being closer to the internal environment, thereby enhancing the quality of embryos.
引文
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[43]Swain JE,Carrell D,Cobo A,et al.Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential.Fertil Steril.2016;105(3):571-587.
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[45]Kane MT.The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro.J Exp Zool.1988;245(2):220-223.
[46]Carolan C,Lonergan P,Van Langendonckt A,et al.Factors affecting bovine embryo development in synthetic oviduct fluid following oocyte maturation and fertilization in vitro.Theriogenology.1995;43(6):1115-1128.
[47]Sagirkaya H,Misirlioglu M,Kaya A,et al.Developmental potential of bovine oocytes cultured in different maturation and culture conditions.Anim Reprod Sci.2007;101(3-4):225-240.
[48]Li XX,Cao PH,Han WX,et al.Non-invasive metabolomic profiling of culture media of ICSI-and IVF-derived early developmental cattle embryos via Raman spectroscopy.Anim Reprod Sci.2018;196:99-110.
[49]Youssef MM,Mantikou E,van Wely M,et al.Culture media for human pre-implantation embryos in assisted reproductive technology cycles.Cochrane Database Syst Rev.2015;(11):CD007876.
[50]Mattioli M,Galeati G,Barboni B,et al.Concentration of cyclic AMPduring the maturation of pig oocytes in vivo and in vitro.J Reprod Fertil.1994;100(2):403-409.
[51]Nureddin A,Epsaro E,Kiessling AA.Purines inhibit the development of mouse embryos in vitro.J Reprod Fertil.1990;90(2):455-464.
[52]Loutradis D,John D,Kiessling AA.Hypoxanthine causes a 2-cell block in random-bred mouse embryos.Biol Reprod.1987;37(2):311-316.
[53]Bouillon C,Léandri R,Desch L,et al.Does Embryo Culture Medium Influence the Health and Development of Children Born after In Vitro Fertilization?PLoS One.2016;11(3):e0150857.
[54]Kane MT,Carney EW,Ellington JE.The role of nutrients,peptide growth factors and co-culture cells in development of preimplantation embryos in vitro.Theriogenology.1992;38(2):297-313.
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[56]Li X,Xu Y,Fu J,et al.Non-invasive metabolomic profiling of embryo culture media and morphology grading to predict implantation outcome in frozen-thawed embryo transfer cycles.J Assist Reprod Genet.2015;32(11):1597-1605.
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[59]Shi F,Li H,Wang E,et al.Melatonin reduces two-cell block via nonreceptor pathway in mice.J Cell Biochem.2018;119(11):9380-9393.
[60]Mishra A,Reddy IJ,Gupta P,et al.Developmental regulation and modulation of apoptotic genes expression in sheep oocytes and embryos cultured in vitro with L-carnitine.Reprod Domest Anim.2016;51(6):1020-1029.
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[2]Day BN.Reproductive biotechnologies:current status in porcine reproduction.Anim Reprod Sci.2000;60-61:161-72.
[3]Simopoulou M,Sfakianoudis K,Rapani A,et al.Considerations Regarding Embryo Culture Conditions:From Media to Epigenetics.In Vivo.2018;32(3):451-460.
[4]Wale PL,Gardner DK.The effects of chemical and physical factors on mammalian embryo culture and their importance for the practice of assisted human reproduction.Hum Reprod Update.2016;22(1):2-22.
[5]Chronopoulou E,Harper JC.IVF culture media:past,present and future.Hum Reprod Update.2015;21(1):39-55.
[6]Bowman P,McLaren A.Cleavage rate of mouse embryos in vivo and in vitro.J Embryol Exp Morphol.1970;24(1):203-207.
[7]Sinclair KD,Young LE,Wilmut I,et al.In-utero overgrowth in ruminants following embryo culture:lessons from mice and a warning to men.Hum Reprod.2000;15 Suppl 5:68-86.
[8]Otsuki J,Nagai Y,Matsuyama Y,et al.The redox state of recombinant human serum albumin and its optimal concentration for mouse embryo culture.Syst Biol Reprod Med.2013;59(1):48-52.
[9]Hammond J Jr.Recovery and culture of tubal mouse ova.Nature.1949;163(4131):28.
[10]Biggers JD,Gwatkin RB,Brinster RL.Development of mouse embryos in organ cultures of fallopian tubes on a chemically defined medium.Nature.1962;194:747-749.
[11]Papaioannou VE,Ebert KM.Development of fertilized embryos transferred to oviducts of immature mice.J Reprod Fertil.1986;76(2):603-608.
[12]Minami N,Bavister BD,Iritani A.Development of hamster two-cell embryos in the isolated mouse oviduct in organ culture system.Gamete Res.1988;19(3):235-240.
[13]Krisher RL,Petters RM,Johnson BH,et al.Development of porcine embryos from the one-cell stage to blastocyst in mouse oviducts maintained in organ culture.J Exp Zool.1989;249(2):235-239.
[14]Sirard MA,Lambert RD,Guay P,et al.In vivo and in vitro development of in vitro fertilized bovie follicular oocytes obtained by laparoscopy.Theriogenology.1985;23(1):230.
[15]Ueda SKM,Shioya Y,Hanada A.Culture of in vitro fertilized bovine embyros in a chemically defined medium(BO-medium).Jpn J Anim Reprod.1988;34:5.
[16]Sharif HVE.Development of early bovine embyros in mouse oviducts maintained in organ culture.Theriogenology.1991;35:270.
[17]Leese HJ.The formation and function of oviduct fluid.J Reprod Fertil.1988;82(2):843-856.
[18]Allen RL,Wright RW Jr.In vitro development of porcine embryos in coculture with endometrial cell monolayers or culture supernatants.JAnim Sci.1984;59(6):1657-1661.
[19]McCaffrey C,McEvoy TG,Diskin MG,et al.Successful co-culture of1-4-cell cattle ova to the morula or blastocyst stage.J Reprod Fertil.1991;92(1):119-124.
[20]Batista M,Torres A,Diniz P,et al.Development of a bovine luteal cell in vitro culture system suitable for co-culture with early embryos.In Vitro Cell Dev Biol Anim.2012;48(9):583-592.
[21]Lin S,Li R,Zheng X,et al.Influence of embryo culture medium on incidence of ectopic pregnancy in in vitro fertilization.Fertil Steril.2015;104(6):1442-1445.
[22]Gardner DK,Lane M.Culture and selection of viable blastocysts:a feasible proposition for human IVF?Hum Reprod Update.1997;3(4):367-382.
[23]Sirard MA.The influence of in vitro fertilization and embryo culture on the embryo epigenetic constituents and the possible consequences in the bovine model.J Dev Orig Health Dis.2017;8(4):411-417.
[24]López-Pelayo I,Gutiérrez-Romero JM,Armada AIM,et al.Comparison of two commercial embryo culture media(SAGE-1 step single medium vs.G1-PLUSTM/G2-PLUSTM sequential media):Influence on in vitro fertilization outcomes and human embryo quality.JBRA Assist Reprod.2018;22(2):128-133.
[25]Kamath MS,Mascarenhas M,Kirubakaran R,et al.Use of embryo culture supernatant to improve clinical outcomes in assisted reproductive technology:a systematic review and meta-analysis.Hum Fertil(Camb).2018;21(2):90-97.
[26]Brinster RL.Studies on the development of mouse embryos in vitro.IV.Interaction of energy sources.J Reprod Fertil.1965;10(2):227-240.
[27]Schini SA,Bavister BD.Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose.Biol Reprod.1988;39(5):1183-1192.
[28]Brown JJ,Whittingham DG.The dynamic provision of different energy substrates improves development of one-cell random-bred mouse embryos in vitro.J Reprod Fertil.1992;95(2):503-511.
[29]Brackett BG,Zuelke KA.Analysis of factors involved in the in vitro production of bovine embyros.Theriogenology.1993;39(1):43-64.
[30]Petters RM,Johnson BH,Reed ML,et al.Glucose,glutamine and inorganic phosphate in early development of the pig embryo in vitro.JReprod Fertil.1990;89(1):269-275.
[31]Khurana NK,Niemann H.Energy metabolism in preimplantation bovine embryos derived in vitro or in vivo.Biol Reprod.2000;62(4):847-856.
[32]Lane M,Gardner DK.Lactate regulates pyruvate uptake and metabolism in the preimplantation mouse embryo.Biol Reprod.2000;62(1):16-22.
[33]Johnson MH,Nasr-Esfahani MH.Radical solutions and cultural problems:could free oxygen radicals be responsible for the impaired development of preimplantation mammalian embryos in vitro?Bioessays.1994;16(1):31-38.
[34]Schini SA,Bavister BD.Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose.Biol Reprod.1988;39(5):1183-1192.
[35]Chatot CL,Ziomek CA,Bavister BD,et al.An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro.J Reprod Fertil.1989;86(2):679-688.
[36]Lane M,Gardner DK.Increase in postimplantation development of cultured mouse embryos by amino acids and induction of fetal retardation and exencephaly by ammonium ions.J Reprod Fertil.1994;102(2):305-312.
[37]Ludwig TE,Squirrell JM,Palmenberg AC,et al.Relationship between development,metabolism,and mitochondrial organization in 2-cell hamster embryos in the presence of low levels of phosphate.Biol Reprod.2001;65(6):1648-1654.
[38]Bedzhov I,Graham SJ,Leung CY,et al.Developmental plasticity,cell fate specification and morphogenesis in the early mouse embryo.Philos Trans R Soc Lond B Biol Sci.2014;369(1657):20130538.
[39]Kirkegaard K,Ahlstr?m A,Ingerslev HJ,et al.Choosing the best embryo by time lapse versus standard morphology.Fertil Steril.2015;103(2):323-332.
[40]Rose-Hellekant TA,Libersky-Williamson EA,Bavister BD.Energy substrates and amino acids provided during in vitro maturation of bovine oocytes alter acquisition of developmental competence.Zygote.1998;6(4):285-294.
[41]Bavister BD,Arlotto T.Influence of single amino acids on the development of hamster one-cell embryos in vitro.Mol Reprod Dev.1990;25(1):45-51.
[42]Spindle AI,Pedersen RA.Hatching,attachment,and outgrowth of mouse blastocysts in vitro:fixed nitrogen requirements.J Exp Zool.1973;186(3):305-318.
[43]Swain JE,Carrell D,Cobo A,et al.Optimizing the culture environment and embryo manipulation to help maintain embryo developmental potential.Fertil Steril.2016;105(3):571-587.
[44]Olson SE,Seidel GE Jr.Culture of in vitro-produced bovine embryos with vitamin E improves development in vitro and after transfer to recipients.Biol Reprod.2000;62(2):248-252.
[45]Kane MT.The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro.J Exp Zool.1988;245(2):220-223.
[46]Carolan C,Lonergan P,Van Langendonckt A,et al.Factors affecting bovine embryo development in synthetic oviduct fluid following oocyte maturation and fertilization in vitro.Theriogenology.1995;43(6):1115-1128.
[47]Sagirkaya H,Misirlioglu M,Kaya A,et al.Developmental potential of bovine oocytes cultured in different maturation and culture conditions.Anim Reprod Sci.2007;101(3-4):225-240.
[48]Li XX,Cao PH,Han WX,et al.Non-invasive metabolomic profiling of culture media of ICSI-and IVF-derived early developmental cattle embryos via Raman spectroscopy.Anim Reprod Sci.2018;196:99-110.
[49]Youssef MM,Mantikou E,van Wely M,et al.Culture media for human pre-implantation embryos in assisted reproductive technology cycles.Cochrane Database Syst Rev.2015;(11):CD007876.
[50]Mattioli M,Galeati G,Barboni B,et al.Concentration of cyclic AMPduring the maturation of pig oocytes in vivo and in vitro.J Reprod Fertil.1994;100(2):403-409.
[51]Nureddin A,Epsaro E,Kiessling AA.Purines inhibit the development of mouse embryos in vitro.J Reprod Fertil.1990;90(2):455-464.
[52]Loutradis D,John D,Kiessling AA.Hypoxanthine causes a 2-cell block in random-bred mouse embryos.Biol Reprod.1987;37(2):311-316.
[53]Bouillon C,Léandri R,Desch L,et al.Does Embryo Culture Medium Influence the Health and Development of Children Born after In Vitro Fertilization?PLoS One.2016;11(3):e0150857.
[54]Kane MT,Carney EW,Ellington JE.The role of nutrients,peptide growth factors and co-culture cells in development of preimplantation embryos in vitro.Theriogenology.1992;38(2):297-313.
[55]Flood MR,Gage TL,Bunch TD.Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro.Theriogenology.1993;39(4):823-833.
[56]Li X,Xu Y,Fu J,et al.Non-invasive metabolomic profiling of embryo culture media and morphology grading to predict implantation outcome in frozen-thawed embryo transfer cycles.J Assist Reprod Genet.2015;32(11):1597-1605.
[57]Rieger D,Luciano AM,Modina S,et al.The effects of epidermal growth factor and insulin-like growth factor I on the metabolic activity,nuclear maturation and subsequent development of cattle oocytes in vitro.J Reprod Fertil.1998;112(1):123-130.
[58]Swain JE.Optimal human embryo culture.Semin Reprod Med.2015;33(2):103-117.
[59]Shi F,Li H,Wang E,et al.Melatonin reduces two-cell block via nonreceptor pathway in mice.J Cell Biochem.2018;119(11):9380-9393.
[60]Mishra A,Reddy IJ,Gupta P,et al.Developmental regulation and modulation of apoptotic genes expression in sheep oocytes and embryos cultured in vitro with L-carnitine.Reprod Domest Anim.2016;51(6):1020-1029.
[61]Takahashi M,Honda T,Hatoya S,et al.Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture.J Vet Med Sci.2018;80(3):532-535.
[62]Highet AR,Bianco-Miotto T,Pringle KG,et al.A novel embryo culture media supplement that improves pregnancy rates in mice.Reproduction.2017;153(3):327-340.
[63]Borland RM,Biggers JD,Lechene CP,et al.Elemental composition of fluid in the human Fallopian tube.J Reprod Fertil.1980;58(2):479-482.
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